This study is being conducted in order to confirm the effects of inhaling method for aromatherapy on cortisol which is a stress hormone by the saliva test method. An attempt is being made to dertermine if there are any differences between the effect of lavender and rosemary when concidering their effects on the cortisolsl. The test shows a statistically significant decrease in the cortisol levesl(Table 4), and as far as the comparison of the effects between lavender(N=10) and rosemary(N=10) is concerned, the lavender group show a p-value of .005 which means that there are no statistical significances; while the rosemary group show p-values of .081 meaning that there is a decrease in cortisol levels, which is statistically significant(Table 5). It has been proven that the saliva test method is a practical and scientific method when confirming the effects of aromatherapy and also a convenient method for both of the test coordinator and the subjects. Results of all 20 subjects showed similar results obtained by means of conventional blood tests. However, the rosemary group shows statistically significant decrease in cortisol level compared to the lavender group, thus proving that the test method for studying the effect of aromatherapy on stress is valid. Further studies should be conducted in order to investigate the differences in the effects of the cortisol level at different concentrations of the aroma.
This review discusses the effects and roles of insect hormones and insect growth regulators (IGRs) on vitellogenesis in adult insects. Insect vitellogenesis is regulated by hormones such as juvenile hormone (JH), ecdysteroids, and neurosecretory hormones (ovaryecdysteroidogenic hormone : OEH) released by neurosecretory cells, diet, and other elements(male specific protein of sperm fluid). In the fat bodies, the vitellogenins are synthesized by the stimulation of JH released by corpus allatum (CA) and ecdysteroids produced by follicle cells with the ovary in most insects. Furthermore, vitellogenins are released into the hemolymph, transported to the ovarioles by carrier protein, and incorporated into oocytes for the developing ovary. Of IGRs, juvenile hormone and its mimics such as methoprene and pyriproxifen appear to have pharmacological effects such as membrane lysis, destruction of salivary grand and midgut epithlial cells, fat body cells, and ovarian tissue, and also anti-juvenile hormone such as precocenes I and II appear to have specific cytotoxicity such as inhibition of corpus allatum and oocytes development. These results suggest that IGRs may be useful as agents for integrated pest management.
The experimental female rats were ovariectomized and after 14day, they were injected intramuscullarly with estrogen of single dose, 2/100gm body weight.
After hormone injection, the animals were sacrificed by ether inhalation, the salivary glands were removed for microscopic examination and the histochemical preparations were observed. The results were as followed;
In the submaxillary gland of ovariectomized rats, the acinar cells have lesser PAS reactivity than control group, and-SH group reaction were slight.
In the estrogen injected-ovariectomized rats, aciner cells and striated duct cells have high reactivities for PAS, and-SS and SH group reaction were more increased than control group.
Objective: The objective of the study was to determine the relationship between plasma and salivary cortisol concentrations in beef cattle that were subjected to handling prior to sampling. Methods: Twenty-one Nguni cows of three age categories; 5 to 7 yr (n = 7), 8 to 10 yr (n = 6), and 11 to 13 yr (n = 8) were handled for five consecutive weeks. In the pen, a human avoidance test was performed and cattle responses to restraint in the chute and crush were observed. In addition, rectal temperature readings were taken and, faecal samples were collected and analysed for glucocorticoid metabolites. Through the handling and restraint process, excretory and vocalisation behaviour, as a sign of stress were observed and recorded. Thereafter, six cows were randomly selected and subjected to an adrenocorticotropic hormone (ACTH) challenge. Blood and saliva samples were extracted to determine cortisol concentrations. Results: Repeated handling affected (p<0.05) faecal glucocorticoid metabolites, rectal temperatures, avoidance distance, crush scores as well as urination and defaecation behaviour. Acclimation to handling was variable based on each respective parameter. Saliva cortisol concentrations increased and decreased significantly (p<0.001). A peak value of $136.78{\pm}15.869nmol/L$ was observed 30min after administration of ACTH, from a baseline value of $8.75{\pm}15.869nmol/L$. Plasma cortisol concentrations did not differ (p>0.05) across the time of sampling. A low and insignificant correlation (r = 0.0131, p>0.05) between plasma and saliva cortisol was therefore observed. Conclusion: We conclude that if beef cows are subjected to handling prior to sampling, a weak relationship exists between plasma and salivary cortisol levels.
Bulut, Gulay;Kosem, Mustafa;Bulut, Mehmet Deniz;Erten, Remzi;Bayram, Irfan
Asian Pacific Journal of Cancer Prevention
/
v.16
no.18
/
pp.8203-8210
/
2016
Adenocarcinomas (AC) are the most frequently encountered carcinomas. It may be quite challenging to detect the primary origin when those carcinomas metastasize and the first finding is a metastatic tumor. This study evaluated the role of sex hormone binding globulin (SHBG) positivity in tumor cells in the subclassification and detection of the original organ of adenocarcinomas. Between 1994 and 2008, 64 sections of normal tissue belonging to ten organs, and 116 cases diagnosed as adenoid cystic carcinoma and mucoepidermoid carcinoma of the salivary gland, lung adenocarcinoma, invasive ductal carcinoma of the breast, adenocarcinoma of stomach, colon, gallbladder, pancreas and prostate, endometrial adenocarcinoma and serous adenocarcinoma and mucinous adenocarcinoma of the ovary, were sent to the laboratory at the Department of Pathology at the Yuzuncu Yil University School of Medicine, where they were stained immunohistochemically, using antibodies against SHBG. The SHBG immunoreactivity in both the tumor cells and normal cells, together with the type, diffuseness and intensity of the staining were then evaluated. In the differential diagnosis of the adenocarcinomas of the organs, including the glandular structures, impressively valuable results are encountered in the tumor cells, whether the SHBG immunopositivity is evaluated alone or together with other IHC markers. Further extensive research with a larger number of cases, including instances of cholangiocarcinoma and cervix uteri AC [which we could not include in the study for technical reasons] should be performed, in order to appropriately evaluate the role of SHBG in the differential diagnosis of AC.
Journal of the Korea Academia-Industrial cooperation Society
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v.18
no.10
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pp.471-479
/
2017
The purpose of this study was to investigate changes in salivary cortisol content in 35 subjects before and after visiting Gotjawal Forest in Jeju. Cortisol raw samples were collected before and after visiting Gotjawal Forest and were analyzed by salivary cortisol enzyme immunoassay. The period of the study was from May 1, 2017 to June 30, 2017. There was no significant difference in cortisol content between the control and experimental groups before the visit, but the value of the experimental group significantly decreased afterwards. The mean value of cortisol in male subjects was lower than that of the control group. In the case of women, the value of the experimental group was lower than that of the control group. The post-visit cortisol content of the experimental group was significantly lower than that of the control group. In the post-visit analyses according to gender and group, there were significant differences between genders, groups, and combined effects of gender and group. This study provides basic data to prove the effects of forest bathing.
Journal of the Korean Institute of Landscape Architecture
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v.39
no.5
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pp.111-118
/
2011
With increasing interest in health promotion and quality of life, growing attention has been focused on the beneficial effects of urban green area. However, very few evidence-based approaches have been conducted on the health-related benefits of urban greenery. Therefore, this study examined the health-related benefits of green areas using physiological and psychological indices to obtain evidence-based data on these benefits. Twenty male university students were selected as subjects. Data were collected when participants viewed landscapes in a green area or an urban area for fifteen minutes. This research was reviewed and approved by the Ethics Committee of School of Medicine, Chungnam National University. Physiological data in the green area revealed significantly decreased heart rates, significantly increased high-frequency value of heart rate variability, an index of parasympathetic activity, and reduced salivary cortisol concentration, a stress hormone, compared to the urban area. Psychological tests showed the green area significantly reduced the negative mood state and psychological symptoms, and significantly increased the positive mood state. Our data provided evidence for the health-related benefits of green areas, and the findings of this study support that green areas can play a critical role in health promotion for urban residents, by positively affecting autonomic nervous and endocrinal activities.
Sungbo Cho;Santi Devi Upadhaya;Woo Jeong Seok;Seyoung Mun;Haeun Lee;Rudolf H. van der Veen;Kyudong Han;In Ho Kim
Journal of Animal Science and Technology
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v.65
no.6
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pp.1308-1322
/
2023
Minerals is required small amounts among various nutrients, but it has a significant impact on sow longevity and reproduction performance. This study was carried out to see the beneficial effects of marine-derived Ca-Mg complex on the reproductive performance of sows during four-parity periods. Seventy-two gilts ([Yorkshire × Landrace] × Duroc), with an average body weight of 181 kg, were randomly allocated to three groups; CON (basal diet), 0.3LC (CON - MgO - 0.3% limestone + 0.4% Ca-Mg complex), and 0.7LC (CON - MgO - 0.7% limestone + 0.4% Ca-Mg complex). During parity 3 and 4, the expression level of SCD gene was lower in the umbilical cord of piglets born to 0.3LC and 0.7LC sows compared with the CON sows. During parity 2, 3 and 4, SLC2A2 and FABP4 gene expressions were higher in the umbilical cord of piglets born to 0.7LC sows and the placenta of sows from 0.3LC groups, respectively. Ca-Mg complex increased (p < 0.05) Ca and Mg concentrations in sows and their piglets' serum as well as in colostrum regardless of parities. The serum vitamin D concentration was higher (p < 0.05) in their first parity, whereas serum prolactin and estrogen concentrations were higher (p < 0.05) during the fourth and third parity, respectively. The growth hormone concentrations were higher (p < 0.05) in the piglets born to sows during the first and second parity. The fat and immunoglobulin A (IgA) concentrations in colostrum were higher (p < 0.05) during the third and fourth parity, respectively. A reduction (p < 0.05) in salivary cortisol, epinephrine, and norepinephrine concentrations was observed in 0.3LC and 0.7LC sow groups compared with CON after farrowing regardless of parity, however before farrowing, a reduction in norepinephrine was observed. Before farrowing, the epinephrine and norepinephrine concentrations were higher (p < 0.05) during the first and second parity. After farrowing, the concentration of these hormones was higher during the second parity. Taken together, sows' parity and dietary Ca-Mg complex supplementation influenced serum metabolites, colostrum nutrients, stress hormones as well as the gene expressions related to lipid and glucose metabolism.
Background and objective: This study was conducted to find out the effects of healing farm resources and participant satisfaction on the level of stress and loyalty. Methods: The experiment was conducted on 18 subjects who participated in agro-healing activities (aged 49.5 years on average) and the concentration of cortisol, the level of stress and satisfaction with the services and program of a healing farm. The healing-agriculture activities were performed once a week, a total of 7 sessions, for 90 minutes and their saliva was collected before and after the activities. Results: The concentration of cortisol, a stress hormone, was statistically significantly reduced after the healing-agriculture activities in 5 out of 7 sessions. The total cortisol concentration showed statistically significant differences. The 6th session was comprised of different activities, compared to others, such as understanding weeds, managing a garden and making a pizza with fermentation enzymes, and the level of cortisol in the 6th session was the lowest after participating in the agro-healing activities, which indicated that the activities have the positive effect of lowering the level of stress. The level of stress was lowered from 18.39 to 16.78, which was consistent with the results of the concentration of cortisol. The level of satisfaction in the last session was below 50%, which indicated that the owner of the farm might be a little inexperienced in education and marketing on information and safety. The variables of participants including stress and satisfaction with services showed a strong influence on their loyalty. Conclusion: Satisfaction with services and loyalty to the healing farm showed a very high correlation, which was statistically significant. It also showed that participants' stress level and satisfaction with the services and program of the healing farm have a strong influence on their loyalty.
Jang, Hye Sook;Yoo, Eunha;Jeong, Sun-Jin;Kim, Jae Soon;Ryu, Doo Young
Journal of People, Plants, and Environment
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v.22
no.4
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pp.355-364
/
2019
This study was conducted to find out the effects of agro-healing activities and strolling on the physiological conditions of patients with chronic metabolic diseases. A total of 11 subjects participated in agro-healing activities, and their average age was 49.6±11.3. Changes in their salivary cortisol were compared and analyzed before and after participating in the agro-healing activity program, and it was found that the stress hormone decreased after participating in the program. Differences in brain wave between before and after participating in agro-healing activities and strolling were measured, and the ratio of alpha to high beta (RAHB) of subjects increased in most of the measured points after participating in agro-healing activities. In addition, the increase in the value in the temporal lobe (T3) that controls mortor skills and occipital lobe (O2) that controls visual functions indicates that the activities were effective in improving relaxation and stability. The relative low beta (RLB) power spectrum of subjects also statistically significantly decreased in the right occipital lobe (O2) that controls visual functions, the relative mid beta (RMB) and relative high beta (RHB) power spectrum decreased in the left temporal lobe (T3) and the right occipital lobe (O2). These results indicate that the activities were effective in improving relaxation under no stress and tension. The systolic and diastolic blood pressure of subjects after participating in agro-healing activities decreased from the prehypertension level to the normal level. These results indicate that horticultural activities and visual elements in healing farms, such as agro-healing activities and strolling, have positive impacts on patients with chronic metabolic diseases accompanied with diabetes or high blood pressure by increasing the brain activity and psycho-physiological conditions of participants.
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