• Applied Microscopy
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• 제51권
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• pp.16.1-16.7
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• 2021

Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.

• 한국측량학회지
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• 제33권2호
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• pp.111-122
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• 2015

본 연구에서는 모바일 레이저 스캐닝 데이터로부터 철도 선로탐지 및 선로모델 추출을 위한 방법을 제시하였다. 제안된 방법은 크게 세 단계로 구성된다. 첫째, 레이저 포인트로부터 잠재적인 철도 선로지역을 탐지하고, 초기 철도 선로궤적 방향을 추정한다. 둘째, 철도 선로에 관한 선 지식을 이용하여 첫번째 스트립에서 초기 선로위치를 결정한다. 여기서, 스트립은 국부 탐색공간을 나타내며 철도 선로궤적에 수직인 방향으로 정의된다. 마지막으로, 초기 선로위치에서 GMM-EM기반 분류방법을 통해 선로 포인트들을 탐지한 후 초기 선로 모델을 생성하고 스트립을 데이터 처리 기본단위로 하여 tracking by detection관점에서 연속적으로 선로모델을 생성하였다. 제안된 방법의 주요 특징은 다음과 같다. 첫째, 이전 스트립에서 생성된 선로 모델을 가이드 라인으로 다음 스트립에 전파되어 국부 탐색영역을 예측하여 선로 포인트를 탐지하는 하는데 있어서 처리 복잡성을 줄일 수 있었다. 둘째, 선로 포인트 탐지와 선로 모델링을 동시에 진행 함으로써 데이터 처리 시간을 최소화 할 수 있었다. 개발된 알고리즘은 C++ 프로그램 언어로 구현되었고 도시지역에서 MMS 측량을 통해 취득된 LiDAR 데이터(경부선 일부 구간)를 이용하여 성능 테스트를 진행하였다.

• 지질공학
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• 제9권1호
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• pp.45-57
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• 1999

암반에서의 지하수유동은 그 지역에 발달된 단열(fracture)에 의해 지배된다. 그러므로 결정질암에서의 지하수유동에 있어서 연결성이 좋은 단열대의 존재는 매우 중요하다. 본 연구는 유성북부 (위도 $36^{\circ}24'18"~36^{\circ}25'08",{\;}경도{\;}127^{\circ}21'00"~127^{\circ}23'23"$)에 분포하고 있는 중생대 화강암지역에서 지하수의주 유동로가 되는 단열의 분포특성을 파악하는데 목적이 있다. 비유일성해석 특성을 갖는 지구물리탐사의 단점을 보완하기 위해서 기존에 조사된 logging 자료, 수위자료, BHTV자료, 수리시험자료해석을 바탕으로 전기탐사(DC)와 전자탐사(EM)를 실시하였다. 전기탐사로는 슐럼버저와 쌍극자배열인 전기비저항탐사를 실시하였으며, 잔자탐사로 극저주파 전자탐사(VLF-EM)를 병행하였다. 기 조사자료와 지구물리탐사의 결과를 종합하여 분석한 결과, 연구지역의 투수성 단열의 분포 특성은 염기성암맥과 암맥 주변에 발달된 연결성이 좋은 단열체계 계곡방향인 NNW방향을 가로질러 지하수의 주 유동로를 막는 수리적 방벽 역할이다. 그러므로 본 연구지역에서의 등수위선분포는 지형의 고저와 무관하게 고지대의 수리수두가 저지대의 수리수두보다 낮은 현상이 일어난다.

• Journal of electromagnetic engineering and science
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• 제16권3호
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• pp.150-158
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• 2016

This paper reports on a study of LiNiZn-ferrite composite as a radiation absorbent material (RAM). The electromagnetic (EM) wave absorbers are composed of an EM wave absorbing material and a polymeric binder. The surface morphology, chemical composition, weight percent of the ferrite composite of the toroid sample, magnetic properties, and return loss are investigated using field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and network analyzer. For preparing the absorbing sheet, chlorinated polyethylene (CPE) is used as a polymeric binder. The EM wave absorption properties of the prepared samples were studied at 4 - 8 GHz. We can confirm the effects of the thickness of the samples for absorption properties. An absorption bandwidth of more than a 10-dB return loss shifts toward a lower frequency range along with an increase in the thickness of the absorber.

• 한국축산식품학회지
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• 제39권6호
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• pp.1008-1014
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• 2019

This study investigated the effects of pressure-mediated protein changes on the ice nucleation temperature of pork loins. To variate chemical state of meat proteins, pork loin was pressurized at varying pressure levels (100-500 MPa) for 3 min, and moisture content, expressible moisture (EM) and differential scanning calorimetry (DSC) were analyzed. Although, all treatments showed similar moisture content, EM and degree of protein unfolding of pork loin showed different features as of 300 MPa. At moderate pressure treatments (100-200 MPa), all protein fractions were detected in DSC experiments, and pork loin had lower EM than control (p<0.05). Meanwhile, myosin and actin of pork loin treated at greater than 300 MPa were completely unfolded, and the treatments showed high EM compared to control (p<0.05). Unfolding of meat proteins was a factor suppressing ice nucleation, and the ice nucleation temperature tended to decrease with increasing applied pressure level. The ice nucleation characteristics of pressurized pork loin exhibited a potential application in freezing storage of pressurized meat with less tissue damage comparing to freeze fresh meat, and further exploration regarding the quality change after freezing of fresh and pressurized meat was warranted.

• Journal of Pharmaceutical Investigation
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• 제37권1호
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• pp.15-22
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• 2007

An oil-in-water solvent evaporation method was used to prepare the cyclosporin A (CyA)-loaded nanoparticles varying in poly (D,L-lactide-co-glycolide) (PLGA) polymer (RG 502H, RG 503H) and the amount of additive ethyl myristate (EM) or chitosan (CS). The particles were characterized for drug loading and entrapment efficiency by HPLC, surface morphology by scanning electron microscopy, particle size by dynamic light scattering and surface charge by Zetapotential. The results showed drug loadings ranging from 10.9% to 15.8% with high encapsulation efficiency (82.0-97.8%). SEM and DLS studies showed discrete and spherical particles with smooth surfaces and mean size ranging 257.6-721.7 nm. The additive EM or CS did not change the mean sizes of the nanoparticles, whereas by the coating effect of CS, the Zetapotential values of the CS-added nanoparticles were moved to the more positive direction as the amount of CS was increased. From the pharmacokinetic analysis, the nanoparticles formulations showed the higher bioavailability and MRT than $Neoral^{\circledR}$ While little adding effect of EM or CS was detected in pharmacokinetic profile when RG 503H was used as polymer carrier, more noticeable different pharmacokinetic behaviors could be observed in case of RC 502H. EM incorporation was found to elevate the $K_{el}$, whereas CS coating resulted in the decrease of F and $K_{el}$, which seems to be due to the function of CS as a barrier and a mucoadhesive coating.

• 마이크로전자및패키징학회지
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• 제22권2호
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• pp.47-53
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• 2015

인쇄회로기판 솔더 상부 및 하부 접합부의 서로 다른 표면처리 조건에 따른 Sn-3.0Ag-0.5Cu (SAC305) 접합부의 열처리 및 전류 인가에 따른 금속간 화합물 성장거동을 비교하기 위하여 in-situ 미세구조분석 및 electromigration (EM) 수명평가를 실시하였다. 솔더 접합 직후, 상부 접합부의 electroless nickel immersion gold (ENIG) 표면처리에서는 $(Cu,Ni)_6Sn_5$, 하부 접합부의 organic solderability preservative (OSP) 표면처리에서는$ Cu_6Sn_5$, $Cu_3Sn$ 금속간 화합물이 접합 계면에서 생성되었다. EM 수명평가 결과 온도 $130^{\circ}C$, 전류밀도 $5.0{\times}10^3A/cm^2$ 하에서 평균파괴시간이 약 78.7 hrs으로 도출되었고, 하부 OSP 표면처리에서 전자가 솔더로 빠져나가는 부분에서 Cu의 소모에 의한 단락이 주 손상기구로 확인되었다. In-situ 주사전자현미경을 통해 계면 미세구조 분석 결과 상부 접합부 ENIG 표면처리에서 전자의 방향에 따른 미세구조의 큰 차이가 없고 뚜렷한 손상이 관찰되지 않았으나, 하부 접합부 OSP 표면처리의 경우 전자가 솔더로 유입되는 부분에서 빠른 Cu 소모로 인한 보이드 성장이 관찰되었다. 따라서, SAC305무연솔더 접합부에서 ENIG 표면처리가 OSP 표면처리보다 보다 우수한 EM확산방지막 역할을 하여 금속간 화합물 성장을 억제하고 보다 우수한 EM 신뢰성을 보이는 것으로 판단된다.

• 한국식품과학회지
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• 제32권2호
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• pp.258-264
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• 2000

8품종 변이체 쌀로부터 전분을 분리하여 몇몇 이화학적 특성을 비교하였다. 일반품종 및 저아밀로오스 변이체 쌀 전분입자의 형태는 다변형인데 비해서 shr 및 분질미 전분입자의 형태는 구형을 나타내고 있었다. 남풍 CB243의 호화개시온도 및 호화종료온도가 특히 높았으며, 분질미 및 남풍 EM90의 호화엔탈피가 다른 품종들에 비해서 높았다. 15% $H_2SO_4$에 대한 가수분해도는 shr.이 가장 높았으며, 남풍 CB243이 가장 낮았다. X선 회절도는 8품종 모두 전형적인 A형이었다. 저아밀로오스 유전자가 도입된 품종일수록 glucoamylase에 의한 효소 가수분해도는 증가하고 있었다.

• Journal of Animal Science and Technology
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• 제62권4호
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• pp.577-586
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• 2020

This study was to investigate the effect of salt alone or in combination with phosphate on physicochemical and textural properties, and chemical interactions of low-fat model sausages. pH, color, expressible moisture (EM), cooking loss (CL), proximate analysis, textural profile analysis and low-vacuum scanning electron microscopy were performed. As salt content increased, color tended to decrease, as did EM and CL parameters, indicating that the ability to retain moisture was improved with increased salt levels (p < 0.05). In addition, textural hardness, gumminess and chewiness all increased with increasing salt (p < 0.05). Sausages with 0.3% salt showed the lowest cohesiveness compared to those with salt levels higher than 0.3% (p < 0.05). Addition of sodium tripolyphosphate (STPP) increased pH of sausages. Increasing salt and STPP did not affect lightness (p > 0.05), but did increase redness and yellowness (p < 0.05). The moisture content was higher when the salt and STPP contents were increased (p < 0.05), but no differences in the fat and protein contents (%) were observed (p > 0.05). EM and CL tended to decrease with increasing salt and STPP. In textural properties, the combination of 1.8% salt and 0.3% STPP was the best among other treatment (p < 0.05). Surface microstructure showed a flat and dense structure with increasing salt and STPP. Since the addition of salt and phosphate improved the functionality, textural and physicochemical properties of meat products in this study, meat products will need to be developed in line with consumer's preference.

• 대한기관식도과학회지
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• 제16권1호
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• pp.33-38
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• 2010

Titania nanomaterials are widely used as cosmetics and dyes, however the impacts on human health are uncertain, We investigated the biodistribution of inhaled titania nanoparticles in rats, Methods Eight weeks-old SD rats were intubated and inhaled with 3 mg titania nanoparticles, twice a week, for 2 weeks, After inhalation, the rats were sacrificed and tissues or heart, lung. intestine, brain, and liver were obtained, We investigated the tissues with optical microscope (OM), transmission electron microscope (EM), scanning EM, And to analyze titania concentration of each tissue, we lysed the tissues with radioimmunoprecipitation assay (RlPA) lysis buffer or acid. Results Granulation tissues in lung were confirmed on the optical microscope, however the other organs had no abnormalities in OM images, In EM images, the rats which inhaled titania nanoparticles showed calcium deposition at heart, brain, and intestine, Titania concentration in lung was increased on the inhaled rat sacrificed I month after last exposure. Conclusion Inhaled titania nanoparticles is thought to be deposited and make inflammatory reaction in lung, and the deposition was not efficiently cleared over a month. However inhaled titania nanoparticles may rarely pass through the alveolus-blood barrier and distribute to other organs of the bod.