• 한국환경농학회지
• /
• 제32권3호
• /
• pp.207-213
• /
• 2013

Thidiazuron은 1976년 Plant Physiology 발표를 통해 생장조정제로 알려진 이후, 현재 우리나라에서도 생장조정제로 수박과 참외의 착과 증진 및 참다래의 과실비대 촉진, 포도의 과립 비대 증진 및 착립 증진 등을 위해 등록되어 사용되고 있다. Thidiazuron의 분석법은 식품공전에 수재되어 있으나, 등재 당시의 기술 이용, 일부 시험 대상 품목에 대한적용 등으로, 최근의 잔류허용기준 설정 품목의 증가와 분석기기 및 기술 발달을 반영하여 검증된 분석법으로 개선코자 하였다. 더불어, 개발된 분석법을 활용하여, thidiazuron의 사용이 허가되어 있는 수박, 키위, 포도를 포함한 국민 다소 비 식품 17품목에 대한 잔류실태조사도 수행하였다. 개발된 분석법의 회수율은, 현미 89.2~91.2 %, 고추 87.2~92.1 %, 감자 76.4~86.9 %, 수박 91.2~95.7 %, 키위 86.5~88.5 %, 포도 89.5~94.0 % 였으며, 반복 회수율 간 변이계수는 10 % 이하였다. 또한 개발된 분석법으로 우리나라에서 유통되고 있는 다소비 농산물 쌀, 상추, 사과 등 17품목 358건에 대해 농약 잔류실태를 조사한 결과 모든 시료에서 thidazulon은 검출되지 않았다.

• International Journal of Safety
• /
• 제6권2호
• /
• pp.33-37
• /
• 2007

In recent days, the accidents have happened at experimental laboratories in universities or institutes. In order to improve safety assurance of workers in laboratories, it is required to carry out systematic study concerned with evaluation of safety and health level. The safety and health inspection checklists were developed and conducted the case study. The case study was carried out laboratory safety inspection with 5 inspectors by the checklists to become aware of safety and health level. From the evaluation results of the inspection, we could be made quantification of laboratory through evaluation of safety and health level in laboratory. It was found that was recognized present states of the laboratory and established a plan for improvements in laboratory safety facilities from the case study. The safety inspection checklists can be used as basic data to establish evaluation criteria of safety and health level.

• Biomolecules & Therapeutics
• /
• 제25권3호
• /
• pp.266-271
• /
• 2017

Synthetic cannabinoids are one of most abused new psychoactive substances. The recreational use of abused drug has aroused serious concerns about the consequences of these drugs on infection. However, the effects of synthetic cannabinoid on resistance to tetanus toxin are not fully understood yet. In the present study, we aimed to determine if the administration of synthetic cannabinoids increase the susceptibility to tetanus toxin-induced motor behavioral deficit and functional changes in cerebellar neurons in mice. Furthermore, we measured T lymphocytes marker levels, such as CD8 and CD4 which against tetanus toxin. JWH-210 administration decreased expression levels of T cell activators including cluster of differentiation (CD) $3{\varepsilon}$, $CD3{\gamma}$, CD74p31, and CD74p41. In addition, we demonstrated that JWH-210 induced motor impairment and decrement of vesicle-associated membrane proteins 2 levels in the cerebellum of mice treated with tetanus toxin. Furthermore, cerebellar glutamatergic neuronal homeostasis was hampered by JWH-210 administration, as evidenced by increased glutamate concentration levels in the cerebellum. These results suggest that JWH-210 may increase the vulnerability to tetanus toxin via the regulation of immune function.

• Toxicological Research
• /
• 제31권3호
• /
• pp.221-226
• /
• 2015

With the diversification and internationalization of the food industry and the increased focus on health from a majority of consumers, food safety policies are being implemented based on scientific evidence. Risk analysis represents the most useful scientific approach for making food safety decisions. Total diet study (TDS) is often used as a risk assessment tool to evaluate exposure to hazardous elements. Many countries perform TDSs to screen for chemicals in foods and analyze exposure trends to hazardous elements. TDSs differ from traditional food monitoring in two major aspects: chemicals are analyzed in food in the form in which it will be consumed and it is cost-effective in analyzing composite samples after processing multiple ingredients together. In Korea, TDSs have been conducted to estimate dietary intakes of heavy metals, pesticides, mycotoxins, persistent organic pollutants, and processing contaminants. TDSs need to be carried out periodically to ensure food safety.

• Safe Food
• /
• 제5권1호
• /
• pp.26-35
• /
• 2010

• 한국식품과학회지
• /
• 제46권1호
• /
• pp.7-12
• /
• 2014

Flutianil은 흰가루병에 높은 방제효과를 가지는 thiazolidine계 살균제로서 고추, 피망, 오이, 참외에 대해 flutianil의 사용등록이 신청됨에 따라 농산물 중 flutianil 잔류량을 측정하기 위한 검사법을 개발하였다. Flutianil 잔류물은 acetonitrile로 추출하여 포화식염수와 dichloromethane을 이용한 액-액분배과정을 수행하였고, silica 카트리지를 이용하여 clean-up을 실시한 후 GC-ECD를 통해 기기분석을 수행하였으며, GC-MS로 재확인하였다. flutianil 표준품의 직선성은 상관계수($R^2$)가 0.999이상으로 우수하였고 고추, 피망, 감귤, 감자, 현미, 대두에 대한 flutianil의 회수율을 측정한 결과, 76.5-108.0%의 범위로 10% 미만의 표준오차를 나타내었으며, 분석법의 검출한계는 0.004 mg/kg, 정량한계 0.02 mg/kg이었다. 이는 코덱스 가이드라인(CAC/GL 40)인 회수율 70-120%, 분석오차 10 미만에 적합함을 보여주어 분석법의 정확성을 확인하였다. 본 연구에서 개발한 시험법은 flutianil 분석을 위한 신속, 정확한 시험법으로, 국내에서 유통되는 농산물 중 flutianil 잔류물의 안전관리를 위한 공정시험법으로 활용될 예정이다.

• Bulletin of the Korean Chemical Society
• /
• 제32권12호
• /
• pp.4458-4461
• /
• 2011

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2008년도 춘계학술대회
• /
• pp.239-245
• /
• 2008

In this study has investigated and considered product planning methods at the existing product design process and being used the safety evaluation methods at the product planning stage, and has deduced the 16 safety evaluation elements to have to be considered for executing the safety evaluation at the product planning stage. When the safety evaluation is executed from the product planning stage, more safe products will be designed.

• Biomolecules & Therapeutics
• /
• 제23권6호
• /
• pp.597-603
• /
• 2015

Synthetic cannabinoids JWH-018 and JWH-250 in 'herbal incense' also called 'spice' were first introduced in many countries. Numerous synthetic cannabinoids with similar chemical structures emerged simultaneously and suddenly. Currently there are not sufficient data on their adverse effects including neurotoxicity. There are only anecdotal reports that suggest their toxicity. In the present study, we evaluated the neurotoxicity of two synthetic cannabinoids (JWH-081 and JWH-210) through observation of various behavioral changes and analysis of histopathological changes using experimental mice with various doses (0.1, 1, 5 mg/kg). In functional observation battery (FOB) test, animals treated with 5 mg/kg of JWH-081 or JWH-210 showed traction and tremor. Their locomotor activities and rotarod retention time were significantly (p<0.05) decreased. However, no significant change was observed in learning or memory function. In histopathological analysis, neural cells of the animals treated with the high dose (5 mg/kg) of JWH-081 or JWH-210 showed distorted nuclei and nucleus membranes in the core shell of nucleus accumbens, suggesting neurotoxicity. Our results suggest that JWH-081 and JWH-210 may be neurotoxic substances through changing neuronal cell damages, especially in the core shell part of nucleus accumbens. To confirm our findings, further studies are needed in the future.

• 분석과학
• /
• 제22권4호
• /
• pp.345-353
• /
• 2009

식품 중 불법적으로 첨가된 부정유해물질을 규명하기 위하여 HPLC/PDA를 이용하여 인삼성분을 함유한 것으로 선전하는 식품 원료를 조사하였다. 발기부전치료제로 사용되는 실데나필의 변형체인 홍데나필과 유사한 구조를 갖는 미지물질이 확인되어 분취 크로마토그래피 등을 이용하여 불순물을 제거한 다음 HPLC/MS/MS, NMR 등의 기기를 이용하여 그 구조를 규명하였다. HPLC/MS 분석결과 미지 물질이 480 m/z의 분자량을 갖는 물질임을 확인하였으며 LC/MS/MS 분석 결과 실데나필에서 특징적으로 검출되는 m/z인 311, 339, 353이 확인되어 발기부전치료제와의 구조적 유사성을 확인할 수 있었다. NMR 분석 결과 미지물질은 홍데나필과 같이 sulfonyl 기가 acetyl기로 치환된 것을 확인 할 수 있었으며 실데나필의 에틸피페라진기가 옥소에틸피페라진기로 치환된 구조를 갖는 물질임을 확인하여 그 이름을 옥소홍데나필로 명명하였다.