• 대한한의진단학회지
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• 제23권1호
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• pp.1-8
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• 2019

Objectives The purpose of this study is to determine if there is a difference in acupuncture sensation depending on the bee venom pharmacopunctures. The study was designed to identify the intrinsic acupuncture sensation of the bee venom pharmacopunctures compared to saline. Methods Bee venom,(BV), Sweet bee venom(SBV), and normal saline were injected in order to the left of ST36 (Joksamni), ST37 (Sang-geoheo), right ST36 (Joksamni), ST37 (Sanggeoheo) each. The order of insertion of the BV, SBV, and normal saline was randomly assigned using a computerized random number table. The questionnaire used in this study was based on the Massachusetts General Hospital Acupuncture Sensation Scale (MASS). Results : BV and SBV was statistically significantly higher than saline in soreness, aching, distention, sharp pain and itchiness. Above this, BV was statistically significantly higher than saline in tingling and throbbing. And SBV was statistically significantly higher than saline in warmth. BV was statistically significantly higher than SBV in ichiness. Conclusion. BV and SBV were mainly strong, heavy, and gave a sharp feeling to the subjects. The results of the study can be used as references for future bee venom pharmacopunctures use. In addition, the results of the study can be used as basic data for clinical trials using bee venom pharmacopunctures.

• 핵의학기술
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• 제14권2호
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• pp.128-132
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• 2010

핵의학과 종합조작실은 방사선 관리구역으로 이곳에서 발생된 방사성오염물질은 방사성폐기물로 간주하여 일반쓰레기와 별도로 관리를 해야 한다. 본 실험은 우리가 미처 인식하지 못해 일반쓰레기로 처분되었던 방사성오염물질의 오염도를 측정하고 원인을 분석하여 보다 적극적인 방사성폐기물 관리 운영의 필요성을 제시하고자 함이다. 측정대상은 방사성의약품을 표지하기 위해 사용된 생리식염수와 $^{99}Mo$/$^{99m}Tc$ generator(국산 및 외산)에서 발생한 generator cap, saline needle cap, $^{99m}Tc$-needle cap, saline vial로 하였고, gamma survey meter를 이용하여 평균값과 표준 편차(mean${\pm}$SD)로 나타냈다. 측정대상물질의 오염유무를 알기 위해 방사선 관리구역에서 반출될 수 있는 물질의 표면오염도인 최대허용표면오염도의 1/10(43.2 cpm)을 기준값으로 정하였다. 각각의 표면오염도를 측정한 결과 방사성의약품 표지를 위해 사용된 생리식염수에서는 $14,429{\pm}26,378$ cpm으로 최대 허용표면오염도의 1/10을 초과하였다. 그리고 측정된 generator중 외산에서는 generator cap: $17{\pm}28$ cpm, saline needle cap: $35{\pm}66$ cpm, $^{99m}Tc$-needle cap: $9{\pm}21$ cpm, saline vial: $13{\pm}28$ cpm으로 최대허용표면오염도의 1/10를 초과하지 않았지만 국산에서는 generator cap: $22,852{\pm}52,545$ cpm, saline needle cap: $87,367{\pm}109,711$ cpm, $^{99m}Tc$-needle cap: $9,008{\pm}10,459$ cpm, saline vial: $186,416{\pm}158,196$ cpm으로 최대 허용표면오염도의 1/10를 초과하였다. 외산 generator에서 발생한 측정대상물질은 일반폐기물로 처분하고 국산 generator와 방사성의약품을 표지하기 위해 사용된 생리식염수는 반드시 방사성폐기물로 간주하여 처분해야 한다. 따라서 일반쓰레기 뿐만 아니라 종합조작실에서 반출되는 모든 물질에 대한 자체적인 방사선측정, 기록 및 비치와 방사성폐기물에 대한 지속적인 교육을 통해 방사성 폐기물이 일반쓰레기로 처분되어 나가는 것을 예방 할 수 있을 것이다.

• 대한한의학회지
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• 제20권1호
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• pp.185-196
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• 1999

The aim of the present study was to investigate the effect of Hwangryeonheadock-Tang and Onchung-Eum on essential hypertension and hyperlipidemia. Rats were orally administered for 30days with Hwangryeonheadock-Tang and Onchung-Eum and the blood was withdrawn at 10, 20 and 30days after an oral administration. The heart rate, tail blood pressure, plasma renin activity, plasma level of aldosterone. catecholamine, sodium and angiotensin II were measured after an oral administration of Hwangryeonheadock-Tang and Onchung-Eum in spontaneously hypertensive rat(SHR). In addition, serum levels of total cholesterol, triglyceride, HDL-cholesterol. LDL-cholesterol and total lipid were measured cholesterol-fed rats. The results were summarized as follows ; 1. A significant decrease of tail blood pressure was shown at 10, 20 and 30days after Hwangryeonheadock-Tang and Onchung-Eum treatment in SHRs. compared with saline. 2. Heart rate was significantly decreased at 30days in SHRs after Hwangryeonheadock-Tang treatment and at 20, 30days after Onchung-Eum treatment in SHRs. compared with the effects of saline group. 3, A significant decrease of plasma aldosterone level was elicited at 10, 20days after Hwangryeonheadock-Tang treatment in SHRs, compared with the effects of saline group, 4. Plasma renin activity was significantly decreased at 10days after Onchung-Eum treatment compared with the effects of saline group in SHRs. 5. Plasma norepinephrine level was significantly decreased at 20 and 30clays after Onchung-Eum treatment in SHRs, compared with the effects of saline group. 6. A significant decrease of plasma epinephrine level was induced at 30days after Hwangryeonheadock-Tang treatment and at 10, 20 and 30days after Onchung-Eum treatment, compared with the effects of saline group in SHRs. 7. Plasma sodium level was. significantly decreased at 20days after Hwangryeonheadock-Tang and Onchung-Eum treatment, compared with the effects of saline group in SHRs. 8. Plasma angiotensin II level was significantly decreased at 30days after Onchung-Eum treatment, compared with the effects of saline group in SHRs. 9. A significant decrease of body weight was observed at 20 and 30days after Hwangryeonheadock-Tang treatment and at 10, 20 and 30days after Onchung-Eum treatment. compared with the effects of saline group in hyperlipidemia rats. 10. Hwangryeonheadock-Tang and Onchung-Eum showed a significantly decreasing effect at 30days on serum total cholesterol level in hyperlipidemia rats, compared with the saline treatment. 11. Hwangryeonheadock-Tang and Onchung-Eum saw 20 and 30days respectively on serum triglyceride level in the saline treatment. 12. Hwangryeonheadock-Tang and Onchung-Eum decreased on serum HDL-cholesterol level significantly, compared with the saline treatment in hyperlipidemia rats. 13. A significant decrease of serum LDL-cholesterol was observed at 10 and 30days after Hwangryeonheadock-Tang treatment and at 30days after Onchung-Eum treatment, compared with the effects of saline group in hyperlipidemia rats. 14. Hwangryeonheadock-Tang had a significantly decreasing effect at 10, 20 and 30days on serum total lipid level, compared with the saline treatment in hyperlipidemia rats. 15. Hwangryeonheadock-Tang elicited a significantly decreasing effect on weight of kidneys, spleen and testes respectively and Onchung-Eum induced on weight of liver and spleen respectively in hyperlipidemia rats, compared with saline treatment. These Findings suggest a possible anti-hypertensive and anti-hyperlipidemic effect of Hwangryeonheadock-Tang and Onchung-Eum.

• 한국작물학회지
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• 제52권2호
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• pp.204-207
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• 2007

서남부 간척지에서 벼 담수표면직파재배시 토양 염농도 별 파종전 후 합리적인 물관리 방법을 구명하고자 $2004{\sim}2005$년에 걸쳐 호남농업연구소 계화도출장소 시험포장인 세사양토(문포통)에서 남평벼를 공시하여 로타리 후 환수횟수와 파종 후 물관리 방법을 각기 달리하여 입모 및 쌀 수량등을 검토한 결과를 요약하면 다음과 같다. 1. 저염토양에서는 로타리 후 1회 환수, 파종 후에는 낙수 관리해 주어야 입모가 양호하고 쌀 수량이 감소되지 않았다. 2. 중염토양에서는 로타리 후 3회 환수, 파종 후에는 담수관리 하되 2일 간격으로 환수를 실시해 주어야 입모가 양호하고 쌀 수량감소가 적었다. 3. 따라서 서남부 간척지에서 벼 담수표면직파재배시 입모 및 쌀 수량 등을 고려할 때 저염답에서는 로타리 후 1회 환수, 파종 후에는 낙수관리, 중염답에서는 로타리 후 3회 환수, 파종 후에는 담수관리하되 2일 간격으로 환수하는 것이 유리하다.

• The Korean Journal of Physiology
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• 제8권1호
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• pp.15-22
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• 1974

The influence, upon male rat's mating behavior, of Korean Panax Ginseng administered for 3 and 5 days was investigated by direct behavioral observation and also by counting the number of copulation plugs the animals deposited. Four쇼-four male albino rats were used. Part of the animals received ginseng for 3 days (ginseng-3 day group, N= 12) or for 5 days(ginseng-5 day group, H=10), while the remaining animals received saline for 3 days (saline-3 day group, N=12) or for 5 days (saline-S day group, N=10). Each animal belonging to the 2 ginseng groups received subcutaneously 0.5 ml/100 g body weight of ginseng alcohol extract solution (4 mg of the ethyl alcohol extract in 1 ml of physiological saline), and each rat belonging to the 2 saline groups received the same amount of saline per day. During the dark period of the light-dark cycle on the next day following the last drug administration, a female rat in the artificial estrus was introduced to each male and the mating behavior was observed for 45 minutes. The observation session was divided into two parts and, in the early part which terminated with the first ejaculation and succeeding intromission, following behavioral measures were taken: mounting latency, intromission latency, inter-intromission period, ejaculatory latency(time from the first intromission until the first ejaculation), occurrence of mounting with intromission, occurrence of mounting without intromission, and postejaculatory interval. Behavioral measures taken in the later part of the session after the first ejaculation were: occurrence of mounting with intromission, occurrence of mounting without intromission, and occurrence of ejaculation. Immediately after the behavioral observation session the experiment turned to measure, for 10 days, the number of copulation plug which each pair of rats deposited. Following results were obtained: 1. After several mountings mounting with intromission, males of the 2 ginseng groups finished the first ejaculation significantly earlier than the corresponding 2 saline groups did. 2. The postejaculatory latency was significantly reduced in the ginseng-5 day group compare with the value of the saline-5 day group and also compared with the value of the ginseng-3 day group. 3. The 2 ginseng groups ejaculated significantly more often in 45 minutes' observation session than the corresponding 2 saline groups did. 4. The number of copulation plug deposited in 10 days by the animals of the 2 ginseng groups. significantly exceeded the number deposited by the corresponding 2 saline group animals. The animals of the ginseng-5 day group deposited copulation plugs significantly more than the animals of the ginseng-3 day group did. It is inferred from the above results that the ginseng facilitates mating behavior of male rats, and that the degree of facilitation may be influenced by the duration of drug administration.

• Journal of Forest and Environmental Science
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• 제28권1호
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• pp.12-18
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• 2012

Like any other natural resources, forest flora may experience the extreme threat of elevated temperature and saline water submergence at different stages of their lives i.e. from germination to maturity due to climate change effects. The overall aim of the study was to measure the effect of higher temperatures along with saline water irrigation on survival and initial growth during seedling stage of Artocarpus chapalasha. The experiment was conducted in temperature- humidity-photoperiod regulated plant growth chamber during stipulated period to measure the growth performance of randomly selected seedlings. Within three different elevated temperatures viz. $30^{\circ}C$, $32^{\circ}C$ and $34^{\circ}C$, the seedlings were given three different saline conditions such as 0.5 g/L, 1.5 g/L and 2.5 g/L NaCl concentrations. Results found from the experiment was that, seedlings of Artocarpus chaplasha reared at different temperatures and saline water treatments showed stunted growth than reared at existing outdoor temperature ($26.31^{\circ}C$) irrigated with regular fresh water. Seedling growth at three different parameters such as height, collar diameter and number of leaves showed that with increasing temperature individuals respond negatively to increasing saline condition. The seedling's growth occurred at every day in height, collar diameter and leaf. However, growth rate reduced later during the observation. The combined effect of high salinity and higher elevated temperature results in seedling mortality. Therefore, Artocarpus chaplasha may not thrive at higher temperature and salinity intrusion at its early growing period in plantation and natural forest areas.

• The Korean Journal of Pain
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• 제27권4호
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• pp.345-352
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• 2014

Background: Epidural administration of dexamethasone has been suggested for pain control after minor orthopedic surgery. This study was conducted to assess its efficacy after such surgery, combined or not to IV dipyrone, IV parecoxibe or their combination. Methods: 91 patients were randomly assigned to seven groups. Patients were submitted to spinal bupivacaine anesthesia combined to epidural administration of either 10 ml saline or 10 mg dexamethasone diluted to 10-ml volume. Patients also received 10 ml IV saline or 1 gr dipyrone and/or 40 mg parecoxibe diluted to 10 ml with saline. Control group (CG) received epidural and IV saline. Dexamethasone group (DexG) received epidural dexamethasone and IV saline. Dipyrone group (DipG) received epidural saline and IV dipyrone. Dex-Dip G received epidural dexamethasone and IV dipyrone. Parecoxibe group (ParG) received epidural saline and IV parecoxibe. Dex-ParG received epidural dexamethasone and IV parecoxibe. Finally, Dex-Dip-ParG received epidural dexamethasone and IV dipyrone plus IV parecoxibe. Results: The CG expressed 4h of analgesia and sooner requested pain killer. DexG was similar to DipG or ParG or Dex-ParG (7-hours), and they requested less ketoprofen compared to the CG (P < 0.05). However, the Dex-DipG and the Dex-Dip-ParG resulted in longer time to demand pain killer (17-hours) and less ketoprofen consumption in 24-hours (P < 0.002). Adverse effects were similar among groups. Conclusions: The analgesia secondary to epidural dexamethasone was enhanced by IV dipyrone, while no effects were observed by the addition of IV parecoxibe.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.435-440
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• 2013

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 ${\mu}l$ per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

• 한국콘텐츠학회논문지
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• 제20권6호
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• pp.439-444
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• 2020

이 연구는 자기공명영상검사 시 조영제 주입용으로 사용되는 자동주입기에서 조영제 역류에 의한 생리식염수의 오탁 현상을 방지할 수 있는 대안을 모색하고자 하였다. 연구방법은 실린지 내에서 조영제 역류를 차단할 대안으로 밸브를 자체 제작한 다음, 임상적용의 유용성을 알아보기 위해 생리식염수의 상태를 세 그룹으로 나누고 조영제 주입을 실험하여 각각 채취한 생리식염수의 지방소거 T1 강조영상으로 신호강도를 비교하였다. 연구결과, 그룹 1의 조영제 주입 전 생리식염수와 그룹 3의 역류방지용 밸브를 적용한 조영제 주입 후 생리식염수가 통계적으로 유의한 차이가 없었다(p>0.05). 이는 자체 제작한 역류방지용 밸브가 조영제 주입 시 생리식염수로 역류하는 조영제를 완벽히 차단한 것으로 분석된다. 결론적으로 조영제 자동주입기를 활용한 자기공명영상 동적검사 시 본 연구에서 제시한 역류방지밸브를 적용한다면 조영제 역류로 인한 생리식염수의 오탁 현상을 예방할 수 있으리라 본다.

• 한국식품과학회지
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• 제48권4호
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• pp.335-340
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• 2016

본 연구에서는 다양한 식품으로부터 식중독 세균의 DNA를 추출하는 효율을 비교 하였다. 사용된 DNA 추출 방법은 TaKaRa Kit를 이용하는 방법, PrepMan reagent를 이용하는 방법, boiling method, PEG를 이용한 alkaline method가 사용되었다. 비용절감이나 시간절약 면에서 boiling method나 PrepMan method도 고려할 수 있지만, column kit를 이용하는 TaKaRa kit가 효율적이라고 판단되었다. 또한 정성 시험법에서 적은 양의 균을 검출하기 위해 증균배양을 거치게 되는데 이때 사용되는 증균배지의 성분이 DNA 추출 후에도 잔류하여 saline과 비교하였을 때 DNA 추출효율이 낮은 결과를 나타내었다. 따라서 증균배지의 성분을 제거한 뒤 DNA를 추출하는 것이 PCR의 효율을 높일 수 있을 것으로 예상된다. 정량 시험법에서는 증균과정을 거치지 않아 균을 검출할 때 DNA의 추출 효율이 중요하기 때문에 DNA 추출 효율을 높이기 위한 가장 좋은 버퍼를 선정하고자 하였다. 그 결과 E. coli O157:H7에서는 saline이, S. aureus에서는 증류수를 버퍼로 사용했을 때 DNA 추출 효율이 가장 높은 것으로 나타났다.