• Title/Summary/Keyword: rough surfaces

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Gluconacetobacter persimmonis sp. nov., Isolated from Korean Traditional Persimmon Vinegar

  • Yeo, Soo-Hwan;Lee, Oh-Seuk;Lee, In-Seon;Kim, Hyun-Soo;Yu, Tae-Shick;Jeong, Yong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.276-283
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    • 2004
  • Screening was performed to isolate cellulose-producing microorganisms from the Korean traditional fermented persimmon vinegar. The resulting strain, KJ $145^{T}$, was then taxonomically investigated by phenotypic characterization, particularly chemotaxonomic, and by phylogenetic inference based on a 16S rDNA sequence analysis including other related taxa. Strain KJ $145^{T}$ was found to grow rapidly and form pale white colonies with smooth to rough surfaces on a GYC agar. Strain KJ $145^T$ also produced acetate from ethanol, and was tolerable to 10% ethanol in SM medium. In a static culture, a thick cellulose pellicle was produced, and in GYC broth, the strain grew at temperatures ranging from 28 to $40^\circ{C}$ with an optimum pH of 4.0. The genomic DNA G+C content of strain KJ $145^T$ was 61.9 mol%, and the predominant ubiquinone was Q 10 as the major quinone and Q9 as the minor quinone. The major cellular fatty acids were $C_{16:0}$ and the sum in feature 7 ($C_{18:1}$ w9c, w12t and/or w7c). A 16S rRNA-targeted oligonucleotide probe specific for strain KJ $145^T$was constructed, and the phylogenetic position of the new species was derived from a 16S rDNA-based tree. When comparing the 16S rDNA nucleotide sequences, strain KJ $145^T$ was found to be most closely related to G. hansenii LMG $1527^T$ (99.2%), although KJ $145^T$ was still distinct from G. hansenii LMG $l527^T$ and G. xylinus LMG $1515^T$ in certain phenotypic characteristics. Therefore, on the basis of 16S rDNA sequences and taxonomic characteristics, it is proposed that strain KJ $145^T$ should be placed in the genus Gluconacetobacter as a new species, Gluconacetobacter persimmonis sp. nov., under the type-strain KJ $145^T$ (=KCTC =$10175BP^T$=KCCM=$10354^T$).

Replication and Sequential Development of Adherent Mycoplasma Pneumoniae Studied by Light and Electron Microscopies (광학(光學) 및 전자현미경기술(電子顯微鏡技術)에 의(依)한 Mycoplasma pneumoniae의 분열(分裂)과 연속분화(連續分化)에 관(關)한 연구(硏究))

  • Kim, C.K.;Pfister, Robert M.
    • Applied Microscopy
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    • v.12 no.2
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    • pp.11-22
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    • 1982
  • The morphological development of Mycoplasma pneumoniae attached to solid surfaces was examined by light and electron microscopies. Critical point drying and carbon replication techniques revealed that during the growth cycle of developing microcolonies, the morphological form coincided with the pH of the culture. M. pneumoniae appeared to have a well defined morphology associated with age of the culture. The organisms were dimorphic, with round cells capable of reproduction and segments consisting of a spindle shaped body with one pointed and one knob-like end. Starting with single cells, there were the following stages in the development of a culture: replication stage through binary fission and segmentation, stage of confluency, and a degeneration stage into rough spherical forms. The round cells appearrd to replicate by binary fission during the lag and early log phases of growth, while spindle segments replicated by segmentation during most of the logarithmic growth. The growth of the filaments and replication of the segments occured at the knob-like ends, showing a type of polarity, and formed a meshwork across the surface. This development could be cycled under favorable growth conditions, but the culture aged and when the conditions became adverse(e.g. pH 6.8 or lower), filamentous cells converted to spherical forms, losing their reproductive capability.

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Rheological and Failure Properties of Polycarbonate/Poly(butylene terephthalate) Blends (폴리카보네이트/폴리(부틸렌 테레프탈레이트) 블렌드의 유변학적 및 파괴특성)

  • Nah, Chang-Woon;Huh, Mong-Young;Choi, Dae-Hwan;Kook, Jeong-Ho;Hwang, In-Ra;Jeong, Kwang-Un;Hong, Chang-Kook
    • Polymer(Korea)
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    • v.31 no.5
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    • pp.399-403
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    • 2007
  • Trans-esterification behavior of polycarbonate/poly(butylene terephthalate) (PC/PBT) blends was investigated during the melt mixing process. Rheological and fracture behaviors, and fracture morphology were also investigated as a function of PC/PBT blend ratio. Based on FT-IR and $^1H-NMR$ results, a trans-esterification reaction was confirmed to occur between PC and PBT during the melt mixing process. The melt index(MI) decreased with increased PC content, indicating the higher flow resistance of PC. The storage and loss moduli were increased by increasing the PC loading, and the PC/PBT blends were rheologically incompatible based on the Cole-Cole plot. The tensile property increased linearly with the increased PC content. However, the impact strength increased until 50 wt% of PC loading, notably around $30{\sim}40wt%$, and then was levelled off at 50 wt%. Rough ridges were formed on the impact fracture surfaces above the 40 wt% of PC content, supporting the observed higher impact strength in this range.

The effect of Zirconium Nitride coating on shear bond strength with denture base resin in Co-Cr alloy and titanium alloy (질화 지르코늄 코팅이 코발트 크롬 합금과 타이타늄 합금에서 의치상 레진과의 전단결합강도에 미치는 영향)

  • Park, Chan;Lee, Kyoung-Hun;Lim, Hyun-Pil
    • Journal of Dental Rehabilitation and Applied Science
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    • v.32 no.3
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    • pp.194-201
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    • 2016
  • Purpose: The purpose of this study was to evaluate of Zirconium Nitride (ZrN) coating on shear bond strength with denture base resin in Co-Cr and Ti-6Al-4V alloy. Materials and Methods: Co-Cr and Ti-6Al-4V alloy disks (10 mm in diameter, 2.5 mm in thickness; each other: n = 14) were prepared and divided with 2 groups each other by ZrN coating. After primer was applied to disks surface, denture base resin with diameter 6 mm, height 5 mm was bonded on metal disk surface. After surface roughness was measured by Profiler, shear bond strength was determined with Universal testing machine and analyzed with two-way ANOVA. The specimen surfaces and failure mode were examined using a scanning electron microscope. Results: ZrN coated groups showed significantly higher rough surface than non-coated groups (P < 0.05). Irrespective of alloy materials, shear bond strength of ZrN coated groups were lower than non-coated groups (P < 0.001). The scanning electron microscope (SEM) of ZrN coated groups showed mixed and adhesive fractures. Conclusion: ZrN coating weakened bonding strength between denture base resin and Co-Cr, Ti-6Al-4V alloy.

RETROSPECTIVE STUDY OF SHORT ROUGH SURFACE IMPLANTS (짧은 길이의 거친 표면 임프란트에 대한 후향적 연구)

  • Kong, Jun-Ha;Lee, Baek-Soo;Kim, Yeo-Gab;Kwon, Yong-Dae;Yoon, Byung-Wook;Choi, Byung-Joon
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.1
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    • pp.41-45
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    • 2009
  • Background: Reduced bone height is one of the major problems faced in restoring tooth loss with implants. By the use of short implants, it is possible to reduce complicated and invasive treatment such as bone graft, allowing more simple surgery. But short implants are generally considered to have lower success rates than that of standard implants. Purpose: To assess the results of short Straumann implants by a retrospective study of short Straumann implants with TPS(titanium plasma-sprayed) and SLA(sandblasted, large grit, acid etched) surfaces. Materials and methods: 173 implants in 106 patients who received short Straumann implant surgery(${\le}8\;mm$) in the department of oral and maxillofacial surgery, Kyunghee Dental Hospital, from February 1996 to October 2006 were selected and studied. All of the implants were followed up after prosthetic rehabilitation. The average follow-up period was 34 months with 119 months as the longest follow up period. The average follow-up period after prosthetic rehabilitation was 31 months. 64 females(60.4%) and 42 males(39.6%) participated in the research with the age range of 19 to 85(mean age 47). 20 patients(18.9%) were under 40, 85 patients(80.2%) were over 40 and under 70, and only one patient(0.9%) was over 70 years old. Results: 27 implants(15.6%) had TPS surface while 146 implants(84.4%) had SLA surface. 9 implants(5.2%) were 3.3, 108 implans(62.4%) were 4.1mm and 56 implants(32.4%) were 4.8mm in diameter. 167 implants(96.5%) were 8mm and 6 implants(3.5%) were 6mm in length. There were 24 implants(13.9%) on the maxillas and 149 implants(68.8%) on the mandibles. 119 implants(68.8%) were rehabilitated with FPD(fixed partial denture), 47 implants(27.2%) with single crowns and 4 implants(2.3%) with overdentures. Among the fixed partial dentures, 30 of them were splinted with short implants only. After over an year of follow-up period, 139 implants(96.5%) out of 144 implants showed marginal bone loss of less than 1mm. 3 out of 173 implants failed showing 98.27% survival rate. Conclusions: The use of short Straumann implants(${\le}8mm$) can be a simple and reliable treatment method in minimal residual bone height.

THE EFFECT OF ACID ETCHING ON GLASS IONOMER CEMENT SURFACES (Glass ionomer cement 표면의 산부식 효과에 관한 연구)

  • Han, Seung-Weon;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.18 no.1
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    • pp.1-26
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    • 1993
  • The purpose of this study was to investigate the effect of acid etching on the surface appearance and fracture toughness of five glass ionomer cements. Five kinds of commercially available glass ionomer cements including chemical curing filling type, chemical curing lining type, chemical curing metal reinforced type, light curing tilling type and light curing lining type were used for this study. The specimens for SEM study were fabricated by treating each glass ionomer cement with either visible light curing or self curing after being inserted into a rubber mold (diameter 4mm, depth 1mm). Some of the specimens were etched with 37% phosphoric acid for 0, 15, 30, 60, go seconds, at 5 minutes, 1 hour and 1 day after mixing of powder and liquid. Unetched ones comprised the control group and the others were the experimental groups. The surface texture was examined by using scanning electron microscope at 20 kV. (S-2300, Hitachi Co., Japan). The specimens for fracture toughness were fabricated by curing of each glass ionomer cement previously inserted into a metal mold for the single edge notch specimen according to the ASTME399. They were subjected to a three-point bend test after etching for 0, 30, 60, and 90 seconds at 5 minutes-, 1 hour-and 1 day-lapse after the fabrication of the specimens. The plane strain fracture toughness ($K_{IC}$) was determined by three-point bend test which was conducted with cross-head speed of 0.5 mm/min using Instron universal testing machine (Model No. 1122) following seven days storage of the etched specimens under $37^{\circ}C$, 100% humidity condition. Following conclusions were drawn. 1. In unetched control group, crack was present, but the surface was generally smooth. 2. Deterioration of the surface appearance such as serious dissolving of gel matrix and loss of glass particles occured as the etching time was increased beyond 15 s following Immediate etching of chemical curing type of glass ionomer cements. 3. Etching after 1 h, and 1 d reduced surface damage, 15 s, and 30s etch gave rough surface appearance without loss of glass particle of chemical curing type of glass ionomer cements. 4. Light curing type glass ionomer cement was etched by acid, but there was no difference in surface appearances according to various waiting periods. 5. It was found that the value of plane stram fracture toughness of glass ionomer cements was highest in the light curing filling type as $1.79\;MNm^{-1.5}$ followed by the light curing lining type, chemical curing metal reinforced type, chemical curing filling type and chemical curing lining type. 6. The value of plane stram fracture toughness of the chemical curing lining type glass ionomer cement etched after 5 minutes was lower than those of the cement etched after 1 hour or day or unetched (P < 0.05). 7. Light curing glass ionomer cement showed Irregular fractured surface and chemical curing cement showed smooth fractured surface.

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Formation of amorphous Ga2O3 thin films on Ti metal substrates by MOCVD and characteristics of diodes (MOCVD에 의한 Ti 금속 기판 위의 비정질 Ga2O3 박막 형성과 다이오드 특성)

  • Nam Jun Ahn;Jang Beom An;Hyung Soo Ahn;Kyoung Hwa Kim;Min Yang
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.33 no.4
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    • pp.125-131
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    • 2023
  • Ga2O3 thin films were deposited on Ti substrates using metal organic chemical vapor deposition (MOCVD) at temperatures ranging from 350 to 500℃. Lower deposition temperatures were chosen to minimize thermal deformation of the Ti substrate and its impact on the Ga2O3 film. Film surfaces tended to become rough at temperatures below 500℃ due to three-dimensional growth, but the film formed at 500℃ had the most uniform surface. All deposited films were amorphous in structure. Vertical Schottky diodes were fabricated and I-V and C-V measurements were performed. I-V measurements showed higher operating voltages compared to a typical SBD for films grown at different temperatures. The sample grown at 500℃, which had the most uniform surface, exhibited the lowest operating voltage. Higher growth temperatures resulted in higher capacitance values according to C-V measurements.

Effect of Liquefied Digestive Medicine on the Surface of Composite Resin

  • Kim, Min-Young;Lim, Hee-Jung;Kim, Ha-Eun;Kim, Hyun-Jeong;Yu, Hye-Kang;Choi, Soo-Jin;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.22 no.1
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    • pp.20-29
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    • 2022
  • Background: The purpose of this study is to investigate the effect of liquefied digestive medicines on the composite resin surface. Methods: Three types of liquefied digestive medicines (Gashwalmyeongsu, Wicheongsu, and Saengrokcheon) were selected as experimental groups, Samdasoo and Chamisul as negative controls, and Trevi as positive controls were selected to measure pH and titratable acidity. The samples filled with resin at acrylic were made total 300, 50 per group. To evaluate the erosion risk of the composite resin, the specimens were immersed in a liquefied medicine for 1, 3, 5, 15, and 30 minutes, and then the surface microhardness was measured using the Vickers Hardness Number, and the surface change was observed with scanning electron microscope (SEM). Results: The average pH of the three liquefied medicine was 3.75±0.30, the Saengrokcheon was the lowest at 3.45±0.01, and the Trevi was 4.66 and Samdasoo and Chamisul were 7.40 and 8.58, respectively. The amount of NaOH reaching pH 5.5 and 7.0 was the lowest in the order of Trevi, Gashwalmyeongsu, Wicheongsu, and Saengrokcheon. The largest surface hardness reduction value was shown in Gashwalmyeongsu (-11.85±3.73), followed by Saengrokcheon (-9.79±3.11) and Wicheongsu (-8.28±2.83), and Samdasoo (-0.84±1.56) and Chamisul (-6.24±0.42) had relatively low surface hardness reduction values. However, Trevi (-16.67±5.41), a positive control group containing carbonic acid, showed a higher decrease in surface hardness than the experimental group. As a result of observation with SEM, experimental group and positive control group, showed rough surfaces and irregular cracks, and negative control groups showed smooth patterns similar to before immersion. Conclusion: The liquefied digestive medicine with low pH could weaken the composite resin surface, and the carbonic acid component could more effect on the physical properties of the composite resin than pH.

Experimental Study on the Gas Permeability of Nuclear Power Plant Concrete with Construction Joints (시공이음 생성에 따른 원전 콘크리트의 기체투기성에 대한 실험적 연구)

  • Eun-A Seo;Do-Gyeum Kim;Ho-Jae Lee
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.12 no.3
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    • pp.290-297
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    • 2024
  • In this study, the gas permeability characteristics according to the construction joint were evaluated under pressure conditions ranging from 0 to 300 psi. The pore distribution and pore ratio were analyzed using X-ray CT. The average porosity of the OPC specimen was 0.74 %, while the FA specimen showed a relatively low pore ratio of 0.51 to 0.65 % regardless of the presence of the construction joint. For the OPC and FA plain specimens with a thickness of 50 mm without construction joints, no gas permeation occurred below 300 psi. At 300 psi, the gas permeation rates of the specimens with rough and smooth construction joint surfaces were 0.28 L/min and 0.31 L/min, respectively, which were 1.7 times higher than those of the specimens without construction joints. This is because the construction joints affected the distribution of the pores and coarse aggregates and the penetration path within the concrete. The porosity in the construction joint was the highest at about 2 %, and it was confirmed that the pore ratio gradually increased as it approached the joint.

EFFECT OF VARIOUS MECHANICAL TREATMENTS ON TITANIUM PLASMA SPRAYED IMPLANT SURFACES (Titanium plasma sprayed implant에 관한 여러가지 기계적 표면처리방법이 implant표면조도에 미치는 영향)

  • Yu, Hyeon-Seok;Park, Jae-Wan;Suh, Jo-Young
    • Journal of Periodontal and Implant Science
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    • v.23 no.3
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    • pp.493-506
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    • 1993
  • For maintenance of an ailing or failed implant it is essential to treat the implant fixture surface so as to remove bacterial endotoxin and make a surface tolerated by surrounding soft and hard tissue. Thus in this study the method that makes the smoothest surface treated with a high speed diamond bur, a low speed diamond bur, a stone, a rubber point or Jetpolisher was studied. With the profilomenter a mean value of $R_{max}$ was measured. The hight speed diamond, the rubber point and the Jetpolisher showed a mean $R_{max}\;7.77{\mu}m$. The low speed diamond bur, the rubber, point and the Jetolisher made a mean value of $R_{max}\;8.44{\mu}m$. The stone, the rubber point and the Jetolisher showed the smoothest surfaces with a mean value of $R_{max}\;6.24{\mu}m$. TPS (titanium plasma sprayed) areas showed a mean vlaue of $R_{max}\;24.42{\mu}m$, and the smooth surfaced titanium disc manufactured by the company (IMZ, Germany) shows a mean value of $R_{max}\;3.00{\mu}m$. Under the SEM examination the disc treated with a high speed diamond bur, a rubber point and a Jetpolisher showed partially remaining TPS particles, but the height of these particles were reduced remarkably compared with those of the original TPS. The disc treated with a low speed diamond bur, a rubber point and a Jetpolisher showed a rough topography with remaining TPS on the entire surface. A stone, a rubber point and a Jetpolisher removed almost TPS and the bulk titanium metal was exposed and some scratches were made by the stone. All treated discs were revealed as rougher than the smooth surface disc manufactured by the company. An untreated TPS disc shows a very irregular surface and a $40{\mu}m$ height of the plasma sprayed areas.

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