• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1339-1347
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• 2010

In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbon (TPH) (9,000-4,5000 mg/g-soil) were Cynodon dactylon, Persicaria lapathifolia, and Calystegia soldanella (a halophytic species). Among the 24 plant species, the following have been known to be effective for oil removal: C. dactylon, Digitaria sanguinalis, and Cyperus orthostachyus. Denaturing gradient gel electrophoresis (DGGE) profile analysis showed that the following pairs of plant species had highly similar (above 70%) rhizobacterial community structures: Artemisia princeps and Hemistepta lyrata; C. dactylon and P. lapathifolia; Carex kobomugi and Cardamine flexuosa; and Equisetum arvense and D. sanguinalis. The major groups of rhizobacteria were Beta-proteobacteria, Gamma-proteobacteria, Chloroflexi, Actinobacteria, and unknown. Based on DGGE analysis, P. lapathifolia, found for the first time in this study growing in the presence of high TPH, may be a good species for phytoremediation of oil-contaminated soils and in particular, C. soldanella may be useful for soils with high TPH and salt concentrations. Overall, this study suggests that the plant roots, regardless of plant species, may have a similar influence on the bacterial community structure in oil-contaminated soil.

• BMB Reports
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• 제29권5호
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• pp.436-441
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• 1996

The palmitoyl acyl carrier protein (ACP) specific thioesterase (EC 3.1.2.14) from Iris pseudoacorus was purified and characterized. The thioesterase which was very unstable in relatively high salt concentrations was eluted using a co-gradient of Triton X-100 and low concentration of KCl or Na-phosphate from Q-Sepharose, DEAE-Sepharose, and hydroxyapatite chromatography. SDS-PAGE analysis showed a single band with a molecular weight of 35,000. The native molecular weight of approximately 37,000 was estimated by Sephacryl S-200 chromatography, indicating that the enzyme is a monomer. The thioesterase activity was inhibited about 75% and 50% by N-ethylmaleimide (2 mM) and phenylmethylsulfonyl fluoride (2 mM). respectively. The N-ethylmaleimide-inactivation was protected by sodium palmitate but the inactivation with phenylmethylsulfonyl fluoride was not protected. Oxidation of thiols by 2 mM 5.5'-dithio-bis-(2-nitrobenzoic acid) resulted in 65% inactivation of the enzyme. These results suggest that a cysteinyl residue is essential to the catalytic reaction of the enzyme. The enzyme activity was increased by sodium citrate and also by $Cu^{2+}$

• 대한기계학회논문집
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• 제19권7호
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• pp.1731-1740
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• 1995

Experimental investigations have been made to study the double-diffusive nature of convection of an initially stratified salt-water solution due to heating from below in a rotating cylindrical cavity. The objective is to examine the flow phenomena and the heat transfer characteristics according to the changes in temperature gradient, concentration gradient and rotating velocity of cavity. Thermal and solutal boundary conditions at side wall are adiabatic and impermeable, respectively. The top and bottom plate are maintained each at constant temperature and concentration. The cavity is put into a state of solid body rotation. Like the stationary case, the types of initially-formed flow pattern are classified into three regimes depending on the effective Rayleigh number and Taylor number; stagnant flow regime, single mixed-layer flow regime and successively formed multi-mixed layer flow regime. At the same effective Rayleigh number, the number of initially-formed mixed layer and its growth rate decrease as the effect of rotation increases. The temperature and concentration profiles are both uniform in each layer due to convective mixing in the layered-flow regime, but look both liner in stagnant flow regime and single mixed-layer flow regime. At the interface between adjacent layers, the temperature changes smoothly but the concentration changes rapidly.

• 미생물학회지
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• 제14권4호
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• pp.176-185
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• 1976

An improved procedure for the rapid purification of glucose-6-phosphate dehydrogenase from extracts of Saccharomyces cerevisiae was developed by using affinity chromatography. Among six affinty media tested, NADP$^{+}$-agarose and Affi-gel Blue were more effective than others (i.e., Affi-gel Red, AMP-agarose, ATP-agarose, and NAD$^{+}$-agarose). Conditions to desorb the enzyme bound to the affinity media were examined to increase the purity as well as yield. The best result was obtained when the column was developed with a linear gradient of KCl (0-1.0M). In case of Affi-gel Blue, introduction of NAD$^{+}$ (15mM) washing step prior to the salt gradient was most effective to remove NAD$^{+}$-binding proteins. For a large scale preparation of G-6-P dehydrogenase higher recovery was obtained by Affi-gel Blue than NADP$^{+}$-agarose, however, the purity of the enzyme was decreased by 10 times if the former was used as the affinity medium. The capacity of Affi-gel Blue for G-6-P dehydrogenase was found to be 5 times higher than that of NADP$^{+}$-agarose. Furthermore Affi-gel Blue could be reused repeatedly and its preparation is relatively easier and less expensive than NADP$^{+}$-agarose.X> +/-agarose.

• 대한기계학회논문집
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• 제18권12호
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• pp.3297-3304
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• 1994

Experimental investigation have been made to study the double-diffusive nature of convection of an initially stratified salt-water solution heated from below in a cylindrical cavity. The objective is to examine the process of mixed-layer formation, the flow phenomena, the heat transfer characteristics, and temperature and concentration distribution according to the changes in the effective Rayleigh number based on the reference height which represents the relation of temperature and concentration gradient. The types of initially formed flow pattern are categorized in three regimes depending on the effective Rayleigh number ; stagnant flow regime, single mixed-layer flow regime and successively formed multiple mixed-layer flow regime. The temperature and concentration profiles are both uniform in each layer due to convective mixing in the layered flow regime, but both linear in stagnant flow regime and single mixed-layer flow regime. At the interface between adjacent layers, the temperature changes smoothly but the concentration changes rapidly. The layers expand by diffusion of concentration through the interface along with its random fluctuation.

• Bulletin of the Korean Chemical Society
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• 제24권10호
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• pp.1433-1436
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• 2003

A new method for the simultaneous determination of heavy metal ions in cigarette material by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed. The cigarette material was digested by microwave digestion. Lead, cadmium, mercury, nickel and tin ions in the digested samples were pre-column derivatized with tetra-(2-chlorophenyl)-porphyrin ($T_2$-CPP) to form color chelates, which were then enriched by solid phase extraction with a $C_{18}$ cartridge. The chelates were separated on a Waters Xterra$^{TM}RP_{18}$ column by gradient elution with methanol (containing 0.05 mol/L pyrrolidine-aceticacid buffer salt, pH = 10.0) and acetone (containin0.05 mol/L pyrrolidine-acetic acid buffer salt, pH = 10.0)as mobile phase at a flow rate of 0.5mL/min and analyzed with a photodiode array detector from 350-600 nm. The detection limits of lead, cadmium, mercury, nickel and tin were 4,3,3,8 and 5 ng/L, respectively, in the original samples. This method was afforded good results.

• 대한화학회지
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• 제34권1호
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• pp.69-75
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• 1990

모자나이트 광물 중에 포함된 각각 희토류 원소를 이온회합 크로마토그래피 방법으로 분리 및 정량하였다. 정량하기 전에 양이온교환수지(Dowex 5OW-X8) 칼럼으로 희토류 원소를 집단 분리하였다. 이 때 수지 칼럼에 의하여 희토류 원소가 정량적으로 회수되며, 시료 중의 공존이온으로부터 깨끗이 분리됨을 방사성 추적자 및 유도쌍 결합 분광-질량분석법(ICP-MS)으로 각각 확인 하였다. 역상 칼럼($\mu$-Bondapak $C_{18}$)에 의하여 미량(ppm)의 희토류 원소를 pH 4.6의 $\alpha$-hydroxyisobutyric acid으로 0.05M부터 0.3M까지 기울기 용출하여 각각 분리하였다. 희토류 원소와 PAR (4-(2-pyridylazo)-resorcinol monosodium salt)과의 포스터 칼럼 착색반응을 통하여 각각의 희토류 원소를 검출하였다.

• 한국토양비료학회지
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• 제41권2호
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• pp.83-93
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• 2008

간척지에서 식생의 분포에 대한 평가는 토양관리 및 환경적 연구를 위해 필요하다. 본 연구는 한국 중부 서해안 경기만 내의 3개 간척지에서 식생의 분포와 토양 염농도 간의 관계를 규명하기 위하여 실시하였다. 식생이 연속분포를 보이는 지점에서 토양 염농도를 측정하고 출현식물종을 분류하여 연속분포의 특성을 기술하였다. 간척지 내에 나타나는 식생의 공간변이는 부분적으로 이루어지지만 각각의 공간변이를 토양 염농도를 기준으로 연결한 결과 토양 염농도 구배와 내염성 식물종의 분포가 일치하는 연속분포(continuum distribution)를 보여, 토양 염농도가 높은 곳은 내염성 식물종이, 낮은 곳은 비내염성 식물종이 분포하였다. 연속분포는 간척 경과 년 수가 오래된 곳에서 유형이 다양하고 명확하게 구분되나, 간척 초기 지역에서는 연속분포 현상이 뚜렷하지 않았다. 연속분포 유형은 순차적 유형과 비순차적 유형으로 구분되었다. 순차적 유형은 토양 염농도가 높은 곳을 기점으로 할 경우 토양 염농도가 연속적으로 낮아지는 방향으로 이어지며 식물종도 이에 따라 선구종 염생식물(pioneer halophyte)에서 저염생식물(facultative halophyte), 중성식물(glycophyte)의 순으로 변화하였다. 비순차적 유형은 비연속적 토양 염농도의 변화에 의해 형성되며, 식생의 분포도 단계적 변화를 보이지 않고 비정형적이다. 간척 경과 년 수가 오래된 지역에서는 토양 염농도가 높은 식생 고사지역이 있고 식생 비분포지와 함께 이 지점을 기점으로 식생의 연속분포가 형성되었다.

• 지구물리와물리탐사
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• 제14권3호
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• pp.220-226
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• 2011

탄성파동방정식에서는 변위가 수직 및 수평방향으로 정의된다. 실제 탐사에서는 수직변위와 수평변위를 모두 측정할 수 있기 때문에 이를 이용하여 방향성을 갖는 변위벡터를 구성할 수 있다. 본 연구에서는 이러한 변위벡터의 크기를 목적함수로 이용하는 주파수 영역 탄성파 파형역산 기법을 제안하고자 한다. 변위벡터 목적함수는 주파수 영역 파형역산 알고리듬에 적용할 경우 기존의 역전파 알고리듬과 동일한 방식으로 역산을 수행할 수 있다. 변위벡터 목적함수를 이용하여 Marmousi 모델과 SEG/EAGE 암염 모델의 합성탄성파 자료를 역산한 결과, 기존의 역산기법에 비해 RMS 오차가 안정적으로 감소하였다. 특히, Marmousi 모델의 밀도와 SEG/EAGE 암염 모델의 암염 하부의 저속도층을 실제 모델에 더 가깝게 구현할 수 있었다. 변위벡터의 크기를 목적함수로 사용할 경우 경사방향이 수치적으로 불안정한 형태로 정의되므로 이를 안정화시키기 위한 추가적인 연구가 필요할 것이다. 또한 본 논문에서 제안한 변위벡터 목적함수를 이용한 파형역산을 수행하기 위해서는 다성분 탐사자료 획득이 필수적이므로 육상탐사에서의 다성분 탐사나 해저면 다성분탐사(OBC, Ocean Bottom Cable) 등의 연구와 병행되어야 할 것이다.

• ALGAE
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• 제35권4호
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• pp.361-373
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• 2020

The community dynamics of benthic diatoms in the hypersaline environment are investigated to advance our understanding how salinity impacts marine life. Diatoms were sampled in the two salterns encompassing salt Ponds, ditches, and seawater reservoirs (n = 11), along the salinity gradient (max = 324 psu), and nearby tidal flats (n = 2). The floral assemblages and distributions across sites and stations showed great variations, with a total of 169 identified taxa. First, not surprisingly, higher diversity of benthic diatoms was found at natural tidal flats than salterns. The saltern diatoms generally showed salinity dependent distributions with distinct spatial changes in species composition and dominant taxa. Biota-environment and principal component analysis confirmed that salinity, mud content, and total nitrogen were key factors influencing the overall benthic community structure. Some dominant species, e.g., Nitzschia scalpelliformis and Achnanthes sp. 1, showed salinity tolerance / preference. The number of diatom species at salinity of >100 psu reduced over half and no diatoms were found at maximum salinity of 324 psu. The highest salinity for the observed live diatoms was 205 psu, however, a simple regression indicated a theoretical salinity threshold of ~300 psu on the survival. Finally, the indicator species were identified along the salinity gradient in salterns as well as natural tidal flats. Overall, high species numbers, varying taxa, and euryhaline distributions of saltern diatoms collectively reflected a dynamic saltern ecosystem. The present study would provide backgrounds for biodiversity monitoring of ecologically important microalgal producers in some unique hypersaline environment, and elsewhere.