• 대한한방내과학회지
• /
• 제19권1호
• /
• pp.221-246
• /
• 1998

The aim of this experimental study was to evaluate the anti-tumor effects of Chungsimbohyeltang through investigation about viability of tumor cell by MTT assay, survival period of mice transplanted with L-1210 cells, growth inhibition on the tumor cell, body weight variation in mice transplanted with sarcoma-180 cells and its immunomodulatory effects through the investigation on delayed type hyper-sensitivity, the hemagglutinin and hemolysin titers for humoral immune response, the appearance of rosette forming cells for cell-mediated immune response, the natural killer cell activity, the transformation of lymphocyte, the productivity of Interleukin-2 and phagocytic index K was performed in immune-depressed ICR mice induced by methotrexate treatments. The results were as follows ; 1. $IC_{50}$ of Chungsimbohyeltang treated group was 5.85mg/ml in SNU-C4 cell, 1.38mg/ml in SNU-396 cell, 0.21mg/ml in SNU-1 cell, so it had low anti-tumor activity. 2. The both groups of Chungsimbohyeltang extract 10mg/kg and Chungsimbohyeltang extract 20mg/kg had no toxicity and the group of Chungsimbohyeltang 20mg/kg which was shown 120% in ILS had the effect of life prolongation in mice transplanted with L-1210 cells. 3. In the growth inhibition on the tumor cells, only the group of Chungsimbohyeltang extract 20mg/kg was noted and in the weight variation in mice transplanted with sarcoma-180 cells, both groups of Chungsimbohyeltang extract had a significant effect. 4. In the delayed type hypersensitivity and appearance of rosette forming cells, both groups of Chungsimbohyeltang extract didn't have any significant effect. 5. The hemagglutinin titers was slightly increased with no significance, and the hemolysin titers was significantly increased in the only group of Chungsimbohyeltang extract 20mg/kg. 6. The natural killer cell activity of the Chungsimbohyeltang extract groups was significantly increased in the ratio of 100:1 of effector and target cells, but it was not significantly increased in the ratio of 50:1, 10:1. 7. The transformation of lymphocyte and the productivity of Interleukin-2 were increased significantly and in dose-dependent manner in both group of Chungsimbohyeltang extract. 8. The phagocytic effect of macropage was significantly increased in both groups of Chungsimbohyeltang extract. Considering the results above, we could conclude that Chungsimbohyeltang have an indirect anti-tumor effect through the modulation of immunme response, although it had not toxicity on the tumor cell it self.

• Archives of Pharmacal Research
• /
• 제2권2호
• /
• pp.145-151
• /
• 1979

The carpophores of three Korean mushrooms, Coriolus versicolor, Pleurotus ostreatus and Lentinus edodes were respectively extracted with hot water and the extracts were dialyzed through Visking tube. They were found to exert an antitumor activity against sarcoma-180 implanted in mice. Especially, the inhibition ratio of the extract of Coriolus versicolor (100 mg/ Kg.i.p) was almost 100% . But all the extracts did not affect the growth of leukemia L517Y cells in vitro. Therefore these facts indicate that the extracts appear to stimulate cell-mediated immunity.

• 대한한의학방제학회지
• /
• 제5권1호
• /
• pp.147-168
• /
• 1997

Tish study was carried out to evaluate the possible therapeutic or antitumoral effects of Takrisodokyeum extract against tumor, and immunomodulatory effect. Some kinds of tumor were induced by the typical application of 3-methylcholanthrene (MCA) or by the implantation(s.c) of malignant tumor cells such as leukemia cells(3LL cells) or sarcoma cells(S-I80 and Fas II cells). Treatment of the Takrisodokyeum water-extract(daily 1mg mouse, i.p.) was continued for 7 days prior to tumor induction and after that the treatment was lasted for 15 days. Against squamous cell carcinoma induced by MCA, Takrisodokyeum decreased not only the frequency of tumor production but also the number and the weight of tumors per tumor bearing mice (TBM). Takrisodokyeum also significantly suppressed the development of 3LLcell and S-180 cell by frequency and their size, and some developed tumors were regressed by the continuous treatment of Takrisodokyeum extract into TBM. However, when tumor was induced by FsaII cell-implantation, the growth of implanted cells in mice was delayed by the water extract of Takrisodokyeum until day 7 and then rapid growth ensued. In vitro, treatment of Takrisodokyeum extract had no effect on the growth of some kind of cell lines such as FsaII, A-131 strain but significantly inhibited the proliferation of 3LL, S-180 cells. Takrisodokyeum also stimulated the migrative ability of leucocyte, the MIF and IL 2-production of T lymphocytes, but not IL 6 production of B cells. Takrisodokyeum enhanced Arthus reaction and DTH to sheep erythrocytes, and NK cells activities. These results demonstrated that Takrisodokyeum extract different results according to the type of tumor cells. And these results also suggested that antitumor effect of Takrisodokyeum might be chiefly due to nonspecific enhancement of NK cell activities and cell-mediated immune responses.

• 대한한방종양학회지
• /
• 제3권1호
• /
• pp.67-84
• /
• 1997

Bujeonghangamtang(扶正抗癌湯) has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate the possible therapeutic or antitumoral effects of Bujeonghangamtang extract against tumor, and to carry out some mechanisms responsible for its effect. Some kinds of tumor were induced by the typical application of 3-methylcholanthrene. (MCA) or by the implantation(s.c) of malignant tumor cells such as leukemia cells(3LL cells) or sarcoma cells(Sl80 cells). Treatment of the Bujeonghangamtang water-extract (dailly 1mg／mouse, i. p.) was continued for 7 days prior to tumor induction and after that the treatment was lasted for 20 days. Against squamous cell carcinoma induced by MCA, Bujeonghangamtang decreased not only the frequency of tumor production but also the number and the weight of tumors per tumor bearing mice (TBM). Bujeonghangamtang also significantly suppressed the development of 3LL cell and S180 cell-implanted tumors in occurrence-frequency and their size, and some developed tumors were regressed by the continuous treatment of Bujeonghangamtang extract into TBM. In vitro, treatment of Bujeonghangamtang extract had no effect on the growth of some kinds of cell line such as FsaII, A431 strain but significantly inhibited the proliferation of 3LL, S180 cells and augmented the DNA synthesis of mitogen-activated lymphocytes. Bujeonghangamtang also stimulated the migrative ability of leukocyte, the MIF and IL-2 production of T lymphocytes, but not IL 6 production of B cells. Bujeonghangamtang-administration to mice enhanced NK cells activities. These results demonstrated that Bujeonghangamtang extract exhibited a significant prophylactic benefits against tumors and its antitumor activity was manifested depending on the type of tumor cells. And these results also suggested that effect of Bujeonghangamtang might be chiefly due to nonspecific enhancement of NK cell activities and cell-mediated immune responses.

• 한국식품영양과학회지
• /
• 제33권3호
• /
• pp.469-474
• /
• 2004

본 연구에 사용한 아라비녹실란화합물은 미강에서 추출한 헤미셀룰로즈에 표고버섯균사체 배양물에서 분리한 효소를 작용시켜 생성된 식물성다당류이며, PSP(polysaccharide peptide)는 담자균류의 일종인 Coliolus versicocor Sov-1의 균사체에서 추출 정제하여 얻은 단백다당체 성분이다. 이들에 대한 면역세포 활성화 효과, 항암 효과 및 알레르기 억제효과 등에 관한 실험 결과는 다음과 같다. 비장세포의 B cell과 T cell에 arabinoxylane과 PSP의 항체생성과 세포활성능을 조사한 결과 PFC형 성은 10 ∼ 15%, RFC검출은 10 ∼ 30% 증가하였다 복강침출세포로부터 대식세포의 탐식능 효과는 대조군에 비하여 PSP는 약 18%, arabinoxylane은 22% 정도 증가하였다. S-180 고형암에 의한 항암실험에서 arabinoxylane은 66.2%(p<0.01), PSP는 49.0%(p<0.05)의 억제 효과를 확인할 수 있었다. 복수암에 의한 수명 연장효과는 PSP, arabinoxylane이 대조군에 비해 다소의 수명연장효과가 나타났으며, PSP가 아라비녹실란에 비해 약간의 높은 수명연장효과가 나타났다. 항알레르기 효과를 보기 위한 PCA 역가를 측정한 결과는 대조군이 80인데 비해 PSP군은 40, arabinoxylane군은 20으로 대조군에 비하여 다소 경감되었으며, arabinoxylane군이 다소 우수한 결과를 보였다. 혈중 histamine 함량도 PCA와 유사한 결과로 arabinoxylane군과 PSP군이 히스타민 함량을 다소 감소시키는 경향을 보였다.

• 한방안이비인후피부과학회지
• /
• 제21권1호
• /
• pp.26-37
• /
• 2008

Objective : The present study was carried out to investigate the effects of NaisoOkseol-Tang (NOT) on solid tumor in mice in terms of immune-potentiating and direct cytotoxic action of NOT in vitro and vivo study. Methods: The present author investigated thymocyte proliferation and NO production to confirm immune-potentiating activity of NOT and also investigated body/tumor weight and survival rates in tumor bearing mice. In this study, administration of NOT decreased tumor/weight ratio significantly, and prolonged survival duration compared to non-treated control. Treatment with NOT also elevated proliferation rates of thymocytes isolated from tumor bearing mice. Results: In vitro study, treatment with NOT suppressed proliferation rate of Sarcoma 180 (S-180) cells. Contrary, treatment with NOT elevated proliferation rate of thymocytes. These results were co-related with in vivo study. In addition, NO production from abdominal macrophages isolated from normal mice was not affected by treatment with NOT. Conclusion : NOT is useful to treat patient with solid tumor, because NOT has direct toxic action for tumor cell and immune-potentiating action for T cells.

• 한국균학회지
• /
• 제46권3호
• /
• pp.295-306
• /
• 2018

본 연구에서는 국내산 50% 에탄올 복령추출물(KPE)과 중국산 50% 에탄올 복령추출물(CPE)의 약리활성 비교를 위하여 전 지방세포주인 3T3-L1 세포주와 암세포주인 Sarcoma 180 세포주를 이용하여 지방축적 억제활성과 항암활성을 비교하였다. 또한 노지재배 및 시설재배의 1년산 복령과 2년산 복령의 50% 에탄올 복령추출물(LPE, FPE)에 대해서도 50% 항암억제활성($IC_{50}$)로 중국산 복령과 비교하였다. 추가적으로 현재 시중에서 유통되고 CPE와 KPE의 안전성 자료를 획득하고자 단회 경구 독성시험을 실시하였다. CPE와 KPE를 3T3-L1 세포에 처리 후 48시간 이후에 대조군과 비교 시 지방축적 억제를 보였으나(p< 0.05), CPE와 KPE 사이에서는 지방축적 억제활성의 차이가 없었다. Sarcoma 180에 대한 CPE와 KPE의 항암활성을 비교할 때 시험한 농도에서 유사한 활성을 보였지만 $300{\mu}g/mL$의 고농도에서는 KPE가 CPE보다 높은 항암활성을 보였다(p < 0.05). 중국산 복령을 대체하고자 인공재배에서 생산된 노지재배 복령(LPE)과 시설재배 복령(FPE)의 항암활성 비교에서 CPE의 항암활성보다 높게 나타났다(p < 0.05). 단회 독성시험에서 CPE와 KPE의 $LD_{50}$(반수치사량)은 2,000 mg/kg 이상으로 추정되었다. 위의 결과를 종합 시 암세포주인 Sarcoma 180 세포주를 이용한 항암활성측정법은 중국산과 국내산의 원산지 검증의 생물활성검증의 보조수단으로 활용이 가능할 것으로 기대되었다. 국내에서 인공재배 되는 노지재배 복령 및 시설재배 복령은 중국산 복령을 대체하여 수입 감소의 효과를 기대할 수 있으며 지방축적 억제활성 및 항암활성의 기능성 원료로 활용이 가능할 것으로 생각되었다.

• Toxicological Research
• /
• 제23권3호
• /
• pp.253-261
• /
• 2007

In this study, we investigated the in vitro anti-obesity, anti-cancer activity and single oral dose toxicity of Inonotus xeranticus extracted by methanol (INXM) or ethyl acetate (INXE). In order to investigate anti-obestity effect of Inonotus xeranticus extracts, the 3T3-L1 cells were treated with these extracts at various concentrations(1, 10, 100 and $300{\mu}g/ml$). It was observed that 3T3-L1 cells treated with $100{\mu}g/ml$ of Inonotus obliquus ethyl acetate extract (INOE), INXM and INXE, in the absence of differentiation cocktail (0.5mM isobutylmethylxanthine (IBMX) $1{\mu}M$ dexamethasone, $1{\mu}M$ insulin), differentiated at a rate of 78.5, 80.9, and 76.4% respectively. Differentiation rates of 86.6% and 83.4% were observed in 3T3-L1 cells which were treated with differentiation cocktail at $100{\mu}g/ml$ of INXM and INXE, respectively. The anti-cancer effect of Inonotus xeranticus extracts was investigated using a method of sulforhodamine B in sarcoma 180 cell line. The cells were treated with these extracts (1, 10, 100 and $300{\mu}g/ml$) for 48 hours. The growth of cells which were treated with $300{\mu}g/ml$ of INXM was inhibited by 80.1%. The growth of sarcoma 180 cells which were treated with 100 and $300{\mu}g/ml$ of INXE was inhibited by 74.7% and 64.5%, respectively. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs, body weight gains, and feed and water consumptions. The results indicated that Inonotus xeranticus extracts did not show any toxic effects at 2,000mg/kg in mice, and the $LD_{50}$ of these extracts was found to be higher than 2,000 mg/kg in this experiment. From the above results, Inonotus xeranticus methanol and ethyl acetate extracts might have useful clinical applications in the management of cancer and obesity and may also be useful as a medicinal food.

• 생약학회지
• /
• 제17권1호
• /
• pp.39-48
• /
• 1986

To find antitumor components in the shake-cultured mycelia of Volvariella bombycina, the mycelia were extracted with hot water. After the extract was dialyzed and freeze-dried, a protein-polysaccharide fraction was obtained and examined for antitumor activity against the solid form of sarcoma 180 in ICR mice. It showed 60.3% inhibition ratio at a dose of 20mg/kg/day for 10 days. It was found to consist of a polysaccharide moiety and a protein moiety. After gel filtration on Sepharose 4B, Fraction B was obtained and showed the highest inhibition ratio of 71.1%. When the antitumor component was examined for immunopotentiating activity, it was found to increase the macrophage accumulation in the peritoneal cavity as well as the antibody production of the spleen cells of the mice.

• 한국임상수의학회:학술대회논문집
• /
• 한국임상수의학회 2006년도 추계학술대회 및 정기총회
• /
• pp.74-75
• /
• 2006