• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.4
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• pp.300-315
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• 2011

The investigation for the catch and species composition in the deep sea of the East Sea was done to search marine organisms using some kinds of traps with small commercial fishing vessel near Goseong, Korea from 2008 to 2009. The experiments was carried out with some different traps at the depth of 200m, 400m, 700m and 1,000m. In 2008, the amount of catch with rectangular, cone and drum net traps was 91.4kg with twelve trips and the proportion of catch with rectangular and cone traps was high. Dominant species caught were Aniwan whelk (57%), snow crab (12%), hunchback sculpin (7%) and red snow crab (5%). The catch of Aniwan whelk was done well with rectangular and cone traps at the depth of 700-1,000m. Snow crab was mainly caught with rectangular and cone traps at the shallower depth of 200m, while red snow crab caught a lot with cone trap at the deeper depth of 700m. In 2009, the amount of catch with rectangular, cone and round traps was 92.4kg with nine trips. The proportion of catch with round trap was higher than that with cone trap. Dominant species caught were snow crab (67.1%), Aniwan whelk (14.8%) and northern shrimp (9.8%). Snow crab was caught well with round trap at the depth less than 400m.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.3
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• pp.262-273
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• 2014

To investigate the catching efficiency of a biodegradable round trap for a swimming crab (Portunus trituberculatus), four types of material (two types of biodegradable net, one type of biodegradable plus polyethylene net and one type of polyethylene net) for traps were used in the field test, and the field tests were carried out 11 times in the Boryeong fishing ground of Korea, 2012~2013. The catching efficiency of four type traps was analyzed by catch per trap, weight per trap and mean weight per individual of small and large size swimming crab. Statistical Kruskal-Wallis test was carried out to verify the significant difference for the efficiency between types of material. The catch in number of swimming crab was 9,015 and formed about 94.3% of total catch. In the results of catching efficiency of traps, there was a little difference in catch per trap, weight per trap and mean weight per individual of swimming crab in-situ data. But there was no significant difference in catching efficiency between the biodegradable traps and the PE trap (P>0.05), so it's needed to use widely a biodegradable trap for the conservation and sustainable management of swimming crab resources.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.3
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• pp.174-180
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• 2003

This study investigated the size selectivity of the round traps for greenling (Hexagrammos otakii) in the western sea of Korea. The selection curve for the greenling from the experiments on Oct. 2000 and Ar. 2001 was fitted by Kitahara's method to a polynomial equation and two parameter logistic selection curve. The selectio curve of the latter was more reasonable than that of the former. The equation of selectivity curve obtained using a logistic function with least square method was , s(R)=1/1+exp(-1.1169R+6.4565), where R=1/m, and 1 and m are total length and mesh size, respectively. The size selectivity curve showed that the current regulated mesh size(35mm) in case of the round trap was close to the L50 (37.0mm) of the selection curve for the biological minimum length (21.4cm) of the greenling.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.22 no.2
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• pp.101-106
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• 2009

In this paper, Thin films of $HfO_2$/Hf were deposited on p-type wafer by Atomic Layer Deposition (ALD). We studied the electrical and material characteristics of $HfO_2$/Hf/Si MOS capacitor depending on thickness of Hf metal layer. $HfO_2$ films were deposited using TEMAH and $O_3$ at $350^{\circ}C$. Samples were then annealed using furnace heating to $500^{\circ}C$. Round-type MOS capacitors have been fabricated on Si substrates with $2000\;{\AA}$-thick Pt top electrodes. The composition rate of the dielectric material was analyzed using TEM (Transmission Electron Microscopy), XRD (X-ray Diffraction) and XPS (X-ray Photoelectron Spectroscopy). Also the capacitance-voltage (C-V), conductance-voltage (G-V), and current-voltage (I-V) characteristics were measured. We calculated the density of oxide trap charges and interface trap charges in our MOS device. At the interface between $HfO_2$ and Si, both Hf-Si and Hf-Si-O bonds were observed, instead of Si-O bond. The sandwiched Hf metal layer suppressed the growing of $SiO_x$ layer so that $HfSi_xO_y$ layer was achieved. And finally, the generation of both oxide trap charge and interface trap charge in $HfO_2$ film was reduced effectively by using Hf metal layer.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.1
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• pp.50-55
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• 2003

In order to study on the improvement of gill nets and trap nets fishing for the management of fisheries resource in the coastal area of Yellow Sea, we have investigated the general present condition of those fishing, and the actual fishing operation of gill nets for croaker and trap nets for rock shell which is called the the other trap nets, and then measured the body length of croaker and blue shell caught by their fishing vessels. The results are as follows ; 1. The total number of permission for coastal fishing are 12,944 cases, but the number of operation for coastal fishing are 7,558 cases in the coastal area of Yellow Sea. Among the total number of permission, the gill nets fishing are 5,154 cases with 39.8% but even so the number of operation are 3,724 cases, the trap nets fishing are 1,025 cases with 7.6% but even so the number of operation are 662 cases. On the fishing ratio, the gill nets and trap nets fishing are comparatively higher than the other fishing with 72.3% and 64.6%, respectively. 2. The main fishing period of gill nets for croaker is from the middle of July to early of September, and the main fishing grounds are sandymud bottom of 15-50m in depth around the islands of southern parts of western coastal area, and the fishing operation carry out 1∼2 times per day in flood tide and nets hauling conduct in 1∼2 hours after drifting with current. 3. The distribution range of body length of 139 croakers, which are caught in the gill nets, are 43.0∼120.0㎝ and the mode is 85.0㎝. 4. The main fishing period of trap nets for rock shell which is called the other trap nets is all the year round except the catching period of blue crab from early of September to the middle of October, and the main fishing ground are the sandymud bottom of 10∼20m in depth, and nets hauling conduct in next day after nets casting. 5. The distribution range of maximum carapace of 5,372 rock shells are 4.5∼8.5㎝ and the mode is 7.5㎝.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2003.11a
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• pp.81-85
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• 2003

In round numbers the 100 strains of entomopathogenic nematodes were isolated through the investigation of cultivated including the ginseng forming cultivated and forest soil samples by silkworm trap. The 28 strains of nematodes were selected among the isolated entomopathogenic nematodes that were confirmed the pathogenicity against Holotrichia morosa, Hoiotrichia diomphalia and Ectinus sericeus, the pest of korean ginseng and silkworm. Pathogenicity of the 2025, 2027, 2028, 2034, 2039 and 2057 strains was excellent. Selected entomopathogenic nematodes are classified of two species by morphologiacl and molecular studies, which were Sterinernma carpocapsae sp. and Diplogaster lethier sp.. Diagnostic characters include body length, lateral field pattern, tail shape and so on. The DNA sequences of the ITS region of rDNA shows similar to S. carpocapsae and .D. lethier. Isolated entomopathogenic nematodes were demonstrates that are quite within the realms of possibility for biological control agents of the pests of Korean Ginseng.

• The Korean Journal of Pesticide Science
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• v.4 no.2
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• pp.50-55
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• 2000

Occurrence, Injury aspects and control effect of some chemicals were investigated on tomato in plastic house from 1997 to 1998. Adults trapped by yellow sticky trap and sweeping net were increased from late May abruptly. Survey of population densities by yellow sticky trap was more clear than sweeping net. Damaged leaves were found out all the year round in continuous cropping fields and rate of damaged leaves was below 20% in early April and increased gradually up to 80% level after late May. Otherwise, damaged leaves were appeared from 6 weeks after transplanting in first growing field and then damaged leaves was 80% level in spring culture. In autumn culture, rate of damaged leaves was maintained 80% level from transplanting to harvesting date regardless of cultivation years. Number of adults was trapped from late May and peak was late June but mature larva was $6{\sim}8$ individuals in spring culture. In autumn culture, adult and larval densities was maintained high for 3 weeks after transplanting but begun to decrease from 4 weeks(early September). Chlorfenapyr EC, cyromazine WP and abamectin EC could suppress larval populations of Liriomyza trifolii effectively.

• Animal Bioscience
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• v.34 no.10
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.637-644
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• 2014

Two Chionoecetes crabs, C. opilio and C. japonicus are primary targets of trap fisheries in the East Sea. To promote the sustainable management of these species, we investigated their reproductive biology and spatial distribution based on samples collected in standardized traps in the coastal waters of eastern Korea from September 2007 to August 2008. The estimated spawning season of C. opilio was March-April, whereas that of C. japonicus continued year-round. The estimated molting period of both species was September-October. Fecundity of C. opilio was proportional to female, size, ranging from 44,791 to 151,538 eggs, and the estimated body size at 50% sexual maturity was 63.9 mm carapace width. Egg bearing female C. opilio were mostly collected depths of 200-300 m, particularly off Hupo and Chuksan, suggesting that these areas are their major spawning grounds. In contrast, female C. japonicus were mostly collected at depths of 400-600 m, peaking at 600 m. The species-specific catch rate was higher for C. opilio above 400 m, similar at ca. 450 m, and higher for C. japonicus below 500 m. These vertical differences indicate apparent spatial segregation of the species, suggesting that spatially-explicit fisheries management plans may be necessary for mitigating conflicts between the respective crab fisheries and maintaining these crabs.

• Current Optics and Photonics
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• v.1 no.4
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• pp.412-420
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• 2017

Multinucleated bone resorptive osteoclasts differentiate from bone marrow-derived monocyte/macrophage precursor cells. During osteoclast differentiation, mononuclear pre-osteoclasts change their morphology and biochemical characteristics. In this study, Raman spectroscopy with multivariate techniques such as Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to extract biochemical information related to various cellular events during osteoclastogenesis. This technique allowed for label-free and noninvasive monitoring of differentiating cells, and clearly discriminated four different time points during osteoclast differentiation. The Raman band intensity showed significant time-dependent changes that increased up to day 4. The results of Raman spectroscopy agreed with results from atomic force microscopy (AFM) and tartrate-resistant acid phosphatase (TRAP) staining, a conventional biological assay. Under AFM, normal spindle-like mononuclear pre-osteoclasts became round and smaller at day 2 after treatment with a receptor activator of nuclear $factor-{\kappa}B$ ligand and they formed multinucleated giant cells at day 4. Thus, Raman spectroscopy, in combination with PCA-LDA, may be useful for noninvasive label-free quality assessment of cell status during osteoclast differentiation, enabling more efficient optimization of the bioprocesses.