• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.130-136
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• 2021

Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.

• Bulletin of the Korean Chemical Society
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• v.18 no.3
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• pp.287-291
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• 1997

The photochemical reaction of aqueous dichloromethane in the absence (saturated with argon) and the presence of O2 (saturated with air or oxygen) has been investigated using 184.9 nm UV light. The irradiation of the solution causes the formation of 1,2-dichloroethane, 1,1,2-trichloroethane, 1,1,2,2-tetrachloroethane and chloride ion. The initial quantum yield of the products in the absence of oxygen was determined to be 8.6 × 10-3, 7.3 × 10-3, 4.4 × 10-3 and 2.3 × 10-2, respectively. In addition to these main products, small amounts of 1,2,3-trichloropropane, 1,1,2,2,3-pentachloropropane, 1,1,2,3,3-pentachloropropane, 1,3-dichloropropane and 1,1,2,2,3,3-hexachloropropane were detected. In the presence of oxygen, hydrogen peroxide was also detected along with the products listed above. With increasing the concentration of oxygen, while formation of the chlorinated organic products diminished, formation of chloride ion increased. Probable reaction mechanisms for the photochemical reaction were presented on the basis of products analysis.

• Journal of the Korean Chemical Society
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• v.38 no.4
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• pp.271-275
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• 1994

The photochemical reaction of aqueous ethanol saturated with argon and carbon monoxide has been investigated using 184.9 nm UV light. The photochemical reaction of $1{\times}10^{-2}$ M aqueous ethanol saturated with argon results in the formation on the acetaldehyde and 2,3-butanediol. The irradiation of the solution saturated with carbon monoxide causes the formation of carbonylation and carboxylation products such as ${\alpha}$-hydroxypropionaldehyde, formaldehyde, glyoxal, formic acid, oxalic acid and glyoxylic acid in addition to above two products. But in the case of concentrated ethanol solutions, the irradiation did not give carbonylation and carboxylation products. The initial quantum yields of the products were determined and probable mechanisms for the reaction were presented on the basis of product analysis.

• Bulletin of the Korean Chemical Society
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• v.20 no.6
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• pp.683-688
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• 1999

In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet. This suggests the presence of two backbone conformations, the exchange between which is slow on an NMR time scale. The origin of the two conformations has been investigated in this work using saturated 1,2-diacyl-sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.11
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• pp.1363-1370
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• 1999

A study of scaling and heat transfer has been carried out for a vertical tube evaporator in which $CaSO_4$ saturated water flows upward. Experimental apparatus including vacuum chambers and heaters has been designed and manufactured to study scaling phenomena for three different pressures(1atm, 0.27atm and 0.16atm). Overall heat transfer coefficients have been measured and shown to decrease with time as scaling proceeds. After the end of experiments, the vertical tube has been cut to measure the thickness of scale at different heights. Below the height where the bulk fluid temperature does not reach saturated temperature, the thickness of scale increases, however, beyond that height occurring saturated condition, the thickness does not vary much or even decreases a little. The measured fouling resistances also support these variations of scale thickness.

• Journal of Environmental Science International
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• v.1 no.2
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• pp.105.2-117
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• 1992

The formation mechanism of the vortex streets in the lee of the mountain Is Investigated by a three-dimensional numerical model. The model is based upon the hydrostatic Boussinesq equations in which the vertical turbulent momentum flux is estimated by a turbulence parameterization scheme, but the horizontal viscosity is assumed to be constant. The results show that Karman vortex streets can form even without surface friction in a constant ambient flow with uniform stratification. The vortex formation is related to breaking of the mountain wave, which depends on the Froude number (Fr). In the case of a three-dimensional bell-shaped mountain, the wave breaking occurs when Fr is less than about 0.8, while a barman vortex forms when Fr is less than about 0.22. Vortex formation also depends on Reynolds number, which is estimated from the horizontal diffusivity. The vortex formation can be explained by the wave saturation theory given by Lindzen (1981) with some modification. Simulations in this study show that in the case of Karman vortex formation the momentum flux in the lower level is much larger than the saturated momentum flux, whereas it is almost equal to the saturated momentum at the upper levels as expected from the saturation theory. As a result, large flux divergence is produced in the lower layer, the mean flow is decelerated behind the mountain, and the horizontal wind shear forms between unmodified ambient wind. The momentum exchange between the mean flow and the mountain wave is produced by the turbulence within a breaking wave. From the result, well developed vortices like Karman vortex can be formed. . The results of the momentum budget calculated by the hydrostatic model are almost the same as nonhydrostatic results as long as horizontal scale of the mountain is 10 km. A well developed barman vortex similar to the hydrostatic one was simulated in the nonhydrostatic case. Therefore, we conclude that the hydrostatic assumption is adequate to investigate the origin of the Km8n vortex from the viewpoint of wave breaking.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.1 no.2
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• pp.105-117
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• 1997

The formation mechanism of the vortex streets in the lee of the mountain is investigated by a three-dimensional numerical model. The model is based upon the hydrostatic Boussinesq equations in which the vertical turbulent momentum flux is estimated by a turbulence parameterization scheme, but the horizontal viscosity is assumed to be constant. The results show that Karman vortex streets can form even without surface friction in a constant ambient flow with uniform stratification. The vortex formation is related to breaking of the mountain wave, which depends on the Froude number (Fr). In the case of a three-dimensional bell-shaped mountain, the wave breaking occurs when Fr is less than about 0.8, while a Karman vortex forms when Fr is less than about 0.22. Vortex formation also depends on Reynolds number, which is estimated from the horizontal diffusivity. The vortex formation can be explained by the wave saturation theory given by Lindzen (1981) with some modification. Simulations in this study show that in the case of Karman vortex formation the momentum flux in the lower level is much larger than the saturated momentum flux whereas it is almost equal to the saturated momentum at the upper levels as expected from the saturation theory. As a result, large flux divergence is produced in the lower layer, the mean flow is decelerated behind the mountain, and the horizontal wind shear forms between unmodified ambient wind. The momentum exchange between the mean flow and the mountain wave is produced by the turbulence within a breaking wave. From the result, well developed vortices like Karman vortex can be formed. The results of the momentum budget calculated by the hydrostatic model are almost the same as nonhydrostatic results as long as horizontal scale of the mountain is 10 km. A well developed Karman vortex similar to the hydrostatic one was simulated in the nonhydrostatic case. Therefore, we conclude that the hydrostatic assumption is adequate to investigate the origin of the Karman vortex from the viewpoint of wave breaking.

• Corrosion Science and Technology
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• v.7 no.1
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• pp.22-26
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• 2008

Formation of protective iron carbonate films in $CO_2$ corrosion can reduce corrosion rate substantially and the effects have been incorporated in various prediction models. The $CO_2$ corrosion with the presence of free acetic acid is known to increase corrosion rate below scaling temperature. The possible interaction between the formation of iron acetate and iron carbonate films can affect the protectiveness of the film. The study is done using 3% NaCl solution under stagnant $CO_2$ -saturated condition at the scaling temperatures at various pH values and HAC concentrations. The result show that the presence of HAc does not affect the formation of protective iron carbonate film but delays the attainment of protective iron carbonate due to a possibility of solubilising of ferrous ions and thinning of the films.

• 한국지구물리탐사학회:학술대회논문집
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• 2005.09a
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• pp.55-71
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• 2005

Jeju Island is mainly composed of basaltic lava flows and subordinate amounts of volcaniclastic sedimentary rocks. Jeju Province operates the monitoring wells for seawater intrusion problems around Jeju Island to evaluate of groundwater resources in coastal area. Various surveys and monitoring have been performed in boreholes, and also conventional geophysical well loggings conducted to identify basalt sequences and assess seawater intrusion problems. Various conventional geophysical well logs, including radioactive logs, electrical log, caliper log, and temperature and conductivity log and heat-pulse flowmeter log were obtained in 29 boreholes. The results of geophysical well loggings for saturated rocks are interesting and consistent. Natural gamma logs are useful in basalt sequences to sedimentary interbeds, unconsolidated U formation, and seoguipo formation with higher natural gamma log regardless of saturated or unsaturated basalts. Neutron logs are very effective to discriminate among individual lava flows, flow breaks, and sedimentary interbeds in saturated formation. In hyalocastite, porosity is high and resistivity is low, and we think that hyalocastite is a major pathway of fluid flow. In trachybasalt, porosity has a wide range and resistivity is high. In sedimentary interbeds, unconsolidated U formation and seoguipo formation, porosity is high and resistivity is low. The temperature logs in eastern area in Jeju are useful to interpret the hydrogeological unit and evaluate seawater intrusion in Suan area.

• Journal of Photoscience
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• v.6 no.4
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• pp.177-181
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• 1999

The photochemical transformation of carbon monoxide in aqueous ammonia solution has been investigated at 25${\pm}$0.1$^{\circ}C$ using 184.9 nm UV light. Amination and carbonylation processes were carried out by irradiating the aqueous ammonia solution saturated with carbon monoxide, and the formation of formamide, urea, hexamethylenetetramine, formaldehyde, glyoxal and hydrazine was observed. The formation of hydrazine was affected by the presence of ammonia, and the formation of carbonyl compounds such as formaldehyde and glyoxal was influenced by the presence of carbon monoxide. The formation of formamide, urea and hexamethylenetetramine was affected by both ammonia and carbon monoxide. The initial quantum yields of the products were determined and probable mechanisms for the photochemical reaction were presented on the basis of product analysis.