• Horticultural Science & Technology
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• v.34 no.2
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• pp.282-293
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• 2016

Root-knot nematodes (Meloidogyne spp.) are major plant pathogens that cause reductions in yield and quality of several solanaceous crops, including pepper (Capsicum spp.). These losses can be averted through planting of resistant cultivars. Plants are defined as resistant when they suppress nematode reproduction. In this study, the resistance degrees of 102 commercial cultivars of chili pepper (Capsicum annuum) to a root-knot nematode, Meloidogyne incognita, were evaluated by comparing the number of egg masses on their roots to those of 'PR huimangchan', a highly susceptible cultivar that exhibited the most egg masses of the chili pepper cultivars evaluated. Among these cultivars, forty-four (43.1%) showed resistance to M. incognita and eighteen (17.6%) were moderately resistant. The other cultivars (39.3%) were determined to be susceptible. For further study, six chili pepper cultivars (i.e., Gangryeokjosenggeon, Shinsegae, Muhanjilju, PR Bulrocho, PR Huimangchan, and Jjang) with different levels of resistance to the nematode were selected. Changes in resistance of the six cultivars under several conditions, such as inoculum concentration, plant growth stage, and cultivation period after transplanting were investigated. We found that an efficient screening method for resistance of chili pepper to M. incognita is to transplant the chili pepper seedlings 7 days before inoculation, to inoculate 28-day-old plants with M. incognita by loading 5,000 eggs per plant into the pot of soil, to cultivate the plants in a greenhouse ($25{\pm}5^{\circ}C$) for 45-60 days, to measure the number of egg masses on roots of the seedlings, and then to determine the resistance response of the plants by comparing the number of egg masses on the roots with a reference-susceptible cultivar 'PR huimangchan'.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.261-265
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• 1987

Three diffeerent types of symptoms were observed according to the pathogen associated with the rhizomes. The maximum rotting was observed in case when Pythium aphanidermatum was inoculated first followed by Fusarium solani. There was no interaction in case of root knot nematode Meloidogyne incognita and Pythium aphanidermatum. Average per cent germination of the rhizomes were increased significantly in each treatment and maximum in case of Alliette(.25%). The per cent pre & post drenching rotting was minimum in case of Alliette, Burgandy mixture, Dithane-M 45 and Difolatan. These fungicides also increase the yield of rhizome significantly.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.968-975
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• 2018

In the course of screening for microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against the root-knot nematode Meloidogyne incognita, along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% while also increasing root and shoot weights by 251% and 160%, respectively, compared with an untreated control. The cell-free culture filtrate of the HK169 culture killed all juveniles of M. incognita within 48 h. In addition, the nematicidal activity of the culture filtrate was dramatically reduced by a protease inhibitor, suggesting that proteolytic enzymes contribute to the nematicidal activity of HK169. In order to obtain genomic information about the HK169 isolate related to its nematicidal and plant growth-promoting activities, we sequenced and analyzed the whole genome of the HK169 isolate, and the resulting information provided evidence that the HK169 isolate has nematicidal and plant growth-promoting activities. Taken together, these observations enable the future application of E. asburiae HK169 as a biocontrol agent for nematode control and promote our understanding of the beneficial interactions between E. asburiae HK169 and plants.

• Journal of Life Science
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• v.17 no.12
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• pp.1605-1609
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• 2007

The created microbial pesticide was used to examine its effects using the inside-pot test method. The selected microbial pesticide KBC3017 particle pesticide manufactured by using Monacrosporium thaumasium was used in the farm-house outdoor test to find the optimum consistency and its effects. The more amount used, the better effect it showed. However, the optimum consistency was 2% and the KBC3017 particle pesticide for which the diatomite and raw jade powder were used as an increaser, when used 2% level of the total amount of soil, showed 71% effect on nematode prevention. The root and the stem of crops were better compared to those without any pesticide used.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.162-168
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• 2012

An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Bacillus subtilis C-9, which degrade the collagen and gelatin, were isolated from horticulture plantation soil in Kyungpook Sungju-gun Seonnam-myun and Chungnam Gongju-gun Woosung-myun to develop biological nematode pesticide. When $5,000mg\;kg^{-1}$ of A. thaumasia Nema-1 nematicide powder ($7.0{\times}10^3cfu\;g^{-1}$) was treated to pot including Meloidogyne incognita, the number of nematode's egg mass, which is a index of nematicidal activity, decreased to 35% compared to control. While the number of nematode's egg mass decreased to 67% by treating the nematicide powder mixture of $5,000mg\;kg^{-1}$ Nema-1 and B. subtilis C-9 ($8.5{\times}10^5cfu\;g^{-1}$). Furthermore the number of nematode's egg mass of the mixture containing cinnamon extract $10mg\;kg^{-1}$, each $5,000mg\;kg^{-1}$ of Nema-1 and C-9 nematicide powder was decreased to 84%, comparing to the result showed the number of nematode's egg mass decreased to 24%, by the treatment of chemical nemato pesticide Fosthiazate $24mg\;kg^{-1}$. These results suggested the mixture of microorganisms and plant extract was more effective biological nematicide than the case of only microorganism or plant extract for nematode control.

• Research in Plant Disease
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• v.7 no.1
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• pp.42-48
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• 2001

The effect of cultural, physical and chemical control methods on the population density of Meloidogyne arenaria second-stage juveniles (J2) and on fruit yields of oriental melon was investigated at Seongju Fruit Vegetable Experiments Station, Kyungpook province, Korea, for two years from 1999 to 2000. Crops used in a rotation prior to Oriental melon were rice, corn, sesame, and green onion. The physical methods used were either solarization, soil addition or soil drying, and a nematicide, fosthiazate of granular formula, was used as the chemical method, applying at a rate of 300g a. i./10 a. Growing rice in the rotation, solarization, and soil addition controlled the nematode disease most effectively, reducing the number of J2 by 90% and increasing fruit yields two times. However, the effects of these control methods on the J2 population were limited to the early growing season; the J2 population increased later, suggesting that additional control practices may be needed in the following season. The next effective control methods were use of corn in the rotation, the nematicide application, and soil drying. The nematicide application was effective only for the early fruit yield, but neither for the late nor for the total yields. Use of sesame or green onion in the rotation was not effective in controlling the nematode.

• Korean journal of applied entomology
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• v.40 no.1
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• pp.89-95
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• 2001

Effects of application method of eight nematicides on reproduction of iweloidogyne arenaria were tested in pot. In pre-treatment, cadusafos , dazomet, fosthiazate reduced over 90% in number of egg masses of M. arenaria at recommended rate . Carbofuran showed similar reduction only at three times of recommended rate. Benfuracarb was the least effective. Post-treatments, in general, were less effective than pre-treatment except fostiazate which reduced root egg mass of iW. arenaria as much as pre-treatment at recommended rate . Systematic nematicide, fosthiazate has potential as post-treatment nematicide.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.93-106
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• 2003

Soilborne diseases pose a serious problem for mulberry cultivation during nursery plantation and established gardens, which cause severe loss in revenue generation of mulberry growers as compared to foliar diseases. Various soilborne diseases affect mulberry. Among them, root knot and root rot affect the established plantation resulting in severe loss in leaf yield apart from deterioration in leaf quality, which is a pre-requisite in successful sericulture to get the good quality of cocoons. Besides, stem-canker, cutting rot, collar rot and die-back, affect the initial establishment and survivability of mulberry plantation in nursery. The problem is difficult to handle, due to the complex nature of the diseases and also involvement of various biotic and abiotic factors. This is compounded by the occurrence of disease complex (especially nematode ＋ soilborne pathogenic microbes) in established mulberry gardens, which facilitates quick spread of the disease and enhance the plant mortality, resulting substantial loss in leaf yield. Therefore, prevention and timely control measures need to be taken up to protect the mulberry plants from different soilborne plant pathogens. In this review article, symptomatology, epidemiology, disease cycle and control measures of soilborne diseases of mulberry are discussed.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.195-201
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• 1987

Insects and nematodes associated with crops growing in plastic film houses were surveyed throughout the southern part of Korea at Jinju, Jiphyeon, Geumsan, Hapcheon, Changyeong, Namji, Milyang, Kimhae, Busan, Sooncheon and Gwangyang from December of 1984 to December of 1985. The phytonematodes representing six families, nine genera and nine species, and the insects representing four orders, seventeen families, twenty-three genera and twenty-four species were identified. The mite also caused problem on the leaves of strawberry and watermelon. Of these Meloidogyne incognita and Aphis gossyphii were most important ones. Aphelenchoides fragariae and M. hapla, however dominant nematodes on strawberry. M. incognita was always detected from pepper plants which were heavily infected with Phytophthora capsici. Tomato roots were readily infected with root-knot nematodes in non-sterilized upland surface soil. However, tomato were growing-vigorouly free from nematode damage in the upland surface soil treated by nematicide or in the paddy soil. A few galls were developed even in the upland subsoil at 60cm below surface. Soil salinity affected profoundly the host-root-knot nematode interaction: the numerous galls were developed on the tomato roots at EC $1mS/cm^2$ followed by 4mS and 2mS, but few galls were at 6mS.

• Research in Plant Disease
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• v.20 no.4
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• pp.264-269
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• 2014

The effect of biological control of the root-knot nematode, Meloidogyne incognita, on cucumber and lettuce was evaluated with green manure crop species in greenhouse. Nematicidal effect of sudan grass cultivation in cucumber greenhouse was comparable to that of chemical treatment with fosthiazate GR, showing the high activity of 88.6%. Sudan grass cultivation in lettuce greenhouse significantly reduced the number of M. incognita in soil, showing 93.5% of nematiidal activity. In addition, since growth of sudan grass was superior to other green manure crop species, it is considered that cultivation of sudan grass is proper to control M. incognita in greenhouse.