• Proceedings of the KACD Conference
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• 2002.11a
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• pp.720-720
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• 2002

A number of investigations have shown that the presence of bacteria is prerequisite for developing pulpal and/or periradicular pathosis. Depending on the stage of pulpal pathosis, various species of bacteria can be cultured from infected root canals. Kakehashi et al. showed that exposure of pulpal tissue in germ-free rats was characterized by minimal inflammation and dentinal bridging while exposure of pulpal tissue in conventional rats with normal oral flora was characterized by pulpal necrosis, chronic inflammation, and periapical lesions. Currently used methods of cleaning and shaping, especially rotary instrumentation techniques, produce a smear layer that covers root canal walls and the openings to the dentinal tubules. The smear layer contains inorganic and organic substances that include fragments of odontoblastic processes, microorganisms, their by products and necrotic materials. Because of its potential contamination and adverse effect on the outcome of root canal therapy, it seems reasonable to suggest removal of the smear layer for disinfection of the entire root canal system. Presence of this smear layer prevents penetration of intracanal medications into the irregularities of the root canal system and the dentinal tubules and also prevents complete adaptation of obturation materials to the prepared root canal surfaces. Removal of the smear layer by an intracanal irrigant and placement of an antibacterial agent in direct contact with the content of dentinal tubules should allow disinfection of this complex system and better outcome for the root canal therapy. A new solution, which was a mixture of a tetracycline, an acid, and a detergent(MTAD), was developed in the Department of Endodontics, Dental School. Lorna Linda University, USA. It has been demonstrated that MTAD was an effective solution for the removal of the smear layer and does not significantly change the structure of the dentinal tubules when used as a final irrigant in conjunction with 1 % NaOCl as a root canal irrigant. Studies are in progress to compare the anti- microbial properties of this newly developed solution with those of sodium hypochlorite and EDTA that are currently used to irrigate the root canals and remove the smear layer from the surfaces of instrumented root canals.canals.

• Geotechnical Engineering
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• v.7 no.2
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• pp.51-66
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• 1991

In the stability analysis of hillside slopes, the roots of vegetation have been considered to act as a soil reinforcement. In order to predict the amount of increase in soil shear resistance, produced by tensile strength of roots that intersect a potential slip surface in hillside slopes, new soil -root interaction models are proposed in this paper. For this purpose, firstly, laboratary teats and in-situ tests wert performed on soil-root systems, and experimental results were compared with a couple of soil-root interaction models which had been proposed by Gray, Waldron, and Wu etc. Based on this comparison, a new soil-root interaction model is proposed. Secondly, a probabilistic soil-root model is proposed based on statistical analysis considering random nature of root distribution, root characteristics, and soil-root interactions. Finally, to examine the effect of this root reinforcement system on stability of hillside slopes, a simple three-dimensional stability analysis was performed, and it was shown that root reinforcement had a significant stabilizing influence on shallow slips rather than deep slips in hillside slopes.

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.10a
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• pp.175-176
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• 2003

• YAKHAK HOEJI
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• v.56 no.2
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• pp.108-115
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• 2012

The hyperpolarization-activated current ($I_h$) is an inward cation current activated by hyperpolarization of the membrane potential and plays a role as an important modulator of action potential firing frequency in many excitable cells. In the present study we investigated the effects of extracellular divalent cations on $I_h$ in dorsal root ganglion (DRG) neurons using whole-cell voltage clamp technique. $I_h$ was slightly increased in $Ca^{2+}$-free bath solution. BAPTA-AM did not change the amplitudes of $I_h$. Amplitudes of $I_h$ were decreased by $Ca^{2+}$, $Mg^{2+}$ and $Ba^{2+}$ dose-dependently and voltage-independently. Inhibition magnitudes of $I_h$ by external divalent cations were partly reversed by the concomitant increase of extracellular $K^+$ concentration. Reversal potential of $I_h$ was significantly shifted by $Ba^{2+}$ and $V_{1/2}$ was significantly affected by the changes of extracellular $Ca^{2+}$ concentrations. These results suggest that $I_h$ is inhibited by extracellular divalent cations ($Ca^{2+}$, $Mg^{2+}$ and $Ba^{2+}$) by interfering ion influxes in cultured rat DRG neurons.

• Journal of Korean Dental Science
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• v.7 no.1
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• pp.1-5
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• 2014

Purpose: Capseal I and Capseal II are calcium silicate and calcium phosphate based experimental root canal sealers. This study sought to evaluate the biocompatibility and mineralization potential of Capseal I and Capseal II. Materials and Methods: The biocompatibility and mineralization related gene expression (alkaline phosphatase [ALP], bone sialoprotein [BSP], and osteocalcin) of Capseal I and Capseal II were compared using methylthiazol tetrazolium assay and reverse transcription-polymerization chain reaction analysis, respectively. The results were analyzed by Kruskal-Wallis test. A P-value of <0.05 was considered significant. Result: Both Capseal I and Capseal II were favorable in terms of biocompatibility, influencing the messenger RNA expression of ALP and BSP. Conclusion: Within the limitation of this study, Capseal is biocompatible, with mineralization promoting potential; thus, it could be a promising root canal sealer.

• Journal of Ecology and Environment
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• v.35 no.2
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• pp.73-78
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• 2012

$Calotropis$ $procera$ L. is a perennial shrub distributed in saline areas of deserts of South Asia. Salt stress is a very challenging subject in arid and semi-arid areas. Germination stage is very sensitive and many plants do not germinate in saline soil. The objective of this study was identifying the salinity effect on seed germination of $Calotropis$ $procera$ L. The experimental design was a complete randomized block design with NaCl and $CaCl_2$ at five levels of isobar concentrations: 0.0, -0.01, -0.05, -0.1, and -0.15 MPa. Osmotic potential had significant effects ($P$ < 0.01) on germination percentage, germination rate, shoot length, root length, and seedling dry weight. All seedling characteristics decreased with decrease in osmotic potential. Shoot length and root length decreased more than the seedling characteristics. Germination was completely inhibited in -0.1 Mpa. Priming with NaCl and $CaCl_2$ (-0.1 MPa) for four days had significant effects ($P$ < 0.01) on the germination percentages. Priming improved the seedling characteristics in all samples, especially in -0.05 Mpa, but a decrease with decrease in osmotic potential.

• Journal of Ginseng Research
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• v.4 no.2
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• pp.197-221
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• 1980

Habitat observation, cultural experience of old and present plantation, weather factors in relation to crop stand and water physiology of root and leaf were reviewed. According to habitat observation ginseng plants love water but plate wit talus well grow at drained place with high moisture content in air and soil while ginseng plants were not found in dry or wet place. According to cultivation experience ginseng plants require abundant water in nursery and main field but most old planters believe that ginseng plaints are draught-loving thus require little water. The experience that rain especially in summer i.e unfavorable might be due to mechanical damage of leaves arid leaf disease infection, or severe leaf fall which is caused by high air temperature and coinsided with rain. According to crop stand observation in relation to weather factors abunsant water increased each root weight but decreased total yield indicating tile increase of missing root rate. Rain in summer was unfavorable too. Though rain in June was favorable for high yield general experience that cloudy day and rain were unfavorable might be due to low light intensity under shade. Present leading planters also do loot consider the importance of water in main field. Water content is higher in top than in root and highest in central portion of root and in stem of top. For seedling the heavier the weight of root is tile higher the water content while it reveries from two years old. Water potential of intact root appeared to be -2.89 bar suggesting high sensitivity to water environment. Under water stress water content severly decreased only in leaf. Water content of leaf appeared to be 78% for optimum, below 72% for functional damage and 68% for perm anent wilting. Transpiration or curs Principally through stomata in lower side of leaf thus contribution of upper side transpiration decreased with the increase of intensity. Transpiration is greater in the leaves grown under high light intensity. Thus water content is lower with high light inte nsity under field condition indicating that light is probable cause of water stress in field. Transpiration reached maximum at 10K1ut The decrease of transpiration at higher temperature seems to be due to the decrease of stomata aperture caused by water stress. Severe decrease of photosynthesis under water stress seems to be principally due to functional damage which is not caused by high temperature and Partly due to poor CO2 supply. Water potential of leaf appeared to be -16.8 bar suggesting weakness in draught tolerance. Ginseng leaves absorb water under high humidity. Water free space of leaf disc is %mailer than that of soybean leaf and water uptake appears to be more than two steps.

• Journal of Microbiology and Biotechnology
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• v.28 no.4
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• pp.551-560
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• 2018

Bellflower root (Platycodon grandiflorum), which belongs to the Campanulaceae family, is a perennial grass that grows naturally in Korea, northeastern China, and Japan. Bellflower is widely consumed as both food and medicine owing to its high nutritional value and potential therapeutic effects. Since foodborne disease outbreaks often come from vegetables, understanding the public health risk of microorganisms on fresh vegetables is pivotal to predict and prevent foodborne disease outbreaks. We investigated the microbial communities on the bellflower root (n = 10). 16S rRNA gene amplicon sequencing targeting the V6-V9 regions of 16S rRNA genes was conducted via the 454-Titanium platform. The sequence quality was checked and phylogenetic assessments were performed using the RDP classifier implemented in QIIME with a bootstrap cutoff of 80%. Principal coordinate analysis was performed using the weighted Fast UniFrac distance. The average number of sequence reads generated per sample was 67,192 sequences. At the phylum level, bacterial communities from the bellflower root were composed primarily of Proteobacteria, Firmicutes, and Actinobacteria in March and September samples. Genera Serratia, Pseudomonas, and Pantoea comprised more than 54% of the total bellflower root bacteria. Principal coordinate analysis plots demonstrated that the microbial community of bellflower root in March samples was different from those in September samples. Potential pathogenic genera, such as Pantoea, were detected in bellflower root samples. Even though further studies will be required to determine if these species are associated with foodborne illness, our results indicate that the 16S rRNA gene-based sequencing approach can be used to detect pathogenic bacteria on fresh vegetables.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.234-242
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• 2011

BACKGROUND: Cultivation of winter cover crops is strongly recommended to increase land utilization efficiency, animal feeding material self-production, and to improve soil and environmental quality. METHODS AND RESULTS: Four major winter crops (barley, Chinese milk vetch, hairy vetch, and rye) having different C/N ratio were seeded in silt loam paddy soil in the November 2007 and the aboveground biomass was harvested on the late May 2008 to evaluate its effectiveness as green manure, and root biomass distribution was characterized at the different depth (0-60 cm) to study its effect on physical properties and carbon sequestration in soil. During this experiment, the naturally growing weed in the rice paddy soil in Korea, short awn foxtail (Alopecurus aequalis Sobol), was considered as control treatment. Above-ground biomass of all cover crops selected was significantly higher than that of the control treatment (2.8 Mg/ha). Comparatively higher above-ground biomass productivity of rye and barley (15.8 and 13.5 Mg/ha, respectively) suggested that these cover crops possibly had the highest potential as a green manure and animal feeding material. Root biomass production of different cover crops followed the same trend as that for their above ground biomass. Rye (Secale cereal) might have the highest potential for soil C accumulation (7893 C kg/ha) by root biomass development, and then followed by barley (6985 C kg/ha), hairy vetch (6467 C kg/ha), Chinese milk vetch (6671 C kg/ha), and control (5791 C kg/ha). CONCLUSION(s): Cover crops like rye and barley having high biomass productivity might be the most effective winter cover crops to increase organic carbon distribution in different soil aggregates which might be beneficial to improve soil structure, aeration etc. and C sequestration.

• Restorative Dentistry and Endodontics
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• v.48 no.1
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• pp.8.1-8.8
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• 2023

Objectives: This study was designed to evaluate the parameters of bonding performance to root dentin, including push-out bond strength and dentinal tubular biomineralization, of a hydraulic bioceramic root-end filling material premixed with dimethyl sulfoxide (Endocem MTA Premixed) in comparison to a conventional powder-liquid-type cement (ProRoot MTA). Materials and Methods: The root canal of a single-rooted premolar was filled with either ProRoot MTA or Endocem MTA Premixed (n = 15). A slice of dentin was obtained from each root. Using the sliced specimen, the push-out bond strength was measured, and the failure pattern was observed under a stereomicroscope. The apical segment was divided into halves; the split surface was observed under a scanning electron microscope, and intratubular biomineralization was examined by observing the precipitates formed in the dentinal tubule. Then, the chemical characteristics of the precipitates were evaluated with energy-dispersive X-ray spectroscopic (EDS) analysis. The data were analyzed using the Student's t-test followed by the Mann-Whitney U test (p < 0.05). Results: No significant difference was found between the 2 tested groups in push-out bond strength, and cohesive failure was the predominant failure type. In both groups, flake-shaped precipitates were observed along dentinal tubules. The EDS analysis indicated that the mass percentage of calcium and phosphorus in the precipitate was similar to that found in hydroxyapatite. Conclusions: Regarding bonding to root dentin, Endocem MTA Premixed may have potential for use as an acceptable root-end filling material.