• Steel and Composite Structures
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• v.39 no.2
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• pp.135-148
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• 2021

Hollow structural section (HSS) columns have been increasingly popular due to their structural and architectural merits. However, practical difficulty lies in developing proper connections. The through-diaphragm connections are considered as suitable connection type that is widely adopted in Asian countries. However, the stress concentration occurs at the location connecting through-diaphragm and steel beam. Furthermore, the actual load path from the beam flange is not uniformly transferred to the HSS column as conventionally assumed. In this paper, tensile tests were further conducted on three additional specimens with beam flange plate to evaluate the load versus displacement response. The load-displacement curves, yield and ultimate capacity, ductility ratio were obtained. Furthermore, the strain development at different loading levels was discussed comprehensively. It is shown that the studied connection configuration significantly reduces the stress concentration. Meanwhile, simplified trilinear load-displacement analytical model for specimen under tensile load was presented. Good agreement was found between the theoretical and experimental results.

• BMB Reports
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• v.54 no.11
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• pp.551-556
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• 2021

In this study, we investigated how Staufen1 influences the HIV-1 production. The overexpression of Staufen1 increased virus production without any negative affect on the viral infectivity. This increase was not caused by transcriptional activation; but by influencing post-transcriptional steps. Using multiple Gag protein derivatives, we confirmed that the zinc-finger domains of the HIV-1 nucleocapsid (NC) are important for its interaction with Staufen1. We also found that Staufen1 colocalized in stress granules with the mature form of the HIV-1 NC protein.

• Nuclear Engineering and Technology
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• v.55 no.1
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• pp.12-24
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• 2023

With the improvement of laser technology, the strategic needs of efficient and precise decontamination of various components in nuclear application units can be fulfilled by laser decontamination. The surface contaminants of nuclear facilities mainly exist both as loose contaminated layer and fixed oxide layer. The types of radionuclides and contamination layer thickness are closely related to the operation status of nuclear facilities, which have an important influence on the laser decontamination process. This study reviewed the mechanism of laser surface treatment and the influence of laser process parameters on the decontamination thickness, decontamination factor, decontamination efficiency and the distribution of aerosol particle. Although multiple studies have been performed on the mechanism of laser processing and laser decontamination process, there are few studies on the microscopic process mechanism of laser decontamination and the influence of laser decontamination on surface properties. In particular, the interaction between laser and radioactive contaminants needs more research in the future.

• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.1113-1114
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• 2004

• The Korea Genome Conference
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• 2003.09a
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• pp.43.1-43.1
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• 2003

• Journal of The Korean Association For Science Education
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• v.17 no.4
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• pp.501-510
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• 1997

Science teachers' beliefs about science and school science and their perceptions of the science laboratory learning environment were investigated with an assumption that science laboratory teaching would be affected by science teachers' beliefs. Likert-scale questionnaires of BASSSQ and SLEI were used in this study. The major findings were as follows: 1. Science teachers showed inconsistent beliefs about science and school science. Their responses reflected a patch-like view of postmodern epistemology and objectivism They also showed somewhat different views about science and school science. It was found that science teachers had strong objectivist views about science in some parts. but they had moderate constructivist views about school science in other parts; 2. The mean scores of student cohesiveness, integration. and rule clarity on the actual version in SLEl were relatively high, but those of open-endedness and physical environment were very low; 3. There was no association between teachers' beliefs about science and their perceptions of the science laboratory learning environment. But some associations were found between teachers' beliefs about school science and their perception on student cohesiveness, integration, and rule clarity of the actual science laboratory learning environment. Teachers' beliefs about school science had some statistically significant correlations with their perceptions on all scales of the preferred version of SLEI. We could not show a causal relationship between teachers' beliefs and their science laboratory learning environment through these results. But it can be suggested that teachers' beliefs about school science do have a role in constructing a desirable science laboratory learning environment, as we found that there were statistically significant correlations between them.

• Journal of Veterinary Science
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• v.22 no.1
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• pp.8.1-8.11
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• 2021

Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

• Nuclear Engineering and Technology
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• v.52 no.7
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• pp.1386-1395
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• 2020

High fidelity nuclear reactor fuel assembly simulation using CFD method is an effective way for the structure design and optimization. The validated models and user practice guidelines play critical roles in achieving reliable results in CFD simulations. In this paper, the international benchmark MATiS-H is studied carefully and the best user practice guideline is achieved for the rod bundles simulation. Then a 5 × 5 rod bundles model in the advanced pressurized water reactor (PWR) is established and the detailed three-dimensional thermal-hydraulic characteristics are investigated. The influence of spacer grids and mixing vanes on the flow and hear transfer in rod bundles is revealed. As the coolant flows through the spacer grids and mixing vanes in the rod bundles, the drastic lateral flow would be induced and the pressure drop increases significantly. In addition, the heat transfer is enhanced remarkably due to the strong mixing effects. The calculation results could provide meaningful guidelines for the design of advanced PWR fuel assembly.

• Nuclear Engineering and Technology
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• v.53 no.10
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• pp.3217-3228
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• 2021

Flow blockage of the fuel assembly in the lead-based fast reactor (LFR) may produce critical local spots, which will result in cladding failure and threaten reactor safety. In this study, the flow blockage characteristics were analyzed with the sub-channel analysis method, and the circumferentially-varied method was employed for considering the non-uniform distribution of circumferential temperature. The developed sub-channel analysis code SACOS-PB was validated by a heat transfer experiment in a blocked 19-rod bundle cooled by lead-bismuth eutectic. The deviations between the predicted coolant temperature and experimental values are within ±5%, including small and large flow blockage scenarios. And the temperature distributions of the fuel rod could be better simulated by the circumferentially-varied method for the small blockage scenario. Based on the validated code, the analysis of blockage characteristics was conducted. It could be seen from the temperature and flow distributions that a large blockage accident is more destructive compared with a small one. The sensitivity analysis shows that the closer the blockage location is to the exit, the more dangerous the accident is. Similarly, a larger blockage length will lead to a more serious case. And a higher exit temperature will be generated resulting from a higher peak coolant temperature of the blocked region. This work could provide a reference for the future design and development of the LFR.

• Genes and Genomics
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• v.40 no.11
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• pp.1169-1180
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• 2018

Cave shrimps from the genera Typhlatya, Stygiocaris and Typhlopatsa (TST complex) comprises twenty cave-adapted taxa, which mainly occur in the anchialine environment. Anchialine habitats may undergo drastic environmental fluctuations, including spatial and temporal changes in salinity, temperature, and dissolved oxygen content. Previous studies of crustaceans from anchialine caves suggest that they have possessed morphological, behavioral, and physiological adaptations to cope with the extreme conditions, similar to other cave-dwelling crustaceans. However, the genetic basis has not been thoroughly explored in crustaceans from anchialine habitats, which can experience hypoxic regimes. To test whether the TST shrimp-complex hypoxia adaptations matched adaptive evolution of mitochondrial OXPHOS genes. The 13 OXPHOS genes from mitochondrial genomes of 98 shrimps and 1 outgroup were examined. For each of these genes was investigated and compared to orthologous sequences using both gene (i.e. branch-site and Datamonkey) and protein (i.e. TreeSAAP) level approaches. Positive selection was detected in 11 of the 13 candidate genes, and the radical amino acid changes sites scattered throughout the entire TST complex phylogeny. Additionally, a series of parallel/convergent amino acid substitutions were identified in mitochondrial OXPHOS genes of TST complex shrimps, which reflect functional convergence or similar genetic mechanisms of cave adaptation. The extensive occurrence of positive selection is suggestive of their essential role in adaptation to hypoxic anchialine environment, and further implying that TST complex shrimps might have acquired a finely capacity for energy metabolism. These results provided some new insights into the genetic basis of anchialine hypoxia adaptation.