• The Korea Journal of Herbology
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• v.28 no.4
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• pp.17-23
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• 2013

Objectives : The objective of the paper was to study cutting method of Gardeniae frutus, that tends to show symptom of separations in a packing, in accordance with the comparison experiment of quantification and antioxidant effect proceed with the intact Gardeniae fructus(Gf), seed of Gardeniae fructus (Gs) and the pericarp of Gardeniae fructus (Gp) separately. Methods : The Gf, Gs, and Gp were extracted using 80% MeOH, followed by quantizing geniposide contained in each group. A MTT assay was conducted and ROS generation and NO production were measured for comparing its antioxidant effect. Results : As a result of quantizing geniposide contained in the Gf, Gp, and Gs, respectively, the geniposide content was shown to be the highest in the Gs. MTT assay showed that no cytotoxicity was observed in the groups treated with Gf, Gp, and Gs, respectively, at a dose of 500 ${\mu}g/ml$. The ROS generation was shown to have more significantly decreased in the group pretreated with Gf 500 ${\mu}g/ml$ than in the group treated with LPS. The NO level was shown to have more significantly decreased in the group pretreated with Gp 500 ${\mu}g/ml$ than in the group treated with LPS. Conclusion : As the geniposide content and antioxidant effect of Gf varies according to its each part, it is recommended that Gf should be distributed as an intact form other than segregation in packing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.5 no.1
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• pp.37-43
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• 1969

A genetrc study was made on plant height of indica rices with a few segregating F2 populations involving three semi-dwarf varieties such as T(N)I, CP231-SLO17, and B569A12. These populations were grown in breeding nursery at the International Rice Research Institute (IRRI) during several seasons. 20 to 25 day old seedlings grown at upland seedbed were transplanted to the paddy in a single plant hill spacing 30 cm ${\times}$ 25cm. Measurements of plant height were made from the juncture between culm and roots to the tip of the longest panicle of a plant pulled out from the paddy when they are matured. The results are summarized as follows: 1. Tall indica varieties originated from the south-east Asian countries could be classified into two groups depending upon their allelism whether they showed monogenic segregating ratio of 3:1 or not when they were crossed to T(N)1. 2. Most of U.S. varieties, especially semi-dwarf breedirg materials such as CP231 ${\times}$ SIO17 and B569A12, did not show monogenic segregating mode of 3:1 ratio when they were crossed to T(N)1 or to other varieties bearing the same genetic allele of T(N)1 such as Peta and Sigadis.

• Animal Bioscience
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• v.36 no.8
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• pp.1180-1189
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• 2023

Objective: Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed. Embryos in the early blastocyst stage possess two different cell populations, the inner cell mass (ICM) and trophectoderm. Then, cells in the ICM segregate into epiblasts (Epi) and primitive endoderm (PrE). PrE cells in embryos show specific expression of platelet-derived growth factor (PDGF) and its receptor, PDGF receptor A (PDGFRA). In this study, we suppressed PDGF signaling using two methods (CRISPR/Cas9 injection and inhibitor treatment) to provide insight into the segregation of embryonic lineages. Methods: CRISPR/Cas9 RNAs were injected into parthenogenetically activated and in vitro fertilized embryos. The PDGF receptor inhibitor AG1296 was treated at 0, 5, 10, and 20 µM concentration. The developmental competence of the embryos and the number of cells expressing marker proteins (SOX2 for ICM and SOX17 for PrE) were measured after the treatments. The expression levels of the marker genes with the inhibitor were examined during embryo development. Results: Microinjection targeting the PDGF receptor (PDGFR) A reduced the number of SOX17-positive cell populations in a subset of day 7 blastocysts (n = 9/12). However, microinjection accompanied diminution of Epi cells in the blastocyst. The PDGF receptor inhibitor AG1296 (5 µM) suppressed SOX17-positive cells without reducing SOX2-positive cells in both parthenogenetic activated and in vitro fertilized embryos. Within the transcriptional target of PDGF signaling, the inhibitor significantly upregulated the Txnip gene in embryos. Conclusion: We identified that PDGF signaling is important to sustain the PrE population in porcine blastocysts. Additionally, treatment with inhibitors was a better method to suppress PrE cells than CRISPR/Cas9 microinjection of anti-PDGF receptor α gene, because microinjection suppressed number of Epi cells. The PDGF receptor might control the number of PrE cells by repressing the proapoptotic gene Txnip. Our results can help to isolate Epi-specific cell lines from blastocysts.

• Journal of the Korean Geotechnical Society
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• v.24 no.1
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• pp.37-49
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• 2008

A geothermal heat pump system is a preferable alternative energy system in Korea because it uses the heat energy of the earth, which is environmentally friendly and inexhaustible. In order to characterize the thermal conductivity and viscosity of grout materials used for backfilling ground heat exchangers, nine bentonite grouts, one marine clay from Boryung, and cement grouts adapted in the United State have been considered in this study. The bentonite grouts indicate that the thermal conductivity and viscosity increase with the content of bentonite or filler (silica sand). In addition, material segregation can be observed when the viscosity of grout is relatively low. The marine clay turns out to be unsuitable for backfilling the ground heat exchanger due to its insufficient swelling potential. The saturated cement grouts appear to possess much higher thermal conductivity than the saturated bentonite grouts, and the reduction of thermal conductivity in the cement grouts after drying specimens is less than that in the case of the bentonite grouts. Maintaining the moisture content of grouts is a crucial factor in enhancing the efficiency of ground heat exchangers.

• Journal of the Korean Geotechnical Society
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• v.22 no.12
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• pp.5-14
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• 2006

In general, the dredged ground was composed of a big difference of sediment shape through segregating sedimentary of finer soil in case of reclaiming by dredged coarse soils. Therefore, this study was performed to evaluate the change of settling velocity of flow, and the density of sedimentary which is based on settling tests and self-weight consolidation tests, and consolidation test by seepage force according to the percentage of coarse of Kunsan dredge soils. The Yano's method has been applied to estimate the settlement of self-weight consolidation in finer soils at design but it only considers pouring water content and elevation of interface, therefore the other method needs to be introduced for the exact prediction of the settlement of coarse soil in which the segregation sedimentation is occurring. In this study, the settlement of self-weight consolidation was calculated by the change of the density of segregating sedimentary of coarse and finer soils which was analyzed by Yano's method to extend a serious of researches. The self-weight consolidation by Yano's method will not reflect the segregated settling in dredging coarse soil under 40% of #200 passing percentage. As a result, the evaluation technique of settlement of self-weight consolidation considering a change of the density of segregating sedimentary is suggested as a reasonable method that considers the sediment shape of coarse soil.

• The Korean Journal of Zoology
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• v.13 no.1
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• pp.21-25
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• 1970

The present paper deals with the results of the experiments, in which the resistibility to NaCl in whole develomental stages is examined by the emergence rate and the effects of NaCl on the SD action in Drosophila melanogaster. The four SD strains and one mutant strain(cn bw)are used and NaCl media are prepared y adding NaCl at a concentration of 1.0M, 0.7M, 0.5M, 0.3M, 0.1M, and 0.0M to the standard media for the present investigations. The results are given below. 1. The emergence rate (resistibility to NaCl) is not significantly different among strains but strikingly different among concentrations of NaCl. 2. The emergence rate decreases as the concentration of NaCl increases; the four SD strains are considerably resistible to the NaCl from a concentration of 0.0M to 0.3M but are susceptible from 0.5M or higher concentrations of NaCl. 3. No eggs are hatched from the culture media containing a concentration of 1.0M NaCl. This suggests that the SD strains are not resistible to NaCl at a concentrations of 1.0M or higher. 4. The difference in k values is not signficant among strains and also among concentrations of NaCl. Thus the SD action is not affected as far as once emerged from the culture media whether containing NaCl or not.

• Korean Journal of Social Welfare Studies
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• v.41 no.2
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• pp.309-334
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• 2010

This study explores gender mechanism of welfare politics in Korea from the perspective of welfare status theory. In the simplistic analysis, it is found that there is no gender difference in welfare attitudes. In the more sophisticated analysis of welfare-status-beased SEM, however, it is found that Korean welfare politics is to be understood in terms of gender-related politics. Three major findings of this study are as follows. Firstly, Korean women possess less pro-welfare attitudes in terms of the status of welfare beneficiary. This is due to the visible gender segregation in the Korean labor market on the one hand and due to the centrality of social insurance programs in the structure of Korean public welfare system on the other hand. Secondly, Korean women have relatively higher level of pro-welfare attitude when it comes to the status of welfare service provider. Thirdly, Korean women tend to be more supportive to pro-welfare parties as they have less chance to be a higher taxpayer. Based on the above mentioned findings, this study concludes that it is possible to make Korean women be more pro-welfare if the Korean welfare state expands more social services that tend to meet urgent needs of women.

• Korean Journal of Culture and Social Issue
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• v.17 no.2
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• pp.219-239
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• 2011

The objective of this study is to investigate the influence of husbands' sex role stereotype, intercultural sensitivity, and the acceptance of wife's culture on immigrant women's acculturation, marital satisfaction and life satisfaction in multi-cultural family in Korea. The data of 100 immigrant women and their husbands were analyzed. Husbands' mean age was 41.6 and wifes' mean age was 29.6. The results of this study are as follows. The first, husbands' sex role stereotype, intercultural sensitivity and the acceptance of wives' culture together explain only marginalization among wives' acculturation(integration, assimilation, segregation, and marginalization) significantly, but individual variable's effect was not significant. The second, husbands' acceptance of wives' culture explain marital and life satisfaction significantly. Third, only marginalization among four types of acculturation explain significantly marital and life satisfaction significantly. integration explain only life satisfaction significantly. Husbands' sex role stereotype is related with the acceptance of their wives' culture and their intercultural sensitivity negatively. In wives' acculturation, there is low positive correlation between assimilation and integration and moderate negative correlation between assimilation and marginalization. This study revealed that the marital and life satisfaction of immigrant women in intercultural family in Korea are explained by acculturation which was influenced by their husband's acceptance of wives' culture and they are not only immigrant wife's problem but multicultural problem.

• Journal of the Korean Association of Geographic Information Studies
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• v.26 no.4
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• pp.251-265
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• 2023

The purpose of this study is to deduce key indicators in evaluating the pedestrian environment for the elderly in Busan, which has entered an aging society, and to propose policy improvement measures. The key indicators were selected based on prior research and surveys, and the effectiveness of those indicators were measured through evaluations conducted on three places which have the highest proportion of the elderly population in Busan. The summaries of analysis are as follow. First, the three places are hillslide residential areas, and areas of activity that the elderly have were very narrow due to the restrictions on their movement caused by slope. Second, the areas were filled with a number of illegally parked vehicles. And the degree of segregation of pedestrian and vehicle and the level of safety were very low. Third, the streets with steep slopes of the target site force the elderly to move vertically, and for this reason, the elderly are expressing difficulties in outdoor activities. Fourth, it was found that the target site lacked a space for relaxation during outdoor activities. The poor walking environment not only limit the essential and social activities of the elderly, but also adversely affects the health and quality of life of the elderly. In order to maintain the health of the elderly and improve the quality of life, actions should be taken to improve the walking factors that affect the movement and external activities of the elderly.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.154-155
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• 2012

The promise of nano-crystalites (nc) as a technological material, for applications including display backplane, and solar cells, may ultimately depend on tailoring their behavior through doping and crystallinity. Impurities can strongly modify electronic and optical properties of bulk and nc semiconductors. Highly doped dopant also effect structural properties (both grain size, crystal fraction) of nc-Si thin film. As discussed in several literatures, P atoms or radicals have the tendency to reside on the surface of nc. The P-radical segregation on the nano-grain surfaces that called self-purification may reduce the possibility of new nucleation because of the five-coordination of P. In addition, the P doping levels of ${\sim}2{\times}10^{21}\;at/cm^3$ is the solubility limitation of P in Si; the solubility of nc thin film should be smaller. Therefore, the non-activated P tends to segregate on the grain boundaries and the surface of nc. These mechanisms could prevent new nucleation on the existing grain surface. Therefore, most researches shown that highly doped nc-thin film by using conventional PECVD deposition system tended to have low crystallinity, where the formation energy of nucleation should be higher than the nc surface in the intrinsic materials. If the deposition technology that can make highly doped and simultaneously highly crystallized nc at low temperature, it can lead processes of next generation flexible devices. Recently, we are developing a novel CVD technology with a neutral particle beam (NPB) source, named as neutral beam assisted CVD (NBaCVD), which controls the energy of incident neutral particles in the range of 1~300eV in order to enhance the atomic activation and crystalline of thin films at low temperatures. During the formation of the nc-/pm-Si thin films by the NBaCVD with various process conditions, NPB energy directly controlled by the reflector bias and effectively increased crystal fraction (~80%) by uniformly distributed nc grains with 3~10 nm size. In the case of phosphorous doped Si thin films, the doping efficiency also increased as increasing the reflector bias (i.e. increasing NPB energy). At 330V of reflector bias, activation energy of the doped nc-Si thin film reduced as low as 0.001 eV. This means dopants are fully occupied as substitutional site, even though the Si thin film has nano-sized grain structure. And activated dopant concentration is recorded as high as up to 1020 #/$cm^3$ at very low process temperature (＜ $80^{\circ}C$) process without any post annealing. Theoretical solubility for the higher dopant concentration in Si thin film for order of 1020 #/$cm^3$ can be done only high temperature process or post annealing over $650^{\circ}C$. In general, as decreasing the grain size, the dopant binding energy increases as ratio of 1 of diameter of grain and the dopant hardly be activated. The highly doped nc-Si thin film by low-temperature NBaCVD process had smaller average grain size under 10 nm (measured by GIWAXS, GISAXS and TEM analysis), but achieved very higher activation of phosphorous dopant; NB energy sufficiently transports its energy to doping and crystallization even though without supplying additional thermal energy. TEM image shows that incubation layer does not formed between nc-Si film and SiO2 under later and highly crystallized nc-Si film is constructed with uniformly distributed nano-grains in polymorphous tissues. The nucleation should be start at the first layer on the SiO2 later, but it hardly growth to be cone-shaped micro-size grains. The nc-grain evenly embedded pm-Si thin film can be formatted by competition of the nucleation and the crystal growing, which depend on the NPB energies. In the evaluation of the light soaking degradation of photoconductivity, while conventional intrinsic and n-type doped a-Si thin films appeared typical degradation of photoconductivity, all of the nc-Si thin films processed by the NBaCVD show only a few % of degradation of it. From FTIR and RAMAN spectra, the energetic hydrogen NB atoms passivate nano-grain boundaries during the NBaCVD process because of the high diffusivity and chemical potential of hydrogen atoms.