• 한국유기농업학회지
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• 제19권spc호
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• pp.267-270
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• 2011

Root-knot nematodes cause a significant damage on fruit yield and quality of green house growing crops. To asses the effect of eggplant rootstock, Torvum vigor', TaibyouVF' and 'Daitaro' were grafted on eggplants(Solanum melongena cv. Chookyang) and planted in root-knot nematode infested microplot in green house and compared their fruit yield, quality and plant growth with non-grafted control. Eggplant grafted with Torvum vigor had the highest fruit yield and top growth and followed by Daitaro. Non-grafted eggplant had lower yield but had higher root weight because of heavy root-knot nematode infection. Rootstock grafting in eggplant farming is a good alternative technique in root-knot nematode infested green houses without compromising fruit yield and can be applied instantly as organic farming practice.

• 식물병연구
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• 제14권1호
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• pp.76-78
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• 2008

포항지역의 부추 연작 장해를 조사하는 중 부추에서는 처음으로 뿌리혹선충의 기생이 발견하여 보고한다. 부추에 기생하는 뿌리혹선충은 Meloidogyne incognita로 동정되었다. 연작 장해지에는 뿌리혹선충, 뿌리응애(Rhizoglyphus sp.), 시들음병(Fusarium oxysporum f. sp. cepae)등이 복합적으로 발생되고 있었지만 발생 빈도와 피해 정도를 보아 연작장해의 주요 원인은 뿌리혹선충이었다.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.227-232
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• 1994

In order to microbially control root-knot nematode(Meloidogyne incognita) in tomato, a strain BtTH109 of Bacillus thuringiensis producing root-knot nematocidal toxin was isolated. The strain BtTH109 was identified B. thuringiensis subsp. indiana(serotype 16) based on flagella antigenicity, biochemical properties, and morphological charcateristics. The strain BtTH109 have extracellularly produced a root-knot nematocidal toxin, which was very toxic against not only egghatch but also the 2nd-nematode larva of root-knot nematode in vitro.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.133-138
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• 2003

Five seed kernel namely, Neem (Azadirachta indica A. Juss.), Pongamia (Pongamia glabra L. Pierre), Tamarind (Tamarindus indica L.), Mahua (Madhuca indica Gmel.) and Shikakai (Acacia cancinna De.) were tested against hatching of eggs and larval mortality of Meloidogyne incognita causing root knot disease in mulberry along with Furadan (Carbofuran) and Bionema (a bioformulation developed from Verticillium chlamydosporium) for comparison. Results revealed that highest hush-up of hatching was observed in Neem (77.40%) and Pongamia (75.99%) seed kernel extracts at 100% concentration over the check. Similarly, highest larval mortality was observed in Neem and Pongamia by 76.00% and 74.50%, respectively at 100% concentration after 72 hrs of exposure period. Pot culture studies revealed that pre-application of seed kernel powders (20 days before inoculation of nematode) found to be more effective in controlling the root knot disease than post application. In pre application of seed kernel powders, maximum reduction of root knots was observed in case of Neem seed kernel powder (54.85%) followed by Pongamia (51.9%). Similar trend was also observed in reduction of egg masses/plant and nematode population ／250 cc soil. Rest of the seed kernel extracts was found to be less effective in suppression of hatching, enhancing the larval mortality and controlling the root knot disease. However, application of Furadan and Bionema tested for the comparison were found to be more effective than seed kernel powders. The generated information seems to be useful in developing an ecofriendly integrated approach for the control of root knot nematode disease in mulberry.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.175-179
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• 2004

Trichoderma harzianum-THN1 parasitising the egg masses of root knot nematode Meloidogyne incognita was isolated from galled mulberry roots and evaluated for its potential to control root knot disease. In pot experiments root galling was reduced and leaf yield increased significantly following soil treatment with T. harzianum-THN1. The extracts obtained from the soils inoculated with T. harzianum-THN1 drastically inhibited the hatching of nematode eggs and the effect was irreversible even after the eggs were transferred to fresh water. The fungus was equally effective in controlling the disease in nematode infested mulberry garden under field conditions which was significant over the most commonly used egg parasitic fungus Paecilomyces lilacinus. The disease reduction recorded with T. harzianum was on par with the plants treated with the nematicide Carbofuran. The results suggest that T. harzianum- THN1 could be used as a potent ecofriendly biocontrol agent against M. incognita in mulberry without any residual toxicity to silkworms. T. harzianum- THN1 can form an important component of integrated disease management package in mulberry cultivation.

• 한국미생물·생명공학회지
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• 제22권6호
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• pp.679-684
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• 1994

For a biological control of root-knot nematode (Meloidogyne incognita) in tomato, efficiency of Bacillus thuringiensis subsp. indiana strain TH109 (BtTH109) on the nematode control was investigated. After the mixture of strain BtTH109 and wheat bran was treated into rhizosphere of the tomato plants with nematode eggs, the stem height and root growth of plants increased. And the juveniles and eggs of nematode are not found in both roots of tomatoes and pot soil after cultured broth of the strain BtTH109 treated 4 times at 3 day-interval into rhizosphere of the infected tomatoes.

• The Plant Pathology Journal
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• 제25권2호
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• pp.189-192
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• 2009

The effect of plant extracts of eucalyptus (Eucalyptus chamadulonsis), garlic (Allium sativium), marigold (Tagetes erecta) and neem (Azadirachta indica) and essential oils were tested on the suppression of root-knot nematode Meloidogyne incognita under greenhouse and field conditions. In vitro study, all tested treatments had nematicidal effect on nematode juveniles after 24 and 48 hours from exposures. The highest percentage of nematode mortality was achieved by application of neem extract (65.4%), essential oils (64.4%) and marigold extract (60.5%), followed by garlic and eucalyptus extracts (38.7-39.5%). Under greenhouse and field conditions, neem extract and essential oils treatments were more effective in reducing population numbers of the M. incognita in soil and root gall index compared to other treatments. In field experiments, the maximum protection of tomato plant against root-knot nematode was obtained by application of neem and essential oil treatments, 44.2 and 32.6%, respectively.

• 식물병연구
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• 제18권1호
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• pp.29-34
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• 2012

흰가루병과 뿌리혹선충병은 박과작물에 발생하는 매우 중요한 병이다. 본 실험은 시판된 국내 주요 박과작물(오이 21품종, 수박 9품종, 참외 7품종, 멜론 2품종)을 이용하여 흰가루병과 뿌리혹선충병에 대한 저항성을 평가하기 위하여 수행하였다. 흰가루병 조사는 정식 60일 후 조사하였다. 21개의 오이 품종 중 '강력삼척'과 '선호백침 다다기'는 중도저항성을 나타냈고 나머지 오이 품종은 모두 감수성이었다. 수박 품종은 모두 감수성을 나타냈다. 참외 품종에서는 저항성을 나타내는 품종은 없었으나 'PMR터보'와 'PMR빅토리' 2개 멜론 품종은 저항성을 나타냈다. 뿌리혹선충병은 유묘에 접종 45일 후 병 발생을 조사하였다. 오이 품종 '백봉다다기'가 중도저항성을 나타냈고 다른 20개의 오이 품종은 모두 감수성이었다. 수박 품종은 '달고나꿀'이 저항성이었으며 다른 품종은 중도저항성이거나 감수성이었고, 조사된 모든 참외와 멜론 품종은 뿌리혹선충병에 대하여 감수성이었다. 이상의 결과를 근거로 흰가루병 및 뿌리혹선충에 저항성인 품종은 박과작물의 유기재배에 이용할 수 있을 것이다.

• Natural Product Sciences
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• 제24권4호
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• pp.272-283
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• 2018

The root-knot nematode, Meloidogyne incognita caused a serious damage to many plants. The phenolic components of the leaves of Schinus terebinthifolius were investigated as potential nematicidal agents for M. incognita. Nine compounds were isolated and characterized as viz., 1,2,3,4,6-pentagalloyl glucose (1), kaempferol-3-O-${\alpha}$-L-rhamnoside (Afzelin) (2), quercetin-3-O-${\alpha}$-L-rhamnoside (Quercetrin) (3), myricetin (4), myricetin-3-O-${\alpha}$-L-rhamnoside (Myricetrin) (5), methylgallate (6), protocatechuic acid (7), quercetin (8), and gallic acid (9) using nuclear magnetic resonance (NMR) spectroscopy. Compound 1 showed pronounced nematicidal activity compared to Oxamyl as a positive control. It showed the lowest eggs-hatchability (34%) and the highest mortality in nematode population (21% after 72 hours of treatment) at a concentration of $200{\mu}g/mL$. It exhibited the best suppressed total nematode population, root galling and number of eggmasses in infected tomato plants. The total carbohydrates and proteins were also significantly induced by 1 with reduction in total phenolics and increase in defense-related proteins. Thus, compound 1 could be a promising, more safe and effective natural nematicidal agent for the control of root-knot nematodes.

• The Plant Pathology Journal
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• 제25권3호
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• pp.247-255
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• 2009

Root-knot nematode species, such as Meloidogyne hapla, M. incognita, M. arenaria, and M. javanica are the most economically notorious nematode pests, causing serious damage to a variety of crops throughout the world. In this study, DNA sequence analyses were performed on the D3 expansion segment of the 28S gene in the ribosomal DNA in an effort to characterize genetic variations in the three Meloidogyne species obtained from Korea and four species from the United States. Further, PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism), SCAR (Sequence Characterized Amplified Region) PCR and RAPD (Randomly Amplified Polymorphic DNA) were also utilized to develop methods for the accurate and rapid species identification of the root-knot nematode species. In the sequence analysis of the D3 expansion segment, only a few nucleotide sequence variations were detected among M. incognita, M. arenaria, and M, javanica, but not M. hapla. As a result of our haplotype analysis, haplotype 5 was shown to be common in M. arenaria, M. incognita, M. javanica, but not in the facultatively parthenogenetic species, M. hapla. PCR-RFLP analysis involving the amplification of the mitochondrial COII and large ribosomal RNA (lrRNA) regions yielded one distinct amplicon for M. hapla at 500 bp, thereby enabling us to distinguish M. hapla from M. incognita, M. arenaria, and M. javanica reproduced via obligate mitotic parthenogenesis. SCAR markers were used to successfully identify the four tested root-knot nematode species. Furthermore, newly attempted RAPD primers for some available root-knot nematodes also provided some species-specific amplification patterns that could also be used to distinguish among root-knot nematode species for quarantine purposes.