• 한국어병학회지
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• 제5권1호
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• pp.1-7
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• 1992

CHSE-214 무지개송어 세포주는 10% fetal bovine serum과 2mM-glutamine이 첨가된 Eagle's 기본배지에서 성장하였다. CHSE-214 세포주의 최적온도는 $20^{\circ}C$였다. IPN바이러스는 CHSE-214 세포주에서 복제되었으며, 세포변성효과를 나타내었다. 또한 IPN 바이러스의 감염시간에 따른 감염증상을 역위상차 현미경으로 관찰하였다. 감염 6-12시간 후 세포는 정상세포와 비슷하였다. 감염 18-24시간 후 세포는 일부가 정상 세포보다 더 둥근형태로 되었고, 일부세포는 세포배양용 플라스크 바닥에서 떨어져 부유상태로 되었다. 감염 30시간 후 세포는 더더욱 비정상적인 형태로 되었다. 감염 48~68시간 후 감염된 세포는 파열되었고, 아주 높은 세포 병변 효과를 나타내었다.

• 한국수산과학회지
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• 제43권4호
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• pp.325-330
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• 2010

The apparent digestibility coefficients of dry matter, crude protein and energy in white fishmeal, herring meal, anchovy meal, salmon meal, sardine meal, mackerel meal, squid meal, soybean meal, corn gluten meal and wheat flour were determined for olive flounder. Digestibility coefficients were determined using a reference diet and test diets that contained 70% of the reference diet mixture and 30% test ingredients. All diets contained 0.5% chromic oxide as a digestibility indicator. The fish averaging 220 g were held in 500 L tanks at a density of 20 fish per tank. Feces were collected from three replicated groups of fish using a fecal collection column attached to a fish-rearing tank. The apparent dry matter, crude protein and energy digestibility coefficient values observed were in the ranges 29-79%, 59-95% and 45-91%, respectively, for various test ingredients. The apparent dry matter, crude protein and energy digestibility of white fishmeal, herring meal, anchovy meal, salmon meal, sardine meal, mackerel meal and squid meal were significantly higher than those of soybean meal, corn gluten meal and wheat flour.

• 대한두개안면성형외과학회지
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• 제19권1호
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• pp.20-34
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• 2018

Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta). The present study was designed to evaluate the wound healing effects of O. keta-derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex). Methods: Full-thickness skin defects were made on the back of mice (n=60). The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group). We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment. Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups. Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic. Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.

• 한국수산과학회지
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• 제44권3호
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• pp.225-231
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• 2011

Prolactin (PRL) plays an important role in freshwater (FW) osmoregulation by preventing the loss of ions and the uptake of water in fish. Growth hormone (GH) promotes acclimation to seawater (SW) in several teleosts. We acclimated rainbow trout Oncorhynchus mykiss weighting $68.2{\pm}16.6$, $138.3{\pm}24$, and $287.5{\pm}42.1$ g in separate experiments to SW under slow-acclimation (SSW) or acute-acclimation (ASW) conditions, and then examined the PRL and GH mRNA levels using the real-time quantitative polymerase chain reaction. The PRL mRNA levels in all three experimental groups decreased significantly with both the SSW and ASW treatments, as compared to a control group kept in FW for 30 days. The GH mRNA levels increased with ASW in the largest fish, whereas the levels in the other groups did not change significantly. The mortality rate of the largest fish was lower than for the other groups, whereas the growth rate among the three experimental groups did not differ significantly. The growth rate of the ASW group was highest for the smallest fish. These results suggest that SW acclimation is associated with the gene expression levels of PRL and GH in relatively large rainbow trout. In addition, the fish mortality and growth rate on FW-SW transfer seem to be related to body weight, and the SW acclimation method may be applied to the hatcheries industry.

• 한국수산과학회지
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• 제47권2호
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• pp.103-113
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• 2014

This study compared the food quality of freshwater rainbow trout Oncorhynchus mykiss cultured (in Pyeongchang, Pyeongtaek, Jecheon and Geochang) with that of imported salmon Oncorhynchus keta. The proximate composition of the four types of rainbow trout was 74.4-75.7% moisture, 18.3-19.4% crude protein, 3.1-4.3% crude lipid, and 1.3-1.7% ash. There were slight differences in the proximate composition of the four types of rainbow trout. No significant (P>0.05) differences were found in the red color and odor and taste intensities of the four types of rainbow trout based on the Hunter a values, volatile basic nitrogen content, odor intensity using an electronic nose and taste intensity using an electronic tongue. The crude protein, total amino acid, and mineral contents of rainbow trout cultured in Pyeongtaek were superior or similar to those of the trout cultured in the other three places. The rainbow trout cultured in Geochang had the highest hardness, at 858.5 g.

• 한국의류학회지
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• 제30권4호
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• pp.567-581
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• 2006

The purpose of this research is to provide the basic data for the development of make-up color application system, based of Korean's skin tone and the preference in make-up color to enhance the effectiveness of the education of beauty in universities. The research was conducted by the previous studies, the analyses of sale's rate of hue-cosmetics, the analytic experiment of color of cosmetics by using Spectrum Color Analyzer and other experimental researches. This research, based on the results of three preliminary researches, shows the result of evaluation from perceivers which has been come out from the experiment of having one model in her twenties being changed with twenty-two different conditions of make-up. Here follows the result of the research. Firstly, there was difference on perceiving images in terms of the gender of perceivers and especially male-group tend to have clearly perceived the gap between elegance-greyish purple, orange-natural, red-classic on monochrome make-up and contrast make-up. Secondly, in terms of lip-colors, salmon pink and pink was regarded positively to both female and male subjects and to male subjects, greyish purple was thought to be better on darker skin-tone and to female subjects, better on lighter skin-tone. Thirdly, on image make-up, romantic gives intelligent image regardless of skin-tone and gender, especially gives more positive looks to male subjects. Natural and classic elements were perceived more positively on darker skin-tone and had bigger perceiving gap in female subjects. Fourthly, in preference rate, male subjects normally preferred the look with make-up than female subjects did and salmon pink and pink lip color was preferred on the darker skin-tone.

• 한국수산과학회지
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• 제27권1호
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• pp.83-88
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• 1994

연어, 산천어, 무지개송어 등 우리나라의 연어과 어류에 있어서 종의 식별 및 3배체 어류의 판정에 동위효소를 genetic marker로 활용할 수 있는지의 가능성을 타진하고자 LDH, MDH, IDH, a-GPDH, ME, 6-PGD, PGI 및 PGM 등 8개 동위효소에 대하여 골격근 조직을 중심으로 분석을 실시하였다. 이중 골격근 조직의 MDH-B와 IDH loci에서 종간에 뚜렷한 차이를 나타내었으며 특히 MDH-B loci의 b 유전자의 출현빈도는 연어나 산천어에서는 거의 나타나지 않았으나 무지개송어에서는 매우 높게 나타났다. 또한 IDH도 무지개송어와 산천어간의 genetic marker로 유용할 것으로 보인다. 한편, PGI는 3배체 어류 생산시 친어를 상호 대립유전자(allele)의 동형접합(homozygote)인 개체를 사용할 경우 이의 효율적인 판정을 위한 새로운 marker로 활용할 수 있는 가능성을 가지고 있는 것으로 나타났으며 다른 loci에서는 별다른 차이를 발견할 수가 없었다.

• 한국해양바이오학회지
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• 제1권2호
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• pp.119-125
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• 2006

어류의 insulin-like growth factor-I (IGF-I)의 생화학적 작용기작을 연구하기 위하여 한국산 조피볼락의 IGF-I cDNA 유전자 cloning을 행하였다. 완전한 cDNA 유전자 염기서열은 PCR과 RACE 방법을 통하여 얻어진 DNA로부터 결과를 얻을수 있었다. 결정된 IGF-I의 염기서열은 flounder, chinook salmon, human IGF-I의 염기서열과 비교한 결과 각각 93.6%, 90.7%, 85.4%의 높은 상동성을 보였다. 생화학적으로 활성이 있는 재조합 IGF-I을 얻기 위하여 IGF-I의 B-C-A-D domain 부분을 PCR로 얻은 뒤 E. coli BL21(DE3)에 넣어 overexpression 시켰다. Ni-NTA colummn을 사용하여 순수한 재조합 단백질을 정제할수 있었다. 정제된 단백질은 SDS-PAGE 상에서 7 kDa의 단일 band를 보여 주었으며 [$^3H$]-thymidine 결합정도를 측정하는 방법으로 활성을 가지고 있음을 확인할수 있었다.

• Fisheries and Aquatic Sciences
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• 제24권12호
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• pp.437-445
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• 2021

Roughscale sole (Clidoderma asperrimum) is only wild caught because basic reproductive research on this species is lacking and gamete production in an artificial setting has not been successful. Exogenous hormone treatment has been used to induce gonadal maturation and final spermiation in wild-caught individuals. In this study, the effects of an exogenous hormone on spermiation in roughscale sole was investigated by implanting different concentrations of a salmon gonadotrophin-releasing hormone analog (sGnRH; 0, 25, 50, and 100 ㎍/kg body weight) into male fishes. The control group did not produce sperm after 21 days post-implantation, and the duration of spermiation was shorter compared to the other groups. The spermiation period and milt amount differed among the hormone-treated groups according to the hormone concentration used. Milt volumes in the groups treated with 25 and 100 ㎍/kg sGnRH increased compared to the control group, whereas exogenous hormone treatment had no effect on the movable sperm ratio. The spermatocrit was high at the beginning of spermiation in all groups and then tended to decrease gradually over time except in the experimental group treated with 100 ㎍/kg sGnRH. Plasma levels of testosterone, 11-ketotestosterone, 17α, 20β-dihydroxy-4-pregnen-3-one were not significantly affected by the sGnRH treatments. Our results demonstrate that it is possible to prolong the spermiation period and increase milt volume by treating male roughscale soles with an exogenous hormone. In addition, the artificial hormone treatment did not affect sperm motility.

• Nuclear Medicine and Molecular Imaging
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• 제42권1호
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• pp.39-43
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• 2008

목적: 이 연구에서는 다발성 골수종에서 두개골과 중간 허벅지 이하 하지부를 포함한 전신 $^{18}F$-FDG PET/CT 영상 획득의 필요여부를 알아보았다. 대상 및 방법: 총 30명(평균나이: $59.4{\pm}11.7$세, 남/여 17/13명)의 환자를 대상으로 두개골과 중간 허벅지 이하 하지부를 포함한 전신 $^{18}F$-FDG PET/CT 영상을 총 45개 촬영하였다. PET 영상에서 두개골 또는 중간 허벅지 이하 하지부에 주변보다 비정상적인 국소 섭취증가를 보이는 병변 유무에 따라 두 그룹으로 나누어 나이, 성별, 병기 등의 임상소견 차이를 비교해보았다. 결과: 총 45개의 전신 PET/CT 영상에서 두개골 또는 중간 허벅지 이하 하지부 병변은 14명 환자(46.7%)의 22개 스캔(48.9%)에서 발견되었다. 그 중 두개골 병변은 중간 허벅지 이하 하지부 병변에 비해 유의하게 흔히 관찰되었다(86.4% 대 40.9%, p<0.005). 이 두 환자군 사이에 종양의 양을 반영하는 혈색소 수치, 혈청 칼슘 수치, 혈청 면역글로불린 수치, 뇨 면역글로불린 수치, 혈청 크레아티닌 수치에 유의한 차이는 없었다. 두개골 또는 중간 허벅지 이하 하지에 비정상적인 국소 FDG 섭취증가를 보인 22개의 PET 영상에서 두개골 또는 중간 허벅지 이하 하지 병변으로 인하여 Durie/Salmon plus 병기가 상승된 경우는 단 1개(4.5%, p>0.05)였다. 결론: FET 영상에서 두개골 또는 중간 허벅지 이하 하지 병변은 다발성 골수종에 있어 종양의 양 및 병기 평가에 유의한 영향을 주지 못했다. 따라서 다발성 골수종에서 머리와 다리를 포함한 전신영상을 꼭 촬영할 필요는 없으며, 머리를 포함한 몸통 영상획득 방법이 가장 적절할 것으로 보인다.