• Journal of Pharmaceutical Investigation
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• 제19권4호
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• pp.191-194
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• 1989

Bioequivalence test of $Asthcontin^{\circledR}$ tablet, a commercial slow-release theophylline (TP) dosage form, was performed using $Slo-bid^{\circledR}$ capsule as the reference. Since it has been confirmed that the saliva concentration of TP is closely correlated with the plasma concentration in man, the area under the saliva concentration-time curve was used as a bioavailability parameter. The statistical analysis showed that the two dosage forms are equivalent in bioavailability estimating from the saliva concentration. The results supported that the use of soliva as a test sample provides simple and easy techniques for bioequivalence tests of TP-containing dosage forms.

• 한국치위생학회지
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• 제17권2호
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• pp.283-294
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• 2017

Objectives: The purpose of the study was to investigate the effect of calcium concentration in saliva on dental caries activity after consuming calcium. Methods: A total of 59 adult women aged 20 to 40 years were surveyed for calcium intake. The daily average calcium intake was analyzed through dietary records of the subjects. The subjects were divided into two groups based on daily average calcium intake. Salivary pH and concentrations of minerals in the saliva were obtained from A group and B group. Calcium ($Ca^{2+}$) and magnesium ($Mg^{2+}$) concentrations in saliva were measured by HPLC-Ion chromatography using 15 mM sulfuric acid. The dental caries activity test was quantified by salivary buffer capacity test and plaque pH test. Results: The mean $Ca^{2+}$ concentrations of A group was $12.75{\mu}g/m$, the mean $Ca^{2+}$ concentrations in the B group was $16.30{\mu}g/mL$ (p<0.05) and respectively, $Mg^{2+}$ concentrations were found to be $0.48{\mu}g/mL$ and $0.51{\mu}g/mL$. Calcium intake and calcium concentration in saliva showed a significant correlation (r=0.380). Conclusions: The mean $Ca^{2+}$ concentrations in saliva was higher in the high calcium intake group. Therefore, calcium intake in saliva was correlated with dental caries.

• 치위생과학회지
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• 제21권3호
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• pp.150-157
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• 2021

Background: Stress as a cause of mental health problems is known to be more prevalent in women than in men and has a negative effect on several aspects of physical health, such as the composition of blood and saliva. This study investigated the relationship of perceived stress with blood cell counts, saliva flow rate, and saliva factors. Methods: We recruited women in their 20s with a high prevalence of stress. Stress was evaluated using the Korean version of the perceived stress scale. Blood tests included white blood cell, hemoglobin, and platelet. We then examined the saliva flow rate and cariogenic bacteria level, acidity, occult blood, buffer capacity, leukocyte level, protein level, and ammonia level using rinse water with the SILL-Ha^Ⓡ saliva test system. Results: In a total of 70 participants, the average age was 21.64 years old, the average perceived stress score was 16.96±4.32, and high levels of stress were reported by 80% of the participants (n=56). The high-stress group had lower hemoglobin levels. In addition, the high-stress group showed a lower saliva flow rate than the low-stress group, and there was a difference in the salivary acidity and buffer capacity. The total perceived stress score showed a positive correlation with acidity and negative correlation with buffer capacity and the hemoglobin level. Conclusion: This study found that stress in female college students might affect the composition of blood and saliva. High levels of stress were positively correlated with the hemoglobin level, saliva flow rate, and acidity and negatively correlated with the buffer capacity.

• Journal of Periodontal and Implant Science
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• 제40권4호
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• pp.164-171
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• 2010

Purpose: This study compared the total antioxidant status (TAS) and superoxide dismutase (SOD) activity in the saliva of periodontally compromised patients before and after scaling and root planing (SRP) to assess their diagnostic utility. Methods: Severe chronic periodontitis patient (test group) and subjects with no attachment loss, sites showing a 3 mm or more probing depth and a sulcus bleeding index < 10% (control group) were enrolled in this study. Saliva sampling and clinical examination were performed at one week, one month and 3 months after SRP. The TAS and SOD activity in each patient's saliva was measured for the comparative analysis between the groups. Results: In the test group, the TAS decreased directly after SRP. With time, it increased slightly and was relatively unchanged compared to the baseline. In the control group, the TAS also decreased immediately after SRP but increased gradually with time until 3 months. The SOD activity in the test and control subjects decreased immediately after SRP until 1 month. At 3 months, the SOD activity had increased. Both groups had a similar profile of SOD activity. However, the SOD activity of the control group was significantly higher than that of the test group at each point in time (P < 0.05). Conclusions: There was a significant difference in the total salivary antioxidant level between the periodontitis and healthy or gingivitis (control) group during the experiment period. The total antioxidant level in the saliva was higher in the patients with severe chronic periodontitis than the healthy or gingivitis control before SRP. The SOD activity of the periodontitis patients was lower than the control at each time point. These findings conclusively reveal the possible use of saliva as a diagnostic tool for periodontal health.

• 대한소아치과학회지
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• 제44권3호
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• pp.298-305
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• 2017

본 연구는 임상에서 사용되는 진단도구들이 소아에서 우식위험도와 상관관계를 가지는지 여부를 확인하고, 소아에서 우식위험도를 효과적으로 반영하는 지표가 무엇인지 알아보기 위함이다. 32명의 6세 미만의 소아를 두 군(I군 : dmft < 6, II군 : dmft > 6)으로 나누었다. Plaque-check PH을 이용해 치태의 pH를 측정하고, Saliva-check buffer를 이용해 타액의 pH와 완충능력를 측정하였다. S. mutans 검출을 위해 Saliva-check mutans와 Cytoperio analysis system을 이용하였다. 타액 완충능력은 두 군에서 유의한 차이를 보이며 dmft와 상관관계를 보였고, 타액의 pH와도 유의한 상관관계가 있는 것으로 확인되었다. 두 군 사이에 치태 pH, 타액 pH의 유의한 차이는 관찰되지 않으며 dmft와 상관관계를 가지지 않는 것으로 밝혀졌다. S. mutans의 단일클론항체를 이용하는 Saliva-check mutans와 real-time PCR에 기반한 Cytoperio analysis system은 dmft와 유의한 상관관계를 가지고 있지 않는 것으로 밝혀졌다.

• 아동학회지
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• 제33권3호
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• pp.83-97
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• 2012

The purpose of this study is to investigate the effects of sand play therapy on the parenting stress and saliva cortisol levels of parents undergoing child counseling programs. The study was conducted with 13 parents who were undergoing child counseling programs. The sessions were held every week for 45 minutes from July, 2011 to September, 2011. To evaluate the effects of sand play therapy, pre test and post test were conducted and the results were then analyzed. The therapy sessions consist of parents making sand boxes, and this was done without consideration of any particular theme. The instrument used was Abidin (1990)'s Parenting Stress Instrument (PSI). Saliva cortisol levels were obtained at the pre-post stage of the sand play therapy. The data were analyzed by means of frequency and the Wilcoxon Rank Sum Test was conducted by the SPSS. The major findings were as follows; There were significant differences in terms of the decreases in the areas of perception of all the parenting stresses examined, including daily life stress, child temperament stress, child relationship stress, and learning-expectation stress, as well as in saliva cortisol levels. Finally, this study suggests that parenting stress and saliva cortisol levels have a positive relationship, and the effects of sand play therapy were significant with decreases in parenting stress and saliva cortisol levels. There is a clear need for parents undergoing child counseling programs to engage in sand play therapy to decrease parenting stress and saliva cortisol levels.

• 분석과학
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• 제21권1호
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• pp.48-52
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• 2008

본 연구에서는 사건 현장 증거물에서 타액반의 확인을 위해 개발된 $SALIgAE^{(R)}$ 시약의 유효성을 검토하였다. $SALIgAE^{(R)}$ 검사법의 상세한 작용 기작에 대해서는 상업적 이유 등으로 잘 알려져 있지 않아 실험을 통해 민감도와 특이성을 검토하였으며, 이를 기존의 타액검사 방법인 아가로스 겔 확산법 및 $Phadebas^{(R)}$ 검사법과 비교하였다. 사건 현장에서 경제적이며 쉽고 신속하게 타액검사를 수행할 수 있도록 $SALIgAE^{(R)}$검사법을 변형하였는데, 5분 이내에 1/600 이상 희석된 타액까지 확인이 가능하였다. 타액 이외의 인체 분비물(정액, 질액, 뇨, 땀, 콧물)은 5분 이내에 $SALIgAE^{(R)}$ 검사 양성반응을 보이지 않았다. 또한 $SALIgAE^{(R)}$ 검사 시약은 상온에서도 높은 안정성을 보여 법과학 실험실에서는 물론 사건 현장에서도 유용하게 사용할 수 있을 것으로 판단되었다.

• 한국치위생학회지
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• 제3권2호
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• pp.197-208
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• 2003

This study was implemented for 84 students of dental hygiene to show the correlation between dental caries experience and improved caries activity test. Dental caries experience for the sample groups was examined and stimulative saliva secreted for 5 minutes was collected into the tube to check saliva secretion rate. Dentocult LB test was executed to observe Lactobacilli colonies after 96 hour cultivation of culture slides moistened with stimulative saliva. Dentocult SM test(screening strip, site strip) was done to measure SM colonies distribution after 48 hour cultivation of culture strips applied with collected saliva and dental plaque respectively, and salivary buffering capacity was checked by means of Dentobuff strip kit. Following conclusions are obtained after examining the relation between Dentocult LB, Dentocult SM, Dentobuff strip test results and DMFT index, salivary secretion rate. 1. Showed no significant difference between Dentocult LB test results and DMFT index, salivary secretion rate. 2. Showed no significant difference between Dentocult SM(screening strip) test results and DMFT index, salivary secretion rate. 3. Showed significant difference between Dentocult SM(site strip) test results and DMFT index(pE0.05), but showed no significant difference between Dentocult SM(site strip) test results and salivary secretion rate. 4. Showed no significant difference between Dentobuff strip test results and DMFT index, but showed a very wide difference between Dentobuff strip test results and salivary secretion rate(pE0.01).

• 대한소아치과학회지
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• 제34권1호
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• pp.73-80
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• 2007

소아 환자의 인접면 우식을 치료할 때 컴포머는 불소를 방출하는 재료로 유용하게 사용될 수 있다. 하지만 구강내 환경은 항상 습윤한 상태로 타액은 컴포머와 치질 사이의 접착을 방해할 수 있다. 수복시에 타액이 게재하게 되면 미세누출이 발생할 가능성이 높아지게 되며 그로 인해서 수복의 실패가 일어날 수 있다. 이에 본 연구에서는 컴포머를 수복할 시에 타액의 영향과 수복 방법에 따른 미세누출 정도를 평가하기 위해서 시행하였다. 컴포머로서 Dyract $AP^{(R)}$(Dentsply, Germany)를 사용하였으며 Dentin bonding agent로는 Prime and $Bond^{(R)}$ NT (Dentsply, Germany)를 사용하였고, 광중합을 위해서 Elipar Trilight (3M ESPE, USA)를 사용하였다. 구강내 환경을 재현하기 위해서 saliva pool을 제작하였다. 소구치 2개를 인접하여 시편을 제작한 후에 2급 와동을 형성하여 수복 환경을 다르게 하여 컴포머를 충전한 후에 500회 thermocycling하였다. 그 후 0.5% methylene blue 용액에 24 시간 동안 담근 후에 실체 현미경을 통해서 교합면과 치은면에서의 미세누출 정도를 측정하였다. Kruskal-Wallis Test와 Mann-Whitney Test를 이용하여 각 군간 유의성을 검정하여 다음과 같은 결과를 얻었다. 1. 교합면에서의 각 군간 통계학적 차이는 없었다(p>0.05). 2. 치은면에서 $Oraseal^{(R)}$을 이용하여 수복한 3군이 타액 오염을 시키지 않은 4군과 통계학적 차이가 없었다(p>0.05). 3. 치은면에서 1군과 2군 사이에는 통계학적 차이가 없었다(p>0.05). 4. 치은면에서 $Oraseal^{(R)}$을 이용하여 수복한 3군이 1, 2군보다 통계학적으로 더 낮은 미세누출 정도를 보였다(p<0.05).

• Restorative Dentistry and Endodontics
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• 제25권1호
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• pp.46-55
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• 2000

During bonding procedure of composite resin, the prepared cavity can be contaminated by saliva. In this study, marginal microleakage and shear bond strength of a composite resin to primed enamel and dentin treated with artificial saliva(Taliva$^{(R)}$) were evaluated. For the marginal microleakage test, Class V cavities were prepared in the buccal surfaces of fifty molars. The samples were randomly assigned into 5 groups with 10 samples in each group. Control group was applied with a bonding system (Scotchbond$^{TM}$ Multi-Purpose plus) according to manufacture's directions without saliva contamination. Experimental groups were divided into 4 groups and contaminated with artificial saliva for 30 seconds after priming: Experimental 1 group ; artificial saliva was dried with compressed air only, Experimental 2 group ; artificial saliva was rinsed and dried. Experimental 3 group ; cavities were etched with 35% phosphoric acid for 15 seconds after rinsing and drying artificial saliva. Experimental 4 group ; cavities were etched with 35% phosphoric acid for 15 seconds and primer was reapplied after rinsing and drying artificial saliva. All the cavities were applied a bonding agent and filled with a composite resin (Z-100$^{TM}$). Specimens were immersed in 0.5% basic fuschin dye for 24 hours and embedded in transparent acrylic resin and sectioned buccolingually with diamond wheel saw. Four sections were obtained from one specimen. Degree of marginal leakage was scored under stereomicroscope and their scores were averaged from four sections. The data were analyzed by Kruscal-Wallis test and Fisher's LSD. For the shear bond strength test, the buccal or occlusal surfaces of one hundred molar teeth were ground to expose enamel(n=50) or dentin(n=50) using diamond wheel saw and its surface was smoothed with Lapping and Polishing Machine(South Bay Technology Co., U.S.A.). Samples were divided into 5 groups. Treatment of saliva-contaminated enamel and dentin surfaces was same as the marginal microleakage test and composite resin was bonded via a gelatin capsule. All specimens were stored in distilled water for 48 hours. The shear bond strengths were measured by universal testing machine (AGS-1000 4D, Shimaduzu Co., Japan) with a crosshead speed of 5 mm/minute. Failure mode of fracture sites was examined under stereomicroscope. The data were analyzed by ANOVA and Tukey's studentized range test. The results of this study were as follows : 1. Enamel marginal microleakage showed no significant difference among groups. 2. Dentinal marginal microleakages of control, experimental 2 and 4 groups were lower than those of experimental 1 and 3 groups (p<0.05). 3. The shear bond strength to enamel was the highest value in control group (20.03${\pm}$4.47MPa) and the lowest value in experimental 1 group (13.28${\pm}$6.52MPa). There were significant differences between experimental 1 group and other groups (p<0.05). 4. The shear bond strength to dentin was higher in control group (17.87${\pm}$4.02MPa) and experimental 4 group (16.38${\pm}$3.23MPa) than in other groups, its value was low in experimental 1 group (3.95${\pm}$2.51 MPa) and experimental 2 group (6.72${\pm}$2.26MPa)(p<0.05). 5. Failure mode of fractured site on the enamel showed mostly adhesive failures in experimental 1 and 3 groups. 6. Failure mode of fractured site on the dentin did not show adhesive failures in control group, but showed mostly adhesive failure in experimental groups. As a summary of above results, if the primed tooth surface was contaminated with artificial saliva, primer should be reapplied after re-etching it.