• Ocean and Polar Research
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• v.29 no.4
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• pp.349-366
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• 2007

Seasonal variations of various physicochemical components (temperature, salinity, pH, DO, COD, DOC, nutrients-silicate, DIN, DIP) and potential limiting factor for phytoplankton primary production were studied in the surface water of semi-enclosed Masan Bay. Seasonal variations of nutrient concentrations, with lower values in summer and winter, and higher in fall, are probably controlled by freshwater loadings to the bay, benthic flux and magnitude of occurrence of phytoplankton communities. Their spatial distributional patterns are primarily dependent on physical mixing process between freshwater and coastal seawater, which result in a decreasing spatial gradient from inner to outer part of the bay. In the fall season of strong wave action, the major part of nutrient inputs (silicate, ammonium, dissolved inorganic phosphorus) comes from regeneration (benthic flux) at sediment-water interface. During the summer period, high Si:DIN and Si:DIP and low DIN:DIP relative to Redfield ratios suggest a N- and secondarily P-deficiency. During other seasons, however, silicate is the potential limiting factor for primary production, although the Si-deficiency is less pronounced in the outer region of the bay. Indeed, phytoplankton communities in Masan Bay are largely affected by the seasonal variability of limiting nutrients. On the other hand, the severe depletion of DIN (relatively higher silicate level) during summer with high freshwater discharge probably can be explained by N-uptake of temporary nanoflagellate blooms, which responds rapidly to pulsed nutrient loading events. In Masan Bay, this rapid nutrient consumption is considerably important as it can modify the phytoplankton community structures.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1841-1847
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• 2008

Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for the V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1 g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium, using the rpoS gene in pure cultures and in infected oyster tissues.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.1
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• pp.47-58
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• 1994

Field observation and numerical experiments with a two-dimensional depth-integrated model were undertaken in order to investigate some of the effects on the flow structure resulting from the construction of a bridge connecting Kohung Peninsula and the Narodo Islands on the southern coast of Korea. Tidal currents passing through the straits between the Narodo Islands showed that, although the phase lagged one hour behind that passing through the strait between Kohung Peninsula and Naenarodo Island, it still kept strong flows of more than 80cm/sec near the bottom. The seawater temperature and salinity within the study area seemed to be higher southward but uniform vertically. The results of the drogue experiments in the straits between the Narodo Islands showed that the drogues moved northward of Sayangdo in the early part of the flood tide, but southward in the late part of the flood tide and finally stopped a mile from the east coast of Surakdo. On the other hand, the numerical computation showed that the flow structures after construction of the bridge piers were basically in line with those before construction of the bridge piers, except for the slight variations of velocities in the vicinity of the bridge piers. A large scale clockwise circulation has been confirmed in the south area of Namsungri of Kohung Peninsula from the computational results of tide-induced residual currents. Referring to these computational results, the impact category on the flow structures due to the bridge piers construction has been estimated to be within around 2km. The results were in good agreement with the field observations.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.3
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• pp.129-138
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• 1978

The contents of inorganic conservative constituents in the downstream water were determined in spring tides of every month from May 1977 to April 1978 at eight stations of Nagdong River. Samples were taken at the intervals of one or two hours from 7 a. m. to 7 p. m. at each station. Annual ranges and means of the chemical constituents over all the stations except station one, Kupo, ate as follows: pH 6.4-9.3, 7.7; electrical conductivity $0.085-0.345\times10^3\;\mu\mho/cm,\;0.196\times10^3\;\mu\mho/cm;$ chloride 5.8-50.0ppm, 17.7ppm: fluoride ND-0.19 ppm, 0.06 ppm: sulfate 5.5-41.1 ppm, 20.7 ppm; calcium 6-26 ppm, 17 ppm; magnesium 2.0-12.8 ppm, 5.1 ppm; sodium 7-26 ppm, 13 ppm; potassium 1.4-3.8 pprn, 2.3 ppm respectively. The seasonal variations of contents of the chemical constituents were not large and showed nearly definite values at all the stations except station one, Kupo. At station one, seasonal variations were large and the contents were excessively high due to inflow of seawater compared with other stations. The values over 50 ppm in chloride were not determined during the determination period at Mul Geum where the intake station for Busan city water is located. Most constituents except pH and fluoride were over the criteria for drinking water at Kupo, while at other stations only pH value was exceeded the upper limit of the criterion especially in summer period. The pH values tended to increase in the afternoon when water temperature was high. The chloride concentration was shown the highest value at station one, Kupo, with about 2 hours delay after high water of Busan harbour and 3-3.5 hours at Mul Geum.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.1065-1068
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• 2008

Marine bridges are readily deteriorated due to the exposure to marine environment. The concrete deterioration occurred by corrosion of steel in concrete is mainly relevant to chloride in seawater. Chloride ions penetrate through porous concrete, and then reach to the reinforcing steel, and finally corroded them. The corrosion by-products(rusts) increase the volume as much as 6 to 10 times of origin steel. this creates expanding pressure and tensile stress, which cause the structures cracking and spalling. Sometimes the rebar corrosion is accelerated, and then collapsed catastrophically. In order to prevent corrosion damage, it is important to understand well regarding the reason of concrete corrosion, the quantification of its damage, and protection method/system to stop or to mitigate the corrosion. In this study, slab specimens were fabricated to evaluate the effect of cathodic protection which was simulated to marine bridges, and/or port structures. Zn-mesh sacrificial anode has been applied as a chathodic protection system and accelerated test conditions, i.e. temperature and salt concentration have been used in this study.

• Proceedings of the SAREK Conference
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• 2008.11a
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• pp.611-617
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• 2008

Nowadays Plate Heat Exchanger (PHE) is widely used in different industries such as chemical, food and pharmaceutical process and refrigeration due to the efficient heat transfer performance, extreme compact design and efficient use of the construction material. In present study, discussed main conception of plate heat exchanger and applied in vacuum. PHE and aimed apply in the fresh water generator which installed in ship to desalinate seawater to fresh water use heat from engines. The experiment is proceeded to investigate the heat transfer between cold and hot fluid stream at different flow rate and supply temperature of hot fluid. Generated fresh water as outcome of the system. PHE is an important part of a condensing or evaporating system. One of common assumptions in basic heat exchanger design theory is that fluid is to be distributed uniformly at the inlet of each fluid side and throughout the core. However, in practice, flow mal-distribution is more common and can significantly reduce the heat exchanger performance. The flow and heat transfer are simulated by the k-$\varepsilon$ standard turbulence model. Moreover, the simulation contacted flow maldistribution in a PHE with 6 channels.

• Solar Energy
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• v.17 no.4
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• pp.13-22
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• 1997

Drag reduction produced by the dilute solution of polymer under turbulent flow in a rotating disk apparatus(RDA) was investigated in this study for the purpose of potential application to the Ocean Thermal Energy Conversion(OTEC) system. Four different molecular weights of poly(ethylene oxide)(PEO) were used as drag reducing additives, and synthetic seawater was adopted as a solvent. Experiments were undertaken to observe the dependence of drag reduction on various factors such as polymer molecular weight, polymer concentration and the rotating speed of the disk. The concentration dependence on the drag reduction of this polymer system was shown to obey an empirical drag reduction equation of the Virk's universal correlation.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.5
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• pp.538-544
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• 2013

In this study, cavitation characteristics of a thermal spray coating were evaluated in order to improve durability and cavitation resistance. For a coating material, a Ni-based self-fluxing alloy was thermal-sprayed over a ALBC3 alloy substrate and subsequently modified by heat treatment.The resulted self-fluxing coating layer had relatively high hardness compared to the base material, and thus would be expected to exhibit good durability. However, the cavitation characteristics were deteriorated due to the intrinsic porous structure of the coating. Therefore, it is essential to optimize heat treatment condition during thermal spraying coating process for self-fluxing alloy, and in this research the increase in heat treatment temperature is thought to increase the fluidity of B and Si in the self-fluxing alloy and to remove pores or defects, leading to the characteristics enhancement.

• Journal of Marine Bioscience and Biotechnology
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• v.6 no.2
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• pp.118-122
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• 2014

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

• Journal of Microbiology and Biotechnology
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• v.22 no.10
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• pp.1412-1422
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• 2012

The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase, isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran, ammonium chloride, and initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for production of CMCase were 79.9 g/l, 8.52 g/l, and 6.1. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for production of CMCase were 3.72, 0.54, 0.70, and 0.34 g/l. The optimal temperature for cell growth and the CMCase production by C. lytica LBH-14 were $35^{\circ}C$ and $25^{\circ}C$, respectively. The maximal production of CMCase under optimized condition for 3 days was 110.8 U/ml, which was 5.3 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of CMCase by C. lytica LBH-14. The time for production of CMCase by a newly isolated marine bacterium with submerged fermentations reduced to 3 days, which resulted in enhanced productivity of CMCase and a decrease in its production cost.