• 제목/요약/키워드: rotavirus

검색결과 240건 처리시간 0.021초

Prevalence of rotavirus genotypes in South Korea in 1989-2009: implications for a nationwide rotavirus vaccine program

  • Than, Van Thai;Kim, Wonyong
    • Clinical and Experimental Pediatrics
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    • 제56권11호
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    • pp.465-473
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    • 2013
  • The epidemiology of human group A rotavirus was analyzed by examining genotypic data acquired from 1989 to 2009 in South Korea. This information was derived from all the available published articles on rotavirus studies in South Korea, retrieved from both the PubMed and KoreaMed databases. Four common G types (G1, G2, G3, and G4) and three common P types (P[8], P[4], and P[6]) accounted for approximately 93% and 99% of the rotavirus reports, respectively. The G9 type was frequently detected after 2000, and because of this prevalence, it is considered to be the fifth most important G type rotavirus after the G1-G4 genotypes. Less common G types of the virus such as G12, G11, and G10 were detected in some geographic settings, and it is important to consider the context of these subtypes and their epidemiological significance. The P[9] virus genotype was observed in the study and has been discussed in many other studies; however, the P[3], P[10] and P[25] genotypes were rarely detected in the epidemiological research. In general, the distributions of the G and P genotypes showed temporal and geographical fluctuations, and a nationwide rotavirus vaccine program that targeted these genotypes demonstrated effectiveness in protecting against the circulating rotavirus strains. However, further analysis is needed to determine the true long-term effectiveness of these vaccines; the analysis should also consider the unexpected effects of vaccinations, such as vaccineinduced diseases, herd immunity, and changes in host susceptibilities.

Molecular characterization of avian rotavirus isolated in Korea

  • Wang, Jun-Hui;Koo, Bon-Sang;Mo, In-Pil;Kang, Shien-Young
    • 한국동물위생학회지
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    • 제36권1호
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    • pp.23-30
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    • 2013
  • An avian rotavirus (AvRV-2) was isolated from feces of broilers suffering from acute gastroenteritis in 2011. It was the first avian rotavirus isolated in Korea. To investigate the molecular characteristics of AvRV-2, the VP4, VP6, VP7 and NSP4 gene nucleotide sequences were determined and compared with those of rotavirus strains available in the GenBank database. The phylogenetic tree of VP7 gene showed that AvRV-2 had a high degree of nucleotide sequence homology (93.4% to 94.7%) with those of rotaviruses belonging to genotype G19 cluster. The phylogenetic tree of the VP4 gene revealed a high degree of nucleotide sequence homology (95.8% to 95.9%) with genotype P[30] rotaviruses isolated from chickens. The VP6 and NSP4 gene nucleotide sequences showed the highest identities with those of avian strains with 95.3% to 96.4% and 90.3% to 92.2%, respectively. Genetic characterization of the VP4, VP6, VP7 and NSP4 showed that AvRV-2 strain was most closely related to chicken rotavirus strains from Germany and Japan. Comparative nucleotide sequences and phylogenetic analysis indicated that avian rotavirus isolated from broilers belonged to genotype G19P[30] and it was the first report on avian rotavirus infection in Korea.

영아를 위한 어머니의 로타바이러스 예방접종 이행과 관련 요인: 건강신념모형을 중심으로 (Factors Associated with Rotavirus Vaccination Behavior among Mothers of Infants: using the Health Belief Model)

  • 이승현;양숙자
    • 한국보건간호학회지
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    • 제31권1호
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    • pp.34-46
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    • 2017
  • Purpose: This study was conducted to identify the sociodemographic characteristics and health belief related to rotavirus vaccination behavior among mothers of infants. Methods: Data collection was performed through an on-line survey between 13 and 16 October, 2016, and the subjects targeted were 240 mothers with infants between the ages of 8 months and 12 months. The collected data were analyzed using $X^2$ test, Fisher's Exact test, ANOVA, and Logistic Regression with SPSS 23.0 program. Results: The complete rotavirus vaccination rate was 76.7%, the incomplete vaccination rate was 9.2%, and the non-vaccination rate was 14.2%. Factors associated with complete rotavirus vaccination were the mother's age (OR=3.306, 95% CI=1.087 to 10.054), the perceived benefits (OR=6.500, 95% CI=2.163 to 19.528) and the self-efficacy (OR=11.568, 95% CI=2.921 to 45.818), and approximately 40.8% of explanation power was observed. Conclusion: To increase the rotavirus vaccination rate, counseling and education by nurses should be performed to increase the self-efficacy and public awareness of the benefits of rotavirus vaccination for mothers with infants.

음성대조 염색법을 이용한 설사 한우송아지 분변내 바이러스양 입자 검색 (Detection of viral-like particles in feces of Korean indigenous calves with diarrhea by negative staining)

  • 임종수;강춘원;이태욱;김내영;정용운;강문일;한동운;최현성;이채용
    • 한국동물위생학회지
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    • 제22권1호
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    • pp.43-52
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    • 1999
  • Presently, viral isolation in the diarrheal feces can be reached by many tools such as fluorescent antibody test(FA), negative contrast electron microscopy(NCEM), virus neutralization test, cell culture, and so on. The purpose of the study was to aimed at the establishment of simplified NCEM technique which can be efficiently applied for diarrheal feces and also the understanding on prevalence of viral-induced diarrhea in calves. One hundred fourty-seven korean indigenous calves with diarrhea were examined to their feces by the modified NCEM. Among them, 98(66.7%) were confirmed to have one or more viruses in feces. The viruses detected were identified as rotavirus(33.3%), coronavirus(16.3% ), togavirus(10.2%) and herpesvirus(0.7%). Ten cases of combined viral infection were consisted of 8 with rotavirus+coronavirus, one with rotavirus+togavlrus and one with rotavirus+herpesvirus. Dirrheal types could classified by yello-wish watery(44.9a ), blood-tinged(19.7% ), white watery(17.7% ) , brownish watery(14.3%), greenish watery(3.4%) diarrhea, respectively. Yellowish watery diarrhea(66cases) was frequently included rotavirus(31.8%), coronavirus(15.2%), and togavirus(13.6%), respectively. Consequently, these results suggest that the modified NCEM is reliable and efficient diagnostic tool for detection of viruses in the diarrheal feces and many calves rearing in Chonnam province have been exposed to some enteric viral agents mainly including rotavirus and coronavirus.

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국내 양돈장에서 분리한 G9형 돼지로타바이러스의 특성 (Characterization of G9 genotype porcine rotavirus isolated in Korea)

  • 왕쥔후이;이승철;강신영
    • 한국동물위생학회지
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    • 제35권2호
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    • pp.83-90
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    • 2012
  • Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P[23], I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.

제주지역에서 최근 수년간 관찰된 Rotavirus 위장관염의 발생 양상 (The Pattern of Occurence of Rotavirus Gastroenteritis in Jeju Area between 2001 and 2005)

  • 강기수;김재리
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제8권2호
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    • pp.113-116
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    • 2005
  • 목 적: 최근 수년간 국내의 rotavirus 위장관염의 유행시기의 변화에 대한 여러 보고가 있었다. 저자들은 최근 수년간 제주지역에서 Rotavirus 위장관염의 유행 양상이 국내의 다른 지역과 구별되는 특징이 있는지 알아보고자 하였다. 방 법: 2001년 12월부터 2005년 6월까지 제주대학교병원 소아과에 급성 위장관염으로 입원한 환아들 중 로타바이러스 검사상 양성으로 나타난 211명의 환아들을 대상으로 하였다. 로타바이러스 감염의 진단은 대변의 면역크로마토그래피상 양성인 경우로 하였다. 결 과: 로타바이러스 위장관염은 2001년 12월에 13명, 2002년에 32명, 2003년에 79명, 2004년에 48명 이었으며 2005년에는(6월까지) 39명에서 진단되었다. 2002년에서 2004년까지 3년간 계절별 분포를 보면 1월에 40명(25.2%), 2월에 56명(35.2%), 3월에 31명(19.5%)이었으며 4월에 23명(14.5%)이었다. 5월에서 12월까지는 모두 9명(5.6%)에서만 나타났다. 따라서 로타바이러스 위장관염이 1월에서 4월까지 집중적으로(94.4%) 발생하며 나머지 기간동안은 환자발생이 매우 적음을 알 수 있었다. 또한 2002년부터 현재까지 계절적 분포의 뚜렷한 변화는 아직 보이지 않았다. 결 론: 최근 수년간 제주지역에서 로타바이러스 위장관염은 계절에 따른 발생 분포에 큰 차이가 없이 주로 1월부터 4월까지 집중되어 발생하였다.

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효소면역법에 의한 소 로타바이러스 항원 검출 (Detection of bovine rotavirus antigen by enzyme linked immunosorbent assay)

  • 안재문;유기조;이용희;이종인
    • 한국동물위생학회지
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    • 제19권1호
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    • pp.30-38
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    • 1996
  • Enzyme-linked immunosorbent assay (ELISA) was developed to detect rotavirus antigen in fecal samples using VP6-specific monoclonal antibody(2B12). The ELISA for rotavirus antigen detection found to have specificity to all bovine and porcine rotaviruses tested but not to bovine viral diarrhea virus and bovine coronavirus. The ELISA appeared to have similar sensitivity and specificity compared to fluorescence antibody assay(FA) and electropherotyping (PAGE).

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Chicken Egg Yolk Antibodies (IgY) for Prophylaxis and Treatment of Rotavirus Diarrhea in Human and Animal Neonates: A Concise Review

  • Thu, Hlaing Myat;Myat, Theingi Win;Win, Mo Mo;Thant, Kyaw Zin;Rahman, Shofiqur;Umeda, Kouji;Nguyen, Sa Van;Icatlo, Faustino C. Jr.;Higo-Moriguchi, Kyoko;Taniguchi, Koki;Tsuji, Takao;Oguma, Keiji;Kim, Sang Jong;Bae, Hyun Suk;Choi, Hyuk Joon
    • 한국축산식품학회지
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    • 제37권1호
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    • pp.1-9
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    • 2017
  • The rotavirus-induced diarrhea of human and animal neonates is a major public health concern worldwide. Until recently, no effective therapy is available to specifically inactivate the rotavirion particles within the gut. Passive immunotherapy by oral administration of chicken egg yolk antibody (IgY) has emerged of late as a fresh alternative strategy to control infectious diseases of the alimentary tract and has been applied in the treatment of diarrhea due to rotavirus infection. The purpose of this concise review is to evaluate evidence on the properties and performance of anti-rotavirus immunoglobulin Y (IgY) for prevention and treatment of rotavirus diarrhea in human and animal neonates. A survey of relevant anti-rotavirus IgY basic studies and clinical trials among neonatal animals (since 1994-2015) and humans (since 1982-2015) have been reviewed and briefly summarized. Our analysis of a number of rotavirus investigations involving animal and human clinical trials revealed that anti-rotavirus IgY significantly reduced the severity of clinical manifestation of diarrhea among IgY-treated subjects relative to a corresponding control or placebo group. The accumulated information as a whole depicts oral IgY to be a safe and efficacious option for treatment of rotavirus diarrhea in neonates. There is however a clear need for more randomized, placebo controlled and double-blind trials with bigger sample size to further solidify and confirm claims of efficacy and safety in controlling diarrhea caused by rotavirus infection especially among human infants with health issues such as low birth weights or compromised immunity in whom it is most needed.

로타바이러스 백신 접종 유무 및 백신종류에 따른 창자겹침증 발병 연령의 비교: 단일기관 연구 (Difference in the distribution of onset age of intussusception after rotavirus vaccination and according to the type of rotavirus vaccine: single medical center study)

  • 이윤영;이응빈;최광해
    • Journal of Yeungnam Medical Science
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    • 제32권2호
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    • pp.80-84
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    • 2015
  • Background: Rotavirus is the most common cause of severe gastroenteritis in children <5 years of age. The first vaccine, RotaShield was developed, but withdrawn because of its association with increased risk of intussusception. Then, RotaTeq and Rotarix were developed. Although in pre-licensure studies, they were not associated with an increased risk of intussusceptions, in recent studies, it has been controversial. Regarding increased risk of intussusception, we studied the difference in the age of intussusception after rotavirus vaccination. Methods: A retrospective analysis was conducted on 136 patients diagnosed with intussusception at Yeungnam University Medical Center for 4 years in the pre-vaccination period (group A) and in the post vaccination period (group B). Sex, mean age and age distribution of intussusceptions were compared according to the type of rotavirus vaccine (group B-1, RotaTeq; group B-2, Rotarix). Results: The median ages of group A and group B were $18.8{\pm}19.6months$ and $15.5{\pm}10.2months$, with no significant differences (p=0.23). The median ages of group B-1 and group B-2 were $15.3{\pm}9.3months$ and $15.6{\pm}10.8months$, with no significant differences (p=0.91). And No significant difference in the distribution of onset age was observed between groups, and only 6 patients were diagnosed with intussusceptions within 1 month after vaccination. Conclusion: No difference was observed in the distribution of onset age of intussusception after rotavirus vaccination and according to the type of rotavirus vaccine. Our study has a limitation in that it was conducted in part of the Daegu area. Additional study is needed.

하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定) (Isolation and Identification of Porcine Rotavirus from Piglets with Diarrhoea)

  • 김희선;최정옥;김두희;유영수;안수환
    • 대한수의학회지
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    • 제28권1호
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    • pp.89-97
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    • 1988
  • Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.

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