Purification of Cephalosporin C Produced by Cephalosporium acrernoniurn

Cephalosporium acremonium 변이주가 생성하는 Cephalosporin C의 정제

For an industrial-scale purification and production of cephalosporin C from a culture broth of Ceplzalos#mium nmemonium CSA-2.8A3 mutant, ultrafiltration, column chromatography, reverse osmosis, and spray drying were empolyed. Above 90% of yield and high purity of cephalosporin C were obtained through WA-30, HP-20, XAD-2000 and SK-1B column chromatographies. Especially, in the tendom operation of the columns, the recovery yield was increased up to 96%. The purified cephalosporin C was stable at $4^{\circ}C$ and in acidic condition, while it was unstable at room temperature and in alkaline condition at pH above 8.0. Cephalosporin C powder or a final product prepared by spray drying contained 85.554 of sodium cephalosporin C, 6.3%' of water, 4.63% of free $Na^+$ ions. and traces of metal ions.

Cephalosporium acremonium CSA-2.8A3 변이주로 부터 생성된 cephaloporin C를 정제하여 산업적 이용을 위해 ultrafiltration으로 균체를 제거하고 수종의 수지공정을 통하여 소디움염 형태의 cephalosporin C를 정제하였으며 역삼투막에 의한 농축과 분무건조를 행하였다. WA-30, HO-20, XAD-2000, SK-1B column chromatography를 통해 90% 이상의 높은 수율과 순도를 얻을 수 있었고, 특히 상기 수지들을 직렬로 연결하여 사용합으로써 수율은 96%까지 올릴 수 있었다.