• Journal of Crop Science and Biotechnology
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• 제11권2호
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• pp.91-100
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• 2008

Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism(SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer(FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci(QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 $F_2$ plants from the cross of PI 96354$\times$Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot(FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 hours without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean.

• The Plant Pathology Journal
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• 제38권6호
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• pp.583-592
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• 2022

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

• 식물병연구
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• 제19권1호
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• pp.25-30
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• 2013

국내에서 시판되고 있는 토마토 36품종을 이용하여 국내 시설재배지에서 가장 많이 분포하고 있는 두 종의 뿌리혹선충, Meloidogyne arenaria와 M. incognita에 대한 저항성을 검정하였다. 방울토마토 중에서는 '텐텐', '캐딜락', '큐티', '스위트', '뽀또', '리코핀-9' 등 6품종, 완숙토마토 중에서 '러브리240', '도테랑다이아', '큐피랑', '도테랑마스터', '슈퍼도태랑', '도태랑시즌', '마이로꾸', '호용' 등 8품종, 대목은 '스페셜', '파이팅', '마그네트' 등 3품종이 두 종류의 뿌리혹선충에 모두 강한 저항성이었다.

• The Plant Pathology Journal
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• 제37권6호
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• pp.596-606
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• 2021

Soil-borne diseases are the major problems in mono cropping. A mixture (designated LTM-m) composed of agricultural wastes and a beneficial microorganism Streptomyces saraceticus SS31 was used as soil amendments to evaluate its efficacy for managing Rhizoctonia solani and root knot nematode (Meloidogyne incognita). In vitro antagonistic assays revealed that SS31 spore suspensions and culture broths effectively suppressed the growth of R. solani, reduced nematode egg hatching, and increased juvenile mortality. Assays using two Petri dishes revealed that LTM-m produced volatile compounds to inhibit the growth of R. solani and cause mortality to the root knot nematode eggs and juveniles. Pot and greenhouse tests showed that application of 0.08% LTM-m could achieve a great reduction of both diseases and significantly increase plant fresh weight. Greenhouse trials revealed that application of LTM-m could change soil properties, including soil pH value, electric conductivity, and soil organic matter. Our results indicate that application of LTM-m bio-organic amendments could effectively manage soil-borne pathogens.

• 한국응용곤충학회지
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• 제43권1호
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• pp.27-34
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• 2004

더덕(Codonopsis lanceolata) 재배지에서 당근뿌리혹선충의 환경친화적 방제법을 구명코자 방제실험을 수행한 결과 당근뿌리혹선충 방제 실험에 사용된 방제제들(Bacillus thuringiensis, Paecilomyces lilacinus, fosthiazate, 한약제 추출물) 중 더덕의 발아를 향상시키는 것을 Bt와 한약제 추출물이었고, 억제시키는 것은 fosthiazated였다. Pot에서 미생물제에 의한 당근뿌리혹선충 밀도억제 효과는 Bt와 Paecilomyces lilacinus 모두 우수하였으나 fosthiazate 보다는 떨어지는 경향이었다. 또한 한약제 추출물도 방제효과가 우수하였다. 포장실험에서도 Paecilomyces lilacinus와 fosthiazate 처리 시 수확량이 가장 많았으며 수확된 더덕의 품질도 가장 우수하였다. 한약제추출물도 무처리에 비하여 더덕의 수량 증대에 효과가 있었고, 뿌리혹선충 감염지에서 키운 더덕을 이식한 것이 비감염지에서 키워 이식한 더덕에 비하여 수량 증가율이 저조하였다.

• 식물병연구
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• 제7권1호
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• pp.49-55
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• 2001

남부 시설재배지에서 서식하는 뿌리혹선충의 종류와 race를 조사하고 그들에 대한 간이동정법을 제시하였다. 남부 시설재배지에는 Meloidogyne arenaria race 2(59%), M. incognita race 1(23%), M. incognita 미동정 race(18%)가 분포하고 있었음, 우점종은 땅콩뿌리혹선충(M. arenaria) race 2 였다. 유충의 길이는 M. arenaria가 411$\mu\textrm{m}$(306-503$\mu\textrm{m}$), M. incognitark 384$\mu\textrm{m}$(312-488$\mu\textrm{m}$)로 두 종의 범위가 겹치고 있어 종 동정에 참고하기 어려웠으며, M. arenaria와 M. incognita의 종 동정에 가장 쉽게 이용할수 있는 특징은 암컷의 머리에 있는 Excretory pore 위치였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
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• pp.18.2-18
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• 1999

• The Plant Pathology Journal
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• 제36권4호
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• pp.384-384
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• 2020

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1510-1515
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• 2014

Lysobacter capsici YS1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. In this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of YS1215, and its ovicidal activity was investigated. Purification and identification of lactic acid were performed by a series of column chromatographies and identified by $^1H$ and $^{13}C$ NMR spectra and GC-MS analysis. Our results showed that bacterial culture filtrate containing lactic acid significantly inhibited egg hatching. The lowest egg hatch rate (5.9%) was found at a high concentration ($25 {\mu}l/ml$) of lactic acid at 5 days after incubation, followed by 20 (15.2%), 15 (23.7%), 10 (29.8%), and $5(36.4%){\mu}l/ml$, while egg hatching in the control (sterile distilled water) was 44.5%. This is the first report of lactic acid as an ovicidal compound, and it may be considered as an alternative of chemical pesticide against root-knot nematodes.

• 한국토양동물학회지
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• 제6권1_2호
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• pp.33-36
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• 2001

작약계통에 대한 당근뿌리혹선충의 저항성검정에서, 의성작약, 태백작약 도서종, EP368, EP512는 효충의 밀도가 토양 300m1당 121-173마리로 높았으며 사벌종, 웅수종, 김천종, Pl23에서는 21-60마리로 타계통 보다 기생밀도가 낮았다. 공신된 계통별 작약 뿌리의 부패정도는 용수종, 김천종에서 경징하였으며, 선충의 기생이 많았던 의성작약, 태백작약 Ep368, Ep512는 선충의 기생밀도도 높았으며 뿌리 부패율이 아주 높았다. 작약계통별 당근뿌리혹선충에 대한 저항성정도를 보면 의성작약, 태백작약 용수종, 도서종, EP368, EP512는 난낭의 형성이 많아 당근뿌리혹선충에 감수성이였고, 사벌종과 김천종, Pl23, EP337, EP425는 난낭형성이 매우 낮아 저항성을 나타내었다.