• The Korean Journal of Ecology
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• v.12 no.1
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• pp.21-35
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• 1989

It was found that seed germination and seedling growth of selected species were inhibited by phytotoxic substance released from black pine. The longer was the aqueous extraction time of black pine needles, the smaller was the relative germination and growth of the species were inhibited in aqueous leachate and rain leachate as well as in soil underneath the black pine trees. In addition, the growth of lettuce was suppressed in volatile substances from black pine needles. To detect phytotoxic substances of black pine needles, paper chromatography and high performance liquid chromatography were used and fourteen kinds of KDICical compounds were isolated and identified as phenolic acids including benzoic acids. The growth of lettuce was inhibited in the experiment using reagents identical to these KDICical substances and a great inhibition was observed in the concentration of less than 5$\times$ M.

• The Korean Journal of Ecology
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• v.21 no.2
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• pp.133-137
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• 1998

Well grown trees of ficus bengalensis produce one or more potential inhibitors of seed germination and seedling growth. The aqueous extract of ficus leaf and bark enhanced the shoot length aqueous leaf extract of F. bengalensis. Bark extract of F. bengalensis inhibited the shoot length and root length of the plant at high concentration. Both the bark and leaf extract inhibited the seed germination. The postemergence and preemergence treatment of bark and leaf extract of F. bengalensis reduced the shoot biomass. The result suggest that F. bengalensis may have potential allelochemicals which may be developed as natural herbicides.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.372-380
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• 2014

BACKGROUND: This study was carried out in order to closely examine the influence of fertilization upon growth in container of seedling in indeciduous Quercus species (Q. mysinaefolia, Q. acuta and Q. glauca). METHODS AND RESULTS: Fertilizer level was made by adjusting water soluble compound fertilizer (N:P:K=19: 19:19, v/v) to 1000, 2000, $3000mg{\cdot}L^{-1}$ level along with non-fertilizing plot. Fertilization increased height, root collar diameter growth, and dry weight in these three species of trees. The more increase in fertilizer level led to the more rise even in growth of these species. H/D ratio and T/R ratio also showed tendency of getting bigger in the more rise in fertilizer level. Photosynthetic rate was shown to get higher in the higher fertilizer level according to fertilization in all the three species. In the analysis of root morphological traits, the total root length was surveyed to be longer in the more rise in fertilizer concentration. As even a case of root project area, surface area, and root volume is the similar tendency to characteristics in the total root length, a rise depending on fertilization was observed. CONCLUSION: In light of the results in this experiment, the fertilizer level is judged to be $2000mg{\cdot}L^{-1}$ level that is proper for production of 1-year-old container seedling in indeciduous Quercus species with excellent root development and high seedling quality index.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.266-273
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• 2008

The effects of soil acidification on the seedling growth and nutrition of Pinus densiflora and Quercus acutissima were investigated. The relationship between the seedling growth and molar (Ca+Mg+K)/Al ratio of in soil solution was examined. The results suggested that growth inhibition of seedling Pinus densiflora and Quercus acutissima was due to the low pH of soil solution, which was followed by leach of Al into soil solution, and decrease of essential elements, such as Ca in aerial pant of the seeding caused by the increase of Al concentration in subterranean pant of the seedlings. The level of growth inhibition was determined not only by Al concentration, but also by the balance of inorganic elements, Al, Ca, Mg and K. The growths of two species in total dry weight were clearly inhibited when molar (Ca+Mg+K)/Al ratio of the soil was lower than 6.0. The growth in dry weight, in the condition of the molar ratio was 0.8, was decreased 60% or 50% for the seedling of Pinus densiflora or Quercus acutissima respectively. It was concluded that the molar (Ca+Mg+K)/ Al ratio could be an important index for evaluation of the effects of soil acidification, due to acid deposition such as acid rain, on growth of trees and nutrition. And it might be a more useful indicator for evaluation of critical load of acid deposition on forest ecosystems.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.4
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• pp.276-280
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• 2013

D. kaki and D. lotus are used as rootstocks for astringent persimmons in Korea but characteristics of their seedlings have not been determined. In this experiment, their seeds were sown in 3-L pots on April 18 and the seedlings were grown until October 24. Growth and nutrient absorption were compared at the end of the season after destructively harvesting the seedlings. Seedling growth of D. lotus was much faster than that of D. kaki in terms of total stem length, stem diameter, and number of leaves. However, chlorophyll value and specific leaf weight were higher in D. kaki than in D. lotus. Dry weight of D. lotus was 3.6- and 3.7-fold higher than that of D. kaki in above-ground parts and the root, respectively. D. kaki seedlings were characterized by higher concentrations of N, P, K, Ca, and Mg in the leaves, stem, or the root. However, total contents of the elements were 1.8- to 3.7-fold higher in a D. lotus seedling due to its greater dry weight. Since D. lotus seedlings absorbed more inorganic elements on a tree basis and grew more vigorously than D. kaki seedlings, the level of fertilization for astringent persimmons should be adjusted depending on rootstocks to maintain the trees at the optimum vigor.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.54-61
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• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• Journal of Korean Society of Forest Science
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• v.106 no.1
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• pp.10-18
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• 2017

This study was carried out to suitable plantation site from comparison and analysis of regional early growth characteristics of planted Larix kaempferi trees in harvested L. kaempferi plantations. Two-year-old bare-root seedlings of L. kaempferi were planted with the density of $3000\;seedlings\;ha^{-1}$ at four sites (Yeongju, Gimcheon, Chuncheon, and Inje). All sites were established with three plots ($400m^2$ per plot) in 2010. We analyzed soil physical and chemical properties in October 2010 and 2013. We measured root collar diameter (RCD) and height of trees in October from 2010 to 2014, and then calculated H/D ratio and stem volume. Soil pH was significantly increased and available P was significantly decreased 3 years after planting. Annual mean RCD, height, and stem volume of 5-to 7-year-old in the Chuncheon site were significantly higher than those in the other sites, however them in the Yeongju site were the lowest. It is related to the fact that total nitrogen, available phosphorus, and organic matter concentrations in the Yeongju site were relatively lower than those in the other sites. As a results of this study, we could confirm the correlation between soil properties of L. kaempferi plantations and growth of L. kaempferi trees. We could suggest values of soil properties for reforestation of L. kaempferi.

• Korean Journal of Agricultural Science
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• v.43 no.1
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• pp.21-27
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• 2016

Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these 'S' isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.394-404
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• 2022

This study was conducted to optimize the amount of drip irrigation for Prunus×yedoensis Matsum., one of the major medium-sized landscaping trees used mainly for streetscapes and as ornamentals. The experiment was conducted in a container nursery, where we assessed the growth and physiological characteristics of 4- and 8-year-old seedlings watered at different rates (x) by a drip irrigation system. The relative growth rates (based on height and root collar diameter (RCD) measurements) were highest at 288 and 416 L/year/tree for the 4- and 8-year-old containerized seedlings, respectively. These age and treatment combinations also produced significantly different dry weight and seedling quality index values, indicating good growth. The two age groups had significantly different total root lengths, root diameters, and root volumes under these respective irrigation treatments. In addition, the 4-year-old containerized seedlings irrigated at 288 L/year/tree and the 8-year-old containerized seedlings irrigated at 416 L/year/tree had the highest activations in their chlorophyll contents. Overall, the results (differences in irrigation amounts affect the seedlings morphological relative growth, biomass growth, seedling quality, and physiological reaction) indicate that the optimal irrigation amounts for container-grown Prunus×yedoensis are 288 L/year/tree for 4-year-old (RCD class, 3cm) and 416 L/year/tree for 8-year-old (RCD class, 7 cm) containerized seedlings.