• Journal of Life Science
• /
• v.22 no.6
• /
• pp.703-712
• /
• 2012

Adeno-associated virus (AAV) has been considered to be a very safe and efficient gene delivery system. However, the major obstacles to therapeutic usage of AAV have been to achieve highly efficient and reproducible production processes, and also to develop a reliable quantifying method of various serotypes with a simple protocol. We compared the efficiency of the conventional production protocol of AAV2 and adenovirus (Ad) co-infection to that of a new method containing AAV2 infection followed by pHelper transfection. We tested HEK293 and 293T, and further examined the time-dependent changes of AAV2 production. The new method of AAV2 and pHelper DNA gave about ten times higher production efficiency than that of the conventional protocol. The highest production efficiency in 293T was achieved as $1.61{\times}10^5$ virus genomes (v.g.)/cell by the new method of 10 MOI of AAV2 infection and 5 days post-infection. This protocol of the highest efficiency was then applied to produce various AAV serotypes and showed the efficiencies higher than $10^5$ v.g./cell. Next, we designed the universal PCR primers of highly conserved regions for various AAV serotypes to develop a simple and reliable titration method. The universal primers could amplify all the tested AAV serotypes with similar sensitivities by ten molecular copies. Therefore, this pair of universal primers can be further utilized to detect AAV contaminants in therapeutic adenoviral vectors.

• Current Research on Agriculture and Life Sciences
• /
• v.33 no.1
• /
• pp.25-31
• /
• 2015

This study investigated the operation criteria of specialized complexes for horticultural production reflecting the farmers' preferences. First, the analysis of the communal activity included six factors: the group purchase of consumables for common activity, group purchase of the greenhouse apparatus, cooperative seed raising, use of a common air conditioning and heating system, cooperative shipping, and soil examination and certification system. The results of the Importance-Performance analysis can be summarized as follows. The factors requiring good management included the group purchase of consumables for common activity, group purchase of the greenhouse apparatus, and cooperative shipping. The factors with a lower priority included cooperative seed raising and the use of a common air conditioning and heating system. While the importance of the soil examination and certification system was low, the satisfaction was high, so this factor needs to be managed to avoid overkill. Second, the analysis of information exchange and education included six factors: production technique information, greenhouse facility management information, distribution-related information, production technique education, greenhouse facility management education, and distribution-related education. The results of the Importance-Performance analysis can be summarized as follows. The factor of production technique education was the most important determinant, plus the factors requiring good management included production technique information, greenhouse facility management information, and distribution-related information. The factors with a lower priority included greenhouse facility management education and distribution-related education. Therefore, to enhance productivity through facility modernization, the scaling up and creation of more specialized horticulture complexes are recommended as policy measures to gain export competitiveness. As the Korean government is expected to expand the scale of specialized horticulture complexes, the results of this paper can be widely utilized.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.6
• /
• pp.456-459
• /
• 2000

A pilot scale experiment was performed for a year to develop a two-phase anaerobic process for piggery wastewater treatment (COD: 6,000mg/L, BOD: 4,000mg/L, SS: 500mg/L, pH 8.4, alkalinity 6,000mg/L). The acidogenic reactor had a total volume of 3㎥, and the methanogenic reactor, an anaerobic up-flow sludge filter, combining a filter and a sludge bed, was also of total volume 3㎥(1.5㎥ of upper packing material). Temperatures of the acidogenic and methanogenic reactors kept at 20$^{\circ}C$ and 35$^{\circ}C$, respectively. When the pH of the acidogenic reactor was controlled at 6.0-7.0 with HCl, the COD removal efficiency increased from 50 to 80% over a period of six months, and as a result, the COD of the final effluent fell in the range of 1,000-1,500 mg/L. BOD removal efficiency over the same period was above 90%, and 300 to 400 mg/L was maintained in the final effluent. The average SS in the final effluent was 270 mg/L. The methane production was 0.32㎥ CH$_4$/kg COD(sub)removed and methane content of the methanogenic reactor was high value at 80-90%. When the pH of the acidogenic reactor was not controlled over the final two months, the pH reached 8.2 and acid conversion decreased compared with that of pH controlled, while COD removal was similar to the pH controlled operation. Without pH control, the methane content in the gas from methanogenic reactor improved to 90%, compared to 80% with pH control.

• Polymer(Korea)
• /
• v.29 no.6
• /
• pp.613-616
• /
• 2005

In this word as the first step to develop optical biosensors for a single cell level analysis, the preparation method of nano-scale polymer hydrogel spheres containing an enzyme was set up and the feasibility of the spheres as optical biosensors was investigated. The horseradish peroxidase (HRP) was encapsulated in the PEG hydrogel spheres by suspension photopolymerization, yielding spheres of the average size of 305 nm. After the polymerization, the incorporation and activity of HRP within the spheres were determined by the production of fluorescence resulted from the enzymatic reaction between HRP and $\H_{2}O_{2}$. The fluorescence emission response of the HRP-loaded PEG hydrogel spheres increased by nearly 300$\%$ as hydrogen peroxide concentration was changed from 0 to 11 nM in the presence of Amplex Red. The results suggest that the method to prepare the PEG hydrogel nanospheres containing an enzyme could be used for developing optical biosensors to measure various analytes in the very small samples like a single cell.

• Proceedings of the Membrane Society of Korea Conference
• /
• 2002.07a
• /
• pp.1-12
• /
• 2002

Desalination by reverse osmosis (RO), which first entered commercial use in the 1970s, was initially mainly used for treating brackish water. Technological progress led to the development of an RO membrane enabling single-pass seawater desalination. Toyobo succeeded in developing a single-pass seawater desalination RO module composed of hollow fiber type membranes made of cellulose triacetate in 1978, and then in 1979 began production of the first commercially available double-element module. This double-element module has many advantages suitable for seawater desalination. It has high chlorine tolerance and high salt rejection, derived from the properties of the membrane material, and it is highly resistant to fouling and scaling matters due to the unique flow pattern and fiber bundle configuration. These advantages help to explain why the Toyobo double-element module has been used so successfully at the many seawater desalination plants around the world. Since the 1980s, large plants capable of desalinating several tens of thousands of cubic meters a day have sprung up around the Mediterranean and In the Middle East. The Jeddah RO Phase I Plant, which has a capacity of 56, 800m$^3$/day, went into operation in 1989. In 1994, the same sized Phase II Plant came on stream, giving the plant a huge total capacity of 113, 600m$^3$/day. The plant constructor Mitsubishi Heavy Industries, Ltd. (MHI), and the RO membrane manufacturer Toyobo Co., Ltd. In 1998, the world's largest RO seawater desalination plant in operation, which has a capacity of 128, 000m$^3$/day and is run by Saudi Arabia's Saline Water Conversion Corporation (SWCC), went into operation at Yanbu. RO seawater desalination technology has thus already reached the stage of full-scale commercial use. In order to encourage its wider use, however, RO desalination needs to be made more economical by lowering construction and water treatment costs. Toyobo has therefore developed a new economical RO desalination system by a recovery ratio of 60% using a high-pressure module with a high product flow rate. In 2000, Toyobo high recovery membrane module was selected for the largest seawater desalination plant in Japan, which has a capacity of 50, 000m$^3$/day.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.11 no.3
• /
• pp.17-25
• /
• 2016

This study aims to empirically examine serial entrepreneurship in Korea. Using the ICT startups survey data, I compare serial entrepreneurs with their novice counterparts in terms of business behaviors and performances. I found that serial entrepreneurs are more likely than novice ones to co-found a business with other entrepreneurs, enter a market with relatively large assets and expand their business into overseas market. They tend to put more importance on large-scale production and expansion strategy than first-time entrepreneurs. They are more likely to cooperate with suppliers and customers but less likely with the governments. From their experience of founding and operating businesses, the findings suggest that serial entrepeneurs are more market-oriented and more capable of managing internal human relations and external network of supply chain. Consequently, serial entrepreneurs show stronger early financial performance than novice entrepreneurs especially when they team up to found a business.

• Korean journal of applied entomology
• /
• v.22 no.2 s.55
• /
• pp.84-97
• /
• 1983

Under the limited arable land, the enhancement of agricultural productivity is indispensable to provide the food demand which is concomitant with the rapid increase in population. From this viewpoint, the upbringing and dissemination of high-yielding varieties has been promoted continuously and several modifications in cultural practices, including heavy fertilization, dense planting, and early transplanting, also have been gradually developed. However these changes in cultivation have led to the increased outbreak of insect pests and diseases. And this unexpected results have accelerated the number and complexity of pesticides employed as well as their consumption. Even though pesticides are essential materials contributing to the steady production of agricultural crops, large scale consumption of them has given rise to several adverse impacts, such as mammalian hazard and/or environmental contamination. In this respect, recent development of new pesticides has been concentrated on 'safe pesticide', as it were, that has the highly selective properties without unfavorable side influences on other ecosystem. According to literature cited up to now, feasibilities of safe pesticide development would be summarized as two categories. One of them is the development of chemical pesticides, which include the molecular structure modification of established pesticides for increased safety and synthesis of new safe chemicals which can attack the vulnerable point of physio-ecological characteristics in insect pests and diseases. The other is the biological pesticides which comprise natural enemies and microorganisms to act selectively on confined insect pests and diseases, In addition, improvement of physico-chemical properties of available pesticide formulations would be one of the desirable means for safe pesticide development in view of efficacy enhancement and minimization of hazardous properties or safe pesticide development, various approaches are feasible and needed to study, however, long period and much financial outlay are necessary to develop a new item. And under the present situation in Korea, there are many difficulties for performing research on all the possible routes. Therefore, combined pesticides by the reasonable combination of already registered resticides evaluated as the fairly safe pesticides and safe formulation based on their physico-chemical properties would be developed primarily. And many efforts would be given gradually for the development of new chemical and biological pesticides.

• Journal of Families and Better Life
• /
• v.5 no.2
• /
• pp.45-64
• /
• 1987

In this study the phenomenon of commodity substitution of housework is approach in the contexts of economic system, patriachal family system and housework-related human resources. the objectives of this study are as follows: (1) To measure the level of commodity substitution of housework in large cities. (2)To investigate the factors influencing commodity substitution of housework. The samples were composed of 982 housewives dwelling in 6 large cities including Seoul. The statistics used for data analysis were frequency distribution, percetile. multiple regression analysis. The major findings are the following; (1) When the housework was divided into two subdomains , in the domain of food the purchase level was for below average while in the domain of clothes, the level was quite higher than average. This result hows that commodity substitution has become more common in the domain of clothes by mass production of clothes on a commercial scale. (2) the level of commodity substitution of housework was influenced by the family attribute variables such as housewife's age, family income. housewife's education, family types, housewife's brought-up regions, presence of sewing machine, employment status of housewife, by one patriarchal norm variable such as home-orientedness, and by tow human resource variables such as importance of housework and household tasks performance competnecy. Among variables house wife's age was the most influential one and such variables as family income, housewife's education, household task performance competency, importance of housework, and home-orientedness were also important. Considering that the influence of home-orientedness demonstrates the importance of patriarchy and that importance of housework and that performance competency also are determined by the patriarchal variables such as sex-role attitude and home-orientedness, we can conclude that Hartmann's theoretical approach with which the changes of housework were explained in the two contexts of economic system and family system is applied to the changes of housework in Korea.

• Proceedings of the KIEE Conference
• /
• 2011.07a
• /
• pp.2005-2006
• /
• 2011

Due to the advantages of small sizes, more I/O ports, etc., Ball Grid Array (BGA) has been used in the production of printed circuit board (PCB). However, BGA voids can degrade the performance of the board and cause failure. To automatically detect the voids in X-ray image, a novel blob filter that makes use of the local image gradient magnitude is proposed in this paper. The utilization of the local image gradient magnitude makes the proposed filter invariant to the image brightness, void shape, void position, and component interference. Furthermore, different sizes of box filters are employed to analyze the image in multi-scale, and as a result, the proposed blob filter is robust to void size. Experimental results show that the proposed method can obtain void detection accuracy up to 96.104% while keep low false ratio.

• Journal of Power System Engineering
• /
• v.16 no.6
• /
• pp.52-58
• /
• 2012

Roll forming process is one of the most widely used processes in the world for forming metals such as a furniture drawer guide or an up and down slide guide. It can manufacture goods of the uniform cross section on a large scale throughout the continuous processing. In the domestic design and manufacture, roll forming process for production is taking most of the method of 2-point under rail slide. However, this method is vulnerable to the structural strength and stiffness is not suitable for high load conditions. Therefore, through systematic study of high load, low noise 3-point ball type of under rail slide its own design and manufacturing technology is the need to build. In this paper, to make center member of 3-point ball-type under rail slide for the refrigerator doors, the roll forming modeling and simulation are performed. Tensile test is performed about SCP-1 1/2H for determine the mechanical properties of materials. Modeling and simulation of roll forming is used MSC.MARC software of a dedicated analysis program used by rigid plastic finite element method. Interference between the roll and the final shape are predicted from the results of the simulation.