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A report of 39 unrecorded bacterial species in Korea belonging to the classes Betaproteobacteria and Gammaproteobacteria isolated in 2018

  • Kim, Yong-Seok;Yi, Hana;Kim, Myung Kyum;Seong, Chi-Nam;Kim, Wonyong;Jeon, Che Ok;Kim, Seung-Bum;Im, Wan-Taek;Joh, Kiseong;Cha, Chang-Jun
    • Journal of Species Research
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    • v.9 no.4
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    • pp.346-361
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    • 2020
  • In the project of a comprehensive investigation of indigenous prokaryotic species in Korea, a total of 39 bacterial strains phylogenetically belonging to the classes Betaproteobacteria and Gammaproteobacteria were isolated from various environmental sources such as soil, cultivated soil, sludge, seawater, marine sediment, algae, human, tree, moss, tidal flat, beach sand and lagoon. Phylogenetic analysis based on 16S rRNA gene sequences revealed that 39 strains showed the high sequence similarities (≥98.7%) to the closest type strains and formed robust phylogenetic clades with closely related species in the classes Betaproteobacteria and Gammaproteobacteria. In the present study, we report 14 species of 9 genera of four families of two orders in the class Betaproteobacteria and 25 species of 21 genera of 15 families of eight orders in the class Gammaproteobacteria, which have not been reported in Korea. Morphological, biochemical, and physiological characteristics, isolation sources, and NIBR deposit numbers are described in the species descriptions.

A Study on Designs for the Pulley and Electric-wire for 'HELLO' Artwork at Incheon Airport Terminal 2 (인천공항 제2여객터미널 'HELLO' 작품의 풀리와 전선의 디자인에 관한 연구)

  • Kang, Hee-Ra
    • Journal of Digital Convergence
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    • v.16 no.10
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    • pp.415-420
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    • 2018
  • This study focused on the designs for the pulley and electric cables for Hello, the artwork displayed at the Great Hall at the Incheon International AirportTerminal 2. This study presented designs for the pulley and cables, and the parts were applied and tested to analyze their durability and problems. Improvements were made for the final production of the work and three samples were produced respectively for the designs of the pulley and the cables. As are sult, this study found that for the pulley, the most efficient design was to fix the cables at its center. In the case of cables, it was most efficient touse flat cables with a wire in the core. These designs were applied to the artwork Hello, which is currently installed at the Great Hall at the Incheon International Airport Terminal 2. Future research will be conducted to make further improvements to the cable sand the pulley.

The Optimum Binder Ratio for High-Strength Self-Leveling Material (고강도 Self-Leveling재의 최적 결합재비)

  • Kim, Jin-Man
    • Journal of the Korea Institute of Building Construction
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    • v.2 no.4
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    • pp.89-98
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    • 2002
  • Self-leveling material(SLM) is one of the floor finishing materials which make flat surface like as water level by itself in a short time. So it is possible to increase construction speed and enhance economical efficiency In this study, author intended to develop SLM for the industrial warehouse and factory loading heavy weight machinery and vehicles. The demanded properties for this type of SLM are above 200mm of flow value and above 300kgf/$cm^2$ of 28-days compressive strength. To possess demended strength and fluidity, SLM have to be composed of many types of binders and chemical additives. So it is difficult to decide suitable mixing proportion of composition materials. In this study, author investigated the weight percentage effect of main composition materials for high-strength self-leveling material, by experimental design such as tables of orthogonal arrays and simplex design, and by statistical analysis such as analysis of variance and analysis of response surface. Variables of experiments were ordinary Portland cement(OPC), alumina cement(AC), anhydrous gypsum(AG), lime stone(LS) and sand, and properties of tests were fluidity of fresh state and strength of hardened state. Results of this study are showed that suitable mix proportions of binders for the high strength self-leveling materials are two groups. One is 78~85.5% OPC, 7.5~9.5% AC, 9~12.5% AG and the other is 72.5~78% OPC, 9~12.5% AC, 13~15% AG.

An Analysis of Ecological Habitat Characteristics in the Nonsan Stream and Yanghwa Stream (논산천과 양화천 수계 내 하천 생물서식처의 특성 분석)

  • Ahn, Tae-Woong;Ahn, Hong-Kyu;Chun, Seung-Hoon;Choi, Jun-Kil;Ha, Sung-Ryong;Oh, Jong-Min
    • Journal of Environmental Impact Assessment
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    • v.19 no.2
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    • pp.127-140
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    • 2010
  • This study investigates the relation between the location of a habitat and the ecological connections according to the habitat type in the riparian zone at the Nonsan Stream and Yanghwa Stream. Stream habitat is classified into nine types for the aquatic insects and fish. For vegetation and birds, habitat is classified into two types of medium-scale streams, including both physical and chemical streams are analyzed accordingly. Nowadays, The fundamental goal of the river environment restoration enterprise is the rehabilitation or the restoration of the characteristic the river scenic or environment. For instance, The Channel habitats which has physical nature environment such as flat, speedy rapids, or closed-channel wetland, pool are constructed by artificial, Consequently, make them autogenesis smoothy eco-environment. However, the river environment are controlled not only physical environment, but it also need reasonable the quality of the water to compose smoothly. Finally, understanding what influence are effecting on physical habitat environment which are made by natural factors to water quality are very important factor for the river environment restoration enterprise Therefore, In this research, we are targeting to a basin to investigate the environment of the physical channel habitat and evaluate the changing of the water quality. This results will be a important characteristic that can judge the physical habitat and reciprocality connected to the water quality or adequacy of restoration technology. Therefore in this study, as a step to quantify functions and values of habitats and definite factors to perform habitat, we selected a representative stream of sand-stream, gravel-stream to classify habitat characteristics and quantified the physical, chemical, biological characteristics.

The Study for Performance TestㆍVerification Standard, Form approval procedure(draft) of OSBA (생물정화제제의 성능시험ㆍ검정기준, 형식승인절차(안) 등에 관한 연구)

  • Chung Jin-Won;Yoon Joo-yong;Shin Jae-Rouk;Kim Han-Gyu
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.6 no.2
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    • pp.16-27
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    • 2003
  • For the last decade, some 400 small and large oil spill accidents have occurred every year. Such accident blow a serious damage to the marine resource and ecosystem, which can't be estimated in terms of economic and environmental losses. The physical/chemical methods used currently may be effective at the initial stage of accidents, but they can't serve to remove the spilled oil completely. Moreover, the dispersant may lead to a secondary contamination detrimental to the lives inhabiting wet lands, beaches and tidal zone. Thus, a new decomposing technology Is required for the environmentally sensitive areas. Bioremediation is the active use of biological techniques to mitigate the consequences of a spill using biological processes and refers both of stimulation of pollutant biodegradation and/or to enhance ecosystem recovery Bioremediation is an economically attractive method for the clean-up of oil-contaminated area. Bioremediation has been demonstrated to be an effective oil spill countermeasure for use in cobble, sand beach, salt marsh, and mud flat environment.

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A STUDY ON THE SHEAR BOND STRENGTH OF THE PORCELAIN LAMINATE ACCORDING TO SURFACE ROUGHNESS OF THE CUT ENAMEL (삭제된 법랑질의 표면거칠기에 따른 도재 라미네이트의 전단결합강도에 관한 연구)

  • Park, Bong-Seok;Cho, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.19 no.1
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    • pp.159-168
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    • 1994
  • The purpose of this study was to evaluate the shear bond strength of the porcelain laminate specimens according to the surface roughness of the cut enamel of human anterior teeth. Flat enamel surfaces were prepared in 30 extracted human anterior teeth with diamond disc which were divided into two groups. Group 1 Coarse enamel surface group prepared with LVS-3 bur. Group 2 Fine enamel surface group prepared with superfine diamond bur. 30 teeth specimens of two group were stored in normal saline during 24 hours. 30 disk - type porcelain laminate specimens with diameter 4mm and thickness 1mm were made and sand - blasted on internal surface which were to cemented on enamel surface. Porcelain laminate specimens were cemented on enamel surface with Choice Veneer System (Bisco Dental, U.S.A) according to manufacture's instructions. All teeth specimens of two groups were manipulated with same method and stored In normal saline before testing. An Universal Testing machine (Model No.UTM-4206,Instron, U.S.A) was used to apply shear loads in the vertical directed, and the force required for separation was recorded with a cross head speed of 3mm/min and 500kg in full scale. The results were as follow ; 1. The mean shear bond strength of coarse surface group was 36.30kg and that of fine surface group was 44.39 kg, but there was no significant difference in breaking strength of two groups(p>0.05).

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A report of 17 unrecorded bacterial species of Korea belonging to the phylum Bacteroidetes

  • Cho, Jang-Cheon;Seong, Chi Nam;Joh, Kiseong;Cha, Chang-Jun;Bae, Jin-Woo;Yi, Hana;Lee, Soon Dong;Kim, Myung Kyum;Yoon, Jung-Hoon
    • Journal of Species Research
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    • v.7 no.3
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    • pp.231-239
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    • 2018
  • While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

A Study on the Behavior of PHC-W Retaining Wall Method Based on the Numerical Analysis Results (수치해석 결과를 이용한 PHC-W흙막이공법의 거동에 관한 연구)

  • Choi, Jeong Pyo;Jin, Hong Min;Kim, Chea Min;Kim, Sung Su;Choi, Yongkyu
    • Journal of the Korean Geotechnical Society
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    • v.33 no.2
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    • pp.5-15
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    • 2017
  • PHC-W retaining wall method is one of the economical retaining wall methods. PHC-W pile used in PHC-W retaining wall method has special shape with flat surfaces so that the PHW-C retaining wall, with overlapped piles, shows outstanding vertical control and impermeability. In order to evaluate two types of retaining walls, numerical analysis were performed. The selection of cases depended on N values of the ground and ground properties, and two types of PHC-W retaining walls (defined as type A and B) were constructed. For a case that consists of inorganic clay and sand with less than 30 of N value, the maximum excavation depths for type A and B were respectively 10.5 m and 11.0 m. At the other case of which N value is above 30, the depths were 17.0 m and 19.5 m. From the results, it was found that maximum excavation depth, horizontal displacement, and safety factor for flexural strength of the wall were influenced by ground properties.

Development of Algorithms for Correcting and Mapping High-Resolution Side Scan Sonar Imagery (고해상도 사이드 스캔 소나 영상의 보정 및 매핑 알고리즘의 개발)

  • 이동진;박요섭;김학일
    • Korean Journal of Remote Sensing
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    • v.17 no.1
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    • pp.45-56
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    • 2001
  • To acquire seabed information, the mosaic images of the seabed were generated using Side Scan Sonar. Short time energy function which is needed for slant range correction is proposed to get the height of Tow-Fish to the reflected acoustic amplitudes of each ping, and that leads to a mosaic image without water column. While generating mosaic image, maximum value, last value and average value are used for the measure of a pixel in the mosaic image and 3-D information was kept by using acoustic amplitudes which were heading for specific direction. As a generating method of mosaic image, low resolution mosaic image which is over 1m/pixel resolution was generated for whole survey area first, and then high resolution mosaic image which is generated under 0.1m/pixel resolution was generated for the selected area. Rocks, ripple mark, sand wave, tidal flat and artificial fish reef are found in the mosaic image.

A report of 24 unrecorded bacterial species in Korea belonging to the Phyla Proteobacteria and Bacteroidetes isolated in 2020

  • Kim, Ju-Young;Yoon, Jung-Hoon;Joh, Kiseong;Seong, Chi-Nam;Kim, Won-Yong;Im, Wan-Taek;Cha, Chang-Jun;Kim, Seung-Bum;Jeon, Che-Ok;Seo, Taegun;Kim, Myung Kyum
    • Journal of Species Research
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    • v.11 no.3
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    • pp.133-142
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    • 2022
  • In 2020, 24 bacterial strains were isolated from algae, kudzu leaf, mud, pine cone, seashore sand, sea water, soil, tidal flat, and wetland from the Republic of Korea. Isolated bacterial strains were identified based on 16S rRNA gene sequences, and those exhibiting at least 98.7% sequence similarity with known bacterial species, but not reported in Korea, were highlighted as unrecorded species. These isolates were allocated to the phyla Bacteroidetes and Proteobacteria as unrecorded species in Korea. The four Bacteroidetes strains were classified into the families Chitinophagaceae, Flavobacteriaceae, and Sphingobacteriaceae (of the orders Chitinophagales, Flavobacteriales, and Sphingobacteriales, respectively). The 20 Proteobacteria strains belonged to the Aeromonadaceae, Marinobacter, Microbulbiferaceae, Enterobacteriaceae, Erwiniaceae, Morganellaceae, Yersiniaceae, Lysobacteraceae, Halomonadaceae, Moraxellaceae, Pseudomonadaceae, Steroidobacteraceae, Xanthomonadaceae, and Myxococcaceae (of the orders Aeromonadales, Alteromonadales, Cellvibrionales, Enterobacterales, Lysobacterales, Oceanospirillales, Pseudomonadales, Steroidobacter, Xanthomonadales, and Myxococcales). This study focused on the description of 24 unreported bacterial species in Korea in the phyla Bacteroidetes and Proteobacteria belonging to six classes.