• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.31-31
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• 2022

Plant-derived antioxidants are used as a healthy diet and are known to inhibit various human diseases. In this study, we investigated free radical scavenging and antioxidant activity of extracts from three plants (Ammannia multiflora, Ammannia coccinea and Salix gracilistyla) with the most DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity from 196 plant extracts inhabiting along Nakdong River in Republic of Korea. The three extracts also have strong total antioxidant activity. Moreover, the extracts inhibited hydrogen peroxide (H₂O₂)-induced reactive oxygen species production and depolarized mitochondrial membrane potential in RAW264.7 macrophages. In zebrafish larvae, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescent intensity, induced by H₂O₂, was markedly reduced by the extracts of A. multiflora, A. coccinea and S. gracilistyla. Meanwhile, the extracts were upregulated Nrf2 and HO-1 expression, and an HO-1 inhibitor reversed the extract-induced oxidative responses both in vivo and in vitro. The data suggest that the extracts of A. multiflora, A. coccinea, and S. gracilistyla exert potential free radical scavenging and antioxidant capacities both in vivo and in vitro by activating the Nrf2/HO-1 signaling pathway.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.66-66
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• 2022

속성수로 활용되는 버드나무속 식물들은 생식기관과 영양기관의 성장 시기가 달라 형태적 특성 평가를 위해 수년간의 조사 기간이 요구된다. 따라서 바이오매스 우수 버드나무속 교잡종 육성의 성공 여부를 조기 판별하기 위한 식별 기술이 필요하다. DNA 마커는 식물의 생장단계와 관련 없이 탐색할 수 있으며 환경에 영향을 받지 않는 장점이 있다. 식물의 계통 분류 시 주로 사용되는 엽록체 DNA는 유전자 염기서열의 변이가 비교적 크지 않은 장점이 있으나 대부분의 활엽수에서 모계를 통해 유전되는 특징이 있다. 하지만 종간 교잡종의 식별은 각각의 부모종과 구분할 수 있어야 하므로 본 연구는 엽록체 DNA가 아닌 핵 DNA를 대상으로 분석하였다. 본 연구의 목적은 호랑버들을 암나무로 갯버들을 수나무로 인공교배하여 육성된 종간 교잡종을 식별하는 핵 DNA 마커를 탐색하는 것이다. 이를 위해 버드나무속에서 개발된 총 35개의 nSSR (nuclear Simple Sequence Repeat) 마커를 대상으로 호랑버들과 갯버들, 종간 교잡종의 식별 가능성을 평가하였다. 분석 결과 호랑버들과 갯버들, 종간 교잡종 간 차이를 나타내는 2개의 핵 DNA 마커를 선발하였다. 따라서 선발된 핵 DNA 마커를 활용하여 호랑버들과 갯버들, 종간 교잡종의 조기 식별에 활용이 가능할 것으로 사료된다.

• Korean Journal of Environment and Ecology
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• v.25 no.2
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• pp.189-210
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• 2011

As a part of improving the effect of stream restoration, the study selected 90 domestic natural streams that are not damaged and researched their riparian vegetations. Among them, the results of streams, whose naturalized ratio were more than 5 % and valley type flood plains were not developed, were excluded. By using the results of final 49 streams, plant communities and companion species that commonly appeared in high frequency, depending on stream type, were drawn. The result revealed that Quercus mongolica community, Hemiptelea davidii community and Salix koreensis community were representative in the mid-northern district (latitude: N $37{\sim}37.9^{\circ}$) with Salix koreensis community in the central district (latitude: N $36{\sim}36.9^{\circ}$) and Quercus serrata community in the southern district (latitude: $34{\sim}35.9^{\circ}$). For the stream type depending on altitude, Quercus serrata community and Phragmites japonica community were representative in the altitude of 200~500 m and there was no stream, in which flood plain is developed naturally, in the altitude of more than 500 m. In addition, the study drew representative communities depending on width of stretches of water at mean water level indicating the size of stream. Quercus serrata community was major vegetation at sites which are less than 20 m wide and Salix koreensis community was common at sites which are more than 20 m and less than 100 m wide of stretches of water at mean water level. Meanwhile the major vegetations at sites which are more than 100 m wide were Salix koreensis community, Morus bombycis community and Salix nipponica community. The study selected the kind of tree for planning riparian forest in flood plain by stream type on the basis of the result and suggested tree planting pattern that can be used in actual work by referring to the result of hydraulic review.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.3
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• pp.165-173
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• 2007

In the previous study, we reported the antioxidative and cellular protective effects of Jeju native plant extracts. In this study, we investigated the anti-oxidative, anti-wrinkle and whitening effects of new 37 plant extracts collected from self-growing plants in Jeju island. Their anti-oxidant activities were measured by the 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging assay and reactive oxygen species(ROS) scavenging assay in $Fe^{3+}-EDTA/H_2O_2$ system. The cytoprotective properties of 37 plant extracts were assessed in the rose-bengal sensitized photohemolysis of human erythrocytes. The inhibitory effect of 37 plant extracts on tyrosinase were investigated to assess their whitening efficacy. Finally, their anti-elastase activities were measured to predict the anti-wrinkle efficacy in the human skin. The results showed that the extracts of Myrica rubra stem bark and Securinega suffruticosa have the free radical scavenging activity($FSC_{50}:\;5,\;8{\mu}g/mL$, respectively), and the extracts of Quercus acutissima leaf and Securinega suffruticosa stem bark have the prominent ROS scavenging activity($OSC_{50}:\;0.009{\mu}g/mL$). Photohemolysis of erythrocytes in the presence of rose-bengal as a sensitizer was inhibited by the extracts of Securinega suffruticosa stem bark and Salix koreensis stem(${\tau}_{50}$, 895 min, 640 min at 50 ${\mu}g/mL$, respectively. Myrica rubra stem bark extract(77.8% at 200 ${\mu}g/mL$) and Salix koreensis stem extract(76.2% at 200 ${\mu}g/mL$) also have the inhibitory effect on tyrosinase and elastase activities, respectively. These results indicated that the stem park of Myrica rubra, Securinega suffruticosa, and Camellia japonica, the stem of Salix koreensis, and the leaf of Quercus aqutissima and Camellia japonica could have e benefitial effects when they are added as ingredients in cosmetics.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.124-131
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• 2010

This study was conducted to investigate the potentials for the forest restoration on reclaimed land by using willow trees (Salix koreensis Anderson) selected from a coastal reclaimed land made in inside of the Sihwa tide embankment. We first collected six individual willow trees that were the only tree species grown in the reclaimed land. Total 7 clones from cuttings of the collected trees and the control were grown in a greenhouse for two months prior to applying the different concentrations of NaCl solutions (0.0%, 0.1%, 0.5%, and 1.0%). One month after the NaCl application, the survival rates of clones from both the collected trees, and the control were significantly decreased in a NaCl dose-dependent manner. However, there was no significant difference between the collected trees and the control in terms of survival rate, hight and diameter of cuttings, and the numbers of leaves in greenhouse condition. In conclusion, the willow trees collected from the coastal reclaimed land showed no tolerance against NaCl compared to the control grown in ordinary soil, suggesting that further study is required to determine what the most important factor is to select salt tolerant tree species.

• Ecology and Resilient Infrastructure
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• v.4 no.3
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• pp.149-155
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• 2017

Willow plants are representative biomaterials used in river restoration and main target trees in stream managements. In order to understand the changes in the growth of Salix subfragilis and S. chaenomeloides with their stand ages, we investigated the density, height and basal area of stems and biomass at their different aged stands of the riparian zone of the sand-bed stream, the Nakdong River, Korea. We also developed allometric equations for estimating the biomass of these two species by establishing the relationship between diameter at breadth height and tree height with above-ground biomass. The stem density showed a sharp decrease for 3 years after germination for S. subfragilis and 6 years for S. chaenomeloides, resulting in strong self-thinning. The stem height of the two species increased to 7.5 m in 15 years for S. subfragilis, and to 14 m in 13 years for S. chaenomeloides. Aboveground biomass also increased rapidly at the early stage of growth. The biomass increased to 17 ton DM/ha in 13 years for S. subfragilis and to 1,110 ton DM / ha in 13 years for S. chaenomeloides. It is expected that the allometric equations of two Salix species derived from this study will be applied to the objectively estimating the biomass of willow plants for the management of floodplain trees in streams.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.3
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• pp.52-59
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• 2007

This study was carried out to select proper species for early stage replantation in cutting-slope area of the Baekdu Range roadside. In the cutting-slope area and upper forest area of the cutting-slope area, sample plots of 46 were selected and their vegetations and environmental factors were investigated. We found total 90 species in the 46 plots. We found Festuca arundinacea in 27 plots of the 46 plots. We also found Lespedeza bicolor in 26 plots, Eragrostis curvula in 22 plots, Artemisia princeps var. orientalis in 19 plots, Erigeron annuus and Pinus densiflora in 15 plots, Lactuca indica var. laciniata in 14 plots, Miscanthus sinensis in 13 plots, Oenothera odorata, Commelina communis, and Humulus japonicus in 11 plots, Lespedeza cuneata in 10 plots, Salix koreensis and Salix hulteni in 9 plots, Festuca rubra, Youngia denticulata, Aster scaber, and Festuca arundinacea in 8 plots, Chenopodium album var. centrorubrum in 7 plots, Patrinia villosa and Erigeron canadensis in 6 plots, and Setaria viridis, Digitaria sanguinalis, and Dactylis glomerata in 5 plots.The dominancy of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Miscanthus sinensis, Pinus densiflora, Festuca arundinacea, Festuca rubra, Artemisia princeps var. orientalis, Erigeron annuus, Lespedeza cuneata, Dactylis glomerata, Salix koreensis, Alnus hirsuta, Pueraria thunbergiana, Medicago sativa, Lactuca indica var. laciniata, and Digitaria sanguinalis were 13.41% 11.87%, 4.88%, 4.56%, 3.38%, 2.29%, 2.14%, 2.14%, 1.99%, 1.95%, 1.54%, 1.47%, 1.44%, 1.37%, 1.15%, 1.13%, 1.07% in the roadside cutting-slope, respectively. The dominancy of Rhododendron mucronulatum, Lindera obtusiloba, Rhus trichocarpa, Quercus mongolica, Lespedeza bicolor, Rhododendron schlippenbachii, and Quercus serrata 22.15%, 13.36%, 13.14%, 12.03%, 11.74%, 11.20%, and 11.14% in shrub layer of the upper forest area, respectively. The species of Rhododendron mucronulatum, Lindera obtusiloba, Rhus trichocarpa, Rhododendron schlippenbachii, Zanthoxylum schinifolium, Weigela subsessilis, Stephanandra incisa, Rhamnus davurica, Ligustrum obtusifolium, Lindera erythrocarpa, Rosa multiflora, Rubus coreanus, Symplocos chinensis for. pilosa, Smilax china, Prunus sargentii, and Staphylea bumalda may be more helpful in stabilizing of the cutting-slope area and making harmony with the surrounding forest area.

• Journal of Ecology and Environment
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• v.33 no.2
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• pp.157-164
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• 2010

We evaluated the restoration effect by introducing willows as a means of controlling invasions of giant ragweed (Ambrosia trifida L.) on a riparian site. Our preliminary survey demonstrated that a problematic exotic species, giant ragweed and the representative riparian species, Salix koreensis are in competitive exclusive relationship. We planted willows at 1 m intervals on the bank of the Dongmun stream at Munsan, Paju, in Central Western Korea as an experimental restoration practice. We installed two 50 m $\times$ 5 m sized restored and non-restored for this experimental study. The non-restored plots were located on river banks, which were covered with concrete blocks and left in itself without any treatment. The height of willow was measured after each of three consecutive growing seasons and compared with the height of the giant ragweed. Although the height of Salix gracilistyla did not achieve the height of the giant ragweed, the height of S. koreensis surpassed that of giant ragweed in the third year after introduction. The results were also reflected in the relative light intensity on the herb layer of willow stand, and thereby the relative light intensities of stands, which were dominated by S. koreensis or restored by introducing S. koreensis, 1.99 $\pm$ 0.33 (%, mean $\pm$ SD) and 1.92 $\pm$ 0.50 (%, mean $\pm$ SD), respectively were lower than those in the stands treated by S. gracilistyla, 3.01 $\pm$ 0.43 (%, mean $\pm$ SD). The giant ragweed stands receive full sunlight as there are no any vegetation layers higher than the herb layer formed by the giant ragweed. As the result of Detrended Correspondence analysis ordination based on naturally established vegetation, the stands dominated by willows and giant ragweed showed different species composition between both stands. The species composition of the restoratively treated sites resembled the reference sites more than the non-treated sites. The species diversity (H') of the sites restored by introducing S. koreensis and S. gracilistyla was higher than the non-restored site dominated by A. trifida. On the basis of our results, the restoration of riparian vegetation equipped with integrated features could contribute not only to the control of exotic plants including giant ragweed but also to ensure the diversity and stability of riverine ecosystems.

• Korean journal of applied entomology
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• v.53 no.2
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• pp.135-139
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• 2014

Anastatus orientalis Yang et Gibson(Hymenoptera : Eupelmidae) is the egg parasitoid of lantern-fly Lycorma delicatula. The natural parasitism showed that: (1)the highest parasitism rate of egg masses was 68.96% in Yantai Shandong; (2)the highest parasitism rate of eggs was 32.98% in Haidian Beijing; (3)the eggs of parasitoids hatched and emerged earliest in Yangling Shaanxi; emergence time of different populations in Yantai, Guangang Tianjin, Qinhuangdao Hebei and Haidian was similar; (4) the sex ratios were various among the populations ranging from 1.92 to 1.94; (5) parasitism rates of egg masses on Populus sp., Salix sp. and Toona sinensis were not significantly different, the highest parasitism rate of egg masses was 64.3% on T. sinensis, and the lowest rate was 27.4% on Ailanthus altissima; (6)parasitism rates of eggs on Populus sp., Salix sp., T. sinensis and A. altissima were not significantly different, about 30% averagely.

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.16-24
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• 2018

This study was investigated to select superior population and clones of Salix gracilistyla for promoting woody biomass resources through creating of short rotation coppices (SRC). Plant materials were collected from seven different population groups of S. gracilistyla and planted at two different nursery sites in Chuncheon and Yongin. Height and root collar diameter showed statistically significant differeces among population, nursery, and annual growth for three years (p<0.01). Aboveground biomass was harvested to investigate dry weight after 3 years from planting, the Youngwol population showed the highest yield as $4.8kg\;DW\;plant^{-1}$ in Chuncheon nursery which was more than double yield compared to the other nursery planted plants. However, Hongcheon and Wonju populations as $3.3kg\;plant^{-1}$, showed the best yield in Yongin nursery. In addition, there was a significant difference between collected populations and nurseries. But there was statistically significant different interaction between population (Pop) and nursery (Nur) (F value = 3.51, p<0.01). Therefore, the superior populations selected by this experiment could be cultivated as an excellent variety for woody biomass resources through the clonal test.