• Food Science of Animal Resources
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• v.41 no.1
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• pp.59-70
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• 2021

A method for simultaneous detection of fipronil (F) and its metabolites fipronil desulfinyl (FD), fipronil sulfide (FS), fipronil sulfone (FSO) in chicken eggs was applied and validated. It includes single-step, cheap, effective, rugged, safe-based method (SinChERS) for sample preparation and ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) for chemical analysis. Results suggested that formic acid enhanced the recovery of 4 target residues and 1% supplementation to acetonitrile gained higher recoveries than that of 5%. SinChERS integrated extraction and clean-up steps into one, with shorter time (1.5 h) to operate and higher recoveries (97%-100%) than HLB, Envi-Carb-NH2 and quik-easy-cheap-effective-rugged-safe method (QuEChERS), and it consumed the smallest volume of extracting solvent (10 mL) as QuEChERS. Quantitative analyses using external standard method suggested the linear ranges of 4 target compounds were 1-20 ㎍/L with R2 >0.9947. The limit of detection (S/N>3) and quantification (S/N>10) were 0.3 ㎍/kg and 1 ㎍/kg. Recoveries ranged from 89.0% to 104.4%, and the relative standard deviations (n=6) at 1, 10, and 20 ㎍/kg were lower than 6.03%. Thirty batches of domestic eggs (500 g each) were detected by the established SinChERS-based UHPLC-MS/MS and no target residues were detected in all samples. The method developed in this study is a rapid, sensitive, accurate and economic way for multi-residue analysis of fipronil and its metabolites in eggs.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.05a
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• pp.911-912
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• 2012

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2010.05a
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• pp.1271-1272
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• 2010

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.99-105
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• 2000

Cell-Cell interaction and the extracellular matrix (ECM) are belisved to play essential roles during in vitro culturing of primary hepatocytes in the control of differentiation and in the maintenance of tissue spcific functions. The objective of this study was to examine the effects of degree of cell-cell contact (DCC) on liver sperific function of rat promary hepatocytes. Hepatocyte aggregates with various with various degrees of cell-cell contantact, I. e., dispersed cell, longish aggregate, rugged aggregate, and smooth spheroid were obtained at 1, 5-6, 15-20, and 36-48 hrs, respectively in suspension cultures grown in spinner flasks embedded in Caalginate bead and collagen gel in order. The may result from mass transfer limitation and shear damage caused by agitation during aggregation. The rugged aggregate showed a higer viability and albumin secretion rate than the dispersed cells or the other aggregates. This result indicates the possible enhancement of a bioartificial liver's (BAL) performance using primary hepatocytes and the reduction in time to prepare a BAL through optimization of the immobilization time.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.1
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• pp.25-32
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• 2012

In this study, we present a gripping mechanism that is inspired by caterpillar's proleg. A caterpillar's proleg has planta that gives compliance to the proleg by greatly deforming its shape. In the bio-inspired gripper, the planta is implemented by flexure joints. The flexures buckle when end force and end moment is applied on the joint in opposite direction. Using this characteristic, the gripping structure is designed so that the flexure buckling can occur. Flexure buckling increases the region where gripping force is constant and this region leads to increasing in gripping range. At the same time, flexure buckling decouples all spines and therefore all spines can move differentially and independently. With this simple but effective mechanism, the bioinspire gripper can achieve adaptive gripping on rough and rugged surfaces. A prototype is built to demonstrate adaptive gripping on rough and rugged surfaces such as cement block, brick.

• Journal of the Korean Vacuum Society
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• v.4 no.S2
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• pp.64-68
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• 1995

Silicon films were deposited at $430^{\circ}C$ by remote plasma chemical vapor deposition(RPECVD) with a gas mixture of $Si_2H_6/SiF_4/H_2$. The silicon films deposited without and with $SiF_4$ were characterized using atomic force microscopy(AFM), transmission electron microscopy(TEM) and X-ray diffraction(XRD). Both silicon films have the same rugged surface morphology, but, the silicon film deposited with $SiF_4$ exhibits more rugged. The silicon film deposited without $SiF_4$ is amorphous, whereas the silicon film deposited with $SiF_4$ is polycrystalline with very small needle-like grains which are perpendicular to the substrate and uniformly distributed in the thickness of the film. The silicon film deposited with $SiF_4$ was found to have a preferred orientation along the growth direction with the<110> of the film parallel to the <111> of the substrate. The effect of $SiF_4$ during RPECVD was discussed.

• Korean Journal of Remote Sensing
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• v.5 no.2
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• pp.109-122
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• 1989

The ISODATA method, which is one of the most famous of the square-error clustering methos, has been applied to two Chungju multivariate data sets in order to evaluate the effectiveness of the regional lithological mapping. One is an airborne radiometric data set and the other is a mixed data set of the airborne radiometric and Landsat TM data. In both cases, the classification of the Bulguksa granite and the Kyemyongsan biotite-quartz gneiss are the most successful. Hyangsanni dolomitic limestone and neighboring Daehyangsan quartzite are also classified by their typical lowness of the radioactive intensities, though it is still confused with some others such as water-covered areas and nearby alluvials, and unaltered limestone areas. Topographically rugged valleys are also classified as the same cluster as above. This could be due to unavoidable variations of flight height and the attitude of the airborne system in such rugged terrains. The regional geological mapping of sedimentary rock units of the Ockchun System is in general confused. This might be due to similarities between different sediments. Considarable discrepancies occurred in mapping some lithological boundaries might also be due to secondary effects such as contamination or smoothing in digitizing process. Further study should be continued in the variable selection scheme as no absolutely superior method claims to exist yet since it seems somewhat to be rather data dependent. Study could also be made on the data preprocessing in order to reduce the erratic effects as mentioned above, and thus hoprfully draw much better result in regional geological mapping.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.552-561
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• 2019

In korea, debris-flow disasters are induced by typhoon and localized torrential rainfall annually. These disasters are particularly severe in the Korean national park due to its geomorphological characteristics. This study was conducted to analyze the landslide characteristics and forest environmental factors of landslide areas located in rugged mountain range in the Korean national park (Mt. Seorak, Mt. Jiri, and Mt. Sobaek). Overall, landslides occurred at 474 sites. The average area of the landslide scar among these sites was 1,212 ㎡. The average landslide sediment was 1,389 ㎥, average landslide length was 75 m, and the average width was 12.9 m. The landslides frequently occurred in regions with igneous rock and coniferous forest. In addition, slope gradient degree (31°-40°), slope gradient direction (N), vertical slope (concave), cross slope (concave), altitude (401-800 m), position (middle), stream order (first order), forest type (mixed), parent rock (igneous), and soil depth (<46 cm). The relationship between landslide soil volume and environmental factors showed positive correlation. The variables of vertical slope (complex), altitude (<1,201 m), and soil depth (<46 cm) correlated significantly at 1 % level.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2007.11a
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• pp.97-98
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• 2007

• Proceedings of the KIEE Conference
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• 2001.07b
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• pp.871-873
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• 2001

The switched reluctance motor (SRM) is gaining much interest as a candidate for electric vehicle for its simple and rugged construction, ability of extremely high-speed operation. This paper is to design and investigate the capabilities of the SRM for low speed electric vehicle (LSEV).