• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.11 no.4
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• pp.165-171
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• 2006

This is the research to prepare a purification program for relatively less polluted stream by using phytoremediation. We calculated a treatment amount of nutrients followed by growth of Helianthus annuus (a kind of sunflower), setting up the plant reactor in the hothouse. Moreover, to investigate a field applicability, we could find increased contents of nitrogen, carbon and hydrogen in plants by setting up a H. annuus planted artificial floating island in an irrigation canal. When we changed the dissolved inorganic nitrogen(DIN) concentration of the influent from 28.5 to 199.2 mg/l and the dissolved inorganic phosphorus(DIP) concentration of the influent from 13.3 to 25.4 mg/l, growth disorder has not appeared though it is much higher than the criterion of water for irrigation. In this case, the removal rate of DIN was $81.7\sim98.6%$, and that of DIP was $81.9\sim98.4%$ in 3 days stay on average. It has appeared that the efficient hydraulic retention time(HRT) was 48 hours. The following contents of nitrogen, carbon and hydrogen of H. annuus appeared in the artificial floating island: nitrogen was $3.2\sim7.8%$ in the trunk and $3.0\sim6.3%$ in the root. Carbon was $40.1\sim57.7%$ in the trunk and $43.4\sim53.8%$ in the root.

• Development and Reproduction
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• v.15 no.4
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• pp.365-371
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• 2011

Water temperature governs various biological events in many aquatic animals including fish. Temperature changes the rates of gametogenesis and development, in some cases, is even capable of reversing fish sex. Treatments of fish with unusually high temperature are known to induce the expression of HSP70 gene. Development of an effective inhibitor for HSP70 gene expression is, thus, crucial to study the role of HSP70 in the temperature sensitive biological events. We have investigated the inhibitory effect of quercetin, 3,3',4',5,7-pentahydroxyflavon, a natural flavonid, on the expressions of HSP70 gene induced by high temperature ($36^{\circ}C$) in the Nile tilapia, Oreochromis niloticus, larvae and juveniles (10~13 cm in total length). The expression of HSP 70 gene was significantly decreased in tilapia larvae immersed in 50 ${\mu}M$ or 100 ${\mu}M$ quercetin solution for 6 hours before the exposure to high temperature (P<0.05). In particular, the level of HSP70 expression in fish treated with 100 ${\mu}M$ was as low as that of fish without high temperature treatment. Juveniles of tilapia were individually injected with 0.1 $m{\ell}$ of either 0.5 mM, 5 mM or 20 mM of quercetin solution before the exposure to high temperature. As the results, the expression of HSP70 gene in the gonad and brain of juvenile fish was significantly inhibited by the injection of 0.5 mM quercetin solution (P<0.05), but not by higher concentrations. We report, for the first time in the fish, that quercetin effectively inhibits the expression of HSP70 gene induced by high temperature and 100 ${\mu}M$ for the immersion of larvae and 0.5 mM for the injection to juvenile can be used for the effective concentrations for the study of temperature sensitive biological events in tilapia.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.393-400
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• 2011

This study investigates the effects of a hangover beverage (MIX) that contains minerals (highly-salty mineral water, HSMW) and several medicinal plant extracts, on antioxidant and alcohol-metabolizing enzymes in alcohol administered Sprague-Dawley rats. HSMW is pumped from below the sedimentary rock layer of Dadaepo, Busan, South Korea, which is 1,050 m below the land surface; it tastes salty, like sea water. In terms of medicinal plant extracts, the total phenolic and flavonoid contents of Rubus coreanus and Cornus officinalis were measured as being significantly higher than those in Curcuma longa. The results suggest that treatment with MIX significantly increases superoxide dismutase (SOD) activity and DPPH radical scavenging activity. In the 10% HSMW-, for MIX- and company product (CP)-treated groups, the concentration of blood alcohol was significantly reduced 1~5 hr after alcohol loading, compared to that in the control group. In hepatic alcohol-metabolizing enzyme activities, alcohol dehydrogenase (ADH) activity was found to be higher in the MIX- and CP-treated groups than in controls, whereas acetaldehyde dehydrogenase (ALDH) activity was significantly higher in the CP-treated groups than other groups. This study concludes, therefore, that MIX (HSMW) minerals, like as Zn, Ca, Mg, Mn, and others stimulate alcohol-metabolizing enzymes, while the antioxidants of plant extracts prevent the damage otherwise incurred by alcohol toxicity. These results suggest that the hangover beverage (MIX) alleviates alcohol hangover symptoms by stimulating activities related to hepatic alcohol-metabolizing enzymes and antioxidant effects.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.29 no.1
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• pp.28-41
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• 2024

The carbon balance serves as a valuable indicator of a plant's physiological status under diverse environmental conditions. We investigated the photosynthetic and respiratory responses of the Asian surfgrass Phyllospadix iwatensis along the northeast coast of the Korean peninsula in response to changing water temperature (ranging from 5℃ to 30℃) to estimate the seasonal whole-plant carbon balance through a series of incubation experiments. The maximum gross photosynthetic rate (P_max) showed a significant difference among the temperature treatments, while there was no significant difference in photosynthetic efficiency (α). The maximum gross photosynthetic rate of P. iwatensis reached its peaks at 20℃ treatment (101.65 μmol O₂ g^-1 DW h^-1) but decreased rapidly at 30℃. The saturation irradiance (I_k), compensation irradiance (I_c), and respiration rate (R) of P. iwatensis exhibited significant differences among the temperature treatments. The saturation irradiance increased up to 20-25℃ (121.59-124.50 μmol photons m^-2 s^-1) and sharply decreased at 30℃. The compensation irradiance and respiration rate increased steadily with rising water temperature. The ratio of P_max to R (P_max:R ratio) was the highest at 5℃ but dramatically decreased at 30℃. The whole-plant carbon balance, calculated based on photosynthetic parameters, respiration rates, and biomass, exhibited distinct seasonal variation, increasing during winter and spring and decreasing during summer and fall, which is consistent with the highest in situ growth in spring and severely limited growth at the highest water temperature conditions. Phyllospadix iwatensis displayed a negative carbon balance during late summer, fall, and winter, but demonstrated a positive carbon balance during spring and early summer. Our findings suggest that the rising seawater temperatures associated with climate change may lead to significant alterations in the seagrass ecosystem functioning along the rocky shores of the Korean east coast.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.4
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• pp.363-372
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• 2019

The leaves and stems of Centella asiatica have a long history of their usage as a medicine for the treatment of skin diseases such as ulcers and psoriasis, especially in Asia. Triterpenoids, the active components of Centella asiatica including asiaticoside, madecasosside, asiatic acid and madecassic acid, have shown to inhibit skin inflammation as well as improve skin photoaging. The main objective of this study is to investigate whether the Centella asiatica ripened with lava seawater which is rich in minerals known to be beneficial to human body can provide anti-inflammatory and moisturizing effects to skin. HPLC analysis showed that the concentration of triterpenoids increased further after ripening Centella asiatica with lava seawater. In order to confirm the inflammatory efficacy of the extract of the extract of the ripened Centella asiatica, the production of NO in LPS-activated RAW 264.7 cells and the expression of inflammatory cytokines in PM10 or UVB-induced HaCaT cells were observed. We found that the extract of the ripened Centella asiatica inhibited the expression of NO, IL-6, IL-8, and TNF-a and had higher inhibitory effect compared to the extract of the non-ripened Centella asiatica. In order to confirm the skin moisturizing effect, we investigated the synthesis of HA in HaCaT cells. The result showed HA production was enhanced in a concentration-dependent manner from the ripened group, while there was no efficacy from the non-ripened group. Taken together, it is concluded that the extract of the Centella asiatica ripened with lava seawater was effective in anti-inflammation and moisturization.

• Korean Journal of Microbiology
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• v.51 no.2
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• pp.126-132
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• 2015

Three different methods (simple washing of plastic and pulp sample, washing after direct heating of the diapers, and the heating after washing of plastic and pulp sample) were carried out to decrease total coliforms and heterotrophic plate count (HPC) in the diaper's plastic and pulp. Plastic and pulp samples were obtained from diaper by treatment with 10% $CaCl_2$ and 4% sea salt water, dilution with 1,000 ml tap water, and draining by using sieves. Three times washing was the most appropriate for the reduction of microorganisms in plastic and pulp. By three times washing, the number of total coliforms in the plastic and pulp samples showed 92.8% and 99.8% of decrease, respectively, and the number of HPC showed 97.3% of decrease in the plastic and 98.5% of decrease in the pulp. The washing after direct heating of the fecal contaminated diapers was not effective because HPC in the plastic and pulp samples were still detected about 2-3 log CFU/g in the plastic and 1-2 log CFU/g in the pulp, respectively, even after heating at $60^{\circ}C$, $80^{\circ}C$, $100^{\circ}C$ for 12 h. Meanwhile, total coliforms and HPC were completely sterilized at $80^{\circ}C$ for 4 h by heating after washing of plastic and pulp samples, suggesting that this method was the most appropriate method for the reduction of microorganisms in plastic and pulp obtained from fecal contaminated diapers.

• 한국해양학회지
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• v.10 no.1
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• pp.33-40
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• 1975

The ion exchange sorption and elution behavior of toxic heavy metal ions, such as Hg(II) and Zn(II), have been studied in aqueous and methanolic media of MCl (M: K, Na and NH$\_$4/). The ion exchange resins studied are Dowex 1-X8, Cl$\^$-/ (50-100 or 200-400 mesh) and Dowex 50W-X8, M$\^$+/ form (M: K, Na, NH$\_$4/ and H). the sorption and elution of metal ion on the resin is largely due to the formation of the anionic chlororocomplex of metal ion. The addition of methanol in the medium contributes markedly to the distribution data. In order to apply this work for the treatment of polluted sea water with toxic heavy metal ions, removal experiment of the metal ions from the synthetic sample solution was investigated.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.spc
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• pp.30-36
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• 2022

A sensor is needed to continuously and automatically measure the change in HNS concentration in industrial facilities that directly discharge to the sea after water treatment. The basic function of the sensor is to be able to detect ppb levels even at room temperature. Therefore, a method for increasing the sensitivity of the existing sensor is proposed. First, a method for increasing the conductivity of a film using a conductive carbon-based additive in a nanoparticle thin film and a method for increasing ion adsorption on the surface using a catalyst metal were studied.. To improve conductivity, carbon black was selected as an additive in the film using ITO nanoparticles, and the performance change of the sensor according to the content of the additive was observed. As a result, the change in resistance and response time due to the increase in conductivity at a CB content of 5 wt% could be observed, and notably, the lower limit of detection was lowered to about 250 ppb in an experiment with organic solvents. In addition, to increase the degree of ion adsorption in the liquid, an experiment was conducted using a sample in which a surface catalyst layer was formed by sputtering Au. Notably, the response of the sensor increased by more than 20% and the average lower limit of detection was lowered to 61 ppm. This result confirmed that the chemical resistance sensor using metal oxide nanoparticles could detect HNS of several tens of ppb even at room temperature.