• 수산해양기술연구
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• 제47권4호
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• pp.300-315
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• 2011

The investigation for the catch and species composition in the deep sea of the East Sea was done to search marine organisms using some kinds of traps with small commercial fishing vessel near Goseong, Korea from 2008 to 2009. The experiments was carried out with some different traps at the depth of 200m, 400m, 700m and 1,000m. In 2008, the amount of catch with rectangular, cone and drum net traps was 91.4kg with twelve trips and the proportion of catch with rectangular and cone traps was high. Dominant species caught were Aniwan whelk (57%), snow crab (12%), hunchback sculpin (7%) and red snow crab (5%). The catch of Aniwan whelk was done well with rectangular and cone traps at the depth of 700-1,000m. Snow crab was mainly caught with rectangular and cone traps at the shallower depth of 200m, while red snow crab caught a lot with cone trap at the deeper depth of 700m. In 2009, the amount of catch with rectangular, cone and round traps was 92.4kg with nine trips. The proportion of catch with round trap was higher than that with cone trap. Dominant species caught were snow crab (67.1%), Aniwan whelk (14.8%) and northern shrimp (9.8%). Snow crab was caught well with round trap at the depth less than 400m.

• 수산해양기술연구
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• 제50권3호
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• pp.262-273
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• 2014

To investigate the catching efficiency of a biodegradable round trap for a swimming crab (Portunus trituberculatus), four types of material (two types of biodegradable net, one type of biodegradable plus polyethylene net and one type of polyethylene net) for traps were used in the field test, and the field tests were carried out 11 times in the Boryeong fishing ground of Korea, 2012~2013. The catching efficiency of four type traps was analyzed by catch per trap, weight per trap and mean weight per individual of small and large size swimming crab. Statistical Kruskal-Wallis test was carried out to verify the significant difference for the efficiency between types of material. The catch in number of swimming crab was 9,015 and formed about 94.3% of total catch. In the results of catching efficiency of traps, there was a little difference in catch per trap, weight per trap and mean weight per individual of swimming crab in-situ data. But there was no significant difference in catching efficiency between the biodegradable traps and the PE trap (P>0.05), so it's needed to use widely a biodegradable trap for the conservation and sustainable management of swimming crab resources.

• 수산해양기술연구
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• 제39권3호
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• pp.174-180
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• 2003

서해안의 연안성 어종인 쥐노래미는 백령, 대청 근해에서 주요 어종의 하나로 주로 통발에 의해 어획되고 있다. 쥐노래미 어업이 지속적으로 유지되기 위해서, 소형어를 보호하는 효율적인 어구 사용과 현재 정해져 있는 망목크기가 적정한가를 파악하기 위하여 망목선택성 실험을 하였다. 쥐노래미 통발에 대한 망목선택성을 Kitahara의 방법에서 객관성이 있도록 선택성 곡선을 다항식이나 로지스틱 곡선식을 이용하여 구하고, 쥐노래미의 최소성숙체장에 대한 적정망목크기를 구하였다. 통발의 경우, 선택성 곡선(마스터 곡선)을 구할 때 적용한 다항식은 삼중자망에서와는 달리 어체가 큰 부분에서 보정치가 지나치게 작용하여 선택성 곡선을 신뢰할 수 없었고, 로지스틱 곡선이 보다 합리적이었으며, 그 식은 다음과 같았다. s(R) =(equation omitted) 쥐노래미의 최소성숙체장은 21.4㎝로 알려져 있으며, 이 최소성숙체장에 대해 50% 어획될 확률 (L/sub 50/)이 되는 망목크기는 37.0㎜이었다. 현재 통발의 망목크기 제한은 35㎜로 규정되어 있는데 쥐노래미의 경우에 다소 작으나 큰 차이는 없는 것 같다.

• 한국전기전자재료학회논문지
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• 제22권2호
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• pp.101-106
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• 2009

In this paper, Thin films of $HfO_2$/Hf were deposited on p-type wafer by Atomic Layer Deposition (ALD). We studied the electrical and material characteristics of $HfO_2$/Hf/Si MOS capacitor depending on thickness of Hf metal layer. $HfO_2$ films were deposited using TEMAH and $O_3$ at $350^{\circ}C$. Samples were then annealed using furnace heating to $500^{\circ}C$. Round-type MOS capacitors have been fabricated on Si substrates with $2000\;{\AA}$-thick Pt top electrodes. The composition rate of the dielectric material was analyzed using TEM (Transmission Electron Microscopy), XRD (X-ray Diffraction) and XPS (X-ray Photoelectron Spectroscopy). Also the capacitance-voltage (C-V), conductance-voltage (G-V), and current-voltage (I-V) characteristics were measured. We calculated the density of oxide trap charges and interface trap charges in our MOS device. At the interface between $HfO_2$ and Si, both Hf-Si and Hf-Si-O bonds were observed, instead of Si-O bond. The sandwiched Hf metal layer suppressed the growing of $SiO_x$ layer so that $HfSi_xO_y$ layer was achieved. And finally, the generation of both oxide trap charge and interface trap charge in $HfO_2$ film was reduced effectively by using Hf metal layer.

• 수산해양기술연구
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• 제39권1호
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• pp.50-55
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• 2003

자원관리형 자망$.$통발 어구어법 기술개발을 위한 연구의 일환으로 서해구의 자망$.$통발어업에 대한 현황을 파악하고, 서해구의 대표적인 어종 중의 하나인 민어를 어획대상으로 하는 민어 연안 유자망어업과 피뿔고둥을 주 어획대상으로 하는 기타 통발어업을 연구대상으로 선정하여 현장조사 등을 통하여 조업실태와 대상어종의 체장조성 등을 조사한 결과는 다음과 같다. 1. 서해구의 연안어업 총 허가건수는 12,944건(조업건수 7,558건; 2001년 12월말 현재)이며, 그 중에서 자망어업이 5,154건(조업건수 3,724건)으로 39.8%, 통발어업이 1,025건(조업건수 662건)으로 7.6%를 차지하였으며, 서해구에서의 조업율은 연안 자망어업과 연안 통발어업이 각각 72.3%와 64.6%로서 타 업종에 비해 비교적 높은 편이었다. 2. 민어 유자망어업의 주어기는 7월 중순부터 9월 초순까지이며, 주어장은 서해 남부 연안의 섬주변의 수심 약 15∼50m(주로 30m 이내)이고, 조업은 저질이 펄 또는 사니질인 해역에서 사리때를 전후하여 1일 1∼2회 1주일 정도 창조시 투망하여 1∼2시간 정도 어구가 조류에 표류하도록 한 후 양망하는 것으로 조사되었다. 3. 민어 유자망어업의 현장조사 결과 어획된 139마리의 체장 범위는 43.0∼120cm이고, 모우드(mode)는 85cm였으며, 꽃게$.$병어$.$가오리 등이 소량 혼획되었다. 4. 서해구에서의 피뿔고둥을 주대상으로 하는 기타 통발어업은 꽃게 어기(9월 초순∼10월 중순)를 제외한 시기에 연중 조업하며, 서해 연안의 수심이 10∼20m이고 저질이 사니질인 해역에서 보통 전날 투망하여 다음 날 양망하는 것으로 조사되었다. 5. 기타 통발어업의 현장조사 결과 조사기간 중 어획된 피뿔고둥 5,372마리의 갑장 범위는 4.5∼8.5cm이고, 모우드(mode)는 7.5cm였으며, 피뿔고등 91.7%, 배꼽고등 4.8%, 민꽃게$.$쥐노래미$.$조피볼락 등이 3.5% 정도 혼획되었다.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2003년도 학술대회
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• pp.81-85
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• 2003

In round numbers the 100 strains of entomopathogenic nematodes were isolated through the investigation of cultivated including the ginseng forming cultivated and forest soil samples by silkworm trap. The 28 strains of nematodes were selected among the isolated entomopathogenic nematodes that were confirmed the pathogenicity against Holotrichia morosa, Hoiotrichia diomphalia and Ectinus sericeus, the pest of korean ginseng and silkworm. Pathogenicity of the 2025, 2027, 2028, 2034, 2039 and 2057 strains was excellent. Selected entomopathogenic nematodes are classified of two species by morphologiacl and molecular studies, which were Sterinernma carpocapsae sp. and Diplogaster lethier sp.. Diagnostic characters include body length, lateral field pattern, tail shape and so on. The DNA sequences of the ITS region of rDNA shows similar to S. carpocapsae and .D. lethier. Isolated entomopathogenic nematodes were demonstrates that are quite within the realms of possibility for biological control agents of the pests of Korean Ginseng.

• 농약과학회지
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• 제4권2호
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• pp.50-55
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• 2000

토마토를 가해하는 아메리카잎굴파리의 발생과 가해양상 그리고 살충제처리에 의한 아메리카잎굴파리의 방제가 토마토 생육에 미치는 영향을 구명하기 위하여 1997년부터 1998년까지 전남지방의 플라스틱하우스를 중심으로 연구하였다. 봄 재배에서 황색끈끈이트랩과 sweeping에 의한 조사 결과는 5월 하순부터 밀도가 급격히 증가하였으며 sweeping 보다는 끈끈이트랩 조사에 의한 밀도변동이 뚜렷하였다. 피해엽율은 5년 이상 계속하여 토마토를 재배한 포장에서는 년중 피해엽을 확인할 수 있었으며, 4월 상순 이전에는 20%이하, 중순 30%, 하순 40%, 5월 상순에 60%, 하순이후에는 80%수준이었다. 반면 처녀재배지에서는 정식후 6주째부터 피해엽이 나타나기 시작하였으며 피해엽율은 80%수준으로 급격히 높아졌다. 가을재배에서는 재배연수에 관계없이 정식때부터 피해엽율이 80%수준을 유지하였다. 성충과 유충의 발생소장은 봄재배의 경우 5월하순부터 성충이 유인되었고 6월하순에 포회량이 가장 많았으나 종령유충은 발생초기부터 주당 $6{\sim}8$마리로 수확기까지 거의 비슷한 밀도 수준을 유지하였다. 가을재배의 경우 정식직후부터 3주째까지는 성충과 유충의 밀도가 높게 유지되었으나 4주째인 9월상순부터는 밀도가 급격히 떨어졌다. 살충제처리에 의한 방제 효과는 클로르페나피르 유제, 싸이로마진 수화제, 아바멕틴 유제가 아메리카잎굴파리의 유충의 밀도를 효과적으로 억제시킬 수 있었다.

• Animal Bioscience
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• 제34권10호
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• 한국수산과학회지
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• 제47권5호
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• pp.637-644
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• 2014

Two Chionoecetes crabs, C. opilio and C. japonicus are primary targets of trap fisheries in the East Sea. To promote the sustainable management of these species, we investigated their reproductive biology and spatial distribution based on samples collected in standardized traps in the coastal waters of eastern Korea from September 2007 to August 2008. The estimated spawning season of C. opilio was March-April, whereas that of C. japonicus continued year-round. The estimated molting period of both species was September-October. Fecundity of C. opilio was proportional to female, size, ranging from 44,791 to 151,538 eggs, and the estimated body size at 50% sexual maturity was 63.9 mm carapace width. Egg bearing female C. opilio were mostly collected depths of 200-300 m, particularly off Hupo and Chuksan, suggesting that these areas are their major spawning grounds. In contrast, female C. japonicus were mostly collected at depths of 400-600 m, peaking at 600 m. The species-specific catch rate was higher for C. opilio above 400 m, similar at ca. 450 m, and higher for C. japonicus below 500 m. These vertical differences indicate apparent spatial segregation of the species, suggesting that spatially-explicit fisheries management plans may be necessary for mitigating conflicts between the respective crab fisheries and maintaining these crabs.

• Current Optics and Photonics
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• 제1권4호
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• pp.412-420
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• 2017

Multinucleated bone resorptive osteoclasts differentiate from bone marrow-derived monocyte/macrophage precursor cells. During osteoclast differentiation, mononuclear pre-osteoclasts change their morphology and biochemical characteristics. In this study, Raman spectroscopy with multivariate techniques such as Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to extract biochemical information related to various cellular events during osteoclastogenesis. This technique allowed for label-free and noninvasive monitoring of differentiating cells, and clearly discriminated four different time points during osteoclast differentiation. The Raman band intensity showed significant time-dependent changes that increased up to day 4. The results of Raman spectroscopy agreed with results from atomic force microscopy (AFM) and tartrate-resistant acid phosphatase (TRAP) staining, a conventional biological assay. Under AFM, normal spindle-like mononuclear pre-osteoclasts became round and smaller at day 2 after treatment with a receptor activator of nuclear $factor-{\kappa}B$ ligand and they formed multinucleated giant cells at day 4. Thus, Raman spectroscopy, in combination with PCA-LDA, may be useful for noninvasive label-free quality assessment of cell status during osteoclast differentiation, enabling more efficient optimization of the bioprocesses.