• Title/Summary/Keyword: root knot nematode

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Biological control of the Nothern Root-knot Nematode, Meloidpgyne hapla with Plant Extract (식물의 추출물을 이용한 당근뿌리혹선충의 생물적방제)

  • 김형환;추호렬;박정규;이상명;김준범
    • Korean journal of applied entomology
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    • v.37 no.2
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    • pp.119-206
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    • 1998
  • Nematicidal potential of some plant extracts were evaluated for the control efficacy of the northern root-knot nematode, Meloidogyne hapla on tomato (Lycopersicon esculentum) in pot. Tagetes patula, Zoysia japonica, Rhus sylvestris, R. chinensis, and Allium cepu were used. Leaf or root extracts were prepared at the rate of undiluted and diluted to 2, 4, 8, or 16 times with distilled water and treated simultaneously, ahead or later tomato planting. Pre-treatments of extracts were more effective than simultaneous or post-treatments, and the number of egg masses was different according to concentrations. M. hapla was less infected when the T. patula was planted at 15 days ahead tomato planting. Leaf or root extracts of T. patulu reduced damages of M. hapla significantly in all treatments. Leaf extract was more effective than root extract; the number of egg masses was 2.2 and 5.5 in 5-days pre-treatment of leaf or root extracts while 129.4 in control. In Z. japonica treatment, the number of egg masses was 87.2 in control, 21 in undiluted concentration of leaf extract and 28.4 in diluted concentration as 2 times. Leaf extracts of R. sylvestris, R. chinensis and A. cepa were also very effective against M. Izapla. The number of egg masses of M. hapla was 1.6, 1.6, and 6.2 in 5-days pre-treatments of R. sylvestris, R. chinensis and A. cepa, respectively while it was 193.6 in control. Less egg masses of M. hapla were detected in higher concentrations than in lower concentrations of all the plant extracts.

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Plant-parasitic Nematodes Associated with Commercial Orchards of Passion Fruit and Adjacent Cerrado Vegetation in the Brazilian Federal District

  • Castro, Ana Paula Gomes De;Goulart, Alexandre Moura Cintra;Andrade, Ednalva Patricia De;Cares, Juvenil Enrique;Carvalho, Daniel Diego Costa
    • The Plant Pathology Journal
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    • v.28 no.3
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    • pp.306-310
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    • 2012
  • Populations of plant-parasitic nematodes were evaluated in soil and root samples from areas planted with passion fruit, or covered with adjacent native cerrado vegetation, in five different cropping regions of the Brazilian Federal District. From July 2007 to January 2008, twenty samples had been collected in five Rural Centers (N$\acute{u}$cleo Rural-NR) planted with passion fruit. In five sites, samples were also collected in native cerrado vegetation. Specimens of nine genera of plant-parasitic nematodes (Meloidogyne, Helicotylenchus, Rotylenchulus, Scutellonema, Pratylenchus, Paratylenchus, Hemicycliophora, Xiphinema and Criconemoides) were found in the present survey. Important nematode pathogens, such as Rotylenchulus reniformis and Meloidogyne spp. were observed in the passion fruit plantations throughout five major producing regions of the Brazilian Federal District.

Screening and Histopathological Characterization of Korean Carrot Lines for Resistance to the Root-Knot Nematode Meloidogyne incognita

  • Seo, Yunhee;Park, Jiyeong;Kim, Yong Su;Park, Yong;Kim, Young Ho
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.75-81
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    • 2014
  • In total, 170 carrot lines developed in Korea were screened for resistance to Meloidogyne incognita race 1 to select parental genetic resources useful for the development of nematode-resistant carrot cultivars. Using the gall index (GI), gall formation was examined on carrot roots inoculated with approximately 1,000 second-stage juveniles of the nematode 7 weeks after inoculation. Sixty-one carrot lines were resistant (GI ${\leq}1.0$), while the other 109 were susceptible (GI > 1.0) with coefficient of variance (CV) of GI for total carrot lines 0.68, indicating low-variation of GI within the lines examined. The histopathological responses of two carrot plants from resistant and susceptible lines were examined after nematode infection. In susceptible carrots, giant cells formed with no discernible necrosis around the infecting nematodes. In the resistant carrot line, however, no giant cells formed, although modified cells were observed with extensive formation of necrotic layers through their middle lamella and around the infecting nematodes. This suggested that these structural modifications were related to hypersensitive responses governed by the expression of true resistance genes. Therefore, the Korean carrot lines resistant to the nematode infection are potential genetic resources for the development of quality carrot cultivars resistant to M. incognita race 1.

Efficacy of Soil Amendment with Medicinal Plant Materials for the Control of Root-knot Nematode (Meloidogyne incognita) in Tomato

  • Kim, Young-Ho;Hafeez, U.Khan;Kim, Jung-Ho;Jeon, Yong-Ho;Lee, Eun-Jung;Chang, Sung-Pae
    • The Plant Pathology Journal
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    • v.19 no.3
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    • pp.138-142
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    • 2003
  • Soil amendments with oriental herbal medicines such as fruit of Anethum graveolens, flower buds of Syzygium aromaticum, rhizome of Cnidium officinale, rhizome of Coptis chinensis, root bark of Paeonia suffructicosa, stem bark of phellodendron amurense, and stem bark of Cinnamomum cassia at the rate of 0.2% (weight by volume of soil) significantly reduced Meloidogyne incognita infection (root gall formation) of tomato seedlings compared with the control. The most effective treatments were root bark of p. suffructicosa and stem bark of C. cassia as they gave minimum numbers of galls on tomato roots (4.7% and 8.9%, respectively, relative to control) as compared to other treatments. Another study with root bark of p. suffructicosa and C. cassia at different application doses also showed consistent results in reducing gall number. The control efficacy decreased as the application doses were lower-ed, indicating their dose-dependent control activities. These treatments significantly enhanced aboveground plant growths (total masses).

Responses of Guava Plants to Inoculation with Arbuscular Mycorrhizal Fungi in Soil Infested with Meloidogyne enterolobii

  • Campos, Maryluce Albuquerque Da Silva;Silva, Fabio Sergio Barbosa Da;Yano-Melo, Adriana Mayumi;Melo, Natoniel Franklin De;Pedrosa, Elvira Maria Regis;Maia, Leonor Costa
    • The Plant Pathology Journal
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    • v.29 no.3
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    • pp.242-248
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    • 2013
  • In the Northeast of Brazil, expansion of guava crops has been impaired by Meloidogyne enterolobii that causes root galls, leaf fall and plant death. Considering the fact that arbuscular mycorrhizal Fungi (AMF) improve plant growth giving protection against damages by plant pathogens, this work was carried out to select AMF efficient to increase production of guava seedlings and their tolerance to M. enterolobii. Seedlings of guava were inoculated with 200 spores of Gigaspora albida, Glomus etunicatum or Acaulospora longula and 55 days later with 4,000 eggs of M. enterolobii. The interactions between the AMF and M. enterolobii were assessed by measuring leaf number, aerial dry biomass, $CO_2$ evolution and arbuscular and total mycorrhizal colonization. In general, plant growth was improved by the treatments with A. longula or with G. albida. The presence of the nematode decreased arbuscular colonization and increased general enzymatic activity. Higher dehydrogenase activity occurred with the A. longula treatment and $CO_2$ evolution was higher in the control with the nematode. More spores and higher production of glomalin-related soil proteins were observed in the treatment with G. albida. The numbers of galls, egg masses and eggs were reduced in the presence of A. longula. Inoculation with this fungus benefitted plant growth and decreased nematode reproduction.

Occurrence of Plant-Parasitic Nematodes on Ornamental Foliage Plants, Citrus Orchards, and Tea Plantations in Korea (국내 관엽식물 및 작물재배지의 식물기생선충 발생 조사)

  • Sungchan Huh;Namsook Park;Yongchul Kim;Insoo Choi
    • Research in Plant Disease
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    • v.29 no.4
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    • pp.459-463
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    • 2023
  • This study was conducted to investigate the plant-parasitic nematodes of ornamental foliage plants, citrus orchards, and tea plantations from July to December 2022. As a result of the investigation of plant-parasitic nematodes in 415 foliage plants, root-lesion nematodes were detected most frequently, followed by root-knot nematodes, pin nematodes, and other nematodes. In tea plantations, spiral nematodes, cyst nematodes, and root-knot nematodes were detected. Citrus nematodes, ring nematodes, and root-knot nematodes were discovered in citrus orchards. In foliage plants, tea plantations, and citrus orchards, the detection rate of plant-parasitic nematodes was not high, but root-lesion nematodes were detected. Therefore, it is necessary to apply appropriate control methods to manage root-lesion nematodes during the cultivation of foliage, tea, and citrus plants.

Resistance of Newly Introduced Vegetables to Meloidogyne arenaria and M. incognita in Korea (새로운 채소류의 고구마뿌리혹선충과 땅콩뿌리혹선충에 대한 저항성)

  • Kim, Donggeun;Ryu, Younghyun;Huh, Changseok;Lee, Younsu
    • Research in Plant Disease
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    • v.19 no.4
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    • pp.294-299
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    • 2013
  • To select resistant vegetables against two species of root-knot nematodes, M. incognita and M. arenaria, 39 vegetables belongs to 7 families, 13 genera, 25 species were screened in greenhouse pot test. Susceptible vegetables to both nematodes were amarath and leaf beet in Amaranthaceae, Malabar spinach in Basellaceae, Moroheiya in Tiliaceae, and Water-convolvulus in Convolvulaceae, Pak-choi in Brassica campestris var. chinensis, Tah tasai in B. campestris var. narinosa, B. campestris var. chinensis x narinosa, Leaf mustard, Mustard green in B. juncea, Kyona in B. juncea var. laciniate, Choy sum in B. rapa subsp. arachinenesis, Kairan in B. oleracea var. alboglabra, Arugula in Eruca sativa, Garland chrysanthemum in Chrysanthemum coronarium, Endive in Cichorium endivia, Artichoke in Cynara cardunculus var. scolymus, Lettuce in Lactuca sativa. Resistant to M. arenaria but susceptible to M. incognita were B. oleracea cv. Matjjang kale, B. oleracea var. gongyloides cv. Jeok kohlrabi, and C. intybus cv. Radicchio. Resistant vegetables to both nematodes were C. intybus cv. Sugar loaf, Grumoro, Radichio treviso, B. oleracea cv. Manchu collard, Super matjjang, B. oleracea italica, B. oleracea var. botrytis italiana, and Perilla in Lamiaceae. Vegetables resistant to both species of root-knot nematodes could be used as high-valued rotation crops in greenhouses where root-knot nematodes are problem.

The Effect of Anaerobic Fermentation Treatment of Wheat bran on the Root-Knot Nematodes and the Quality of Melons in Plastic Film House Soil (밀기울 토양 혐기발효 처리가 멜론의 뿌리혹선충 방제 및 품질에 미치는 영향)

  • Park, Dong-Kum;Kim, Hong-Lim;Park, Kyoung-Sub;Huh, Yun-Chan;Lee, Woo-Moon;Lee, Hee-Ju
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.311-316
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    • 2010
  • This study was conducted to investigate the effect of anaerobic fermentation of wheat bran to prevent root-knot nematodes which are infected in plastic house due to over 10 years continuous cultivation of fruits and vegetables. Anaerobic fermentation treatment of wheat bran was done for 20 days by mixture of 2,000 kg fresh wheat bran per 10 are and soil with water in 30 cm soil depth. Chemical treatment of fosthiazate was done by mixture of 6 kg soil for 7 days. Both treatments show suppression of density of rootknot nematodes, especially in anaerobic fermentation treatment. Anaerobic fermentation treatment keeps the low level of root-knot nematode density until 90 days of cultivation and also showed good effect of melon growth. Related with death percentage of melon plant, anaerobic fermentation treatment shows only 3% and also large size of quality fruit but control 65%. Anaerobic fermentation treatment of wheat bran have proved to control the level of root-knot nematodes instead of synthetic chemicals for at least one cropping season and it showed good effect to fruit quality.

Effects of Treatment Time of Cadusafos and Fosthiazate for the Control of Meloidogyne arenaria on Oriental Melon (카두사포스와 포스치아제이트 처리시기에 따른 땅콩뿌리혹선충 방제효과)

  • 김동근;김진배;이재국;최성국;윤재탁
    • Korean journal of applied entomology
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    • v.41 no.4
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    • pp.293-298
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    • 2002
  • Cadusafos and fosthiazate were applied at the rate of 1.8 g a.i./6$\m^2$ as pre-plant or pre-plant+post-plant treatments on winter-grown oriental melon in a greenhouse soil infested with Meloidogyne arenaria. Nematicides reduced root-knot nematode population from 35 to 90% compared with control; fosthiazate was better than cadusafos (P = 0.003) and fosthiazate pre-plant+post-plant application reduced nematode population densities as much as 90%. Nematicides increased yield in an average of 23% (11-38%) in May, 39% (2-65%) in June, and 31% (12-46%) for the total (P = 0.085). The residue in the fruit of oriental melon by post-plant treatment of fosthiazate exceeded maximum residue limit of 0.2ppm, while the Cadusafos residue was below the limit. It is concluded that fosthiazate cannot be used as a post-plant treatment in winer-grown oriental melon with overall consideration, i. e., danger of residue, value of melon, costs of nematicide, consumer demand on safer agricultural products, and effects of fallow.

The Effect Control of Root-knot Nematode by Using Rapeseed Meal in Continuous Cultivation at Greenhouse (유채박 이용 시설하우스 연작재배 시 뿌리혹선충 밀도억제효과)

  • Lee, Hoo-Kwan;Lee, Young-Hwa;Kim, Kwang-Soo;Jang, Young-Seok;Choi, In-Hu
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.93-100
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    • 2015
  • The objective of this study was to determine the effectiveness of rapeseed meal in controlling soil nematode. Two different rapeseed meals (Jeju local rape varieties and 'Sunmang' variety) were mixed with the soil to control nematodes environmentally. When soil physical properties in the rapeseed meal-mixed soils were analyzed, OM (organic matter), $P_2O_5$, Ca, Mg, CEC (Cation Exchange Capacity) value increased. Especially, the level of OM was 3-fold higher than control soil. Glucosinolate content of rapeseed meal was higher in Jeju local rape varieties than 'Sunmang' variety. The major components of glucosinolates were consisted of progoitrin, gluconapin, glucobrassiaca napin, and sinigrin. These components were likely to be involved in reducing nematode density.