• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 추계학술대회
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• pp.83-86
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• 2006

In this study, the syngas producing facility that consists of pulverized coal feeding/gasification and hot gas clean-up system was tested for Indonesian subbituminous coal. And the DME conversion facilities have been developed and tested for converting syngas to DME by reactions with catalysts. So, the entrained-bed slagging type pi lot scale coal gasifier was operated normally in the temperature range of $1,400{\sim}1,450^{\circ}C,\;7{\sim}8kg/cm^2$ pressure. And Roto middle coal produced syngas that has a composition of $36{\sim}38%$ CO, $14{\sim}16%\;H_2,\;and\;5{\sim}8%\;CO_2$. Particulates in syngas were 99.8% removed by metal filters. $H_2S$ composition in syngas was also desulfurized by the Fe chelate system to yield less than 0.1 ppm level. When the clean syngas $70{\sim}100 Nm^3/h$ was provided to DME conversion rector, normally operated in the temperature range of $230{\sim}250^{\circ}C$ and $60kg/cm^2$ pressure, 4.5% DME was yielded.

• Journal of Microbiology and Biotechnology
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• 제21권12호
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• pp.1264-1269
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• 2011

Interleukin-2 (IL-2) is a vital cytokine secreted by activated T lymphocytes, and plays an important role in the regulation of cellular functions and immunity of animals. In this study, the recombinant duck IL-2 (rduIL-2) was secretory expressed in Pichia pastoris (P. pastoris). The recombinant P. pastoris strain was cultured in shake flasks and then scaled up in a 5.0-l bioreactor. The result showed that the maximal fresh-cell-weight of 594.1 g/l and the maximal $OD_{600}$ of 408 were achieved in the bioreactor. The rduIL-2 was purified by two steps of purification procedures, and approximately 311 mg of rduIL-2/L fermentation supernatant was obtained. SDS-PAGE showed that the purified rduIL-2 constituted a homogeneous band of ~16 kDa or ~14 kDa corresponding to the glycosylated or non-glycosylated duIL-2 protein in size, respectively. The bioactivity of rduIL-2 was determined by lymphocyte proliferation assay. The result indicated that the rduIL-2 greatly promoted the proliferation of ConA-stimulated lymphocytes in vitro. The P. pastoris expression system described here could provide promising, inexpensive, and large-scale production of the rduIL-2, which lays the foundation for development of novel immunoadjuvants to enhance both the immunity of ducks against various infectious pathogens and vaccine efficacy.

• 한국물환경학회지
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• 제24권3호
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• pp.340-344
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• 2008

Electro-flotation (EF) was applied to a sequencing batch reactor process (SBR) in order to enhance solids-liquid separation. Solids-liquid separation was good enough in the SBR coupled with EF (EF-SBR) and it was possible to maintain the concentration of mixed liquor suspended solids (MLSS) high in the EF-SBR. Under moderate organic loading condition (COD loading rate: 6 g/day), control SBR (C-SBR) showed similar treatment efficiencies with the EF-SBR. Under high organic loading condition (COD loading rate: 9.6 g/day), the solids-liquid separation in the C-SBR was deteriorated due to proliferation of filamentous bulking organisms at high F/M ratio. However, the EF-SBR was operated stably and with the high MLSS concentration (above 4,000 mg/L) regardless of the organic loading conditions during overall operating period leading to the satisfactory effluent quality. Gas production rate of the electrodes was gradually decreased because of anodic corrosion and scale build-up at the surface of cathode. However it could be partially overcome by use of corrosion-proof electrode material (SUS-316 L) and by periodic current switching between the electrodes.

• Preventive Nutrition and Food Science
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• 제10권3호
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• pp.231-238
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• 2005

A standard $1\%$ w/v solution of CM-chitosan made from squid pen was added to milk at levels of $0.5\sim3\%$ (v/v) to improve the yield and rheological properties of cottage cheese by whey protein retention. Cheese curd did not form at levels higher than $3\%$ (v/v) CM-chitosan standard solution. Yield and total protein of cottage cheese increased up to $2\%\;by\;11\;to\;42\%\;and\;17\;to\;38\%$ respectively, compared to control cheese. Whey protein losses were decreased by 11 to $42\%$ and thus accounted for all of the increase in yield. Anomalous results were obtained at the $0.8\%$ level, which neither improved yield or whey protein retention nor stabilized rheological parameters, and at the $0.5\%$ level, which improved yield and total protein without increasing whey protein retention. Elasticity and cohesiveness of CM-chitosan-containing cheese were generally improved and stabilized during storage. Monitoring of cheese chromaticity values for four weeks revealed a delay in the onset of yellowing in cheeses with CM-chitosan compared to the controls, while the concentration of added CM-chitosan had little influence on cheese chromaticity. The addition of CM-chitosan solution could be applied directly to industrial scale cottage cheese-making without the need for any modification of the production process.

• Journal of Microbiology and Biotechnology
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• 제30권5호
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• pp.770-776
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• 2020

Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.

• 한국미생물·생명공학회지
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• 제20권2호
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• pp.178-182
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• 1992

Cephalosporium acremonium CSA-2.8A3 변이주로 부터 생성된 cephaloporin C를 정제하여 산업적 이용을 위해 ultrafiltration으로 균체를 제거하고 수종의 수지공정을 통하여 소디움염 형태의 cephalosporin C를 정제하였으며 역삼투막에 의한 농축과 분무건조를 행하였다. WA-30, HO-20, XAD-2000, SK-1B column chromatography를 통해 90% 이상의 높은 수율과 순도를 얻을 수 있었고, 특히 상기 수지들을 직렬로 연결하여 사용합으로써 수율은 96%까지 올릴 수 있었다.

• 상하수도학회지
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• 제10권4호
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• pp.45-54
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• 1996

Effects of temperature on the efficiency of the Upflow Anaerobic Sludge Blanket(UASB) process for treatment of wastewater from a starch and related products manufacturing industry were investigated using laboratory scale reactors equipped with two types of Gas-Solid Separator(GSS). Both fresh digested sludge and granular sludge stored nearly for one year at room temperature were good as a seeding material. The reactors seeded with aged granular sludge showed slow start-up, however, lowered activity at the initial period was recovered gradually. The GSS with an inner cylinder was proved to be effective in liquid-solid separation compared to the conventional type. Although the rate of organic removal and gas production per unit volatile suspended solids in the reactor reduced significantly as the temperature varied from 35 to $20^{\circ}C$, possibility of operation at low temperatures was shown as a result of gradual buildup of volatile suspended solids in the bed. Stable operation with a reduced efficiency was possible at a COD loading of $5-8kg/m^3/day$ at a temperature as low as $20^{\circ}C$.

• 한국분말재료학회지
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• 제2권3호
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• pp.238-246
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• 1995

The purpose of this study is to establish powder metallurgy (P/M) fabrication processes for high performance 2XXX Al composites reinforced with SiC whiskers. Rapidly solidified 2XXX Al powders produced by commercial atomization technique were mixed with SiC whiskers. The results of mixing processes indicated that fluidized zone mixing technique was considerably effective for the large scale production of the mixture of Al powders and whiskers. In order to consolidate these $Al-SiC_w$ mixtures into $Al-SiC_w$ composite billets, a vacuum hot press was set up, and hot processing variables were investigated. Using the hot pressing temperature of $620^{\circ}C$ under the pressure of 50 MPa, good quality $Al-SiC_w$ composite billets having relatively homogeneous microstructure and sound Al/sic interfacial bonding were obtained. Composite billets were then extruded to bars having relatively homogeneous microstructures at the extrusion temperature of 450~500$^{\circ}C$ under the extrusion pressure of 700~ 1000 MPa. Mechanical properties of the extruded bars were found to be comparable with those of the composite processed by Advanced Composite Materials Corp. To improve mechanical properties of the composites, elimination of coarse intermetallic compounds, uniform distribution of reinforcements, and minimization of whisker breakage are suggested.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2004년도 SMICS 2004 International Symposium on Maritime and Communication Sciences
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• pp.38-42
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• 2004

A pilot-scale experiment for the treatment of red tide in the enclosure was done in sea area of Shandong Province, P. R. China on Aug. 25, 2002. With the method of strong dielectric barrier discharge in microgap, $O_2$in air and $H_2O$ in seawater are ionized and dissociated into large numbers of OHㆍradicals, and then dissolved into a part of seawater to form OHㆍsolution of high concentration. With OH' concentration of 0.68mg/L, the kill efficiencies of 29 kinds of red tide organisms such as Chaetoceros lorenzianus and so on reached 99.89%, in which the kill efficiencies of bacterium and vibrio were 100%, and that of Gonyaulax cysts and Prei. Cysts were up to 100%. At the same time, the content of chlorophyll-a was decreased into the lowest limit of test. DO saturation of seawater was greatly increased to 100% because the residual OHㆍradical was decomposed into $H_2O$ and $O_2$after 20 minutes, Therefore the treatment of red tide using OHㆍradicals is a kind of advanced oxidation technology, which realizes zero pollution, zero emission and zero residual in the process of the production of OHㆍradicals and the treatment of red tide.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.291-291
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• 2010

Organic solar cells have attracted much interest due to the potential advantage of the lightness, simple solution processing and flexibility. Until recently, the focus of organic solar cells research has been on optimization of material processing to improve the power conversion efficiency. However, area scaling is an important position for alternative to the market dominating solar cells. Spray deposition technologies have advantage of less material wastage and possibility of large scale photoactive area coating when compared with spin coating process. We investigated the performance of organic solar cells as a function of active area using two types of deposition process. The commonly used process is spin coating which can be fabricated organic materials deposition for devices. Spray deposition process compare with spin coating for large-area organic solar cells. The spray deposition organic layer shows excellent performance up to the active area of $4\;cm^2$ with the PCE of ~3.0 % under AM.1.5 simulated illumination with an intensity of $100mW/cm^2$. This indicates that the spray deposition process can be used as a mass production process for evaluating large-area organic solar cells.