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http://dx.doi.org/10.5483/BMBRep.2021.54.11.072

Investigation of the effect of Staufen1 overexpression on the HIV-1 virus production  

Park, Seong-won (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Yu, Kyung-Lee (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Bae, Jun-Hyun (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Kim, Ga-Na (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Kim, Hae-In (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Publication Information
BMB Reports / v.54, no.11, 2021 , pp. 551-556 More about this Journal
Abstract
In this study, we investigated how Staufen1 influences the HIV-1 production. The overexpression of Staufen1 increased virus production without any negative affect on the viral infectivity. This increase was not caused by transcriptional activation; but by influencing post-transcriptional steps. Using multiple Gag protein derivatives, we confirmed that the zinc-finger domains of the HIV-1 nucleocapsid (NC) are important for its interaction with Staufen1. We also found that Staufen1 colocalized in stress granules with the mature form of the HIV-1 NC protein.
Keywords
HIV-1; RNA-binding proteins; Staufen1; Virus-host interaction; Virus replication;
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