• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.335-340
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• 1997

100% root formation in in vitro cultures was observed regardless of kind and levels of auxin used and explant source. The number of roots/explant was increased in 0.5~1.0 mg/L IAA treatment. Thicker roots were observed with the addition of 9% sucrose compared with medium containing lower sucrose concentrations. Paclobutrazol and chlormequat had no effect on tuberization of formed roots but slightly increased the number of root. In in vivo rooting, soaking of regenerated shoot cuttings to 100 mg/L IBA for 15 to 60 minutes was found effective. Treatment of 0.1% IBA rooting powder and planting in rooting medium composred of vermiculite(1) : perlite(1) gave 100% rooting and survival.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.133-139
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• 1994

Eleutherococcus senticocus MAXIM containing compounds for a cordial and lumbago is a native plant in this country. However it is generally difficult to propagate them by seed. This study was carried out to improve propagation efficacy by cutting method using Eleutherococcus senticosus MAXIM collected Mt. Odae for three years$('90{\sim}'92)$. Rooting was delayed by 3 to 12 days but callus formation and rooting percentage was high by 75%, 30% respectively in the treatment of Rooton-F powder, a chemical for rooting promotion, compared with non-treatment. Rooting percentage and rooting characters were not differenced by treatment for remove rooting inhibition compounds and soil-media mixured by 1:1 of vermiculite and perlite was suitable for elevating rooting ratio. A. chiisanensis and A. sieboldianum were rooted well in any time to be cut, while rooting activity of E. senticosus was different by time to be cut. Rooting ratio of E. senticosus was 26%, 36% and 60% when cutten March 20, July 20 and september 20, respectively.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.31-38
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• 2014

Rooting agents and shading treatments were tested in two different experiments to determine their effects on the establishment success of hardwood cuttings of three highbush blueberry varieties 'Bluecrop', 'Duke' and 'Sunrise'. For the experiment with rooting agents, one-cm long bases of the cuttings were dipped into solutions of IBA or NAA for 5 s, both at 0, 500 and $1000mg{\cdot}L^{-1}$ in 50% ethanol, and were also treated with Rootone$^{(R)}$ powder. Determined 90 days after cutting, the percent rooting and root weight were increased by NAA at $500mg{\cdot}L^{-1}$ in 'Bluecrop' and 'Sunrise', while in 'Duke' IBA at $500mg{\cdot}L^{-1}$ was effective. These auxin treatments were found to work better than a commercial product Rootone$^{(R)}$. The rooting agent-induced increases in root development resulted in better shoot growth of the cuttings in all three varieties, as determined after 90 days of further growth in individual containers. In the experiment with shading treatments, different levels of the shading treatment (30 to 90%) were imposed over the cutting bed under no mist. In all three varieties, 30% shading increased the percent rooting and root and shoot growth, compared to no shading control. However, shading levels higher than 50% shading were found to be inhibitory for hardwood cuttings of highbush blueberries, especially under the environmental conditions with no mist system.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.100-106
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• 2014

In order to develop an efficient in vitro micropropagation technique for a rare plant species, Astragalus membranaceus Bunge var. alpinus N., shoot proliferation and in vitro or in vivo rootings were conducted and hyperhydrated leaf generated from cultures was histologically observed. During shoot induction, no distinct effect on multiple shoot induction was found between BA and kinetin treatment. BA enhanced the number of internodes, whereas kinetin stimulated shoot elongation. Hyperhydrated leaf composed of bigger cells and retarded palisade parenchyma and showed irregular cell arrangement compared to normal leaf. Especially starch content in hyperhydrated leaf was significantly reduced. The best rooting rate was achieved by B5 medium among three different medium (B5, MS and WPM) and 0.1mg/L IBA treatment induced the highest rooting ratio (80%). No statistical difference was induced by explant types (apical bud or axillary bud) in terms of rooting ratio. In vivo cutting induced rooting rate up to 65% by 0.5% IBA/Talc powder treatment. Although in vivo rooting rate was less efficient compared to in vitro rooting, better survival rate was observed after soil acclimatization. Present study suggested that above micropropagation techniques can be used for rapid multiplication as well as in vitro or in vivo conservation of the species.

• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.82-89
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• 2022

In vitro techniques were developed for propagating Vaccinium oldhamii using shoots with apical buds. Explants having an apical bud were cultured on Murashige and Skoog (MS) medium supplemented with 1.0, 2.0, and 5.0 mg/L of each zeatin, thidiazuron, 6-benzylaminopurine (BA), and 6-(γ,γ-dimethylallylamino)purine (2-iP) in order to induce multiple shoots. Among the tested treatments, the 2.0 mg/L of 2-iP proved to be most suited for the multiplication and growth of shoots; the multiple shoot induction rate was 100.0%, the average number of shoots was 7.4 per explant, and the average shoot length was 51.7 mm. The in vitro elongated shoots were rooted on half-strength MS medium containing various concentrations of indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA). However, overall callus overgrowth was observed in all treatments and resulted in necrosis and abnormal shoot growth in root formation. A low concentration (0.5 mg/L) of IBA was appropriate for normal root development and the in vitro rooting rate was 30%. Ex vitro treatments on root formation using various concentrations of IBA with Talc powder and two types of rooting substrates (Flexi-Plugs or Horticultural soil) were examined. The ex vitro rooting rate (80%) and length of roots (32.9 mm) were obtained when the cut ends of the shoots were treated with 1.0 mg/L IBA and cultivated in Horticultural soil for 2 months. These findings suggest that ex vitro rooting is the more effective method for improving root formation in Vaccinium oldhamii than in vitro rooting.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.368-373
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• 2010

Juniperus chinensis var. sargentii Henry is a short and creeping evergreen shrub which reaches about 60 cm in height and only occurs in the northeast Asia and in the top of high mountains over the Korea. The Korea Forest Service protects it strictly by law since J. chinensis var. sargentii is an eccentric plant and possibly may be exterminated soon in Korea. This study was carried out to develop the propagation technique by cutting for conservation of genetic resources of J. chinensis var. sargentii. The rooting responses of branch cuttings, obtained from hard(May) and semi-hard wood shoots (August) to four growth regulators, namely, IAA, IBA, NAA and Rooton(exceptionally powder method) applied at various concentrations(0, 100, 200, 500, 1000 and 2000 $mgL^{-1}$) were examined in mixed soil media. Rooting rate showed significant difference between cutting times, among kinds and among concentrations of growth regulators. The optimum cutting time was April to May in hardwood cutting. The application of IBA 1000 $mgL^{-1}$(rooting rate : 36.4%) was most effective in callus formation and rooting of cutting. Relatively, rooting of cutting of the control taken in May was 30.4%. Root characteristics such as number, length and diameter of root were not significantly affected by kinds and concentrations of growth regulators in hardwood cutting.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.34-35
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• 1988

• Horticultural Science & Technology
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• v.30 no.2
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• pp.129-136
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• 2012

This study was conducted to develop an efficient mass propagation method for the mature $Prunus$ $yedoensis$ Matsumura (43 to 58 years old). Cutting was conducted depending on cutting time, auxin treatments (IBA and NAA treatments mixed with talc powder), and cuttings position on shoots in a plastic house equipped with a fog system without heating. Rooted cuttings were transplanted to a nursery bed, and their growth characteristics were investigated in order to check whether the cuttings are successful or not for roadside tree planting. The average rooting rate was highly significant ($P$ < 0.0001) in all treatments: cutting on June 1st (61.4%) was more than two times greater in rooting rate than that on August 1st (23.6%); IBA 1,000 $mg{\cdot}L^{-1}$ (90.8%) and IBA 500 $mg{\cdot}L^{-1}$ (89.2%) showed much greater rooting rates than those of the other treatments; upper part of the cuttings treated with IBA 1,000 $mg{\cdot}L^{-1}$ showed the highest rooting rate, 96.7%. The interactions among treatments in the average rooting rate were also significant. There were significant differences ($P$ < 0.0001) among the auxin treatments in the survival rate of leafed cuttings transplanted to a nursery bed. The average survival rate was 46.5%, and IBA 1,000 $mg{\cdot}L^{-1}$ treatment was the highest in leafed cuttings 79.2%, but most of leafless cuttings were dead. There were significant differences ($P$ < 0.0001) among the cuttings, grafts, and in the seedlings height, diameter at root collar, the number of roots, branches, and leaves, etc., and the cuttings was the best. We can expect a possibility of mass propagation of improved $P.$ $yedoensis$ Matsumura and a high planting survival rate through the transplanting of cuttings to a nursery bed in which the cuttings should be the following conditions: cutting in June to July, use of the upper part of cuttings, IBA treatment, and rooting in August in a cutting-greenhouse equipped with a fog system.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.3
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• pp.47-53
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• 1999

This study was carried out to suggest the slope revegetation technology of biological engineering using the Ligustrum obtusifolium, which is one of the pioneer plant species. Ahead of the experimental construction, we evaluated the L. obtusifolium's value of biological engineering for the slope stabilization by testing the growth rate after the cuttings were buried for 8 weeks('98. 7. ~ '98. 9.). In this test, it was found that the L. obtusifolium, one of the species deep rooted with developed underground parts, is very effective for the slope stabilization and that the rooting powder(Hormex Powder) gave the better effects on root germination. In April of 1999, the experimental construction of biological engineering technology using recycled L. obtusifolium live cuttings(applied growth-stimulating compound) and green bags was conducted at sandy cut-slope in GLEN ROSS G.C.(Yongin). The slope was tolerant of soil erosion despite of heavy rainfall in the rainy season and many plant species invading for five months('99. 4. ~ '99. 9.). The vegetation research was performed through examining the frequency of each block using the transect method. 21 kinds of plant speices appeared in total area($25.5m^2$) and the dominant species are Digitaria sanguinalis, Setaria viridis, Cyperus amuricus, Persicaria blumei, Artemisia princeps var. orientalis. With regard to life forms, therophytes were shown with a dominant distribution of 66.7% of total species and neophytes relatively with a low distribution of 19.0%. Furthermore, it can be estimated that there is no ecological stabilization of this slope with the result of the ruderal species' occupation of 71.4%. It is too early to argue about ecological mechanical advantages and disadvantages of this technology, but, from the result of this study, it is expected that the slope revegetation technology of biological engineering using L. obtusifolium can be effectively applied to sandy slope(not rock or weathered rock slope) and that the early rapid stabilization and favorable succession could be done with the improvement of soil condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.4
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• pp.287-293
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• 1991

The root-mat formation of infant rice seedlings (8- to 10-day-old) is sometime not enough for machine transplanting because of the too-short nursery period. This experiment was conducted to elucidate the effect of metalaxyl seed-soaking treatment in the mixed solution of metalaxyl, seed disinfectant and insectcides on the root-mat formation of infant rice seedling in machine transplanting. The rice seeds of Odaebyeo were soaked in the mixed solution of metalaxyl, prochloraz and insectcides. with the recommended concentrations for 24 hours at room temperature. Seeding rate was 220g per seed tray (30 x 60 x 3cm). Metalaxyl (25% wettable powder), a fungicide. was used in 1. 000 times dilution as a promoting substance on the root-mat formation. Generally, the metalaxyl-treated seeds markedly increased the root number and length, and rooting activity of infant rice seedlings as compared with the control, thus the root-mat formation was excellent. When the rice seeds were sterilized by the mixed solution of prochloraz and insectcides, metalaxyl could be used together for increasing root-mat rormation of infant rice seedlIngs due to no interaction among agro-chemicals used. Seedling damping-off and physiological seedling rot were also controled in the seedlings of metalaxy-treated seeds. The root-mat of metalxyl-treated seeds had higher hardness and tension than control in terms of physical properties.