• Title/Summary/Keyword: rooting medium

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Seedling Propagation by Stem Cuttings in Yacon

  • Doo, Hong-Soo;Kim, Kyung-Ah;Ryu, Jeom-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.3
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    • pp.184-188
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    • 1998
  • Four experiments were conducted to determine growth regulators, their concentrations and treatment time, rooting medium, and plant parts for optimum seedling propagation by stem cuttings in yacon (Polymnia sonchifolia Poepp. & Endl.). NAA was the most effective on rooting, and followed by IBA 2,4-D, and IAA. Dipping the base of cuttings in 50 or 100 ppm solutions of IBA increased rooting ratios. The 100 ppm solution of IBA was 14% higher than the 50 ppm solution for rooting ratios. Rooting medium with equal volumes of clay loam and sand was the most suitable for rooting of cuttings. Cuttings with both terminal bud and the first node or cuttings with both the first and second nodes were higher in rooting ratios than cuttings with only terminal bud or the first or second nodes. Days to rooting was not affected by plant parts.

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In vitro Root Induction from Shoot Explants of Pear (Pyrus spp.)

  • Jae-Young Song;Jinjoo Bae;Woohyung Lee;Jung-Ro Lee;Mun-Sup Yoon
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.770-777
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    • 2022
  • The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

Micorpropagation of Corylopsis coreana by Thidiazuron Treatment (Thidiazuron 처리에 의한 히어리나무의 기내번식)

  • Kang, Hyo-Jin;Moon, Heung-Kyu;Yi, Jae-Seon
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.263-267
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    • 2003
  • This study was conducted to evaluate the effect of thidazuron(TDZ) on shoot proliferation and growth from axillary buds of 20-years-old Corylopsis coreana. Shoots proliferation was effectively achieved on WPM(Woody Plant Medium) supplemented with 0.03∼0.1mg/L TDZ. The highest shoot number(6.5$\pm$0.7) was obtained on 0.1mg/L TDZ treatment. On the TDZ medium shoots formed as clusters less than 1cm in height and therefore needed to subculture on GA$_{3}$ containing medium to induce elongation. In consecutive cultures, phenolic compounds were excreted at the proximal part of the explants and inhibited growth of the explants. Growth inhibition by the compounds was overcome using liquid and paper bridge culture system. About 60% of the elongated shoots rooted on half- strength MS medium containing IBA. Generally, IBA was mire effective on in vitro rooting than NAA with optimal range of 0.5mg/L to 1.0mg/L. Rooted plantlets were transferred in an artificial soil(vermculite) and acclimatized in high humidity greenhouse condition. Survival rate differed greatly depending on rooting types of the explants. Two types of rooting were observed. The first type was direct rooting from the explants. The second type was callus formation followed by rooting from the callus. The explants showing the 1st type rooting survived can be multiplicated in vitro by TDZ treatment followed by elongation with GA$_{3}$ and rooting with IBA.

Efficient Micropropagation of Pear Germplasm Using Soot Tips and Nodal Explants

  • Yi, JungYoon;Lee, GiAn;Chung, JongWook;Lee, YoungYi;Gwag, JaeGyun;Lee, SeokYoung
    • Korean Journal of Plant Resources
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    • v.28 no.6
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    • pp.690-696
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    • 2015
  • We micropropagated pear (Pyrus species) using shoot tips and nodal explants from three pear genotypes. The ability to establish shoot tip cultures, proliferate shoots, induce rooting, and acclimatize the resulting plantlets are all elements of in vitro micropropagation. Shoots were induced from shoot tips on Murashige and Skoog medium (MS) with five different plant growth regulator combinations. The highest shoot formation rates were achieved for the three genotypes using MS supplemented with 1.0 mg/L N6-benzyladenine (BA) and 0.1 mg/L gibberellic acid (GA3). The maximum shoot number and shoot length for the three cultivars were recorded with 2.0 mg/L BA and 0.2 mg/L indole-3-butyric acid (IBA) in multiplication medium using nodal explants produced from microshoots. Nodal explants with one or two axillary buds cultured for three weeks initiated roots on medium supplemented with various concentrations of 1-naphthaleneacetic acid (NAA) or/and IBA in half-strength MS medium for adventitious rooting. The highest rooting response was with the combination of 0.2 mg/L NAA and 0.2 mg/L IBA. A combination of NAA and IBA resulted in a significant increase in the rooting ratio over NAA or IBA alone. In this medium, the root formation rate according to ranged from 68.9% for the BaeYun No. 3 genotype to 51.8% for the Hwanggeum genotype. We also investigated the influence of the concentration the polyamine phloroglucinol in rooting medium. For all three genotypes, the highest rooting ratio, longest root length, and greatest root number were observed in the treatments with 75-150 mg/L phloroglucinol. Most rooted plants were acclimatized successfully.

Studies on the Value of Briquet Ash as a Component of Rooting Media(Part II) - Influence of Briquet Ash on the Rooting of Stemcuttings in Chrysanthemum morifolium - (삽목용토(揷木用土)로서의 연탄재 이용(利用)에 관(關)한 연구(硏究)(제(第)2보(報)) - Chrysanthemum morifolium의 삽수(揷穗)의 발근(發根)에 미치는 삽목용토(揷木用土) 및 입자(粒子)크기의 영향(影響) -)

  • Suh, Young-Kyo;Ku, Ja-Hyoung
    • Korean Journal of Agricultural Science
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    • v.3 no.1
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    • pp.53-60
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    • 1976
  • The purpose of this study was to evaluate the value of briquet ash as a component of rooting media in Chrysanthemum morifolium. Various rooting media were made in mixture at different ratio and alone using sand, vermiculite and briquet ash as components of soil mixture. In order to investigate the effect of the particle size of briquet ash on rooting of stemouttings, various particle sizes were used as rooting media. Rooting ratio, number of root and root length were examined to evaluate the effect of the various rooting media and the particle sizes of briquet ash. The important conclusions of this study are summerized as follows; 1. Briquet ash was better rooting medium than sand, but it showed more effective result when it was used in mixture with other rooting media. 2. The particle size of briquet ash as a rooting medium to produce a healthy vegetatively propagated plant showed the best result at 2-3mm.

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In vitro Micropropagation and Root Induction of Pear Genetic Resources

  • Jae-young Song;Jinjoo Bae;Woohyung Lee;Jung-ro Lee;Munsup Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.63-63
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    • 2022
  • Pear (Pyrus spp.) is a typical fruit and grown in the temperate climate regions throughout the world. Development of appropriate methods for in vitro propagation and root induction are important to increase the production rate and plant quality rapidly. This study was conducted to find the most appropriate media conditions for in vitro propagation and rooting of three pear cultivars, 'Barttlett', 'BaeYun No.3' and 'Oharabeni'. In vitro propagation was induced on Murashige and Skoog medium (MS) with 2.0 mg/L N6-benzyladenine (BA) and 0.2 mg/L indole-3-butyric acid (IBA) medium. For root induction of these cultivars, the shoot explants of the propagated plants were cultured on two different media containing 1/2 MS medium containing 0.2 mg/L IBA with 15 g/L Sucrose (Rooting Medium 1, RM1) and 1/4 Linsmaier and Skoog medium (LS) medium containing 1 mg/L IBA and 1 mg/L NAA hormone with 7.5 g/L glucose (Rooting Medium, RM2) and after 2 weeks, the plants on the RM2 medium are transferred on RM1 medium (RM2 condition). After nearly seven weeks, percentage of rooting formation were 22.2% in RM1 and 30% in RM2 conditions for Barttlett and 70% in RM1 and 60% in RM2 conditions for Oharabeni cultivars. No differences in these cultivars were observed between RM1 and RM2 conditions. However, BaeYun No.3 cultivar was observed 0% in RM1 and 72.7% in RM2 conditions. This study will help to propagation and root induction of in vitro plants for various pear cultivars.

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Promotion of in vitro growth and rooting of micropropagated shoots in Spathiphyllum floribundum by the addition of liquid medium (액체배지 첨가에 의한 Spathiphyllum floribundum 신초의 기내생육 및 발근 촉진)

  • 한봉희;예봉우;구대회;신지수
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.4
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    • pp.185-188
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    • 2001
  • This experiments were carrid out to examine the effects of liquid medium addition in same vessels on shoot elongation and rooting, and soil survival of plantlets after the shoot cluster sections of Spathiphyllum floribundum 'Cupid' were pre-cultured. The shoot clusters with 3 to 4 small shoots were proliferated on LS medium supplemented with 2.0 mg/L BA for 8 weeks, and then 15 mL of various kinds of liquid medium was added in the same vessels. The addition of 15 mL liquid medium containing l/2 MS macro elements, 50 g/L sucrose and 5.0∼10.0 g/L activated charcoal was significantly stimulated the elongation and rooting of proliferated shoots. The medium addition was resulted in the enhanced soil survival of plantlets.

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Growth Stimulation of In Vitro Shoots by the Post-supplying of Liquid Medium in Anthurium andreanum ′Atlanta′ (액체배지 첨가에 의한 Anthurium andreanum ′Atlanta′의 기내생육 촉진)

  • Han, Bong-Hee;Goo, Dae-Hoe;
    • Journal of Plant Biotechnology
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    • v.30 no.2
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    • pp.185-188
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    • 2003
  • In order to enhance shoot elongation and rooting of Anthurium andreanum 'Atlanta' in vitro, 15mL of liquid media containing various concentrations of activated charcoal, sucrose and MS salts were added in same vessels after small shoots were induced from the calli on mudium supplemented with 10.0mg/L BA and 0.1mg/L 2.4-D. The post-supplying of 15mL liquid medium containing MS macro and micro elements, 30g/L sucrose and 5.0∼10.0g/L activated charcoal was significantly stimulated the shoot elongation and rooting of regenerated shoots from calli. The medium addition was also resulted in the enhanced soil survival, elongation and rooting of plantlets in cultural soil mixed with perlite and vermiculite(1 : 1)

The Promotive Effect of NAA, IBA and Ethychlozate on Rooting Cuttings of Certain Ornamental Plants and Some Physiological Studies. (관상식물 삽목발근에 있어서 NAA, IBA 및 Ethychlozate의 발근촉진효과와 그 생리학적연구)

  • Jeong, Hae-Jun;Gwak, Byeong-Hwa
    • The Journal of Natural Sciences
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    • v.1
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    • pp.115-198
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    • 1987
  • The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

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Effects of Phytohormone and Activated Carbon on the Growth and Rooting of Teratoma Shoot Induced from Crown Gall Callus in Nicotiana tabacum cv. NC 2326 (연초 Crown Gall Callus유래 Teratoma Shoot의 생육과 발근에 미치는 식물호르몬과 활성탄의 영향)

  • Yang, Deok-Chun;Kang, C.K.;Choi, K.T.
    • Journal of the Korean Society of Tobacco Science
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    • v.14 no.1
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    • pp.33-41
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    • 1992
  • These studies were conducted to investigate effects of phytohormone and activated carbon on the growth and rooting of teratoma shoots induced from Nicotiana tabacum cv. NC2326 transformed by Aerobacterium tumefaciens C58. GA was effective for shoot elongation and reduction of multiple shoots from teratoma shoot, however, leaves of teratoma shoot cultured on the medium with GA were pointed. ABA was also effective in promoting shoot elongation, but was not for reduction of multiple shoots. Teratoma shoot cultured on the medium with 1 n activated carbon promoted shoot elongation and inhibited the number of shoots differentiated, but was grown as abnormal shoot. Addition of 1% activated carbon and 0.5mg/l BA to culture media was effective for shoot elongation and reduction of multiple shoot and for formation of round leaves as normal leaves. Though these shoots were inoculated on the rooting medium, they could not from roots but formed multiple shoots. Boric acid, myo-inositol and sucrose were also ineffective on the rooting of teratoma shoots.

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