• 제목/요약/키워드: screening conditions

검색결과 601건 처리시간 0.026초

한국인의 평생건강관리 프로그램 (Lifetime Health Maintenance Program for Korean)

  • 김영식
    • 한국건강관리협회지
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    • 제2권3호
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    • pp.97-116
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    • 2004
  • Lifetime Health Maintenance Program(LHMP) for Koreans is the Korean guide to clinical preventive services which include screening for chronic diseases, counseling and immunization. The Korean Task Force on the LHMP vigorously reviews evidence for interventions to prevent over 50 different illnesses and conditions which are important in Korea. The problems addressed in this program are common ones seen every day by primary care physicians in Korea: cardiovascular, infectious diseases, gastrointestinal and endocrine diseases, cancers, alcohol and smoking, and many others. Primary care clinicians have a key role in screening for many of these problems and immunizing against others. Of equal importance, however, is the clinician's role in counseling patients to change unhealthful behaviors related to diet, smoking, exercise, injuries, and sexually transmitted diseases. The recommendations are grouped by age, sex, and other risk factors.

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우울증 환자들에서 불안장애의 인지 (Recognition of Anxiety Disorders in Depressed Patients)

  • 김정범;정성원
    • 대한불안의학회지
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    • 제3권1호
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    • pp.15-19
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    • 2007
  • Substantial rates of comorbid anxiety disorders have been found using structured research diagnostic interviews in depressed outpatients. Comorbid anxiety disorders is underrecognized in routine clinical settings, in which clinicians usually use unstructured diagnostic interviews. Recognition of comorbid conditions such as anxiety disorders in patients seeking treatment for depression is clinically important because the presence of these disorders might influence selection of treatment options or predict the chronicity of the depression. A reliable and valid self-report screening questionnaire, such as the Psychiatric Diagnostic Screening Questionnaire (PDSQ), would potentially enhance usual clinical practice as a useful diagnostic aid.

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베타-1,3-글루칸 합성효소 저해제의 스크리닝을 위한 효소원 제조법 (Preparation of Enzyme Source for Screening of Enzyme Inhibitor of $\beta$-1,3-glucan Synthase)

  • 박희문;이동원;송미령;김정윤;김성욱;복성해
    • 한국미생물·생명공학회지
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    • 제23권3호
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    • pp.311-315
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    • 1995
  • Assay conditions for screening of $\beta$-1,3-glucan synthase inhibitor were evaluated. Cells in the beginning of mid-log phase showed the highest activity of the $\beta$-1,3-glucan synthase. Cells permeabilized with 1% digitonin treatment could be used as a good crude enzyme source for convenient screening of the $\beta$-1,3-glucan synthase inhibitors. Calcofluor white (0.125% in final) and papulacandin B (25 $\mu$g/ml) inhibit 90% and more than 50% of the $\beta$-1,3-glucan synthase activity, respectively. Cells grown at 37$\circ$C showed higher enzyme activity than those of 25$\circ$C. Catalytic factor of the $\beta$-1,3-glucan synthase was solubilized from particulated membrane preparations, holoenzyme, by extracting with 0.00938% CHAPS.

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Isolation and Characterization of Soil Strains Producing Glutaryl-7-Aminocephalosporanic Acid Acylase

  • Knang, Yong-Ho;Yoo, Ryong-Hoon
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제2권2호
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    • pp.105-108
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    • 1997
  • A search was undertaken to screen microorganisms that produce an enzyme capable of deacylating glutary1-7-amincephalosporanic acid to 7-aminocephalosporanic acid in soil samples. The screening was carried out by preparing enrichment cultures containing glutary-7ACA and cephalosporin C as selective carbon sources. A non-${\beta}$-lactam model compound,, glutary-p-nitroanilide, was synthesized as a substrate suitable for the rapid screening of microorganisms isolated from the enrichment cultures. Two isolates exhibiting acylase activity, designated BY7.4 and BY8.1, were identified as strains of Pseudomonas species. Pseudomonas BY8.1 showed higher acylase activity toward G1-7ACA than Pseudomonas BY7.4. Environmental conditions for the optimal acylase activity of Pseudomonas BY8.1 were shown to be pH9 and 30$^{\circ}C$.

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유전성 대사 질환의 분자 유전학적 진단 (Molecular Genetic Diagnosis of Inherited Metabolic Diseases)

  • 기창석;이수연;김종원
    • 대한유전성대사질환학회지
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    • 제5권1호
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    • pp.108-115
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    • 2005
  • Inherited metabolic diseases (IMD) comprise a large class of genetic diseases involving disorders of metabolism. The majorities are due to defects of single genes that code for enzymes that facilitate conversion of various substances into others. Because of the multiplicity of conditions, many different diagnostic tests are used for screening of IMD. Molecular genetic diagnosis is the detection of pathogenic mutations in DNA and/or RNA samples and is becoming a much more common practice in medicine today. The purpose of molecular genetic testing in IMD includes diagnostic testing, pre-symptomatic testing, carrier screening, prenatal diagnosis, preimplantation testing, and population screening. However, because of the complexity, difficulty in interpreting the result, and the ethical considerations, an understanding of technical, conceptual, and practical aspects of molecular genetic diagnosis is mandatory.

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한국인의 평생건강관리 프로그램 (Lifetime Health Maintennance Program for Korean)

  • 김영식
    • 한국건강관리협회지
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    • 제2권2호
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    • pp.231-245
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    • 2004
  • Lifetime Health Maintenance Program(LHMP) or Koreans is the Korean guide to clinical preventive services which include screening for chronic diseases, counseling and immunization. The Korean Task Force on the LHMP vigorously reviews evidence for interventions to prevent over 50 different illnesses and conditions which are important in Korea. The problems addressed in this program are common ones seen every day by primary care physicians in Korea: cardiovascular, infectious diseases, gastrointestinal and endocrine diseases, cancers, alcohol and smoking, and many others. Primary care clinicians have a key role in screening for many of these problems and imunizing against others Of equal importance, however, is the clinician's role in counseling patients to change unhealthful behaviors related to diet, smoking, exercise, injuries, and sexually transmitted diseases The recommendations are grouped by age, sex, and other risk factors.

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Screening of Genes Related to Methylglyoxal Susceptibility

  • Kim, In-Sook;Kim, Joon-Ho;Min, Bum-Chan;Lee, Chang-Han;Park, Chan-Kyu
    • Journal of Microbiology
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    • 제45권4호
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    • pp.339-343
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    • 2007
  • Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.

HPLC On-line $ABTS^{+}$ Antioxidant screening 시스템을 이용한 한국산 녹차로부터 Catechin compounds의 황산화 활성분석 (Antioxidant activity analysis of Catechin compounds in Korean green tea using HPLC On-line $ABTS^{+}$ Antioxidant screening system)

  • 이광진
    • KSBB Journal
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    • 제23권1호
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    • pp.96-100
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    • 2008
  • 한국산 녹차로부터 HPLC On-line $ABTS^{+}$ screening기법을 사용하여 catechin compounds의 특성중의 하나인 항산화활성을 빠르게 분석하였으며 녹차로부터 catechin compound의 추출을 다양한 온도와 시간의 추출방법을 적용하였다. 전 처리한 추출액에 포함된 catechin compounds을 분석하고 최적의 추출조건을 실험적으로 모색하였다. 실험결과에 의하면 추출온도 $60^{\circ}C$, 추출시간 3분으로 추출한 시료가 항산화활성이 가장 우수하였다.

A Simple and Rapid Methicillin-Resistant Staphylococcus aureus (MRSA) Screening Test Using a Mannose-Binding Lectin (MBL)-Conjugated Gold Nanoparticle Probe

  • So Yeon Yi;Jinyoung Jeong;Wang Sik Lee;Jungsun Kwon;Kyungah Yoon;Kyoungsook Park
    • Journal of Microbiology and Biotechnology
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    • 제33권5호
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    • pp.698-705
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    • 2023
  • Rapid diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple and rapid MRSA screening test based on the aggregation effect of mannose-binding lectin (MBL)-conjugated gold nanoparticles (AuNP), called the MRSA probe. Recombinant MBL protein is a member of the lectin family and part of the innate immune system. It can recognize wall teichoic acid (WTA) on the membrane of MRSA more specifically than that of methicillin-sensitive Staphylococcus aureus (MSSA) under optimized salt conditions. Thus, the MRSA probe can selectively bind to MRSA, and the aggregation of the probes on the surface of the target bacteria can be detected and analyzed by the naked eye within 5 min. To demonstrate the suitability of the method for real-world application, we tested 40 clinical S. aureus isolates (including 20 MRSA specimens) and recorded a sensitivity of 100%. In conclusion, the MRSA probe-based screening test with its excellent sensitivity has the potential for successful application in the microbiology laboratory.

Usefulness of four commonly used neuropathic pain screening questionnaires in patients with chronic low back pain: a cross-sectional study

  • Gudala, Kapil;Ghai, Babita;Bansal, Dipika
    • The Korean Journal of Pain
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    • 제30권1호
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    • pp.51-58
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    • 2017
  • Background: Recently symptoms-based screening questionnaires have gained attention for screening for a neuropathic pain component (NePC) in various chronic pain conditions. The present study assessed the usefulness of four commonly used NePC screening questionnaires including the Self-completed douleur neuropathique 4 (S-DN4), the ID Pain, the painDETECT questionnaire (PDQ), and the Self-completed Leeds Assessment of neuropathic Symptoms and Signs (S-LANSS) questionnaire in patients with chronic low back pain (CLBP) to assess the presence of NePC. Methods: This is a single-center cross-sectional study where patients with CLBP, with or without leg pain, were included. Participants were initially screened for NePC presence by a physician according to the regular practice, and later assessed using screening questionnaires. The diagnostic accuracy of these questionnaires was compared assuming the physician-made diagnosis as the gold standard. Results: A total of 215 patients with CLBP of which 164 (76.3%, 95% CI, 70.2-81.5) had a NePC were included. S-DN4, ID Pain, and PDQ have an area under the curve (AUC) > 0.8 indicating excellent discrimination. However, S-LANSS has an AUC of 0.69 (0.62-0.75), indicating low discrimination. S-DN4 has a significantly higher AUC as compared to ID Pain (d(AUC) = 0.063, P < 0.01) and S-LANSS (d(AUC) = 0.197, P < 0.01). But the AUC of S-DN4 does not significantly differ from that of PDQ (d(AUC) = 0.013, P = 0.62). Conclusions: S-DN4, ID Pain, and PDQ, but not S-LANSS, have good discriminant validity to screen for NePCs in patients with CLBP. Despite using all the tests, 20-30% of patients with an NePC were missed. Thus, these questionnaires can only be used as an initial clue in screening for NePCs, but do not replace clinical judgment.