• Journal of Korean Society of Forest Science
• /
• v.109 no.1
• /
• pp.41-49
• /
• 2020

In this study, the effect of seed pre-treatments and pellet coating materials to enhance the efficiency of large-scale propagation of Aster koraiensis seeds were investigated. Seeds were immersed in water for one day, and only those that sank were used for pre-treatment to use filled seeds. Pre-treatments were divided into hormone treatments, with gibberellic acid (GA₃; 200 and 500 ppm) and 24-epibrassinolide (10^-6, 10^-7, and 10^-8M), and priming with potassium nitrate (100 mM of KNO₃). To produce pellet-coated seeds, pellet materials (DTCS or DTK) were applied to control (unprimed) and primed seeds with binders (PVA or CMC). The maximum germination percent (GP) of seeds before pellet coating was 65% (with the priming treatment), and there was no difference in the GP of seeds among hormone treatments. For seeds sown in a growth chamber on filter paper, GP was 41% for control (unprimed/uncoated) seeds, 65% for uncoated primed seeds, 71% for DTCS/PVA-pellet-coated seeds, and 42% for DTK/CMC-pellet-coated seeds. Seeds that were primed first and then pellet-coated showed greatly improved GP, mean germination time (MGT), and germination rate than seeds that were only pellet-coated. For seeds sown in commercial soil in a greenhouse, control seeds had a GP of 27%, whereas primed seeds had the highest GP (58%), and their MGT and GT were 9.4 days and 7.0%·day, respectively. In addition, DTK/PVA-pellet-coated seeds (40%) also had a GP higher than the control (27%), and their MGT was 15-27 days. For seeds sown in sandy-loam soil in a greenhouse, unprimed-pellet-coated seeds and primed-pellet-coated seeds both had GPs ranged of 39%, which were lower than that of control seeds. In general, the seeds that were pellet-coated with DTK had a higher GP than those pellet-coated with DTCS. Furthermore, the MGT of unprimed-pellet-coated seeds was 15.0-19.8 days, which was longer than the MGT of primed-pellet-coated seeds. These results suggest that priming enhances seedling emergence of Aster koraiensis seeds. Moreover, when priming is combined with pellet coating, DTK is a more suitable pellet material than DTCS, and PVA and CMC are equally suitable adhesives.

• Journal of the Korean Society of Tobacco Science
• /
• v.4 no.2
• /
• pp.37-39
• /
• 1982

This experiment was conducted to obtained basical source for gathering seeds in flue- cured (NC 2326), and to investigate some characters on the relation to gathering number of capsules per a plant; amount of gathering seeds, weight per 1000 seeds and germination rate, respectively. As a results; correlation coefficient of number of capsules and amount of gathering seeds was pros(live correlation (r =0.9771**) , Weight per 1000 seeds (0.16g) was good from 39$\pm$2 capsules and amount of gathering seeds was 4.8g. So it is considered that method of gathering seeds wise proved to be a desirable 39: 2 capsules and amount of gathering seeds was 4- 5 gram.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.62 no.1
• /
• pp.9-15
• /
• 2017

Germination characteristics, seedling emergence, and early seedling growth of iron-coated rice seeds, cultivars Daebo and Samdeokbyeo, under different water depths were compared with those of non-coated seeds (control) and the results evaluated to obtain basic information for establishing stable seedlings in direct water seeding. The total germination percentage of the two seed treatments was similar, but iron-coated seeds had slightly faster germination and shorter mean germination time than non-coated seeds. Water absorption rates of iron-coated seeds were lower than that of non-coated seeds during seed germination. The germination percentage of the two iron-coated rice seed cultivars showed a significant decline of 15-22% after one year of storage under natural conditions. The seedling emergence percentage and uniformity of the two rice cultivars were significantly higher in the iron-coated seeds at 1-13 cm water depths but the percentage of floating seedlings was lower in iron-coated seeds than in non-coated seeds. The iron-coated seeds had a high seedling emergence percentage of 91.3-93.3% at all flooding depths whereas the non-coated seeds had a significantly low seedling emergence percentage of 57.7-71.7% at a water depth of 13 cm. Moreover, the shoot dry weight and seedling health score of iron-coated seeds were significantly higher than those of non-coated seeds, while root dry weights were similar in iron-coated and non-coated seeds, regardless of water depth. These results suggest that iron-coated seeds are more appropriate for stable seedling establishment in direct water seeding than are non-coated seeds.

• Journal of Environmental Science International
• /
• v.30 no.1
• /
• pp.77-85
• /
• 2021

This study was carried out to establish optimal conditions for breaking dormancy of Agastache rugosa O. Kuntze seeds. A series of experiments according to seed maturity and treatment with plant growth regulators were performed to improve germination percentage and synchronize germination of the seeds. In addition, it was conducted to test whether the useful effect of seed treatment before sowing leads to healthy seedling and early vigorous growth. The average seed size was 1.85 mm (length) × 0.82 mm(width). The seed size was much smaller than other vegetable seeds. Seeds colorappeared dark brown, the shape of the seeds was oval and the weight of 1,000seeds was 352.8 mg. The optimum germination temperature was 22℃. Light exposure during germination did not affect germination promotion, suggesting that A. rugosa seeds are a kind of dark germinating seeds. Seed dormancy lasted for 40 days after harvesting, and GA3 treatment of dormant seeds could break dormancy. There were significant differences in germination percentage and rate according to the maturity of seeds. The germination percentage of mature seeds was 10 - 18% higher than that of immature seeds, and germination rate was 2 days faster. GA3 treatment during growth regulator treatment improved germinability, but BAP or ethephone treatment did not. The optimal growth regulator concentration of for germination was the combination treatment of 100 mM GA3 ＋ 100 mM BAP.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.18 no.1
• /
• pp.93-100
• /
• 2016

This research was conducted to determine a pre-germinated herbicides with iron-coated seeds in water and wet hill seeded rice. Days of rice seedling emergence was the faster germination at the application of benzobicyclon > oxadiazon > thiobencarb > butachlor and the sealed iron-coated seeds with pre-germinated seeds> pre-germinated seeds> iron-coated seeds with pre-soaking. The seedling establishment was relatively high in untreated control and benzobicyclon among seed treatments by 63.7% and 75.7%. There was 100% seeds killed of pre-germinated seeds in terms of herbicide phytotoxicity and sealed iron-coated seeds with pre-germinated seeds in butachlor but benzobicyclon was of safe with 2% rice seeds killed. Infant rice seedling height was of 9.2-12.9cm in benzobicyclon and 11.9-16.3cm in untreated control and thus there was relatively normal development and growth at the initial rice seedling.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.45 no.2
• /
• pp.108-111
• /
• 2000

Osmoconditioning and hardening effects for the seed germination of normal and naturally aged rice seeds were studied through analyzing the total sugars and $\alpha$-amylase activity. The normal seeds which used to have high germination rate accelerated germination with the osmoconditioning at the suboptimal temperature of 17$^{\circ}C$. On the other hand, the aged seeds did not affect germination rate at $25^{\circ}C$, while increased germination rate and accelerated germination a little at 17$^{\circ}C$, Hardening of aged seeds increased germination rate by 10-15% compared with control seeds at both 17 and $25^{\circ}C$ and accelerated germination. Total sugar content and $\alpha$-amylase activity of normal seeds were higher than did aged seeds. The aged seeds with treatment of osmoconditioning and hardening increased total sugar content and $\alpha$--amylase activity, but hardening was more effective than osmoconditioning. The $\alpha$--amylase activity was positively correlated with the total sugar content and germination rate.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.54 no.4
• /
• pp.346-350
• /
• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.4
• /
• pp.407-412
• /
• 1995

Experiments were conducted to obtain information on separation of nonviable seeds from seed lots by the nondestructive ways. Seeds of sesame, welsh onion and lettuce were artificially aged at 90% relative humidity and 45$^{\circ}C$ to get different seed qualities. The relationships between seed quality and leakage of total sugars, amino acids, and proteins into soaking water were determined to know a possibility of grading seeds. Dead seeds of lettuce leaked significant amounts of total sugars, amino acids, and proteins, while high quality seeds leaked negligeable amounts of total sugars and some of amino acids and proteins. Dead seeds of welsh onion leaked significant amounts of amino acids and some total sugars and proteins, while high quality seeds leaked negligeable amounts of these compounds. Sesame seeds leaked little total sugars, amino acids, and proteins regardless of seed quality.

• Journal of Plant Biotechnology
• /
• v.40 no.1
• /
• pp.43-48
• /
• 2013

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

• Journal of Forest and Environmental Science
• /
• v.37 no.3
• /
• pp.235-242
• /
• 2021

Seed morphology, physiology and environmental conditions have influence on germination of any tropical seeds and their appropriate handling, processing and handling enhances seeds emergent. This study therefore investigated effects of storage durations under different temperatures on germination of Bombax costatum seeds from different provenances. Fresh 25 seeds of B. costatum in four replicates were collected from four different provenances (Aponmu, Oluwa, Ibadan and Oyo). They were surface sterilized, thoroughly washed in distilled water and stored at four different temperature regimes: 28℃, 21℃, 5℃ and -17℃. Samples were taken every 2 months for germination test for 18 months. Germination assessment was carried out daily and recorded. The experimental design was 4×4×10 factorial in 4 replicates. Data were subjected to percentages and analysis of variance (ANOVA). Results showed that there were significant differences among storage temperatures, storage durations and provenances on germination percentage. Freshly collected seeds from Aponmu and Oyo had the highest mean germination of 100% followed by seeds from Ibadan (89.3%). Seeds from Oyo provenance stored 5℃ had germination of 94.0%, 88.70% and 78.7% at 2nd, 4th and 6th month respectively. Seeds from Ibadan and Oyo stored at 28℃, 21℃ recorded 0.0% starting from 8 months of storage. Germination of B. costatum seeds from the four provenances decreased with increase in storage duration at different temperatures.