• Korean Journal of Microbiology
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• v.43 no.4
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• pp.279-284
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• 2007

Two bacterial strains isolated from soil (Bacillus subtilis strains: PS2 and RFO41) were evaluated to determine their promoting effect on the growth of tomato seedling under axonic and pot conditions. The production of phytohormone, such as indole-3-acetic acid, indole-3-butyric acid, gibberellin and zeatin by these two strains was investigated as possible mechanisms for plant growth stimulation. Both PS2 and RFO41 were shown to produce various phytohormones, and. the production of phytohormones was stimulated by the addition of peptone-rich brain heart broth medium. In addition, these bacteria exhibited high levels of phosphatase activity, which ranged from 2.18 to $2.7\;{\mu}\;{\rho}-nitrophenol/ml/hr$. PS2 and RFO41 were applied to the pot test for growth of tomato seed with phosphate. Root and shoot lengths of germinated tomato after 15 days were 45.5% and 36.5% longer than that of control in RFO41 treated samples, respectively. Baciller sp. PS2 and RFO41 may have a potential for biofertilizer in the agriculture.

• The Korean Journal of Ecology
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• v.25 no.1
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• pp.1-7
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• 2002

The ecological background of a restoration project is complex and difficult to betermine without experimentation. A useful context for experiments is the well-studied process of natural succession, because the factors that drive or inhibit succession are also at work during reclamation (a form of primary succession) and restoration (which often resembles secondary succession). Using experimental studies on urban wasteland reclamation, we have tested for factors that stimulate or inhibit succession during early phases of woodland development in the Northeastern United states. The emphasis has been on mutualisms (seed dispersal, pollination, and mycorrhizae) and microsite limitations in the recruitment, growth, and reproduction of woody plants. Using plantings of seeds, seedlings, and clusters of reproductively mature plants on abandoned landfills, we have observed that (1) soil microsite deficiencies lead to very poor germination (<0.1$\%$) and seedling survival (<0.01$\%$) of most native species; (2) seed dispersal by birds is a significant and reliable source of woody plant recruitment; however (3) proximity effects are strong, with most (up to 95$\%$) of seed rain falling in the vicinity of planted clusters that are closest to putative seed sources; and (4) remnant natural woodlands are critical components of the recruitment process. To emphasize the last point, in one case, we found that the destruction of approximately 50$\%$ of nearby natural woodland vegetation led to a commensurate decline in seed rain. In another case, we found that the species richness of recruits was strictly limited by the species composition of nearby source plant communities, with no evidence of community enrichment by long distance dispersal over 5 years. We conclude from these results that the size and proximity of remnant natural populations are critical considerations when planning reclamation and restoration programs that rely on natural successional processes.

• Journal of Korean Society of Forest Science
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• v.67 no.1
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• pp.10-16
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• 1984

Containerized Quercus acutissima seedlings were inoculated with vegetative mycelial inoculum of Pisolithus tinctorius and were grown in a green-house. The ectomycorrhizal fungus, P. tinctorius (Pt) increased growth of the container-grown oak seedlings. Of three treatments, Pt inoculation stimulated remarkably primary lateral roots, shoot growth, and root collar diameter and leaf area development, compared with either non-inoculated or natural soil treatment. In addition, Pt-inoculated seedlings produced ectomycorrhizae, by 71.1 percent. However, noninoculated and naturally grown seedlings were free of natural ectomycorrhizal. Ectomycorthizal roots developed more abundantly in the middle portion of the container than in the top or bottom portions.

• Korean Journal of Agricultural Science
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• v.10 no.2
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• pp.263-268
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• 1983

In a survey of seed-borne mycofloras of Glycine max, 21 species have been isolated. Alternaria tenuis, Aspergillus spp., Cercospora kikuchii, Penicillium spp. and Fusarium spp. were the predominant fungi. C. kikuchii was isolated more often from purple stained seed than from unstained seed, and Fusarium spp., Phomopsis sp., Aspergillus spp. and Penicillium spp. were isolated less frequently in stained than unstained seeds. In inoculation experiments, C. kikuchii, Colletotrichum dematitum, Phomopsis sp. produced mild to severe symptoms on leaves when suspension of their conidia and mycelia were sprayed on to plants. In soil inoculation experiments, Fusarium oxysporum, F. moniliforme and F. semitectum showed pathogenicity causing seed rot and seedling blight.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.266-266
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• 2017

In recent years, adoption of the System of Rice Intensification (SRI) is spreading in most Asian countries, and more recently in Cambodia is one of the Asian countries with high adoptions of SRI. We conducted on-farm experiment to investigate the effects of SRI on rice growth and yield in the farmers' fields in rainfed region of southern Cambodia. The study was undertaken in rainfed lowland fields of Popel commune ($11^{\circ}$ 04' 67" N, $104^{\circ}$ 40' 79" E) of Tram Kak District in Takeo Province during the wet seasons in 2012, 2013, and 2015. A total of 32 on-farm experiments were conducted during the wet seasons for three years of 2012 (11 fields), 2013 (8 fields), and 2015 (13 fields). Across the three years of study, SRI produced significantly greater plant biomass and grain yield than Non-SRI. The yield increase was mostly ascribed for the increased number of grains per land area, which was due to the increased number of spikelets per panicle rather than the number of panicles per land area. With no significant difference between SRI and Non-SRI with respect to seedling age, the greater number of grains per panicle was accounted for by the reduced planting density and increased amount of manure application in SRI than Non-SRI fields. It was found that the greater manure application has increased soil nitrogen content in SRI and Non-SRI fields. While SRI did not increase the number of panicles per land area, it did increase the number of panicles per hill.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.182-182
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• 2017

The objective of this research was to determine controlling effects on rice blast (Pyricularia oryzae) in rice plants by using plant extracts from different extraction methods (water, boiling water, fermentation, and ethanol) from 38 agricultural materials of traditional agriculture. Rice blast was completely suppressed by 3% ethanol extracts in Rheum palmatum roots, and suppressed 97% and 77% by 10% ethanol extracts in onion bulb and pine tree leaves, respectively in a laboratory test. However, other agricultural materials showed low effect on suppression of rice blast. Additionally, in a seedling test, rice injury of two cultivars (Ilmibyeo and Hopyoungbyeo) against rice blast was reduced 40-71%, 29-63%, and 23-63% by 5 and 10% ethanol extracts in Rheum palmatum roots, onion bulb, and pine tree leaves, respectively, compared with non-treated controls. Rice injury of two cultivars (Ilmibyeo and Hopyoungbyeo) against rice blast was reduced by 21-55%, 23-46%, and 5-39% in response to Rheum palmatum roots, onion bulb, and pine tree leaf applications at 100, 200 and $400g/m^2$ at 0 day after seeding, respectively, compared with non-treated controls. Rice plants did not show any leaf injuries and growth reduction after treatments of the Rheum palmatum roots, onion bulb, and pine tree leaf extracts or soil application. Thus, the above materials may be used for controlling rice blast in organically produced rice fields.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.1-6
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• 1994

Cotyledonary and hypocotyl explants of melon seedlings were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and benzyladenine (B.A).Up to 22% of cotyledonary explants and 7%, of hypocotyl explants, respectively: Produced somatic embryos through intervening two types of calli: bright yellow compact (BYC) callus and pale-yellow compact (PYC) callus. BYC callus was capable of producing somatic embryos at initial culture, but it became necrotic as subrulhues proceeded. In contrast UC callus was incapable of producing somatic embryos during initial culture (first 6 weeks), but it became bright-yellow friable (BYF) callus with forming a few globular embryos after 2 months of subculture, indicating that the callus turned embryogenic. The embryogenic capacity of BYF maintained for over one year when the callus was sucultured at 4-week interval. Upon transfer onto MS basal medium the callus gave rise to numerous somatic embryos and subsequently converted to plantlets. Plantlets were transplanted to potting soil and grown to maturity in the phyotron.

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.95-100
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• 2001

To select the section with shoot formation ability, the calli and shoot formation from three sections (first leaf including cotyledonary node, hypocotyl and cotyledon explants) of 5-days-seedlings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section (10%) and the cotyledon section (0%). The shoot formation rates and shoot number of the four excised sections (whole first leaf, a half of the first leaf, a third of the first leaf and only node) of the first leaf were also investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and numbers (3-4 / explants) than those in other two explants. Effects of six cytokinins (kinetin, zeatin, BAP, 2iP, PBA, and TDZ) on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0 mg/L) exhibited the highest in shoot formation rate (94%) and numbers (8 / explant). In addition, the combined effects of three cytokinins (zeatin, BAP, and TDZ; 0.5, 1.0, 2.0 mg/L, respectively) and an auxin (IAA; 0.0, 0.5, 1.0, 2.0 mg/L) were determined. The combination (1:1, v/v) of zeatin (1.0 mg/L) and IAA (1.0 mg/L) exhibited the highest in shoot formation rate (96%) and numbers (16 / explant), twice more than zeatin (1.0 mg/L) alone. The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at various concentrations. The highest root formation (8 / shoot) was achieved on the medium supplemented with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.

• The Plant Pathology Journal
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• v.20 no.1
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• pp.63-66
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• 2004

Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. However, yield losses reached up to 30-60％ due to various diseases during 3 or 5 years of ginseng cultivation in the country. Therefore, successful production of ginseng roots depends primarily on the control of diseases. The objective of this study was to select potential biocontrol agents from rhizobacteria isolated from various plant internal root tissues for the control of multiple ginseng diseases as an alternative to fungicides. Among 106 Bacillus strains, two promising biocontrol agents, Bacillus pumilus strain B1141 and Paenibacillus lentimobus strain B1146, were selected by screening against root rot of ginseng caused by Cylindrocarpon destructans in a greenhouse. Pre-inoculation of selected isolates to seed or l-year-old root of ginseng resulted in stimulation of shoot and/or root growth of seedlings, and successfully controlled root rot caused by C. destructans (P<0.05). Furthermore, drenching of cell suspension of the selected isolates on seedling-growing pots reduced the incidence of Phytophthora blight after the seedlings were challenged with zoospores of Phytophthora cactorum (P<0.05). P. lentimorbus strain B1146 showed antifungal activity against various soil-borne pathogens in vitro, while B. pumilus strain B1141 did not show any. Results of this study suggest that some rhizobacteria can induce resistance against various plant diseases on ginseng.

• Journal of Biosystems Engineering
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• v.30 no.2 s.109
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• pp.81-88
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• 2005

Manual transplanting Chinese cabbage needs 184 hours per ha in Korea. Mechanization of Chinese cabbage transplanting operation has been highly required because it needs highly intensive labor during peak season. This study was conducted to developed walking-type Chinese cabbage transplanter. In order to find out design factor of the transplanter, a kinematic analysis software, RecurDyn, was used. The prototype was tested in the circular soil bin and its operating motion was captured and analyzed using high speed camera system. Prototype was one row type which utilized original parts of engine, transmission and etc. from walking-type rice transplanter in order to save the manufacturing cost. Success ratio of pick-up device of hole-pin type and latch type were $96.0\%$ and $99.2\%$, respectively. which was highly affected by feeding accuracy of feeding device of seedling. Transplanting device of the prototype produced a elliptic loci which were coincident with those produced by the computer simulation. Prototype proved good performance in transplanting with mulching and without mulching operation, either. Working performance of prototype was 22 hours per ha and operation cost of the prototype was 961,757 won per ha. So, it would reduce $88\%$ of the labor and $29\%$ of operation cost.