The study was performed to investigate the effects of isoflavone and/or grape seed oil supplementation on serum and liver lipid profiles and bone strength in ovariectomized female rats. Female Spraque-Dawley rats were assigned into 4 groups of ovariectomized (O), isoflavone (0.085 mg/100 g b.w/day) in ovariectomized rats, grape seed oil in ovariectomized rats and isoflavone and/or grape seed oil in ovariectomized rats. After 8 weeks, biochemical profiles of serum, liver and bone were analyzed. Total food intakes, body weight gains and FER (food efficiency ratio) were not statistically significantly different among groups. Total cholesterol, triglyceride and LDL-cholesterol levels in serum were decreased by fed of isoflavone and/or grape seed oils. However, crude lipid and total cholesterol contents in liver were not affect of isoflavone and/or grape seed oil. The hepatic glutathione contents were increased by isoflavone and/or grape seed oil fed. The hepatic glutathione-S-transferase activity in isoflavone and/or grape seed oil supplemented groups were higher than that O group. Bone (scapular and femur bone) dry weight, femur of max weight and bending strength were no significant difference among groups. Our finding suggest that isoflavone/grape seed oils might have potential role for serum lipid profiles improvement and bone strength in vivo.
In order to obtain the genetic information on the quantitative characters of black seeded soybeans, which would be needed to improve selection efficiency for breeding high yielding genotype, 45 varieties of black seeded soybeans collected in Chonnam, Korea were grown and variations of several important characters were observed. Heritability of each observed character, phenotypic and genotypic correlations among the characters and contribution of each yield component on grain yield through path coefficient analysis were estimated. Both number of pods per plant and 100-seed weight showed not only high heritability but also highly significant phenotypic and genotypic correlation with seed yield, and hence it was desirable to select plants with more number of pods per plant and higher 100-seed weight than raise seed yield of black seeded soybeans collected in Chonnam. In addition, number of pods per and 100-seed weight were proved to be the most influential variables on the viability of seed yield by path coefficient analysis. Since these showed the high heritability of number of pods per plant, selection of plants with higher 100-seed weight would be more efficient for breeding high yielding genotype.
These experiments were conducted to investigate the effect of day length on some chracteristics and seed weight in the soybean cultivars. Those were classified into three type's of seed weight with delaying seeding date in the previous paper. Flowering was delayed as the longer day length was employed. When the length became longer. the number of leaves at flowering greatly length of main stem and the number of nodes at maturity greatly increased. The seed weight decreased when the day length was longer than 14 hours. Negative correlation was obtained between seed weight and day length. There was negative correlation between seed weight and the number of days required to flowering, and length of main stem and the number of nodes at maturity. Type I cultivars decreased in seed weight when the day length was longer than 16 hours, but this trend was found to be clear with type II cultivars. Type III cultivars decreased in seed weight with increasing the day length.
The effect of water deficits on soybean [Glycine max (L.) Merr.] could appear on seed quality through changes of morphological plant characteristics. Two Korean genotypes, Hwangkeum (determinate growth habit) and Muhan (indeterminate growth habit), were used to examine the influences of treatment stage and method of water deficit during reproductive growth period on yield and seed quality of soybean. Water deficit at R5 or R6 stages was as damaging to seed quality as double water-deficit treatments at R2+R5 or R2+R6. However, seed from double water-deficit treatment tended to have lower oxidation-reduction potential compare to the corresponding single water-deficit treatment. In comparison with Muhan, Hwangkeum had significantly greater oxidation-reduction potential value. Seed yield per plant in both genotypes depended greatly on seed yield of branches. However, the proportion of number of branch seed to total seed umber in Hwangkeum was increased as the water deficit was applied during later reproductive stage, whereas, in Muhan the proportion was lower. Water-deficit treatments including the single and double water-deficit treatments and non-stressed treatment were able to be classified into five groups for Hwangkeum and four groups for Muhan based on the influences on yield components, number of pod, number of seed, and single seed weight, using principal component analysis. In both genotypes, R2+R5 water-deficit treatment decreased number of pod and seed, but increased single seed weight. On the contrary, R6 or R2+R6 stress increased the pod and seed number, but decreased single seed weight.
The present experiment was conducted to investigate the effects of seeding date on agronomic characters including seed weight of soybean (Glycine max (L.) Merr.) at Sunchon, the southern coastal area of Korea. One hundred eighteen native and improved varieties were used in this study. As the seeding date was delayed, the number of days to flowering for the cultivars was reduced. This trend was more obvious in late maturing cultivars(LMC) than in early and medium maturing cultivars (EMC and MMC). Late seeding also resulted in decrease in the number of leaves, stem length, and number of nodes. The heaviest seed weight was obtained with EMC and MMC planted on May, and seed weight decreased with delayed seeding date. Seed weight was positively correlated with number of days to flowering, number of total leaves at flowering, stem length and number of nodes on main stem at maturity. Based on seed weight the cultivars was classified into five types: Type I; Seed weight of the cultivars decreases with delayed seeding date. Type II; Seed weight of the cultivars does not vary with seeding date. Tyep III; Seed weight of the cultivars increases with delayed seeding date. Type IV; Seed weight of the cultivars increases when the seeding date approached the appropriate seeding date, but decreases thereafter. Type V; The reversed type IV. Type I, II, III, IV and V occupied 37, 16, 17, 10 and 20% of the tested cultivars, respectively.
This study is to find out the antioxidative effect and serum lipid composition of wild grape seed powder in vivo. 20 white Sprague Dawley rats of six weeks old were divided into 2 groups and AIN-93 basic diet, high fat and cholesterol were provided. And they were examined to know how wild grape seed powder worked for antioxidative effect and serum lipid composition. For the comparing group, wild grape seed powder consisting 5% of the diet weight was provided and the quantity of protein, fat, carbohydrate, and cellulose was controlled following the analysis of the ingredients. The rats were fed for four weeks with experimental diet. Serum lipid and the antioxidant enzyme activity in blood and liver microsome were measured after 4 weeks of experiment. The results are as follows; There was no difference between the experimental groups in the initial body weight, final body weight, weight gain and FER. Food intake was higher in the group wild grape seed powder was provided than in the control group(p<0.05). Serum total cholesterol in the control group was significantly higher than that in the group wild grape seed powder was provided.(p<0.05). There was no difference serum HDL cholesterol and LDL cholesterol between the groups. Serum triglyceride showed no significant difference between the groups. In blood, glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group. The glutathione reductase activity of blood showed no difference between the groups. In liver, the glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group(p<0.05). Glutathione reductase activity in liver showed no difference in accordance with the supplementation of wild grape seed powder.
Yu-Mi Choi;Hyemyeong Yoon;Myoung-Jae Shin;Yoonjung Lee;On Sook Hur;XiaoHan Wang;Kebede Taye Desta
Proceedings of the Plant Resources Society of Korea Conference
/
2021.04a
/
pp.15-15
/
2021
Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.
This study was carried out to provide basic information for mass breeding and cultivating the saplings of Camellia japonica. The germination rates did not show definite tendency according to the changes of seed weight at room temperature and 4℃ dry storage conditions. The germination rate of 4℃ wet sand storage was increased with seed weight, but showed very low germination rate for 120 days of storage. The germination rate was above 80% in 60 days, 90 days and 120 days, respectively, at 4℃ wet filter paper storage conditions. The average days taken for those germinations were about 30 days at room temperature and 4℃ dry storage conditions, but wet storage (sand, filter paper) condition took the shortly nearly 13 days for those germinations. Leaf number, leaf dry weight, stem diameter and root dry weight were higher than total average by more than 1.21 g for seed weight. Stem length was higher than the average by more than 1.01 g, plant dry weight was higher than the average by more than 0.81 g, and stem dry weight was higher than the average by more than 0.61 g. In the case of seed weight becoming heavier, seedling growth was good but T/R ratio tended to decrease. In order to increase the mass production of seedlings using Camellia japonica seeds, we need to specify the weight and size of those seeds. The 4℃ wet filter paper storage condition was evaluated as the most efficient method for the seed storage used for seeding.
The experiment was conducted to get basic information for cotton breeding program through four-variety diallel cross population. Additive, dominant, maternal, and reciprocal effect were observed significantly for days to maturity. Based on the Vr-Wr graphical analysis, the characters, number of bolls per plant, boll weight, seed and lint weight per boll, lint weight per boll, width of seed, 100-seed weight, days to flowering, and days to maturity were found to inherit incomplete dominance, and the characters of number of bolls per plant, width of seed, number of seeds per boll, and length of seed were assumed to inherit over dominance. The component of genetic variance analysis for boll weight, seed and lint weight per boll, lint weight per boll, number of seeds per boll, length of seed, and days to flowering showed that additive effects were higher than dominant effects. The narrow-sense heritability(h$_2$N) for boll weight, seed and lint weight per boll, lint weight per boll, days to flowering, and days to maturity showed high values as more than 43.19%. The estimate of broad-sense heritability(h$_2$B) value was higher than that of h$_2$N because of the low importance of dominance effects.
Park, Moon-Woong;Chae, Je-Cheon;Kim, Jung-Gon;Lee, Choon-Ki
KOREAN JOURNAL OF CROP SCIENCE
/
v.39
no.1
/
pp.55-62
/
1994
The germplasms of naked barley evaluated for starch content were 137 crossing blacks and progeny lines obtained from the breeding program of the Wheat and Barley Research Institute, 540 Tabdong Kwonsungu Suwon, in the 1988/1989 season. Starch content was distributed between 49.15 and 62.50% with a mean of 57.20%. It was high in the bred lines and the Uz type lines than the imported, uz type or lax type ones. The range observed for seed coat weight was 1.12-3.02mg and the mean seed coat weight was 1.88mg. The seed coat weight ratio to kernel weight was measured between 4.0 and 8.7% with a mean of 6.5%. The varieties differ greatly in the characteristics depending on the plant type and origin of germplasms. The variation observed with protein content ranged from 9.60 to. 15.90% while the average was 12.86%. Starch content was negative relationships with kernel weight, seed coat weight, seed coat weight ratio., kernel width, kernel thickness and protein content, and starch content was higher in smaller kernels within a variety. The weight and weight ratio. of seed coat were positively correlated with the width, thick-ness of kernels, whilst they were negatively correlated with starch content. The heritability of starch content was 30.0%.
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