KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Allelopathic and Autotoxic Effects of Alfalfa Plant and Soil Extracts

  • Chon, Sang-Uk (Biotechnology Industrialization Center, Dongshin University)
  • Published : 2004.03.01
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Abstract

Alfalfa (Medicago sativa L.) plants have been reported to be autotoxic as well as allelopathic. Laboratory and greenhouse experiments through petri-dish and pot test were conducted to determine autotoxic effects of alfalfa leaf and soil extracts on the germination or early seedling growth of alfalfa, and to evaluate allelopathic effects of alfalfa leaf residues on alfalfa, barnyard grass, com, eclipta and soybean. Alfalfa seed germination was delayed depending on aqueous extract concentration, with no difference in final germination after 48 hours. Alfalfa root length was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Root growth of alfalfa was significantly inhibited at extract concentration of more than 1 g dry tissue/L (g $\textrm{L}^{-1}$). Hypocotyl growth, however, was not affected by all the concentrations of leaf extracts. Soil extracts from 4-yr-old alfalfa stand significantly reduced alfalfa root length by 66%, while soil extracts from 0,1, and 3yr-old stand stimulated root length up to 14-32% over the control. Residue incorporation with dry matters of alfalfa leaf at 100 g $\textrm{kg}^{-1}$ reduced seedling length of several crop and weed species, ranging from 53 to 87% inhibition. Addition of nutrient solution into alfalfa leaf extracts alleviated alfalfa autotoxic effect. This result indicates alfalfa leaf and soil extracts or residues could exert autotoxic as well as allelopathic substances into soil environments during and after establishment.

Keywords

References

  1. Abdul-Rahman, A. A. and S. A. Habib. 1989. Allelopathic effect of alfalfa (Medicago sativa) on bladygrass (lmperata cylindrica). J. Chem. Ecol. 15:2289-2300 https://doi.org/10.1007/BF01012082
  2. Bhowmik, P. C. and J. D. Doll. 1984. Allelopathic effects of annual weed residues on growth and nutrient uptake of corn and soybean. Agron. J. 76 : 383-388 https://doi.org/10.2134/agronj1984.00021962007600030008x
  3. Chon, S. U. and S. K. Choi. 2000. Evaluation of alfalfa autotoxicity on germination and early seedling growth of 3 cultivars. Plant Res. 3(1) : 66-70
  4. Chon, S. U., J. H. Coutts, and C. J. Nelson. 2000. Effects of light and growth media on a seedling assay of alfalfa autotoxicity.Agron. J. 92 : 715-720 https://doi.org/10.2134/agronj2000.924715x
  5. Chung, I. M. and D. A. Miller. 1995a. Effect of alfalfa plant and soil extracts on germination and seedling growth. Agron. J. 87: 762-767 https://doi.org/10.2134/agronj1995.00021962008700040025x
  6. Chung, I. M. and D. A. Miller. 1995b. Difference in autotoxicity among seven alfalfa cultivars. Agron. J. 87 : 596-600 https://doi.org/10.2134/agronj1995.00021962008700030034x
  7. Dornbos, D. L., Jr., and G. F. Spencer. 1990. Natural products phytotoxicity. A bioassay suitable for small quantities of slightly water-soluble compounds. J. Chern. Ecol. 16 : 339-351 https://doi.org/10.1007/BF01021769
  8. Ells, J. E. and A. E. McSay. 1991. Allelopathic effects of alfalfa plant residues on emergence and growth of cucumber seedlings. HortScience 26 : 368-370
  9. Hedge, R. S. and D. A. Miller. 1990. Allelopathy and autotoxicity in alfalfa: characterization and effects of preceding crops and residue incorporation. Crop Sci. 30: 1255-1259 https://doi.org/10.2135/cropsci1990.0011183X003000060020x
  10. Inderjit, K., M. Dakshini, and C. L. Foy. 1999. Priciples and practices in plant ecology- allelochemical interations. CRC Press, Boca Raton, FL, USA.
  11. Jennings J. A. 1996. Rotational interval, soil texture, and zone of influence studies on alfalfa autotoxicity. Ph.D. Diss. University of Missouri, Columbia, MO, USA.
  12. Jensen, E. H. 1984. Problems of continuous alfalfa. In Proc. Western Nevada Alfalfa Symposium, Nevada Coop. Ext. Publ. 165, Fallon, NV, USA.
  13. Jensen, E. H., B. J. Hartman, F. Lundin, S. Knapp, and B. Brookerd. 1981. Autotoxicity of alfalfa. Max C. Fleischmann College of Agric., Univ. of Nevada Agric. Exp. Stn. Bull. R 144
  14. Macias, F. A., J. C. G. Galindo, J. M. G. Molinillo, and H. G. Cutler. 2003. Allelopathy Chemistry and mode of action of allelochemicals. CRC Press, Boca Raton, FL, USA.
  15. Miller, D. A. 1983. Allelopathic effects of alfalfa. J. Chem. Ecol. 9 : 1059-1071 https://doi.org/10.1007/BF00982211
  16. Miller, D. A. 1996. Allelopathy in forage crop systems. Agron. J. 88 : 854-859. https://doi.org/10.2134/agronj1996.00021962003600060003x
  17. Molisch, H. 1937. Der Einfluss einer Pflanze auf die andere -Allelopathie. Fischer. Jena
  18. Putnam, A. R. 1985. Allelopathic research in agriculture: Past highlights and potential. p. 1-8. In A. C. Thompson (ed) The Chemistry of Allelopathy. Am. Chern. Soc., Washington DC, USA.
  19. Rice, E. L. 1984. Allelopathy. 2nd edn. Academic Press, NY, USA.
  20. Rose, S. J., O.c Burnside, J. E. Spechts, and B. A. Swisherd. 1984. Competition and allelopathy between soybeans and weeds. Agron. J 76 : 523-528 https://doi.org/10.2134/agronj1984.00021962007600040005x
  21. Stowe, L. G. and A. Osborn. 1980.The influence of nitrogen and phosphorous levels on the phytotoxicity of phenolic compounds. Can. J. Bot. 58: 1149-1153 https://doi.org/10.1139/b80-142
  22. Tesar, M. B. 1993. Delayed seeding of alfalfa avoids autotoxicity after plowing or glyphosate treatment of established stands. Agron. J. 85: 256-263 https://doi.org/10.2134/agronj1993.00021962008500020018x
  23. Whitehead, D. C. 1964. Identification of p-hydroxybenzoic, vanillic, p-coumaric and ferulic acids in soils. Nature 202: 417-418. https://doi.org/10.1038/202417a0