• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.88-92
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• 2005

Aptamer is the single-stranded oligonucleotide which binds to various target molecules such as proteins, peptides, lipids and small organic molecules with high affinity and specificity. DNA aptamers specific for the $17{\beta}-estradiol$ were selected by SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random DNA library. These DNA aptamers have a high affinity to $17{\beta}-estradiol$ as an endocrine disrupting chemical. Nanowell and $200{\mu}m$ gold electrode were used as substrate for DNA aptamer immobilization and electrochemical analysis. Especially, nanowell gold electrode was fabricated by e-beam lithography. The size of single nanowell is 130nm and 40,000 nanowells were deposited on one gold electrode. The immobilization method was based on the interaction between the biotinylated aptamer and streptavidin deposited on gold electrode previously. Immobilization procedure was optimized by surface plasma resonance (SPR) and electrochemical analysis. After the immobilization of DNA aptamer on streptavidin modified gold electrode, $17{\beta}-estradiol$ solution was treated on aptamer immobilized gold electrode. The current of gold electrode was decreased by the binding of $17{\beta}-estradiol$ to DNA aptamer immobilized on gold electrode. However, in negative control experiments of 1-aminoanthraquinone and 2-methoxynaphthalene, the current was rarely decreased. And more sensitive data was obtained from nanowell gold electrode comparing with $200{\mu}m$ gold electrode.

• Journal of The Korean Association For Science Education
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• v.18 no.3
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• pp.347-355
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• 1998

This paper reports on a way of using cognitive conflict in order to bring about conceptual changes. Cognitive conflict occurs when there is a discrepancy in students mind between everyday events and the scientific concepts which are used to explain these events. In order to overcome this conflict auxiliary scientific hypotheses can be introduced, which makes the resolution easier. In this paper, we suggest a new model of conceptual change and a model of instruction named 'structured contrastive activity' which consists of three stages: contrastive discussion by introducing the auxiliary hypotheses for clarifying the cognitive conflicts of students, observing the phenomena in idealised contexts in order to increase the status of the scientific concepts, laboratory experiments which bridge the two contexts.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.1-1
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• 2003

It is clear that computers will play a key role in the biology of the future. Even now, it is virtually impossible to keep track of the key proteins, their names and associated gene names, physical constants(e.g. binding constants, reaction constants, etc.), and hewn physical and genetic interactions without computational assistance. In this sense, computers act as an auxiliary brain, allowing one to keep track of thousands of complex molecules and their interactions. With the advent of gene expression array technology, many experiments are simply impossible without this computer assistance. In the future, as we seek to integrate the reductionist description of life provided by genomic sequencing into complex and sophisticated models of living systems, computers will play an increasingly important role in both analyzing data and generating experimentally testable hypotheses. The future of bioinformatics is thus being driven by potent technological and scientific forces. On the technological side, new experimental technologies such as microarrays, protein arrays, high-throughput expression and three-dimensional structure determination prove rapidly increasing amounts of detailed experimental information on a genomic scale. On the computational side, faster computers, ubiquitous computing systems, high-speed networks provide a powerful but rapidly changing environment of potentially immense power. The challenges we face are enormous: How do we create stable data resources when both the science and computational technology change rapidly? How do integrate and synthesize information from many disparate subdisciplines, each with their own vocabulary and viewpoint? How do we 'liberate' the scientific literature so that it can be incorporated into electronic resources? How do we take advantage of advances in computing and networking to build the international infrastructure needed to support a complete understanding of biological systems. The seeds to the solutions of these problems exist, at least partially, today. These solutions emphasize ubiquitous high-speed computation, database interoperation, federation, and integration, and the development of research networks that capture scientific knowledge rather than just the ABCs of genomic sequence. 1 will discuss a number of these solutions, with examples from existing resources, as well as area where solutions do not currently exist with a view to defining what bioinformatics and biology will look like in the future.

• Journal of Science Education
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• v.41 no.3
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• pp.405-425
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• 2017

This study aimed to investigate the life and works of Robert Hooke, an ingenious scientist in the era of scientific revolution, and to give some implications of invention education for science education. The publications and critics of Robert Hooke were analyzed to find out the personal setbacks how he showed excellent performances across the fields of science. The research finding showed that he tried to make geometric and visualized reasoning based on the empirical phenomenon, had much interest in the devices and methods for measurement and observation in the experiment, and made technical devices by himself. The ingenuity of Robert Hooke could be revealed by the rich resources in his childhood, his talent of drawing for depiction, and his colleagues and teachers with favors of diverse fields of disciplines and empirical tradition. As well, it was likely that his monistic viewpoint between the reality and scientific theories, led himself to develop interesting instruments for scientific experiments. Thus, this study suggested some implications to combine invention education with science education.

• Journal of Conservation Science
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• v.36 no.1
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• pp.1-14
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• 2020

This study investigates the crystallinity distribution of ancient silk. Owing to the inherent multi-hierarchical structure of silk protein and the complicated structural changes that occur due to various burial environments, it is challenging but worthwhile to study ancient silk ageing behavior, which is based on the fact that ageing begins with a single fiber and then spreads to a whole fabric. Crystallinity was one of the most effective indicators found to reveal the ageing status of silk. Therefore, a synchrotron radiation-based X-ray diffraction(SR-XRD) method was employed to study the crystallinity distribution of single fibers of ancient silk unearthed from seven archaeological sites in China from historical periods including the warring states, Han dynasty, Song dynasty, and Ming dynasty. In comparison, the conventional X-ray diffraction method, which uses large amounts of samples, was also performed to determine the integral crystallinity of ancient silk. Thermal stability experiments by thermogravimetry(TG) as well as morphology observations by scanning electron microscopy(SEM) and optical microscopy(OM) all confirmed the deterioration of ancient silk. Moreover, the ageing mechanism of ancient silk was proposed with the assistance of an artificial ageing study. The results confirmed the effectiveness of SR-XRD as an ageing indicator, revealing the crystallinity distribution. This research could provide motivation to determine the deterioration status of ancient silk, and would also aid in explaining the fragility of ancient silk due to ageing.

• Journal of Korean Elementary Science Education
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• v.32 no.4
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• pp.415-422
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• 2013

The purpose of this study was to investigate the effects of scientific program for gifted elementary students using metacognition on learning-flow and lingual interaction. For the purpose, the two classes for elementary science of P Institute for Gifted Education located in Pusan were assigned to experimental and control groups, respectively. The experimental and controls groups received eight sessions applying a program for scientifically gifted students using metacognition and same program without using it, respectively. The learning-flow test and the lingual interaction observed among students and between teacher and students pre and post program were compared and analyzed. The results shows that there was no significant difference between pretest and posttest in experimental group, indicating that learning-flow is not a construct obtained by short-term using of metacognition. For the lingual interactions, however, the amounts of conversation and the ratio of high level conversation in experimental group were 1.6 and 1.5 times higher than those in control group. This suggests that the lingual interaction may be varied among classes even with same experiments, by instruction method. The lingual interaction is not active in control group where the experimental results may be obtained easily only with sincere attitude while, in experimental group, questions using metacognition and providing intellectual stimulation is continuously presented, leading to high level of lingual interaction, therefore it is considered that the development of scientific program for gifted elementary students using these advantages is needed.

• Journal of The Korean Association For Science Education
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• v.16 no.4
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• pp.365-375
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• 1996

Improving of scientific inquiring ability is the major goal of current science curriculum, and the 6th science curriculum. But science educators consider that the existing textbooks and teaching manuals are insufficient to achieve this goal. For science teachers at teaching site to guide students efficiently in research work, development of teaching-learning programs is urgently demanded. Hypothesis Verification Learning Model(HVLM) was applied to classroom situation to improve ability of scientific inquiry in experiment teaching of middle school biology. The effects of the model were analyzed to suggest some approach method to reach the goal of science education in this study. The major results of this study are as following: 1. The students and teachers responded positively on this new learning model. an students were willing to participate in biology experiment and they said that to know what was unknown to them while exchanging ideas and opinions through the discussion, It was hard for teachers to instruct at the first time and it took much time for them to arrange materials ready, but it turned to be easier as time went on. 2. In science process skills, there was no significant difference statistically by new leaning model. Only the formulating a generalization or model showed significant difference statistically between the two groups. 3. For scientific attitude, experimental group did not show significant difference statistically between the two groups, but the experimental group showed statistically more significant positiveness in all areas afterwards than before. 4. In science achievement test, there was significantly higher than the control group. It is also analyzed that they remember the experiments in courses and results they planned and performed by themselves longer than these guided by teachers.

• Journal of Gifted/Talented Education
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• v.16 no.1
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• pp.43-60
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• 2006

This study mainly intended to investigate the relation between scientific inquiry and several entrance exam results of the gifted in earth science gifted program. The scientific inquiry was tested with their experiment designs. Entrance exams included achievement scores, creativity scores, problem solving, and oral test of high ability students. Student entrance scores were used to judge the level of students' competency in science. Recently students' ability of 'doing science' as measured by their designing experiments has been highly appreciated as a criteria of scientifically gifted students. One of the well known tests was Diet Cola Test developed by Fowler(1990). We used it as a test to figure out students' experiment design ability. We selected 22 gifted students in earth science. We compared their entrance test scores and Diet Cola Test results. Based on the comparison results we proposed several guideline of science education program for high ability students.

• Convergence Security Journal
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• v.23 no.2
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• pp.85-94
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• 2023

In the face of the serious security situation with the increasing threat from North Korea, Korean Army is pursuing a reduction in troops through the performance improvement project of the GOP science-based border security system, which utilizes advanced technology. In order for the GOP science-based border security system to be an effective alternative to the decrease in military resources due to the population decline, it must guarantee a high detection and identification rate and minimize troop intervention by dramatically improving the false detection rate. Recently introduced in Korean Army, the GOP science-based border security system is known to ensure a relatively high detection and identification rate in good weather conditions, but its performance in harsh weather conditions such as rain and fog is somewhat lacking. As an alternative to overcoming this, a radar-based border security system that can detect objects even in bad weather has been proposed. This paper proves the effectiveness of the AI-based scientific border security system based on radar that is being currently tested at the 00th Division through the 2021 Rapid Acquisition Program, and suggests the direction of development for the GOP scientific border security system.

• Journal of Food Hygiene and Safety
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• v.28 no.1
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• pp.50-55
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• 2013

Since 1 June 2012, it is prohibited to sell oilfish as a food material but there are still many illegal cases of selling oilfish as if it is tuna or grilled Patagonian toothfish. So it is absolutely crucial to construct the system to distinguish the real food material from oilfish. There are two sorts of oil fish called Ruvettus pretiosus and Lepidocybirium flavobrunneum involved in Percifomes order and Gempylidae class. 16S DNA gene region in mitochondria was selected to design the specific primers. For design species-specific primer, the theoretical experiment were performed for the sequences of R. pretiosus, L. flavobrunneum, Thunnus thynnus, Thunnus albacores, Makaira mitsukurii and Xiphias gladius, registered at the Gene bank from the National Centre for Biotechnology Information, using BioEdit 7.0.9.0. program. Through the analysis of the result from experiments, it was possible to design the 4 kinds of primers to distinguish R. pretiosus and L. flavobrunneum. As a comparison group, 3 kinds of tuna and 4 kinds of billfishes were selected and experimental verification was performed. As a result, for R. pretiosus and L. flavobrunneum, R.P-16S-006-F/R.P-16S-008-R and L.F-16S-004-F/L.F-16S-006-R primers were selected eventually and PCR condition was established. In addition, 178bp and 238bp of PCR products were confirmed from the established condition and non-specific band was not amplified among similar species. Therefore, the species-specific primers developed in this study would be very useful and used in various ways such as internet shopping mall and illegal distributions with fast and scientific results.