• BMB Reports
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• v.40 no.1
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• pp.58-64
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• 2007

Similar to the other known structures of Bacillus thuringiensis Cry $\delta$-endotoxins, the crystal structure of the 65-kDa activated Cry4Ba toxin comprises three domains which are, from the N- to C-terminus, a bundle of $\alpha$-helices, a three-$\beta$-sheet domain, and a $\beta$-sandwich. To investigate the properties of the C-terminal domain III in isolation from the rest of the toxin, the cloned Cry4Ba-domain III was over-expressed as a 21-kDa soluble protein in Escherichia coli, which cross-reacted with anti-Cry4Ba domain III monoclonal antibody. A highly-purified domain III was obtained in a monomeric form by ion-exchange and size-exclusion FPLC. Circular dichroism spectroscopy indicated that the isolated domain III fragment distinctly exists as a $\beta$-sheet structure, corresponding to the domain III structure embodied in the Cry4Ba crystal structure. In vitro binding analysis via immuno-histochemical assay revealed that the Cry4Ba-domain III protein was able to bind to the apical microvilli of the susceptible Stegomyia aegypti larval midguts, albeit at lower-binding activity when compared with the full-length active toxin. These results demonstrate for the first time that the C-terminal domain III of the Cry4Ba mosquito-larvicidal protein, which can be isolated as a native folded monomer, conceivably participates in toxin-receptor recognition.

• Applied Science and Convergence Technology
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• v.24 no.5
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• pp.178-183
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• 2015

Optical, electrical and structural properties of VOx/ZnO/VOx thin film are studied. The VOx/ZnO/VOx multilayer is deposited by using a radio frequency (RF) sputtering system. The VOx/ZnO/VOx thin film shows the high temperature coefficient of resistance (TCR) of $-3.12%/^{\circ}C$ and the low sheet resistance of about 80 $k{\Omega}/sq$ at room temperature. The responsivity and detectivity of the bolometer are measured as a function of modulation frequency.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1269-1272
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• 2005

Sandwich structures, which are composed of thick core between two thin faces, are commonly used in many engineering applications because they combine high stiffness and strength with low weight. In this research, we have investigated the embossing configuration at the sheet metal shape through research with regard to the construction that the hardness and stiffness are excellent, and formability is advantage as inner structure. Through the FLD analysis according to the pattern changes, we have confirmed the forming possibility and variation of the aspect thickness. Also, we have fabricated the embossing press mold according to the pattern changes, and obtained the embossing inner structure the forming experiments.

• Transactions of Materials Processing
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• v.16 no.2 s.92
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• pp.138-143
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• 2007

Superplastic forming/diffusion bonding (SPF/DB) processes were analyzed using a 3-D rigid visco-plastic finite element method. A constant-triangular element based on membrane approximation and an incremental theory of plasticity are employed for the formulation. The coulomb friction law is used for interface friction between tool and material. Pressure-time relationship for a given optimal strain rate is calculated by stress and pressure values at the previous iteration step. In order to improve the contact searching, hierarchical search algorithm has been applied and implemented into the code. Various geometries including sandwich panel and 3 sheet shape for 3-D SPF/DB model are analyzed using the developed program. The validity fer the analysis is verified by comparison between analysis and results in the literature.

• Transactions of Materials Processing
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• v.16 no.4 s.94
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• pp.239-243
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• 2007

Superplastic forming/diffusion bonding(SPF/DB) has been widely used in the automotive and aerospace industry since it has great advantages to produce very light and strong components. Finite element method(FEM) is used to model the SPF/DB process of 3-sheet sandwich panel to predict the pressure-time curve and to analyze the process parameters. In order to eliminate defects of the part, a new pressurization scheme is proposed. Contrary to the conventional one-step pressurization, which causes the folding at the DB joint, two-step pressurization can eliminate the folding. Effect of pressurization cycle was investigated by using FE analysis and proper pressurization cycle is proposed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.25-31
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• 2018

Recently, research on the development of lightweight vehicle bodies is increasing continuously as a response to fuel economy regulations. To reduce the weight of a vehicle body, a conventional steel plate has been substituted by light weight material with high specific strength and the jointing of multi-materials is generally applied. On the other hand, the customer's demand for safety and emotional quality in NVH (Noise, Vibration and Harshness) is becoming increasingly important. Therefore, a light weight with proper strength and NVH quality is needed. In the view of light weighting and NVH quality, the application of a vibration proof steel plate can be an effective solution but the formability of a sandwich panel is different with a conventional steel sheet. Therefore, careful analysis of formability is required. This study aims to characterize the formability of a sandwich high-strength steel plate. The high-strength steel plates of different thicknesses with resistance spot welding and sealer bonding were analyzed using forming limits diagram through a cup drawing test.

• Steel and Composite Structures
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• v.26 no.6
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• pp.771-791
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• 2018

This paper deals with the low velocity impact response and dynamic stresses of composite sandwich truncated conical shells (STCS) with compressible or incompressible core. Impacts are assumed to occur normally over the top face-sheet and the interaction between the impactor and the structure is simulated using a new equivalent three-degree-of-freedom (TDOF) spring-mass-damper (SMD) model. The displacement fields of core and face sheets are considered by higher order and first order shear deformation theory (FSDT), respectively. Considering continuity boundary conditions between the layers, the motion equations are derived based on Hamilton's principal incorporating the curvature, in-plane stress of the core and the structural damping effects based on Kelvin-Voigt model. In order to obtain the contact force, the displacement histories and the dynamic stresses, the differential quadrature method (DQM) is used. The effects of different parameters such as number of the layers of the face sheets, boundary conditions, semi vertex angle of the cone, impact velocity of impactor, trapezoidal shape and in-plane stresses of the core are examined on the low velocity impact response of STCS. Comparison of the present results with those reported by other researchers, confirms the accuracy of the present method. Numerical results show that increasing the impact velocity of the impactor yields to increases in the maximum contact force and deflection, while the contact duration is decreased. In addition, the normal stresses induced in top layer are higher than bottom layer since the top layer is subjected to impact load. Furthermore, with considering structural damping, the contact force and dynamic deflection decrees.

• Composites Research
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• v.29 no.4
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• pp.203-208
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• 2016

Metal and polymer sandwich composites, which are made of sheet metal sheath and polymer or fiber reinforced plastic core, have been reconsidered as an alternative to sheet metal due to their lightness and multifunctional properties such as damping and sound-proof properties. For the successful applications of these composites, the delamination prediction based on the adhesion strength is important element. In this study, the numerical simulation of the delamination behavior of polymeric adhesive tapes with metallic surfaces was performed using cohesive zone elements and finite element software. The traction-separation law of the cohesive zone element was defined using the fracture energy derived from peel mechanics and experimental results from peel test and implemented in finite element software. The peel test of the polymeric adhesive film against steel surface was simulated and compared with experiments, demonstrating reasonable agreement between simulation and experiment.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.332-335
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• 2007

The superplastic forming (SPF) has been widely used in the automotive and aerospace industry because it has great advantages to produce very light and strong components. Finite element method (FEM) is used to model the process of superplastic forming/diffusion bonding (SPF/DB), to predict the pressure-time curve and to analyze the process parameter. In this study, process design of SPF/DB is carried out a 3-sheet sandwich part. SPF/DB process with pressure control was analyzed by using finite element method. For obtaining proper shape, step-by-step pressurization is proposed. The first step of SPD/DB process is obtained by applying of pressure in patches. From the next step it applied pressure to all regions (between inner sheets, between inner and face sheets). By using the proposed pressurization scheme, deficit in part shape is found to be eliminated.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.5 s.170
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• pp.152-158
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• 2005

Inner structured and bonded panel, or ISB Panel, as a kind of sandwich type panel, has metallic inner structures which have low relative density, due to their dimensional shape of metal between a pair of metal skin sheets or face sheets. Previous works showed that ISB panels containing inner structures formed as repeated pyramidal shapes saved weight up to $60\%$ in condition of same stiffness comparing with solid sheet. In this work, woven metal is adapted to inner structures replacing pyramidal structures. The test specimens of ISB panel containing woven metal made by multi-point electric resistance welding and 3-point bending test have been carried out. The results of experiments and comparisons of process parameters, stiffness and failure mode are discussed.