• Title/Summary/Keyword: seed germination test

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Evaluating germination of lettuce and soluble organic carbon leachability in upland sandy loam soil applied with rice husk and food waste biochar (왕겨 바이오차 및 음식물쓰레기 바이오차가 밭 사양토에서 상추발아 및 수용성 유기탄소 용출에 미치는 영향 평가)

  • Han, Kyung-Hwa;Zhang, Yong-Seon;Jung, Kang-Ho;Cho, Hee-Rae;Sonn, Yeon-Kyu
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.369-377
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    • 2014
  • This study was carried out to evaluate the effect of rice husk (RHB) and food waste biochar (FWB) on upland soil with sandy loam texture, in terms of physico-chemical analysis, lettuce seed germination test, and orgainc carbon leaching experiment. RHB and FWB had different physico-chemical properties each other. Carbon to nitrogen ratio (C/N ratio) of RHB was 32, showing two times higher than that of FWB. FWB had high salt and heavy metal content, compared to RHB. This is probably due to different ingredients and production processing between two biochars each other. Results of germination test with Lettuce showed lower germination rate when FWB was applied because of higher salt concentration compared to control and RHB. Organic carbon leaching test using saturated soil column (${\Phi}75{\times}h75mm$) with $10MT\;ha^{-1}$ biochar application rate, showed higher saturated hydraulic conductivity in rice husk biochar treatment column, compared to control and food waste biochar treatment. The highest total organic carbon concentration in column effluent was lower than those in both of rice husk biochar and food waste biochar, whereas the differences was negligible after 9 pore volumes of effluent. Consequently, biochars from byproducts such as rice husk and food waste in sandy loam textured upland soil could enhance a buffer function such as reduction of leaching from soil, but the harmful ingredient to crops such as high salt and heavy metals could limit the agricultural use of biochars.

Effective Screening Condition for Low-Temperature Germinability of Rice (벼 저온발아성의 효율적 검정 조건)

  • Kang, Jong-Re;Lim, Sang-Jong;Kim, Soon-Chul;Ko, Mi-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.711-715
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    • 1995
  • This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

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Effect of Iron-coated Seed on a Healthy Rice Seedling Raising Nursery in Machine Transplanting (Growth Chamber에서 철분코팅 벼 종자의 발아 및 유묘생육 특성)

  • Park, Kwang Ho;Kim, Yang Sik;Chang, Jin Tack
    • Journal of Practical Agriculture & Fisheries Research
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    • v.16 no.1
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    • pp.17-24
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    • 2014
  • The experiment was conducted at the growth chamber to determine germination, seedling growth and fresh weight between the untreated control and iron-coated seeds in germination test of the rice seeds. There was of 95% germination in rice seeds regardless of treatments and days for seedling emergence was relatively 13-14days. Seedling height was 4.9cm of the iron-coated seeds but 0.4cm of the untreated control and there was 6.S roots of seedling in iron-coated seeds and 5.5 roots of seedling in the untreated control, respectively. The fresh weight of seedling was 107mg of the iron-coated seeds and 66mg of the untreated control at 35days after sowing.

Effect of Chemical Treatments on Seed Germination of Saururus chinensis (Lour.) Baill., an Endangered Species in Korea (멸종위기 야생식물 삼백초의 종자발아에 미치는 화학적 처리의 영향)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.29 no.4
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    • pp.385-392
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    • 2016
  • This research was performed to develop seminal propagation method of Saururus chinensis (Lour.) Baill. by conducting a rigorous germination study. Well-selected seeds were dry-stored at 4 ± 1.0℃ during the experiment. To study dormancy type, non-stored seeds were analyzed by embryo observation, germination test and detecting for any difficulties in seed coat to absorb moisture. Then to improve germination, seeds were submerged for 24 hours in a solution of varying concentrations containing one of plant growth regulators and minerals. According to research, fleshly matured seeds had an undifferentiated embryo and had a low germination rate below 5%. In addition, water submersion led to moisture absorption, embryo in the seeds grew and germinated so it was deemed morphophysiological dormant seeds. Percent germination (PG) and germination energy (GE) was greatly improved by soaking in plant growth regulators and minerals for 24 hours. Especially, 500 ㎎/L GA3 treatment resulted in the highest GE as 46.1%. KNO3 meaningfully improved PG (54.3∼57.7%) at 10∼20 mM but effect of minerals on germination acceleration as GE were negatively impacted in all concentrations.

Viability Determination of Pinus rigida Seeds Using Artificially Accelerated Aging (노화처리를 이용한 리기다소나무 종자의 활력 평가)

  • Han, Sim-Hee;Koo, Yeong-Bon;Kim, Chan-Soo;Oh, Chang-Young;Song, Jeong-Ho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.1
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    • pp.10-14
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    • 2006
  • We tested the seed viability of Pinus rigida using accelerated aging to discover optimum times and temperatures far artificially accelerated aging. Seeds were artificially aged at different temperatures and during different tines. The seed viability was affected by the accelerated aging and by temperature with a decline in germination and seed vigor. The aging index of P. rigida seed was 0.31 at $35^{\circ}C$ and seed viability was nearly lost after aging treatment at $40^{\circ}C$ for 15 days. The optimum temperature of P. rigida far the aging test was decided to be approximately $37^{\circ}C$ on the basis of the aging index. Inorganic materials and conductivity of leaching solution from aging seeds increased with the increase of aging period. The accelerated aging test was considered to be a suitable method to evaluate the seed viability of tree species. Because seed characters are much different among tree species, however, more studies need to be done to discover the optimum conditions for aging by tree species.

Effect of Water Uptake Rate on Germination Characteristics of Waxy Rice Seeds and Guaiacol Peroxidase Activity during Early Imbibition (종자의 수분흡수속도가 찰벼 품종별 발아특성과 침윤초기 Guaiacol Peroxidase활성에 미치는 영향)

  • Shon, Jiyoung;Kim, Junhwan;Lee, Chung-Kuen;Yang, Woonho;Yoon, Young-Hwan;Chung, Nam-Jin;Kim, Chung-Kon;Kim, Bo-Kyung;Choi, Kyung-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.4
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    • pp.416-423
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    • 2013
  • Germination is controlled by internal factors of seeds and external factors such as water, temperature and light. We investigated the relationship between germination characteristics of four waxy rice cultivars and patterns of water uptake, antioxidant enzymes and free soluble sugars during early imbibition. Seed viabilities by tetrazolium test of four different rice cultivars were higher than 95% and germination rates of the hulled rice seeds were on 95% average. However, germination rate of intact rice among four cultivars showed a big difference depending on temperature. Water uptake of hulled and intact rice seeds during imbibition reached a stationary phase at around 30% moisture content. Although rates of water uptake were faster in hulled rice and high temperature than intact rice and low temperature condition, difference of those among cultivars was greater under low temperature than high temperature. The time required for rice seeds to uptake 30% water was negatively correlated with percentage of germination, germination energy, germination speed and mean germination time. Guaiacol peroxidase activity at 24h of imbibition was correlated with germination energy and germination speed but not percentage of germination. Catalase activity, soluble protein and maltose concentration at 24h of imbibition were not correlated with characteristics of germination. These results suggest that a time required for rice seeds to uptake 30% of water significantly correlated with germination and guaiacol peroxidase activity during early imbibition plays an important role in initiation of germination.

Germination of Gourd Seed Affected by Drying and Light Quality Treatments during its Period (건조방법과 건조중 광질처리가 박 종자의 발아에 미치는 영향)

  • 강진호;강신윤;이상우;윤수영
    • Journal of Life Science
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    • v.13 no.1
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    • pp.15-20
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    • 2003
  • Seeds treated before sowing should be dried for their quality control on their sale or storage. The experiments were done to clarify the effect of drying, temperature and light quality treated during drying and germination on germination of gourd (Lagenaria siceraria Standl.) seeds. Drying of treated seeds was done at $35^{\circ}C$. After treated seeds were dried. their germination test was done at 10, 20 and $30^{\circ}C$ on the condition of darkness, but with blue, red, far-red light and darkness as light quality treatments. FR-yongjadaemok and FR-kunghap were used as test cultivars and their daily germination rates were measured at treatment levels. Its presowing hydrated seeds were dried 10 hours at $35^{\circ}C$ until reached below their original moisture content, and germination rate between the above dried seeds and the conventional dry-heated ones was not significantly different. At $30^{\circ}C$ germination temperature, the treated seeds showed higher initial germination rate when dried under red light illumination than under darkness although their germination rates were nearly same at each temperature of 10 and $20^{\circ}C$ being relatively low. Effect of the red light treatment during their drying continued to more than 3 months. Germination rate of the red light treated seeds during drying was reduced by far-red light treated during their germination. The initial rate to the second day after sowing were higher under red light and darkness treated during germination than under the two other lights.

Native Plants Combination for Ecological Environmental Restoration of the Dissected Sloping Area (절개지 사면의 생태환경 복원을 위한 자생식물 조합)

  • Lee, Chang Shook;Suh, Hyoung Min;Kim, Dong Geun;Eum, Sang Mi;Choi, Sun A;Lee, Nam Sook
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.6
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    • pp.36-50
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    • 2009
  • To suggest the native plants combination for restoration of the dissected sloping area, the plant communities of thirty seven quadrats from five localities of mid-southern part of Korean peninsula were surveyed from 2007 to 2008. Their flora, vegetation structure, and dominant species based on the importance value were investigated. And the soil characters (pH, moisture content, water holding capacity, and organic matter) of each dominant species were analyzed. Also, germination tests were performed to check the stability of restored native plants using seed chips. As a result, 79 native plants were suggested for the dissected sloping area : 20 trees and subtrees for the upper layer, 18 shrubs and vines for the middle layer, and 41 herbs for the lower layer, taken together their flora, dominant species, vegetation structure, soil condition, germination test, flowering period.

Effect of Herbicide Quinclorac on Ginseng Growth (제초제 Quinclorac이 인삼생육에 미치는 영향)

  • 이일호;김명수
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.96-100
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    • 1996
  • In this experiment, germination test of ginseng seed and growth test of two year old ginseng in soil incorporated with quinclorac (3,7-Dichloro-8-quinoline carboxylic acid) were conducted to evaluate phytotoxicity caused by quinclorac residue in soil. Ginseng seed germiilation was hardly affected, but growth of ginseng seedling was retarded by quinclorac. The phytotoxicity of quinclorac on ginseng seedling was expressed at the lowest level tested, 3.75 g a.i./10a (118 of recommended rate for rice cultivation). The representative phytotoxicity in ginseng were decreased root weight, shortened leaf width and leaf length, and weak lateral root Quinclorac residue decreased by 1/4 in pot soil after 2 months and below 2 ppm in the paddy soil applied at usual rate (30 g a.i./10a) after 1∼ 2 years. Below 2 ppd ginseng did not show any phytotoxicity symptom.

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Allelopathic and Autotoxic Effects of Alfalfa Plant and Soil Extracts

  • Chon, Sang-Uk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.7-11
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    • 2004
  • Alfalfa (Medicago sativa L.) plants have been reported to be autotoxic as well as allelopathic. Laboratory and greenhouse experiments through petri-dish and pot test were conducted to determine autotoxic effects of alfalfa leaf and soil extracts on the germination or early seedling growth of alfalfa, and to evaluate allelopathic effects of alfalfa leaf residues on alfalfa, barnyard grass, com, eclipta and soybean. Alfalfa seed germination was delayed depending on aqueous extract concentration, with no difference in final germination after 48 hours. Alfalfa root length was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Root growth of alfalfa was significantly inhibited at extract concentration of more than 1 g dry tissue/L (g $\textrm{L}^{-1}$). Hypocotyl growth, however, was not affected by all the concentrations of leaf extracts. Soil extracts from 4-yr-old alfalfa stand significantly reduced alfalfa root length by 66%, while soil extracts from 0,1, and 3yr-old stand stimulated root length up to 14-32% over the control. Residue incorporation with dry matters of alfalfa leaf at 100 g $\textrm{kg}^{-1}$ reduced seedling length of several crop and weed species, ranging from 53 to 87% inhibition. Addition of nutrient solution into alfalfa leaf extracts alleviated alfalfa autotoxic effect. This result indicates alfalfa leaf and soil extracts or residues could exert autotoxic as well as allelopathic substances into soil environments during and after establishment.