• Title/Summary/Keyword: scanning microscopy

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Light and Scanning Electron Microscopic Study on the Structure of the Gastrointestinal Mucosa of the Mole, Talpa micrura coreana (두더지 (Talpa micrura coreana) 위장관 점막의 구조에 대한 광학 및 주사전자현미경적 연구)

  • Kwun, H.S.;Chung, J.W.;Chun, M.H.;Kim, J.;Cha, J.H.;Maeng, S.H.
    • Applied Microscopy
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    • v.15 no.2
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    • pp.111-129
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    • 1985
  • The morphology and histology of the gastrointestinal mucosa of the mole, Talpa micrura coreana (Thomas), were studied using light and scanning electron microscopes. Tissue specimens were taken from body and pyloric portions of the stomach, and from the initial, proximal, middle, distal and terminal portions of the intestine. For light microscopy, tissue blocks were fixed in 10% buffered neutral formalin, embedded in paraffin wax, and sectioned at a thickness of $5{\mu}m$. These sections were stained with hematoxylin-eosin. For scanning electron microscopy, tissue blocks were fixed in 1% glutaraldehyde-1.5% paraformaldehyde, and postfixed in 1% osmium tetroxide, dehydrated in graded alcohol, transferred to isoamylacetate and dried by the critical point drier(Polaron E 3000). Subsequently, specimens were coated with gold and observed with a JSM-35C scanning electron microscope. The results were as follows: The mucous membrane of the body portion of the stomach had numerous irregular folds and the pyloric mucosa formed the strawberry-shaped folds, and general histological structures of each portion were similar to those of man. The intestine could not be differentiated macroscopically and microscopically into small and large intestines. There was no cecum, appendix, taenia coli, haustra coli or appendices epiploicae. In the initial portion (4 mm long), conical or tongue-shaped villi with the height of $143.3{\pm}10.7{\mu}m$ were present, and large mucous glands were seen in the submucosa. In the proximal, middle and distal portions, wavy folds composed of the epithelium and lamina propria were densely and transversely arranged, and their heights were $440.4{\pm}45.5{\mu}m,\;454.4{\pm}19.9{\mu}m\;and\;205.2{\pm}33.5{\mu}m$, respectively. The mucosa of the terminal portion (3 cm long) formed several longitudinal folds, and the intestinal glands were directly opened on the smooth surface of the folds. Aggregated lymphoid follicles were observed in the major portions of the intestine except the initial and terminal portions. There was no circular or semilunar fold throughout the intestine.

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Self-focusing and Structure Measurement of $a-As_2S_3$ Using Near-field Scanning Optical Microscopy (근접장 광학 현미경을 이용한 $a-As_2S_3$ 박막에서의 자체집광 및 구조변화 측정)

  • 전태종;정희성;조규만
    • Proceedings of the Optical Society of Korea Conference
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    • 2002.07a
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    • pp.122-123
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    • 2002
  • 능동적으로 안정화시킨 반도체 레이저의 빛살되먹임을 이용한 근접장 광학현미경(Near-field Scanning Optical Microscope)을 이용하여 칼코게나이드계 유리인 a-As$_2$S$_3$에서 광조사에 따른 자체집광 현상과 구조변화를 관찰하였다. 여러 비선형 광학현상 중 광 커르 효과(Optical Kerr effect)에 기인하는 자체집광(Self-focusing)은 광범위하게 연구되어 왔다. (중략)

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Development of high-speed near-field scanning optical microscopy by using a high-frequency quartz-crystal resonator (고주파 수정진동자를 이용한 고속 근접장 광학 현미경 제작)

  • 서용호;제원호
    • Proceedings of the Optical Society of Korea Conference
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    • 2001.02a
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    • pp.120-121
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    • 2001
  • 고분해능을 장점으로 하는 근접장 광학 현미경(NSOM)은 최근 높은 관심을 모으고 있으나, 그 촬영 속도가 너무 느린 이유로 실제적인 활용이 미비한 상황이다. scanning 속도를 높이고자 하는 노력들이 많이 진행되고 있으나[1-2] 아직도 실시간 촬영과는 거리가 멀다. 기존의 근접장 광학 현미경(NSOM)은 tuning fork를 사용하여 탐침을 진동시키고 그 진동의 진폭 또는 위상의 변화를 측정함으로써 탐침과 시료의 거리를 측정하는 방식을 사용한다. (중략)

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A Note on Biogenic Effects of Coralloid Speleothems in Round Mountain Lava Cave, Oregon, U.S.A.

  • Kashima, Naruhiko;Ogawa, Takanori
    • Journal of the speleological society of Korea
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    • v.9
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    • pp.3-7
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    • 1999
  • Corralloid speleothems from Round Mountain lave cave are are studied by scanning microscope and electron microprobe analyses. Scanning microscopy observation indicates that the diatom Melosira seems to contribute significantly to siliceous coralloid speleothems Electron microprobe confirms the presence of diatom and fragmental minerals(plagioclase and orthopyroxene) in coralloid speleothems. Chemical analysis of 3 diatom cells gives SiO2(74.8%), Al2O3(0.12%), FeO(0.11%), CaO(0.47%) and MgO(0.81%).

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Chitosan-Iron casein succinylate nanoparticles as oral delivery systems: increasing the stability and enhancing the absorption of iron nanoparticles.

  • Cho, Jung-Hye;Oungbho Kwunchit;Park, Jeong-Sook;Kim, Chong-Kook
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.249.1-249.1
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    • 2003
  • The objective of the study was to develop an oral delivery system to increase the stability and efficacy of iron casein succinylate. Aqueous nanoparticles were prepared using complex coacervation of the oppositely charged chitosan and iron casein succinylate with polyethyleneglycol (PEG). The physicochemical properties of nanoparticles were investigated using dynamic light scattering, zeta potential and scanning electron microscopy. Chitosan-iron casein succinylate interactions were investigated in solid state by differential scanning calorimetry (DSC) and FT-IR spectrometry. (omitted)

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Conductivity of copper(II)-phthalocyanine thin films due to a grain growth (결정 성장 조건에 따른 copper(II)-phthalocyanine 박막의 전기전도도 특성)

  • Park, Mie-Hwa;Yoo, Hyun-Jun;Lee, Kie-Jin
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.05a
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    • pp.132-136
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    • 2004
  • 열 증착 방법을 이용하여 copper(II)-phthalocyanine(CuPc) 박막을 glass 기판 위에 제작하였다. 열처리 조건은 $150^{\circ}C$에서 후열(annealing) 처리 하는 방식과 예열하는 두 가지 방식으로 달리하였다. 제작된 박막의 전기전도도를 평가하기 위해 마이크로웨이브 근접장 효과를 이용한 근접장 현미경(near-field scanning microwave microscope)을 이용하여 비파괴적인 방식으로 CuPc 박막의 반사계수(reflection coefficient)를 측정하였다. CuPc 박막의 전기전도도 특성을 UV 흡수도를 통한 에너지 밴드갭의 shift 현상과 관련지어 설명하고 또한 x-ray diffraction(XRD) data를 통해 박막의 결정 특성과 비교하였다. 박막 표면 특성은 SEM(scanning microscope microscopy)을 통해 관측하였다. 열처리 조건에 따른 CuPc 박막의 전기전도도 특성은 후열 처리한 박막의 경우 예열 처리한 박막보다 전기 전도 특성이 향상되었음을 관측할 수 있었다.

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Electronic Structures of Graphene on Ru(0001) : Scanning Tunneling Spectroscopy Study

  • Jang, Won-Jun;Jeon, Jeung-Hum;Yoon, Jong-Keon;Kahng, Se-Jong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.307-307
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    • 2011
  • Graphene is the hottest topic in condensed-matter physics due to its unusual electronic structures such as Dirac cones and massless linear dispersions. Graphene can be epitaxially grown on various metal surfaces with chemical vapor deposition processes. Such epitaxial graphene shows modified electronic structures caused by substrates. Here, local geometric and electronic structures of graphene grown on Ru(0001) will be presented. Scanning tunneling microscopy (STM) and spectroscopy (STS) was used to reveal energy dependent atomic level topography and position-dependent differential conductance spectra. Both topography and spectra show variations from three different locations in rippled structures caused by lattice mismatch between graphene and substrate. Based on the observed results, structural models for graphene on Ru(0001) system were considered.

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Visualization of Epidermis and Dermal Cells in ex vivo Human Skin Using the Confocal and Two-photon Microscopy

  • Choi, Sang-Hoon;Kim, Wi-Han;Lee, Yong-Joong;Lee, Ho;Lee, Weon-Ju;Yang, Jung-Dug;Shim, Jong-Won;Kim, Jin-Woong
    • Journal of the Optical Society of Korea
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    • v.15 no.1
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    • pp.61-67
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    • 2011
  • The confocal laser scanning microscopy and two-photon microscopy was implemented based on a single laser source and an objective lens. We imaged and compared the morphology of identical sites of ex vivo human skin using both microscopes. The back-scattering emission from the sample provided the contrast for the confocal microscopy. The intrinsic autofluorescence and the second harmonic generation were used as the luminescence source for the two-photon microscopy. The wavelength of the Ti:Sapphire laser was tuned at 710 nm, which corresponds to the excitation peak of NADH and FAD in skin tissue. The various cell layers in the epidermis and the papillary dermis were clearly distinguished by both imaging modalities. The two-photon microscopy more clearly visualized the intercellular region and the nucleus of the cell compared to the confocal microscopy. The fibrous structures in the dermis were more clearly resolved by the confocal microscopy. Numerous cells in papillary dermal layer, as deep as $100\;{\mu}m$, were observed in both CLSM and two-photon microscopy. While most previous studies focused on fibrous structure imaging (collagen and elastin fiber) in the dermis, we demonstrated that the combined imaging with the CLSM and two-photon microscopy can be applied for the non-invasive study of the population, distribution and metabolism of papillary dermal cells in skin.

Preparation Method of Plan-View Transmission Electron Microscopy Specimen of the Cu Thin-Film Layer on Silicon Substrate Using the Focused Ion Beam with Gas-Assisted Etch

  • Kim, Ji-Soo;Nam, Sang-Yeol;Choi, Young-Hwan;Park, Ju-Cheol
    • Applied Microscopy
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    • v.45 no.4
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    • pp.195-198
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    • 2015
  • Gas-assisted etching (GAE) with focused ion beam (FIB) was applied to prepare plan-view specimens of Cu thin-layer on a silicon substrate for transmission electron microscopy (TEM). GAE using $XeF_2$ gas selectively etched the silicon substrate without volume loss of the Cu thin-layer. The plan-view specimen of the Cu thin film prepared by FIB milling with GAE was observed by scanning electron microscopy and $C_S$-corrected high-resolution TEM to estimate the size and microstructure of the TEM specimen. The GAE with FIB technique overcame various artifacts of conventional FIB milling technique such as bending, shrinking and non-uniform thickness of the TEM specimens. The Cu thin film was uniform in thickness and relatively larger in size despite of the thickness of <200 nm.