• Title/Summary/Keyword: scrP

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Characterization of the scr Gene Cluster Involved! in Sucrose Utilization in Bifidobacterium longum (Bifidobacterium longum의 Sucrose 대사 관련 scr 유전자군의 특성 규명)

  • 권태연;이종훈
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.199-205
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    • 2004
  • The nucleotide sequence of 8.6-kb EcoRI fragment containing sucrose phosphorylase gene isolated from Bifidobacterium longum SJ32 was determined. It was found that the fragment contained five open reading frames including the gene cluster for sucrose utilization such as a sucrose phosphorylase (ScrP), a sucrose transporter (ScrT), and a GalR-LacI-type transcriptional regulator (ScrR) identified by amino acid homology. Each gene showed over 94% amino acid homology among various B. longum strains. Genomic organization of the gene cluster is the same as those of other strains of B. longum but different from that of B. lactis. In spite of high homology of each gene among B. longum strains, the difference of flanking sequences of the gene cluster between strains SJ32 and NCC2705 insinuates the horizontal transfer of scrPTR between B. longum strains. The increase of sucrose phosphorylase activity in heterologous E. coli system by the co-expression of scrT with scrP against the single expression of scrP was measured. It seems to be the result of sucrose uptake increment by scrT in the host and is an indirect evidence that scrT is the gene for sucrose transport. The existence of multiple sucrose uptake systems in B. longum is supposed from the findings of several genes besides scrPTR involved in sucrose uptake in the genome of B. longum NCC2705.

Temperature Variation of Exhaust Gas in Diesel Generator for Low Pressure SCR (저압 SCR을 위한 디젤발전기 배기가스 온도 변화)

  • Hong, Chul Hyun;Lee, Chang Min;Lee, Sang Duk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.2
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    • pp.355-362
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    • 2021
  • To facilitate low-pressure selective catalyst reduction (L.P SCR), a high exhaust-gas temperature of a four-stroke diesel engine for a ship's generator is required. This study aimed at reducing the exhaust-gas temperature by adjusting the valve open-close timing and fuel injection timing to satisfy the operating conditions of L.P SCR and prevent accidents associated with the generator engine due to high temperature. To lower exhaust-gas temperature, the angle of the camshaft was adjusted and the shim of the fuel injection pump was added. As a result, the maximum explosion pressure increased and the average of the turbocharger outlet temperature dropped. Considering the heat loss from the turbocharger outlet to the SCR inlet, the operation condition for L.P SCR was satisfied with 290 ℃. The study demonstrates that safe operation of a diesel generator can be achieved by lowering the exhaust-gas temperature.

Bioconversion of Soybean Curd Residues into Functional Ingredients with Probiotics

  • Oh, Soo-Myung;Kim, Chan-Shick;Lee, Sam-Pin
    • Preventive Nutrition and Food Science
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    • v.9 no.2
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    • pp.138-143
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    • 2004
  • Soybean curd residues (SCR) obtained from hot and cold manufacturing processes were fermented by indigenous microorganisms, Lactobacillus rhamnosus LS and Bacillus firmus NA-l for 15 h at 37$^{\circ}C$. The pH, acidity, viable cell counts, and tyrosine content were evaluated in samples with variations in sugar, starter and type of SCR. The raw Doowon SCR (D-SCR, cold-processed) fermented by indigenous microorganism had a 0.9% acidity and 6.7 ${\times}$ 10$^{7}$ CFU/g viable cell counts, compared with the 0.11 % acidity and 6.7 ${\times}$ 10$^{6}$ CFU/g viable cell counts of raw fermented Pulmuwon SCR (P-SCR, hot-processed). After fermentation of raw P-SCR with 1 % glucose and 1 % L. rhamnosus LS starter, the viable cell counts, tyrosine content and acidity were 4.7 ${\times}$ 10$^{8}$ CFU/g, 16.3 mg% and 0.9%, respectively. In addition, the raw P-SCR fermented with Bacillus firmus NA-l as co-starter had a 0.45% acidity, 2.4 ${\times}$ 10$^{8}$ CFU/g lactic acid bacteria, and 3.3 ${\times}$ 10$^{6}$ CFU/g Bacillus sp. In particular, the tyrosine content was increased 5 fold. The drying of fermented SCR was completed by hot-air drying (5$0^{\circ}C$) within 12 h; the dried P-SCR and D-SCR had 1.8 ${\times}$ 10$^{7}$ CFU/g and 5.3 ${\times}$ 10$^{6}$ CFU/g viable cell counts, respectively. The concentrate of methanol extract from fermented D-SCR inhibited the initial cell growth of E. coli, Staphylococcus aureus and Pseudomonas aeruginosa in liquid culture.

The deactivation behavior of SCR catalyst by alkali and alkali earth metal (알칼리 및 알칼리 토금속에 의한 SCR 촉매 비활성 거동)

  • Han, Seungyun;Shin, Min-Chul;Lee, Heesoo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.26 no.6
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    • pp.238-242
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    • 2016
  • The effect of the alkali, alkali earth metal elements on selective catalytic reduction(SCR) catalyst deactivation behavior were investigated in terms of microstructure, surface area, pore volume and De-NOx test. Poisoned SCR catalyst were manufactured by injection of $K_2CO_3$, $Na_2CO_3$, $Ca(CH_3COO)_2{\cdot}H_2O$, $C_4H_6MgO_4{\cdot}4H_2O$, $H_3PO_4$ solutions in the new SCR catalyst at $350^{\circ}C$ for 6 hours. New and poisoned catalysts surface were similar. But specific surface area, pore volume decrease from Na, Mg, K, Ca, P compared to new SCR catalyst. Especially, Na poisoned catalyst surface area and pore size extremely decreased by $10.20m^2/g$, $0.061cm^2/g$. De-NOx test results of new and poisoned catalysts at $150{\sim}450^{\circ}C$ indicated that alkali metal (K, Na) poisoned SCR catalysts have the lowest De-NOx efficiency, alkali earth metal poisoned SCR catalysts (Ca, Mg) De-NOx efficiency are higher than alkali metal poisoned SCR catalysts. P poisoned SCR catalyst De-NOx efficiency is similar new SCR catalyst. It were considered that physical deactivation of SCR catalyst was affected by SCR catalyst surface area and pore volume change.

Design of SCR-Based ESD Protection Circuit for 3.3 V I/O and 20 V Power Clamp

  • Jung, Jin Woo;Koo, Yong Seo
    • ETRI Journal
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    • v.37 no.1
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    • pp.97-106
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    • 2015
  • In this paper, MOS-triggered silicon-controlled rectifier (SCR)-based electrostatic discharge (ESD) protection circuits for mobile application in 3.3 V I/O and SCR-based ESD protection circuits with floating N+/P+ diffusion regions for inverter and light-emitting diode driver applications in 20 V power clamps were designed. The breakdown voltage is induced by a grounded-gate NMOS (ggNMOS) in the MOS-triggered SCR-based ESD protection circuit for 3.3 V I/O. This lowers the breakdown voltage of the SCR by providing a trigger current to the P-well of the SCR. However, the operation resistance is increased compared to SCR, because additional diffusion regions increase the overall resistance of the protection circuit. To overcome this problem, the number of ggNMOS fingers was increased. The ESD protection circuit for the power clamp application at 20 V had a breakdown voltage of 23 V; the product of a high holding voltage by the N+/P+ floating diffusion region. The trigger voltage was improved by the partial insertion of a P-body to narrow the gap between the trigger and holding voltages. The ESD protection circuits for low- and high-voltage applications were designed using $0.18{\mu}m$ Bipolar-CMOS-DMOS technology, with $100{\mu}m$ width. Electrical characteristics and robustness are analyzed by a transmission line pulse measurement and an ESD pulse generator (ESS-6008).

Predicting the Progression of Chronic Renal Failure using Serum Creatinine factored for Height (소아 만성신부전의 진행 예측에 관한 연구)

  • Kim, Kyo-Sun;We, Harmon
    • Childhood Kidney Diseases
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    • v.4 no.2
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    • pp.144-153
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    • 2000
  • Purpose : Effects to predict tile progression of chronic renal failure (CRF) in children, using mathematical models based on transformations of serum creatinine (Scr) concentration, have failed. Error may be introduced by age-related variations in creatinine production rate. Height (Ht) is a reliable reference for creatinine production in children. Thus, Scr, factored for Ht, could provide a more accurate predictive model. We examined this hypothesis. Methods : The progression of of was detected in 63 children who proceeded to end-stage renal disease. Derivatives of Scr, including 1/Scr, log Scr & Ht/Scr, were defined fir the period Scr was between 2 and 5 mg/dl. Regression equation were used to predict the time, in months, to Scr > 10 mg/dl. The prediction error (PE) was defined as the predicted time minus actual time for each Scr transformation. Result : The PE for Ht/Scr was lower than the PE for either 1/Scr or log Scr (median: -0.01, -2.0 & +10.6 mos respectively; P<0.0001). For children with congenital renal diseases, the PE for Ht/Scr was also lower than for the other two transformations (median: -1.2, -3.2 & +8.2 mos respectively; P<0.0001). However, the PEs for children with glomerular diseases was not as clearly different (median: +0.9, +0.5 & +9.9 respectively). In children < 13 yrs, PE for Ht/Scr was tile lowest, while in older children, 1/Scr provided the lowest PE but not significantly different from that for Ht/Scr. The logarithmic transformation tended to predict a slower progression of CRF than actually occurred. Conclusion : Scr, floored for Ht, appears to be a useful model to predict the rate of progression of CRF, particularly in the prepubertal child with congenital renal disease.

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Production of Fibrinolytic Enzyme and Peptides from Alkaline Fermentation of Soybean Curd Residue by Bacillus firmus NA-1 (Bacillus firmus NA-1 균주를 이용한 비지로부터 혈전분해능효소 및 펩타이드 생산)

  • Oh, Soo-Myung;Seo, Ji-Hyun;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.904-909
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    • 2005
  • To produce functional food ingredient from the soybean curd residue (SCR), alkaline fermentation was performed with SCR from cold processed (D-SCR) or hot processed (P-SCR) tofu. The solid- state fermentation was performed by Bacillus firmus NA-1 at $42^{\circ}C$. The fermentation of heat-treated D- SCR resulted in higher production of peptides and fibrinolytic enzyme compared with D-SCR without heating. The P-SCR showed higher production of peptides, fibrinolytic enzyme, indicating alkaline pH after fermentation for 18 hr. When the moisture content of P-SCR was reduced to $60\%$, the production of peptides and fibrinolytic enzyme were enhanced. The P-SCR fortified with $10\%$ MFS (micronized full-fat soy flour) showed higher fibrinolytic enzyme activity and consistency index by fermentation of Bacillus firmus NA-1 Furthermore, the P- SCR fortified with $20\%$ MFS indicated relatively higher peptide content, fibrinolytic enzyme activity and enhanced flavor. By increasing the addition of MFS, the peptide content of fermented P-SCR was increased significantly, but fibrinolytic enzyme was slightly decreased.

The SCR-based ESD Protection Circuit with High Latch-up Immunity for Power Clamp (파워 클램프용 래치-업 면역 특성을 갖는 SCR 기반 ESD 보호회로)

  • Choi, Yong-Nam;Han, Jung-Woo;Nam, Jong-Ho;Kwak, Jae-Chang;Koo, Yong-Seo
    • Journal of IKEEE
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    • v.18 no.1
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    • pp.25-30
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    • 2014
  • In this paper, SCR(Silicon Controlled Rectifier)-based ESD(Electrostatic Discharge) protection circuit for power clamp is proposed. In order to improve latch-up immunity caused by low holding voltage of the conventional SCR, it is modified by inserting n+ floating region and n-well, and extending p+ cathode region in the p-well. The resulting ESD capability of our proposed ESD protection circuit reveals a high latch-up immunity due to the high holding voltage. It is verified that electrical characteristics of proposed ESD protection circuit by Synopsys TCAD simulation tool. According to the simulation results, the holding voltage is increased from 4.61 V to 8.75 V while trigger voltage is increased form 27.3 V to 32.71 V, respectively. Compared with the conventional SCR, the proposed ESD protection circuit has the high holding voltage with the same triggering voltage characteristic.

The novel SCR-based ESD Protection Circuit with High Holding Voltage Applied for Power Clamp (파워 클램프용 높은 홀딩전압을 갖는 사이리스터 기반 새로운 구조의 ESD 보호회로)

  • Lee, Byung-Seok;Kim, Jong-Min;Byeon, Joong-Hyeok;Park, Won-Suk;Koo, Yong-Seo
    • Journal of IKEEE
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    • v.17 no.2
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    • pp.208-213
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    • 2013
  • In this paper, we proposed the novel SCR-based ESD protection circuit with high holding voltage for power clamp. In order to increase the holding voltage, the floating p+ and n+ to n-well and p-well, respectively, in the conventional SCR. The resulting increase of the holding voltage of the our proposed ESD circuit enables the high latch-up immunity. The electrical characteristics including ESD robustness of the proposed ESD circuit have been simulated using Synopsys TCAD simulator. According to the simulation result, the proposed device has higher holding voltage of 4.98 V than that of the conventional SCR protection circuit. Moreover, it is confirmed that the device could have the holding voltage of maximum 13.26 V with the size variation of floated diffusion area.

A Study on SCR-based ESD Protection Circuit with High Holding Voltage and All-Direction Characteristics (높은 Holding Voltage 및 All-Direction 특성을 갖는 SCR 기반의 ESD 보호회로에 관한 연구)

  • Jin, Seung-Hoo;Do, Kyoung-Il;Woo, Je-Wook;Koo, Yong-Seo
    • Journal of IKEEE
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    • v.24 no.4
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    • pp.1156-1161
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    • 2020
  • In this paper, we propose a new ESD protection circuit with improved electrical characteristics through structural changes of the existing one-way SCR. The proposed ESD protection circuit has high holding voltage characteristics due to the inserted N+ floating and P+ floating regions, and thus the latch-up immunity characteristics are improved. In addition, structural change enables ESD discharge in four types of Zapping mode (PD, PS, ND, NS), and has superior area efficiency than unidirectional SCR. In addition, the P+ floating and N+ floating lengths corresponding to the base length of the parasitic bipolar transistor, and the distance between P+ floating and N+ floating were designated as design variables, and the high holding voltage was verified through Synopsys' TCAD Simulator.