• Title/Summary/Keyword: scanning microscopy

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The bactericidal effect of an atmospheric-pressure plasma jet on Porphyromonas gingivalis biofilms on sandblasted and acid-etched titanium discs

  • Lee, Ji-Yoon;Kim, Kyoung-Hwa;Park, Shin-Young;Yoon, Sung-Young;Kim, Gon-Ho;Lee, Yong-Moo;Rhyu, In-Chul;Seol, Yang-Jo
    • Journal of Periodontal and Implant Science
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    • v.49 no.5
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    • pp.319-329
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    • 2019
  • Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.

Synthesis and characterization of starch$^Na+$-montmorillonite clay nanocomposites

  • Na, Seong-Ki;Park, Jong-Shin
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10a
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    • pp.67-68
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    • 2003
  • Native corn starch and montmorillonite caly nanocomposites were prepared using the glycerol as the plasticizer. These were characterized by mechanical analysis, X-ray diffraction, infrared spectroscopy, differential scanning calorimetry, and scanning electron microscopy. The tensile strength increased with the clay content to a maximum point and then decreased due to gapping between the two phases. Dispersion of the layered silicate within the starch was verified using X-ray diffraction pattern. Examination of these materials by scanning electron showed that intercalates have good wetting to the starch surface.

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Construction of Ultra High Vacuum Scanning Tunneling Microscope (초고진공 Scanning Tunneling Microscope의 제작)

  • Son, Eun-Sook;Hong, Yeong-Kyu;Park, Chan
    • Journal of the Korean Vacuum Society
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    • v.3 no.4
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    • pp.377-381
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    • 1994
  • 초고진공(UHV) Scanning Tummeling Microscopy(STM)을 제작하였다. 8인치 프란지에 부착한 STM은 초고진공에서 시료의 통전가열이 가능하며 다른 표면 측정방법의 적용과시료처리가 용이하다. 외부로부터 초고진공을 깨지 않고 시료와 tip의 도입이 가능하며 tip을 가열할 수 있다. 완성된 장치로 Si(111)-7$\times$7 구조의 STM상을 얻었다.

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A Study on the Coupling of a Flanged Parallel-Plate Waveguide to a Nearby Conducting Strip from the Viewpoint of Near-Field Scanning Microscopy (근접주사현미경의 관점에서 플랜지된 평행평판 도파관과 근접도체스트립과의 결합에 관한 연구)

  • Lee, Jong-Ig;Ko, Ji-Hwan;Cho, Young-Ki
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.11
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    • pp.2260-2266
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    • 2009
  • In this paper, the problem of electromagnetic coupling between a slit fed by a flanged parallel-plate waveguide (FPPW) and a nearby conducting strip parallel to the slit is studied as a simplified problem for a near-field scanning microscopy (NSM). The characteristics of the FPPW are investigated from the results for the variations of the equivalent slit admittance, the reactive powers near the slit inside and outside the FPPW, the magnitude and phase of the voltage reflection coefficient of the TEM wave. The performance of the proposed apparatus as an NSM is tested by examining the effects of various geometrical parameters such as guide height, slit width, strip width, distance between slit and strip, and the ratio of slit width to guide height on the magnitude and phase of the voltage reflection coefficient of the TEM wave. From the results for the voltage reflection coefficient against the strip offset from the slit, it is found that a slit in the FPPW with smaller guide height gives higher scanning resolution and the phase variation is more sensitive than the magnitude variation.