• Title/Summary/Keyword: seedling soil

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Establishment of Acclimatization System and Growth Characteristics for Regenerated Plants of Oplopanax elatus Nakai (땃두릅나무 재분화 유식물체의 순화 체계 및 생육 특성)

  • Seong, Eun Soo;Yoo, Ji Hye;Kim, Hee Young;Choi, Hye Lim;Seo, Ji Won;Hwang, Myeong Ha;Kim, Myong Jo;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.6
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    • pp.397-403
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    • 2019
  • Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 mm, 10 mm - 30 mm and above 30 mm) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 mm showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.

Seed Germination and Seedling Survival Rate of Pinus densiflora and Abies holophylla in Open-field Experimental Warming Using the Infrared Lamp (적외선등을 이용한 실외 실험적 온난화 처리에 따른 소나무와 전나무의 종자 발아 및 유묘 생존율)

  • Cho, Min Seok;Hwang, Jaehong;Yang, A-Ram;Han, Saerom;Son, Yowhan
    • Journal of Korean Society of Forest Science
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    • v.103 no.2
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    • pp.203-210
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    • 2014
  • The purpose of this study was to investigate the effect of experimental warming using infrared lamps on seed germination and seedling survival rate of Pinus densiflora and Abies holophylla. The air temperature of warmed plots had been automatically maintained 3 higher than control plots. The percent germinations (%) of the two coniferous species were higher in warmed plots than in control plots, however a significant difference appeared only in A. holophylla. In addition, P. densiflora and A. holophylla showed the shorter mean germination time (days), higher germination rate ($seed{\cdot}day^{-1}$) and germination energy (%) in warmed plots than in control plots. A. holophylla showed a higher seedling mortality rate in the warmed plots than in control plots because of increased air and soil temperatures and decreased soil moisture. However, seedling survival rate of P. densiflora showed no significant difference by experimental warming. In the future, changed air and soil temperatures and soil moisture due to global warming will induce a variety of changes in seed germination and survival rate of tree species in nursery culture. Therefore, it is necessary to establish adaptation strategies that improve techniques in nursery culture against global warming.

Effect of Irrigation of Sulfur Solution before Sowing on Growth and Root Rot Disease of Seedling in Ginseng Nursery (파종전 무기유황 관주처리가 묘삼의 생육 및 뿌리썩음병 발생에 미치는 영향)

  • Lee, Sung Woo;Lee, Seung Ho;Park, Kyung Hoon;Jang, In Bok;Jin, Mei Lan;Kim, Ki Hong
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.5
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    • pp.391-397
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    • 2014
  • To control the disease of root rot in ginseng nursery, inorganic sulfur solution of 0.1%, 1.0%, and 2.0% were irrigated by amount of $10{\ell}$ per $3.3m^2$ before sowing. On the last ten days of July, Fusarium solani and F. oxysporum were similarly detected by 44.8% and 43.8%, respectively, while Cylindrocarpon destructans was low detected by 4.4% in the diseased seedling. The more sulfur's concentration was increased, the more soil pH was decreased. Soil pH was decreased from 5.87 to 4.59 by the irrigation of sulfur solution of 1.0%. The more sulfur's concentration was increased, the more electrical conductivity (EC) of soil was increased. EC was increased from 0.27 dS/m to 1.28 dS/m by the irrigation of sulfur solution of 1.0%. Irrigation of sulfur solution was effective on the inhibition of damping-off caused by Rhizoctonia solani in ginseng seedling. Control value for damping-off by the irrigation of sulfur solution of 1.0% and 2.0% were 75.7%, and 78.5%, respectively. Growth of leaf was inhibited by the irrigation of sulfur solution of 2.0%. Root weight per $3.3m^2$ showed the peak in sulfur solution of 1.0%, while survived-root ratio and root weight per plant were decreased in the level of 2.0%. Survived-root ratio of seedling in sulfur solution of 1.0% was distinctly increased by 4.7 times compare to the control, but control value for root rot was relatively low as 49.2%. Mycelium growth of C. destructans, F. solani, and R. solani were distinctly inhibited by the increase of sulfur's concentration in vitro culture using PDA medium.

A Study on Seedling Growth and Absorption of Inorganic Nutrients of Deutzia Crenata Seedlings Treated with Swine Manure (돈분 처리에 의한 빈도리나무 유묘생육 및 무기양분 흡수에 관한 연구)

  • Lee, Chang-Heon;Kang, Hag-Mo;Jin, Jae-Jun;Hong, Ji-Suk;Kim, Mi-Ja
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.3
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    • pp.1-12
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    • 2018
  • This study was conducted to obtain a reliable result assessing proper amount of solid swine manure for the growth of Deutzia crenata seedlings. Seedling growth dry weight, chlorophyll content inorganic nutrients and soil chemical properties were investigated. 1. When treated with solid swine manure, seed germination rate was highest on the control. However, germination rates tended to decrease when treated with swine manure at high concentrations. 2. The growth of seedlings treated with swine manure was always higher than that of in control. At the 1.0% of swine manure treatment, the growth rate of the seedlings was highest. 3. Seedling dry weight was highest at the 1.0% swine manure treatment. The amount of inorganic nutrients absorbed by the seedling was generally high with the 1.0% treatment, declined sharply with the 2.0% treatment. 4. For the planting soil of Deutzia crenata, the higher the concentration of swine manure, the lower the soil pH. However, nitrogen, available P, K, Na, Mg and Ca contents in the soil have increased with higher concentrations.

Effect of Light Transmission Ratio and Soil Moisture Content on Growth Characteristics of Seedling in Panax ginseng C. A. Meyer (투광율과 토양수분함량이 묘삼(苗蔘) 생육에 미치는 영향)

  • Lee, Sung-Woo;Kim, Chung-Guk;Hyun, Dong-Yun;Yeon, Byeong-Yeol;Lee, Kwang-Won;Cha, Seon-Woo
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.4
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    • pp.207-210
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    • 2008
  • It is very important factors to control optimal light transmission ratio and soil moisture content in order to produce good quality of ginseng seedling. To study the effect of light transmission ratio (LTR) and soil moisture content(SMC) on growth characteristics and yield of ginseng seedling, LTR was controlled by three level such as 21.2, 24.8% and 30.3%, and SMC was plotted by four level of 11.0%, 12.5%, 15.3% and 18.9% at the greenhouse. Chlorophyll content was gradually decreased in the low LTR (21.2%), while it was distinctly decreased by the decrease of SMC in excessively high LTR (30.3%). The decrease of SMC in the high LTR increased heat injury ratio distinctly, while heat injury ratio in the low LTR was only increased when SMC was very low such as 11.0%. All of fresh root weight per Kan (3.3$m^2$), root weight per plant, and the number of usable seedling were distinctly decreased by the increase of LTR and the decrease of SMC. Excessive increase of LTR in optimal SMC (18.9%) hadn't a great effect on the decrease of root weight, while root weight in low SMC was distinctly decreased by the increase of LTR. Ratio of rusty root was distinctly increased in the condition that both of LTR and SMC were high. Ratio of rusty root in the excessively high LTR was gradually fallen off by decrease of SMC, but its ratio in low SMC didn't changed distinctly by the decrease of SMC.

Nutrient Absorption and Endosperm Consumption in Rice Seedling (벼 육모일수에 따른 양분흡수와 배유양분 소모)

  • Kim, Sang Su;Choi, Min Gue;Lee, Seon Yong;Cho, Soo Yeon;Jun, Byung Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.405-410
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    • 1996
  • To elucidate the pattern of nutrient absorption and endosperm consumption as the seedling age of rice, Dongjinbyeo was raised in the seedling box with different nitrogen levels. Absorption of nitrogen and phosphorus were high in the order of artificial seed bed soil, sand with N-1g /box and sand without N from 3 and 5 days after seeding but, potassium wasn't significantly different between sand+N-1g /box and sand. Endosperm consumption rate was high in the order of artificial seed bed soil, sand + N-1g /box and sand but, endosperm dependence rate (endosperm consumption /top dry weight) was vice versa. Seedling height and dry weight were higher in the order of artificial seed bed soil, sand+N-1g /box and sand from 3 days after seeding, number of leaves were more from 5 days after seeding in same order.

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Neutralization of Rice Hull Charcoal with Nitric Acid Solution and its Neutritional Effect on Tobacco Seedling (연초용(煙草用) 상토재료(床土材料)로서의 왕겨숯(燻炭)의 질산중화효과(窒酸中和效果))

  • Lee, Y.H.;Hong, S.D.;Kim, Y.Y.;Jeong, H.C.;Kang, S.K.
    • Korean Journal of Soil Science and Fertilizer
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    • v.14 no.3
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    • pp.130-136
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    • 1981
  • Rice hull was reduced to ash by carbonization grades to illcuidate alkalinity increase and extract of inorganic nutrients in the rice hull charcoal. Alkaline reaction of water extraction was neutral at less carbonized charcoal, but much carbonized ash from 65% weight loss reached over 10 of pH value, also origin shape of rice hull was maintained until near 65% carbonized grade. Therefore, physical properties sustained good condition for seedling bed. The more charcoal carbonized to ash, the pH value and concentration of inorganic nutrient in their extracts were increased gradually. Nitric acid concentrations for neutralizing extract from charcoal were stronger in proportion to the carbonized grade but 0.1 N nitric acid solution was very reasonable to neutralize the 65% carbonized charcoal for mixing with heavy texture acidy soil(pH 5.3) of uncultivated deep horizon to transplant the tobacco seedlings. Volume ratio mixing for seedling bed is adequate at five of ash to one of acid solution. Neutralization with nitric acid solution also accelerated extraction of the inorganic nutrient in rice hull ash. Tobacco seedlings grown on bed mixed with neutralized rice hull charcoal and soil had shown better results on the agronomic measurement than alkaline ash bed, and phosphorus and cations were uptaken more amounts.

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Effects of Seeding Bed Media and Fungicide on Control of Clubroot Disease of Chinese cabbage Caused by Plasmodiophora brassicae (배추 뿌리혹병(Plasmodiophora brassicae) 방제를 위한 육묘용 상토와 농약처리 효과)

  • Hong, Soon-Sung;Kim, Jin-Young;Park, Kyeong-Yeol
    • Research in Plant Disease
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    • v.9 no.2
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    • pp.64-67
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    • 2003
  • Clubroot disease of Chinese cabbage has extremely occurred in recent years. Conventional soils such as sandy loam soil (saprolite) and clay soil (yellow soil) as bed media combined with field soil application of fungicidal chemicals were tested for the control of clubroot disease. Using sandy loam soil and clay soil as plug seedling bed media efficiently reduced clubroot disease occurrence down to 21.7% and 14.1%, respectively compared to peatmoss (75.7%) and Baroco soil (36.6%) when seedling plants were transplanted into previously-infected Yoncheon field. Application of flusulfamide and azoxystrobin to previsouly-infected soil prior to transplanting also effectively reduced disease incidence, especially when combined with growing seedlings in sandy loam or clay soil media. In conclusion, flusulfamide application prior to trnasplanting as well as utiliz-ing sandy loam and clay soil as a plug bedding media may effectively reduce the occurrence of clubroot dis-ease of Chinese cabbage.

Effect of Early Seeding on Seedling Establishment and Yield in Direct Dry Seeding Rice at Honam Plain Area of Korea

  • Kim, Sang-Su;Back, Nam-Hyun;Chot, Min-Gyu;Choi, Weon-Young;Park, Hong-Kyu;Kim, Bo-Kyeong;Shin, Hyun-Tak;Cho, Soo-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.3
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    • pp.236-242
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    • 1999
  • To examine the seedling stand and growth as affected by early seeding dates of dry direct seeded rice in the Honam plain area of Korea, Dongjinbyeo was seeded at six seeding dates from early March to late April in rice fields of silty loam soil(Jeonbuk series) at the National Honam Agricultural Experiment Station (NHAES) for two years, 1996 and 1998. Seedling stand decreased slightly. with an early seeding date, but it produced more than the optimum seedling number except for the seeding of 25 March in 1996. Days to emergence was significantly longer, as seeding date was earlier, and days to emergence by early seeding was shortened only by 8 days because the mean air temperature was lower in 1996 than average, while in 1998, the reduction effect was nine to twenty five days because the mean air temperature was higher than average. In early seeding, various weeds occurred at the emergence date of rice and dominant weeds were Alopecurus aequailis, Ludwigia prostata and Rorippa islandica. NH$_4$$^{+}$ -N content in the soil at the 5th leaf stage and maximum tillering stage were lower, as the seeding date was earlier when nitrogen was split applied as basal and top dressed in 1996, while it was not significantly different among seeding dates when nitrogen was intensively applied as a top dressing in 1998. Tiller number at the maximum tillering stage and panicle number/m$^2$ were more, as seeding date was earlier in 1996, while it was not different in 1998. Filled grain rate and 1,000 grain weight was not different among the seeding dates. Milled rice yield was significantly decreased in the seeding before the middle of March, but in the seeding after late March, it was not varied when compared with the normal seeding date in 1996, while in 1998, there was no difference among seeding dates. From the above results, in consideration of seedling stand, weed occurance, rice growth and milled rice yield, the critical optimum early seeding time in the southern plain area may lie in early April. But it was suggested that when soil moisture is proper for seeding practices, seeding amount is increased and nitrogen is applied after plumule emergence of rice, milled rice yield may not be reduced in the seeding of middle or late March, compared with the seeding in April.l.

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