• Title/Summary/Keyword: scale-up production

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ASSESSMENT OF A NEW DESIGN FOR A REACTOR CAVITY COOLING SYSTEM IN A VERY HIGH TEMPERATURE GAS-COOLED REACTOR

  • PARK GOON-CHERL;CHO YUN-JE;CHO HYOUNGKYU
    • Nuclear Engineering and Technology
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    • v.38 no.1
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    • pp.45-60
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    • 2006
  • Presently, the VHTGR (Very High Temperature Gas-cooled Reactor) is considered the most attractive candidate for a GEN-IV reactor to produce hydrogen, which will be a key resource for future energy production. A new concept for a reactor cavity cooling system (RCCS), a critical safety feature in the VHTGR, is proposed in the present study. The proposed RCCS consists of passive water pool and active air cooling systems. These are employed to overcome the poor cooling capability of the air-cooled RCCS and the complex cavity structures of the water-cooled RCCS. In order to estimate the licensibility of the proposed design, its performance and integrity were tested experimentally with a reduced-scale mock-up facility, as well as with a separate-effect test facility (SET) for the 1/4 water pool of the RCCS-SNU to examine the heat transfer and pressure drop and code capability. This paper presents the test results for SET and validation of MARS-GCR, a system code for the safety analysis of a HTGR. In addition, CFX5.7, a computational fluid dynamics code, was also used for the code-to-code benchmark of MARS-GCR. From the present experimental and numerical studies, the efficacy of MARS-GCR in application to determining the optimal design of complicated systems such as a RCCS and evaluation of their feasibility has been validated.

Operation Characteristics of Coal Syngas Production and DME Conversion Facilities (석탄 합성가스 제조 및 화학원료(DME) 전환설비의 운전 특성)

  • Chung, Seok-Woo;Kim, Mun-Hyun;Lee, Seung-Jong;Yun, Yong-Seung
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.83-86
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    • 2006
  • In this study, the syngas producing facility that consists of pulverized coal feeding/gasification and hot gas clean-up system was tested for Indonesian subbituminous coal. And the DME conversion facilities have been developed and tested for converting syngas to DME by reactions with catalysts. So, the entrained-bed slagging type pi lot scale coal gasifier was operated normally in the temperature range of $1,400{\sim}1,450^{\circ}C,\;7{\sim}8kg/cm^2$ pressure. And Roto middle coal produced syngas that has a composition of $36{\sim}38%$ CO, $14{\sim}16%\;H_2,\;and\;5{\sim}8%\;CO_2$. Particulates in syngas were 99.8% removed by metal filters. $H_2S$ composition in syngas was also desulfurized by the Fe chelate system to yield less than 0.1 ppm level. When the clean syngas $70{\sim}100 Nm^3/h$ was provided to DME conversion rector, normally operated in the temperature range of $230{\sim}250^{\circ}C$ and $60kg/cm^2$ pressure, 4.5% DME was yielded.

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Secretory Expression and Purification of the Recombinant Duck Interleukin-2 in Pichia pastoris

  • Du, Cuihong;Han, Long;Xiao, Anfeng;Cao, Minjie
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1264-1269
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    • 2011
  • Interleukin-2 (IL-2) is a vital cytokine secreted by activated T lymphocytes, and plays an important role in the regulation of cellular functions and immunity of animals. In this study, the recombinant duck IL-2 (rduIL-2) was secretory expressed in Pichia pastoris (P. pastoris). The recombinant P. pastoris strain was cultured in shake flasks and then scaled up in a 5.0-l bioreactor. The result showed that the maximal fresh-cell-weight of 594.1 g/l and the maximal $OD_{600}$ of 408 were achieved in the bioreactor. The rduIL-2 was purified by two steps of purification procedures, and approximately 311 mg of rduIL-2/L fermentation supernatant was obtained. SDS-PAGE showed that the purified rduIL-2 constituted a homogeneous band of ~16 kDa or ~14 kDa corresponding to the glycosylated or non-glycosylated duIL-2 protein in size, respectively. The bioactivity of rduIL-2 was determined by lymphocyte proliferation assay. The result indicated that the rduIL-2 greatly promoted the proliferation of ConA-stimulated lymphocytes in vitro. The P. pastoris expression system described here could provide promising, inexpensive, and large-scale production of the rduIL-2, which lays the foundation for development of novel immunoadjuvants to enhance both the immunity of ducks against various infectious pathogens and vaccine efficacy.

Operation Characteristics of the SBR Process with Electro-Flotation (EF) as Solids-liquid Separation Method (전해부상을 고액분리 방법으로 적용한 SBR 공정의 운전 특성)

  • Park, Minjeong;Choi, Younggyun
    • Journal of Korean Society on Water Environment
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    • v.24 no.3
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    • pp.340-344
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    • 2008
  • Electro-flotation (EF) was applied to a sequencing batch reactor process (SBR) in order to enhance solids-liquid separation. Solids-liquid separation was good enough in the SBR coupled with EF (EF-SBR) and it was possible to maintain the concentration of mixed liquor suspended solids (MLSS) high in the EF-SBR. Under moderate organic loading condition (COD loading rate: 6 g/day), control SBR (C-SBR) showed similar treatment efficiencies with the EF-SBR. Under high organic loading condition (COD loading rate: 9.6 g/day), the solids-liquid separation in the C-SBR was deteriorated due to proliferation of filamentous bulking organisms at high F/M ratio. However, the EF-SBR was operated stably and with the high MLSS concentration (above 4,000 mg/L) regardless of the organic loading conditions during overall operating period leading to the satisfactory effluent quality. Gas production rate of the electrodes was gradually decreased because of anodic corrosion and scale build-up at the surface of cathode. However it could be partially overcome by use of corrosion-proof electrode material (SUS-316 L) and by periodic current switching between the electrodes.

Effects of Carboxymethyl Chitosan on Yield and Whey Protein Loss in Cottage Cheese

  • Kim, Kyung-Tae;Kang, Ok-Ju
    • Preventive Nutrition and Food Science
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    • v.10 no.3
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    • pp.231-238
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    • 2005
  • A standard $1\%$ w/v solution of CM-chitosan made from squid pen was added to milk at levels of $0.5\sim3\%$ (v/v) to improve the yield and rheological properties of cottage cheese by whey protein retention. Cheese curd did not form at levels higher than $3\%$ (v/v) CM-chitosan standard solution. Yield and total protein of cottage cheese increased up to $2\%\;by\;11\;to\;42\%\;and\;17\;to\;38\%$ respectively, compared to control cheese. Whey protein losses were decreased by 11 to $42\%$ and thus accounted for all of the increase in yield. Anomalous results were obtained at the $0.8\%$ level, which neither improved yield or whey protein retention nor stabilized rheological parameters, and at the $0.5\%$ level, which improved yield and total protein without increasing whey protein retention. Elasticity and cohesiveness of CM-chitosan-containing cheese were generally improved and stabilized during storage. Monitoring of cheese chromaticity values for four weeks revealed a delay in the onset of yellowing in cheeses with CM-chitosan compared to the controls, while the concentration of added CM-chitosan had little influence on cheese chromaticity. The addition of CM-chitosan solution could be applied directly to industrial scale cottage cheese-making without the need for any modification of the production process.

Biological Synthesis of Genistein in Escherichia coli

  • Kim, Bong-Gyu
    • Journal of Microbiology and Biotechnology
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    • v.30 no.5
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    • pp.770-776
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    • 2020
  • Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.

Purification of Cephalosporin C Produced by Cephalosporium acrernoniurn (Cephalosporium acremonium 변이주가 생성하는 Cephalosporin C의 정제)

  • 이헌주;손영선;안동호;김현수;현형환
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.178-182
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    • 1992
  • For an industrial-scale purification and production of cephalosporin C from a culture broth of Ceplzalos#mium nmemonium CSA-2.8A3 mutant, ultrafiltration, column chromatography, reverse osmosis, and spray drying were empolyed. Above 90% of yield and high purity of cephalosporin C were obtained through WA-30, HP-20, XAD-2000 and SK-1B column chromatographies. Especially, in the tendom operation of the columns, the recovery yield was increased up to 96%. The purified cephalosporin C was stable at $4^{\circ}C$ and in acidic condition, while it was unstable at room temperature and in alkaline condition at pH above 8.0. Cephalosporin C powder or a final product prepared by spray drying contained 85.554 of sodium cephalosporin C, 6.3%' of water, 4.63% of free $Na^+$ ions. and traces of metal ions.

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Temperature Effect of the UASB Process for Treatment of Organic Waste (상향류식 혐기성 입상슬러지 공법의 유기폐수 처리 효율에 미치는 온도의 영향)

  • Park, Chul Hwi;Chung, Tai Hak
    • Journal of Korean Society of Water and Wastewater
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    • v.10 no.4
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    • pp.45-54
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    • 1996
  • Effects of temperature on the efficiency of the Upflow Anaerobic Sludge Blanket(UASB) process for treatment of wastewater from a starch and related products manufacturing industry were investigated using laboratory scale reactors equipped with two types of Gas-Solid Separator(GSS). Both fresh digested sludge and granular sludge stored nearly for one year at room temperature were good as a seeding material. The reactors seeded with aged granular sludge showed slow start-up, however, lowered activity at the initial period was recovered gradually. The GSS with an inner cylinder was proved to be effective in liquid-solid separation compared to the conventional type. Although the rate of organic removal and gas production per unit volatile suspended solids in the reactor reduced significantly as the temperature varied from 35 to $20^{\circ}C$, possibility of operation at low temperatures was shown as a result of gradual buildup of volatile suspended solids in the bed. Stable operation with a reduced efficiency was possible at a COD loading of $5-8kg/m^3/day$ at a temperature as low as $20^{\circ}C$.

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Improvement of Mechanical Properties of P/M Processed $2XXX Al-SiC_w$ Composites ($2XXX Al-SiC_w$ 복합재료의 분말야금 제조와 기계적 성질 향상 연구)

  • 신기삼
    • Journal of Powder Materials
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    • v.2 no.3
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    • pp.238-246
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    • 1995
  • The purpose of this study is to establish powder metallurgy (P/M) fabrication processes for high performance 2XXX Al composites reinforced with SiC whiskers. Rapidly solidified 2XXX Al powders produced by commercial atomization technique were mixed with SiC whiskers. The results of mixing processes indicated that fluidized zone mixing technique was considerably effective for the large scale production of the mixture of Al powders and whiskers. In order to consolidate these $Al-SiC_w$ mixtures into $Al-SiC_w$ composite billets, a vacuum hot press was set up, and hot processing variables were investigated. Using the hot pressing temperature of $620^{\circ}C$ under the pressure of 50 MPa, good quality $Al-SiC_w$ composite billets having relatively homogeneous microstructure and sound Al/sic interfacial bonding were obtained. Composite billets were then extruded to bars having relatively homogeneous microstructures at the extrusion temperature of 450~500$^{\circ}C$ under the extrusion pressure of 700~ 1000 MPa. Mechanical properties of the extruded bars were found to be comparable with those of the composite processed by Advanced Composite Materials Corp. To improve mechanical properties of the composites, elimination of coarse intermetallic compounds, uniform distribution of reinforcements, and minimization of whisker breakage are suggested.

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Treatment of Red Tide in Ocean Using Hydroxyl Radical

  • Zhitao Zhang;Mindong Bai;Xiyao Bai;Xue, Xiao-Hong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2004.05a
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    • pp.38-42
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    • 2004
  • A pilot-scale experiment for the treatment of red tide in the enclosure was done in sea area of Shandong Province, P. R. China on Aug. 25, 2002. With the method of strong dielectric barrier discharge in microgap, $O_2$in air and $H_2O$ in seawater are ionized and dissociated into large numbers of OHㆍradicals, and then dissolved into a part of seawater to form OHㆍsolution of high concentration. With OH' concentration of 0.68mg/L, the kill efficiencies of 29 kinds of red tide organisms such as Chaetoceros lorenzianus and so on reached 99.89%, in which the kill efficiencies of bacterium and vibrio were 100%, and that of Gonyaulax cysts and Prei. Cysts were up to 100%. At the same time, the content of chlorophyll-a was decreased into the lowest limit of test. DO saturation of seawater was greatly increased to 100% because the residual OHㆍradical was decomposed into $H_2O$ and $O_2$after 20 minutes, Therefore the treatment of red tide using OHㆍradicals is a kind of advanced oxidation technology, which realizes zero pollution, zero emission and zero residual in the process of the production of OHㆍradicals and the treatment of red tide.

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