• Title/Summary/Keyword: scale-up technology

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Comparative Production of Green Fluorescent Protein Under Co-expression of Bacterial Hemoglobin in Escherichia coli W3110 Using Different Culture Scales

  • Bassapa Johnvesly;Kang, Dong-Gyun;Park, Suk-Soon;Kim, Ji-Hyun;Cha, Hyung-Joon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.4
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    • pp.274-277
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    • 2004
  • Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.

Elliptic Feature of Coherent Fine Scale Eddies in Turbulent Channel Flows

  • Kang Shin-Jeong;Tanahashi Mamoru;Miyauchi Toshio
    • Journal of Mechanical Science and Technology
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    • v.20 no.2
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    • pp.262-270
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    • 2006
  • Direct numerical simulations (DNS) of turbulent channel flows up to $Re_{\tau}=1270$ are performed to investigate an elliptic feature and strain rate field on cross sections of coherent fine scale eddies (CFSEs) in wall turbulence. From DNS results, the CFSEs are educed and the strain rate field around the eddy is analyzed statistically. The principal strain rates (i.e. eigenvalues of the strain rate tensor) at the CFSE centers are scaled by the Kolmogorov length $\eta$ and velocity $U_k$. The most expected maximum (stretching) and minimum (compressing) eigenvalues at the CFSE centers are independent of the Reynolds number in each $y^+$ region (i. e. near-wall, logarithmic and wake regions). The elliptic feature of the CFSE is observed in the distribution of phase-averaged azimuthal velocity on a plane perpendicular to the rotating axis of the CFSE $(\omega_c)$. Except near the wall, phase-averaged maximum $(\gamma^{\ast}/\gamma_c^{\ast})$ and minimum $(\alpha^{\ast}/\alpha_c^{\ast})$ an eigenvalues show maxima on the major axis around the CFSE and minima on the minor axis near the CFSE center. This results in high energy dissipation rate around the CFSE.

Large-Scale Experiments on the Wave-Induced Liquefaction of Sandbed (파랑작용에 의한 모래지반의 액상화에 관한 대형 수리모형실험)

  • Kang, Yoon-Koo;Suzuki, Kojiro
    • Journal of Ocean Engineering and Technology
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    • v.21 no.3 s.76
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    • pp.26-32
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    • 2007
  • A series of large-scale experiments were carried out in order to examine wave-induced liquefaction in a loosely packed sandbed, its afterward high densification and liquefaction by oscillatory pore pressure. The experiments were conducted in a Large Hydro-Geo Flume that can nearly solve the problems of scale effects of the sandbed, and the 50% sieve diameter of sand was 0.2 mm. The generation of residual pore pressure and its afterward high densification which had observed by Takahashi et al. (1999) in a wave flume experiment using fine sand with the size of 0.08 mm. As a result, the relative density of the sandbed after high densification was increased up to 79% and liquefaction by oscillatory pore pressure was not observed.

A Study On Preprocessing of Fingerprint Image Using Multi-Scale Roof Edges (다척도 지붕에지 검출방법을 이용한 지문영상의 전처리에 대한 연구)

  • Kim Soo Gyeam
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.2
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    • pp.217-224
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    • 2005
  • A new roof edge detection method based on multi level scales of wavelet function is proposed in this paper roof edge and its direction are obtained in this new methods at one time. Besides. scale characteristics of detecting roof edge is analyzed. And a few new methods on fingerprint image pre-processing are described. A method segmenting foreground/background of fingerprint images is proposed, in which Prior estimation of direction field is not required any more. A segmentation method based on multi-scale roof edges is implemented. and the valid scale range of the method is defined. too. And the method is used to segment ridges and valleys in fingerprint images simultaneously The exact direction fields made up of the direction of each point in ridges can be obtained when detecting ridges exactly based on the roof edge detector, in comparison with the traditional coarse estimation of direction fields. Obviously. it will establish a solid foundation for the sequent fingerprint identification.

Providing scalable single-operating-system NUMA abstraction of physically discrete resources

  • Baik Song An;Myung Hoon Cha;Sang-Min Lee;Won Hyuk Yang;Hong Yeon Kim
    • ETRI Journal
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    • v.46 no.3
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    • pp.501-512
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    • 2024
  • With an explosive increase of data produced annually, researchers have been attempting to develop solutions for systems that can effectively handle large amounts of data. Single-operating-system (OS) non-uniform memory access (NUMA) abstraction technology is an important technology that ensures the compatibility of single-node programming interfaces across multiple nodes owing to its higher cost efficiency compared with scale-up systems. However, existing technologies have not been successful in optimizing user performance. In this paper, we introduce a single-OS NUMA abstraction technology that ensures full compatibility with the existing OS while improving the performance at both hypervisor and guest levels. Benchmark results show that the proposed technique can improve performance by up to 4.74× on average in terms of execution time compared with the existing state-of-the-art opensource technology.

RECOVERY OF METALS FROM EAF DUST WITH RAPID SYSTEM

  • Shin, Hyoung-ky;Moon, Seok-min;Jhung, Sung-sil
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.381-386
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    • 2001
  • The dust generated from the electric arc furnace steel making process is classified as hazardous material by Korean Environmental Protection Acts, mainly because of the existence of water teachable Pb, Zn and Cd. Thus the treatment of EAF dust is being carried out to fulfill both the environmental aspect and recovery of valuable metals. To establish the proper process for recovering the valuable metals (Fe, Zn, Pb and Cd) and producing the non-toxic slag from EAF dust, using RAPID-10 system, feasibility study have been carried out. To find out the scale-up factor for designing the commercial scale EAF dust treatment process(capacity 50,000 ton EAF dust per year) entitled RAPID-50 system. The design and construction of RAPID-50 (RIST Arc Plasma Industrial Device) system for treating 50,000 ton of EAF dust per year is now undergoing. Overall plan for treating EAF dust generated in KOREA will be setup after successful operation (December, 2002) of RAPID-50 system.

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The Study on Real Scale Fire Test for Fire Growth of Office (사무용 공간의 화재 성장 예측을 위한 실물화재실험)

  • Kweon, Oh-Sang;Kim, Heung-Youl;Kim, Jung-Hyun
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2012.04a
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    • pp.278-281
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    • 2012
  • 사무공간의 화재성상을 예측하기 위해 화재하중 $25kg/m^2$ 값을 적용하여 $2.4(L){\times}3.6(W){\times}2.4(H)\;m$ 크기의 Mock-up 화재실험을 진행하였다. 화재실험은 실물화재실험 장비인 LSC(Large Scale Calorimeter)에서 실시하였으며, 열방출률 및 질량감소율을 측정하였다. 실물화재실험 시작 후 약 1110 초에 플래시오버가 발생하였으며, 최대 열발출률은 1241.1 KW로 측정되었고 질량은 초기 219 kg에서 102 kg로 감소하였다.

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Scaling Up Study of Exopolysaccharide Production through Mycelial Submerged Cultivation of Ganoderma lucidum (영지의 액체배양에 의한 세포외 다당 생산의 Scale Up 연구)

  • Lee, Hak-Su;Lee, Shin-Young
    • KSBB Journal
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    • v.24 no.3
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    • pp.303-311
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    • 2009
  • A scaling up study for the exopolysaccharide (EPS) production by submerged culture of Ganoderma lucidum was carried out in jar fermenter systems (2.6, 20 and 75 L) under bi-staged pH process. Profiles of dissolved oxygen (DO) and volumetric coefficient of oxygen transfer ($k_La$) as a function of operating variables (agitation speed and aeration rate) was investigated, and a correlation between $k_La$ and operating variables was analysed statistically. Under bi-staged pH process, no limitation of DO was observed at agitation speeds tested in the range of 200 and 600 rpm, and the highest EPS production was obtained at the level of DO of $40{\sim}80%$. From the regression analysis, the relation between $k_La$, gas velocity (Vs), stirrer speed (N) and impeller diameter (Di) could be expressed as : $$k_La=0.555{\times}Vs^{0.42}{\times}(N^3{\times}Di^2)^{0.33}\;(R^2=0.925,\;p<0.05)$$ It was found that under 2.6 L jar fermenter, the optimum agitation speed and aeration rate was 400 rpm and 1 vvm, respectively, obtaining the EPS production of 15.43 g/L. Under the submerged cultivation of G. lucidum in jar fermenters of $2.6{\sim}75\;L$, the similar EPS yields at each fermenter were achieved during scaling up based on $k_La$, and $k_La$ value for maximum EPS production was $85.4{\pm}26.70\;h^{-1}$.

Study on Multi-scale Unit Commitment Optimization in the Wind-Coal Intensive Power System

  • Ye, Xi;Qiao, Ying;Lu, Zongxiang;Min, Yong;Wang, Ningbo
    • Journal of Electrical Engineering and Technology
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    • v.8 no.6
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    • pp.1596-1604
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    • 2013
  • Coordinating operation between large-scale wind power and thermal units in multiple time scale is an important problem to keep power balance, especially for the power grids mainly made up of large coal-fired units. The paper proposes a novel operation mode of multi-scale unit commitment (abbr. UC) that includes mid-term UC and day-ahead UC, which can take full advantage of insufficient flexibility and improve wind power accommodation. First, we introduce the concepts of multi-scale UC and then illustrate the benefits of introducing mid-term UC to the wind-coal intensive grid. The paper then formulates the mid-term UC model, proposes operation performance indices and validates the optimal operation mode by simulation cases. Compared with day-ahead UC only, the multi-scale UC mode could reduce the total generation cost and improve the wind power net benefit by decreasing the coal-fired units' on/off operation. The simulation results also show that the maximum total generation benefit should be pursued rather than the wind power utilization rate in wind-coal intensive system.

Development of an R-based Spatial Downscaling Tool to Predict Fine Scale Information from Coarse Scale Satellite Products

  • Kwak, Geun-Ho;Park, No-Wook;Kyriakidis, Phaedon C.
    • Korean Journal of Remote Sensing
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    • v.34 no.1
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    • pp.89-99
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    • 2018
  • Spatial downscaling is often applied to coarse scale satellite products with high temporal resolution for environmental monitoring at a finer scale. An area-to-point regression kriging (ATPRK) algorithm is regarded as effective in that it combines regression modeling and residual correction with area-to-point kriging. However, an open source tool or package for ATPRK has not yet been developed. This paper describes the development and code organization of an R-based spatial downscaling tool, named R4ATPRK, for the implementation of ATPRK. R4ATPRK was developed using the R language and several R packages. A look-up table search and batch processing for computation of ATP kriging weights are employed to improve computational efficiency. An experiment on spatial downscaling of coarse scale land surface temperature products demonstrated that this tool could generate downscaling results in which overall variations in input coarse scale data were preserved and local details were also well captured. If computational efficiency can be further improved, and the tool is extended to include certain advanced procedures, R4ATPRK would be an effective tool for spatial downscaling of coarse scale satellite products.