• 한국작물학회지
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• 제48권5호
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• pp.372-375
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• 2003

In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition and their nutritional value were observed. Generally, rape was considered as a useful forage fodder crop with high content of crude protein and low contents of NDF, ADF, hemicellulose, cellulose and lignin. Differences in mean values of the above characters between two groups of rape were not statistically significant. Velox showed significantly higher content of crude protein and significantly lower contents of NDF, ADF, hemicellulose, cellulose and lignin compared with other varieties of forage rape. Rape was relatively high in IVDMD compared with other forage fodder crops, and forage rape was more or less higher in IVDMD and DDMM than oil seed rape. Velox was the highest in IVDMD and DDMM among the varieties of forage rape in this experiment.

• 한국생활과학회지
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• 제21권2호
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• pp.377-388
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• 2012

The purpose of this study was to determine the content attributes and sensory characteristics of ice cream flavored with lotus leaf and seeds (Nelumbo nucifera Gaertner). Analysis of freeze dried lotus leaf powder produced the following technical results: Freeze dried lotus leaf powder contained moisture 12.2, crude protein 5.9, crude fat 1.2, crude ash 7.0, crude fiber 13.4g/100mg; raw lotus seed powder contained moisture 65.0, crude protein 8.2, crude fat 0.3, crude ash 1.0, crude fiber 10.8g/100mg; freeze dried lotus seed powder contained moisture 14.0, crude protein 17.1, crude fat 1.9, crude ash 4.0, crude fiber 2.8g/100mg. Lotus seed was processed by 4 methods: freeze dried, roasted, freeze dried peeled inner layer, cooked cut peeled inner layer. Analysis of the color value produced the following technical results: freeze dried lotus leaf powder were L(lightness) 14.5, a(redness) 4.4, b(yellowness) 24.0; freeze dried lotus seed powder L 57.3, a 14.4, b 12.0; roasted lotus seed powder L 52.7, a 22.5, b 11.9; freeze dried lotus seed peeled inner layer L 60.0, a 1.4, b 12.3. Proportional lotus ingredients used for ice cream were 1%, 3%, 5%, 7%, 10% for lotus leaves and 1%, 3%, 5% for lotus seeds. The maximum over run ratio for lotus leaf ice cream was 45.3~56.9% at 20~25 minutes operating time. Over run was significantly decreased by increasing the contents of lotus leaf powder. A maximum over run ratio for lotus seed ice cream was 46.1~54.3% at 20~25 operating time. Over run was not significantly different based on content of lotus leaf versus lotus seed. Sensory evaluation of lotus leaf ice cream produced the following results: the highest score was for color of 4.42 and the lowest score was for sweetness of 3.30. The total mean score significantly decreased by increasing the content of lotus leaf powder. Sensory evaluation of lotus seed ice cream produced the following results: the highest score was for cooked cut peeled inner layer type and the lowest was for freeze dried type 3.86. The sensory scores were not significantly different based on the content of lotus seed. Texture was evaluated highest with a rating of 4.21 and the taste was evaluated lowest with a rating of 3.68 For whole evaluation. This study concluded that lotus leaf ice cream demonstrates relative strength in terms of color and taste, and a relative weakness in terms of over run and sweetness. Suitable content of lotus leaf ranged from 1~5%. The lotus seed processed by the cooked cut peeled inner layer method received a higher rating than dried powder for lotus seed ice cream.

• 한국식품과학회지
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• 제10권1호
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• pp.83-87
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• 1978

한국산 호박씨중에서 지방(脂肪)을 추출하여 지방산(脂肪酸)의 종류를 조사하였고 또한 물에 가용(可溶)인 단백질(蛋白質) 및 불용성(不溶性) 단백질(蛋白質)을 분리(分離)하여 Disc 전기영동으로 단백질의 Pattern을 확인하고 아미노산 조성(組成)을 관찰하였다. 1. 호박씨의 일반성분중 지방은 46.5% 단백질 25.5%를 함유하고 있다. 2. 호박씨기름의 지방산(脂肪酸) 조성(組成)은 linoleic acid 51.74%, oleic acid 22.2%, palmitic acid 18.1%, stearic acid 7.84%로 이들 4가지를 주 지방산으로 하고 있다. 3. 호박씨 단백질(蛋白質)의 아미노산(酸) 조성(組成)은 tyrosine, glutamic acid, aspartic acid, leucine, glycine등을 다량(多量) 함유하고 있으나 함황(含黃) 아미노산(酸)은 함유(含有)하고 있지 않다. 4. 단백질의 Disc전기영동 결과 가용성(可溶性) 단백질(蛋白質)에서 13개의 band와 불용성(不溶性) 단백질에서 4개의 band가 분리 되었다.

• 한국식품영양과학회지
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• 제23권4호
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• pp.647-653
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• 1994

This study was conducted to investigate the contents of dietary fiber (DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food source such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F) , acid detergent fiber( A.D.F) . kignin, hemicellulose an dcellulose in evening primorse seed were higher than those of other samples, except the content of cellulose .The ration of polyunsaturated /saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88% , which was higher than those of other samples. The fatty acid composition in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the sameas those of glycolipids. The contents of PUFA in Platycodi radix were 62.96% . The essential amino acid contents of salt-soluble protein were 47 mole % in Platycodi radix , which was slightly higher than those other samples. The ration of essential amino acid /nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.

• 한국작물학회지
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• 제59권3호
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• pp.245-251
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• 2014

우리나라 수수 유전자원 20품종에 대한 종자 단백질의 정량적 및 정성적 특성을 분석하였다. 수수 종자의 수용성 단백질의 분획은 'Osborne 방법'의 변형된 방법으로서 알부민, 글로불린, 프롤라민 및 글루텔린을 순차적으로 분리하여 정량분석을 실시하였다. 수수 종자의 저장단백질인 프롤라민 단백질의 조성을 1차 전기영동으로 비교 분석하였다. 1. 총 단백질 함량 대비 분획단백질의 함량은 알부민 단백질은 6.2%, 글로불린 단백질은 0.9%, 프롤라민 단백질은 57.9% 및 글루텔린 단백질은 35.1%의 함량을 나타내었다. 2. 품종별 비교에서 프롤라민 단백질의 함량이 높은 품종은 흰수수, 흰찰수수, 황금찰수수 및 대풍수수로 결정되었다. 3. ${\alpha}$-kafirin의 함량이 높은 품종은 흰수수, 금산찰수수, 흰찰수수 및 장목수수로 나타났다. 4. 흰수수와 흰찰수수가 고 프롤라민 및 고 ${\alpha}$-kafirin 품종임을 제시하였다.

• 한국식품과학회지
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• 제36권6호
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• pp.1026-1031
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• 2004

들깨 메탄올 추출물이 acetylcholinesterase(AChE)의 활성 및 PC 12 cell 세포사멸에 미치는 영향을 검토하였다. AChE에 대한 억제환성은 들깨 메탄을 추출물의 농도가 높을수록 유의적으로 높았으며 추출물의 분획물 중에서는 n-butanol층이 가장 높은 억제율을 나타내었다. L-glutamate 또는 ${\beta}-amyloid$ protein $(A{\beta})$으로 유도된 PC 12 cell에 대한 세포사멸 억제효과도 추출물의 농도가 높을수록 유의적으로 증가하였다. 또한 rat brain에 $FeSO_{4^-}H_2O_2$로 산화적 스트레스를 유발시켜 추출물의 TBARS 생성 억제활성을 조사한 결과 들깨 메탄을 추출물은 농도가 높아질수록 산화적 스트레스에 대한 억제활성이 증가하였으며 분획물 중에서는 n-butanol층에서 가장 높은 억제활성을 나타내었다. 이상의 결과들로 미루어 볼 때 들깨 메탄을 추출물은 AChE 활성을 억제하고 glutamate 또는 $A{\beta}$에 의하여 유도된 PC12 cell의 세포사멸을 억제하며 이러한 효과는 들깨 메탄을 추출물의 항산화력에 기인할 수도 있을 것으로 사료되었다.

• 한국작물학회지
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• 제38권1호
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• pp.15-22
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• 1993

들깨 국내 수집 유전자원 80종에 대한 종실의 단백질 함량과 아미노산 조성을 분석 조사하여 고단백 양질 아미노산 품종육성에 오필요한 기초자료를 얻고자 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 들깨 종실의 단백질 함량은 최고 28.5%, 최저 17,9%, 평균 24.6%였다. 2. 단백질 함량은 숙기와 천입중에 따라서는 큰 차이가 없었으나 종피색에서는 암갈색에서 백색에 가까울수록 높았는데 암갈색에 비해 은회색은 1.4% 더 높았다. 3. 들깨 수집 유전자원 80종의 총 필수아미노산은 품종에 따라 최고 44.85%, 최저 36.41%, 평균 40.55% 였으며 일반 아미노산 함량은 최고 60.01%, 최저 47.23%, 평균 55.29%였다. 4. 필수 아미노산 함량과 종피색간에는 고도의 정상관(r=0.3389＊＊) 관계로서 종피색이 암갈색에서 백색에 가까울수록 높은 경향이었다. 5. 들깨에서 필수 아미노산중 함량이 많은 것은 arginine과 leucine, 가장 적었던 것은 methionine 이었고, 일반 아미노산중 함량이 많은 것은 glutamic acide와 asparagine이었다. 6. 필수아미노산 중에서 lysine, methionine, valine 등의 평균함량은 FAO 권장량 보다 높았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.22-22
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• 2019

Green stem disorder (GSD) of soybean (Glycine max (L.) Merr.) is characterized by delayed senescence of stems with normal pod ripening and seed maturation (Hobbs, 2006). GSD complicates harvesting of soybeans by significantly increasing the difficulty in cutting the affected plants. There is also the potential for moisture in the stems to be scattered on the seed, reducing the grade and storability of the seed. Not only the cause of GSD is yet unknown, but also GSD cannot be evaluated until maturity, therefore the method to evaluate GSD in early growth stage with high sensitivity is necessary. In previous studies, it has been reported that vegetative storage protein (VSP) accumulates and the syndrome of GSD appears in soybean after depod treatment (Fischer, 1999). Soybean VSP is a storage protein which is abundant in young sink leaves and degraded during seed fill (Wittenbach, 1982). Hence, we have established a system to quantify VSP of high sensitivity by using standard protein made by genetically transformed E. coli and specific antibody against VSP, and studied the relationship between VSP and GSD, by depod experiment and drought/excess wet experiments. The result of depod experiment with the cultivar 'Yukihomare' was the same with the previous studies, VSP accumulated much more than control and the syndrome of GSD appeared in soybean in depod treatment. Drought and excess wet had different impact on GSD. Excess wet caused GSD of the cultivar 'Tachinagaha (GSD susceptible)', while drought caused a little syndrome of GSD in the cultivar 'Touhoku 129 (GSD resistant)'. The accumulation of VSP differed between the two cultivars over time. In conclusion, the accumulation of VSP came along with the emergence of GSD. Different cultivars showed different response to drought and excess wet. In the future, it is expected that the dynamics of VSP will be elucidated in detail, leading to the development of early diagnosis technology for green stem disorder and the elucidation of mechanism of soybean GSD.

• 한국작물학회지
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• 제59권3호
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• pp.359-363
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• 2014

Soybean seed is a good source of plant protein in human consumables such as baby formula and protein concentrate. The seeds contain an abundance of storage proteins, namely ${\beta}$-conglycin and glycinin that account for ~ 70-80% of the total seed protein content. Proteome profiling has been proved to be an efficient way that can help us to investigate the seed storage proteins. In the present study, the seeds were removed from the pods and the cotylendonary tissues were separated from the testa for proteome analysis in order to investigate the seed storage proteins. A systematic proteome profiling was conducted through one-dimensional gel electrophoresis followed by MALDI-TOF-TOF mass spectrometry in the seeds (cotyledonary tissue) of soybean genotypes. Two dimensional gels stained with CBB, a total of 10 proteins were identified and analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. A total of ten proteins such as glycinin Gy4 precursor, glycinin G3 precursor, glycinin G1 precursor, glycinin chain A2B1a precursor, glycinin chain A2B1a precursor were identified in our investigation. However, the glycinin subunit may be considered to play important roles in soybean breeding and biochemical characterization. In addition, the improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

• 한국작물학회지
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• 제45권5호
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• pp.339-342
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• 2000

Near-infrared reflectance spectroscopy (NIRS) was used to estimate the lipid and protein contents in ground seed samples of perilla (Perilla frutescens Brit.) and peanut (Arachis hypogaea L.). A total of 46 perilla and 80 peanut calibration samples and 23 perilla and 46 pea. nut NIRS validation samples were used for NIRS equation development and validation, respectively. Validation of these NIRS equations showed a range of very low bias (-0.05 to 0.13 %) and standard error of prediction corrected for bias (0.224 to 0.803%) and very high coefficient of determination ($R^2$) (0.962 to 0.985). It was concluded that NIRS could be adapted as a mass screening method for lipid and protein contents in perilla and peanut seed.