• Korean journal of applied entomology
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• v.25 no.3 s.68
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• pp.169-173
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• 1986

Ginseng fields abolished by potato rot nematode (Ditylenchus destructor) (heavily infested with the nematode) were treated with selected nematicides after preplanting treatment with fumigants or non fumigant chemicals in the fall of 1982. Suppression of the nematode populations was noted in the fields fumigated by cylon (Cy) and treated with ethoprop (E) or triazophos (T). Also in these treatments, high percentages of shoot sprouting and low nematode damages were observed. In the fields fumigated by Cy, subsequent treatments with nonfumigant nematicides such as E, aldicarb (A), phenamiphos (Ph), oxamyl (O) and carbofuran (C) during cultivation had no effect on the significant decrease of the nematode, compared with Cy alone. No differences in shoot sprouting and symptom appearance were observed; however, survivalities of plants and root weights of 4-year old ginseng were high in A and E or E following preplanting treatment with E. Correlation coefficients of the nematode densities to host responses such as sprouting of shoots, symptom appearance, survivality of plants and weight of 4-year old ginseng roots were significant at and mostly before and after the time periods of survey.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.7
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• pp.791-799
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• 2016

The purpose of this work is to analyse the impact of new accident risks on maritime safety in Korea. The new accident risks have been induced from new/rare or unprecedented events in world maritime transportation, as identified by 46 experts in the previous study. To measure the impact of these new accident risks on maritime safety in Korea, the statistical accident data reported by the Korean Maritime Safety Tribunals (KMST) has been used for calculation, and the concept of Risk Index (RI) = Frequency Index (FI) + Severity Index (SI)established in a Formal Safety Assessment (FSA) by the IMO has also been introduced. After calculating two kinds of weight for FI and SI from the statistical accident data, high ranked scenarios were identified and their relationships between new risks and these scenarios were analysed. The results from this analysis showed, the root cause of the top-ranked scenario to be "developing high technology", which leads to "shorten cargo handling time". These results differed from optimum RCOs such as "business competition" and "crewing problems" which were identified in the previous study.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.367-374
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• 2007

This study was carried out to establish the micropropagation system of Bupleurum latissimum Nakai that is a Korean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callus formation was highest in the floral bud on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D. Especially, callus induced from floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium was more effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%, 13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ BA where percentage of shoot regeneration was 74.1 %, and the number of shoot per explant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg ${\cdot}$ $L^{-1}$ NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plant height, root length and fresh weight were good in the soil for horticulture.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.128-135
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• 2016

This study examined radiation damage and the optimal gamma-ray dose for mutation breeding in oat (Avena sativa L. cv. Samhan). The seed germination rate decreased as the dose increased over 500 Gy. The median lethal dose (LD₅₀) was approximately 392 Gy. The median reduction dose (RD₅₀) for plant height, tiller number, root length, and flash weight was 411, 403, 394, and 411 Gy, respectively. The optimal dose of gamma irradiation for inducing oat mutation appears to be in the range 300-400 Gy. We performed the comet assay to observe nuclear DNA damage induced by gamma-ray irradiation. This assay showed a clear difference with gamma-ray treatments. DNA damage increased temporarily 7 days after treatment depending on the dose, while no significant difference was identified in response to 300 Gy 30 days after the gamma-ray treatments. The growth characteristics of the M₂ generation decreased as the dose increased over 400 Gy.

• Journal of Ginseng Research
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• v.42 no.4
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• pp.447-454
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• 2018

Background: Korean ginseng has been widely evaluated to treat human diseases; however, most studies on Korean ginseng have focused on its root. In this study, polysaccharides [acidic-polysaccharide-linked glycopeptide (APGP) extracted with 90% ethanol and hot water] were prepared from Korean ginseng berries, and their effect on immunosenescence was explored. Methods: The effect of APGP on thymic involution was evaluated by measuring the size of thymi dissected from aged mice. The effect of APGP on populations of immune cells, including natural killer (NK) cells, dendritic cells, age-correlated CD11c-positive B cells, and several subtypes of T cells [CD4-positive, CD8-positive, and regulatory (Treg) T cells] in the thymi and spleens of aged mice was analyzed by fluorescence-activated cell sorting analysis. Serum levels of interleukin (IL)-2 and IL-6 were evaluated by enzyme-linked immunosorbent assay analysis. Profiles of APGP components were evaluated by high-performance liquid chromatography (HPLC) analysis. Results: APGP suppressed thymic involution by increasing the weight and areas of thymi in aged mice. APGP increased the population of NK cells, but showed no effect on the population of dendritic cells in the thymi and spleens of aged mice. APGP decreased the population of age-correlated CD11c-positive B cells in the spleens of aged mice. APGP showed no effect on the populations of CD4- and CD8-positive T cells in the thymi of aged mice, whereas it increased the population of Treg cells in the spleens of aged mice. APGP further decreased the reduced serum levels of IL-2 in aged mice, but serum levels of IL-6 were not statistically changed by APGP in aged mice. Finally, HPLC analysis showed that APGP had one major peak at 15 min (a main type of polysaccharide) and a long tail up to 35 min (a mixture of a variety of types of polysaccharides). Conclusion: These results suggested that APGP exerted an anti-immunosenescent effect by suppressing thymic involution and modulating several types of immune cells.

• Journal of Haehwa Medicine
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• v.13 no.2
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• pp.205-212
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• 2004

Now many sustitution and false articles is used in korea instead of heshouwu. To use heshouwu correctly, we will make a quilitative certificational plan of heshouwu to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1) Source of plant Heshouwu is a root tuber of a perennial herb Polygonum multiflorum Thunberg(Family : Polygonaceae). 2) Harvest After planting 3-5 yaers, harvesting in an autumn, washin clean the mud, a big heshouwu cut off a half or section, dry in sunny place or at a little fire. When harvesting, we harvest only a big thing, a small thing transfer a field, after culturing of 1-2 years, harvest at big roots. Harvesting is done usually in an autumn after 3 years. When collecting a seed, we must harvest a heshouwu the next year. 3) Process We must process heshouwu at the decoction of black beans, heshouwu suck in the decoction of black beans, heat with steam in an iron pot. Black beans is used every 100 kg of heshouwu. 4) Quility (1) Funstional standards It is good that weight is heavy and outer skin is yellow-brown, section surface is light red color, powdery and has a figure such as clouds in section. (2) Physicochemical standards Heshouwu expesses a various chang of components in process of working. We think that it need to add a standard of detection about 2,3,5,4'-tetrahrdroxystilbene-2-O-${\beta}$ -D-glucoside in a current authentic document which is a water-soluble component of heshouwu. It must that Dry on loss is less than 14.0%, content of ash is less than 5.0%, Content of acid-nonsoluble ash is less than 1.5%, Content of extract is more than 17.0%. A fixed quantity of 2,3,5,4'-tetrahrdroxystilbene-2-O-${\beta}$ -D-glucoside is more than 1.0%. Contens of heavy metal has to detect less than 30 ppm and there is no reminding agriculural medince.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.25-31
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• 2017

Objectives : Mori Radicis Cortex (MRC), the root epidermis of Morus alba L., has been traditionally used to treat lung-related diseases in Korean Medicine. The common of MRC is Mulberry bark Morus bark, and it's pharmaceutical properties and taste are known as sweet and cold, and it promotes urination and reduce edema by reducing heat from the lungs and soothe asthma. In the present study, anti-apoptotic mechanism of MRC in middle cerebral artery occlusion (MCAO) model in mice. Methods : Two-hundred grams of MRC was extracted with methanol at room temperature for 5 days, and this was repeated one time. After filtration, the methanol was removed using vacuum evaporator, then stored at $-20^{\circ}C$ until use. C57BL/6 male mice were housed in an environment with controlled humidity, temperature, and light cycle. In order to determine beneficial effects of MRC on ischemia induced brain damage, infarct volume, neurological deficit scores, activities of several apoptosis-related proteins such as caspase-8, -9, Bcl-xL in MCAO-induced brains of mice were analyzed. Mice in MRC-treated groups were orally administered 30, 100, or 300 mg/kg of body weight for three consecutive days before commencing the MCAO procedure. Results : Pre-treatment of MRC significantly reduced infarct volume in MCAO subjected mice applied with 300 mg/kg of MRC methanol extract, and MRC effectively inhibited Bcl-xL reduction and caspase-9 activation caused by MCAO-induced brain damage. Conclusions : MRC showed neuro-protective effects by regulating apoptosis-related protein signals, and it can be a potential candidate for the therapy of ischemia-induced brain damage.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.601-610
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• 1998

This study was carried out to investigate the possibility of supplemental UV-B application to the hardening phase of container-grown Betula platyphylla seedlings. The containerized seedlings were grown in a growth chamber for four months and then treated with UV-B(UV-$B_{BE}$ $3.2KJ\;m^{-2}\;day^{-1}$ and $5.2KJ\;m^{-2}\;day^{-1}$) radiation and water stress regime(irrigation in one week interval) for four weeks. The differences in growth and physiological responses of the seedlings before and after the treatments were analyzed. UV-B radiation and water stress reduced height growth and leaf dry mass accumulation of the seedlings. The root collar diameter growth was reduced by UV-B radiation but increased by water stress. The reduction in leaf dry weight by UV-B radiation and water stress reduced T/R ratio of the seedling. The reduction in T/R ratio was the most apparent by water stress. Chlorophyll index observed by a chlorophyll meter was the lowest in the $5.2KJ\;m^{-2}\;day^{-1}$ of UV-B radiation, and those in the $3.2KJ\;m^{-2}\;day^{-1}$ and water stress were similar. UV-B radiation and water stress reduced both water content in the seedlings and leaf water potential, and increased leaf osmatic pressure. The water content of leaves and shoots was reduced more rapidly by UV-B radiation than by water stress treatment. In conclusion, growth responses and physiological changes in water relation by supplemental UV-B radiation which was applied to the hardening phase of container-grown Betula platyphylla seedlings were similar results to the water stress treatment.

• Journal of Korean Society of Forest Science
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• v.104 no.1
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• pp.43-49
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• 2015

The production of high quality seedlings is a very important phase in silvicultural systems for successful reforestation or restoration. The purpose of this study was to quantitatively measure both growth performances and nutrient responses of Fraxinus rhynchophylla, Pinus densiflora, and Pinus koraiensis seedlings, which are commercially planted in Korea, according to the different types of soil improvement treatments. We applied soil brought (hereafter 'brought'), subsoil inversion (hereafter 'subsoil'), and mixture of brought soil with soil on nursery bed (hereafter 'mixing') in a permanent national nursery. Silt and clay contents were the highest at the subsoil treatment and organic material, soil nitrogen and phosphorus concentrations were the lowest at the brought treatment. The growth of F. rhynchophylla was the lowest at the subsoil treatment, but there were no significant differences among treatments. There were significant differences in only root nutrient concentrations of F. rhynchophylla among treatments: nitrogen, phosphorus, and potassium concentrations were the lowest at the subsoil or brought treatment. Mixing treatment increased N contents with deduction of N concentrations ('dilution') because of more dry weight increase compared with the amount of N uptake. This study suggested mix of brought soil with soil on a nursery bed in a permanently used nursery can economically be an effective technique to improve soil quality.

• Korean journal of applied entomology
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• v.27 no.3
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• pp.149-158
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• 1988

Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.