• Korean Journal of Pharmacognosy
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• v.5 no.2
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• pp.111-124
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• 1974

The radioactive compound sodium $acetate-U-C^{14}\;(C^{14}-acetate)$ was administered to two- and four-year-old July and September American ginseng (Araliaceae, Panax quinquefolium L.) plants and cuttings. The $C^{14}-acetate$ uptake was approximately 99%. The autoradiochromatograms suggest that the saponins isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration (% plant dry weight) of semi-purified saponins were high in the leaves (13.8%), as compared to fruits (9.8%), stems (7.9%) and roots (6.3%). The average percentage of $C^{14}-acetate$ incorporation into panaquilins was 4.8%. The average percentage of $C^{14}-acetate$ incorporation into panaquilins B and C was higher (1.40% and 1.13%, respectively) than that into panaquilins C, (d), G-1 and G-2 (0.75%, 0.65%, 0.13% and 0.53%, respectively). Panaquilin synthesis may be depending upon the part, collection period and age of the plant. The average percentage of $C^{14}-acetate$ incorporation into panaquilin B is high in roots (0.58%) and stems (0.48%); that into panaquilins C and (d) high in leaves (0.40% and 0.45%, respectively); and that into panaquilin E high in roots and leaves (0.55% and 0.50%, respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-1). Panaquilins B, C and G-1 may be biosynthesized more actively in four-year-old plants and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-1 may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and cailus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that $C^{14}-acetate$ was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act. 0.56 mmcCi/mg) and four-year-old plants $(sp.\;act.\;0.54\;m{\mu}Ci/mg)$.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.323-332
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• 2003

This study was carried out to provide information for the present status of soil pollution near abandoned old-zinc mining area through analysis of bound form and 0.1 N-HCl extractable concentrations of heavy metals in soils and plants. Feasibility of endemic plants for phytoremediation was evaluated by the investigation of vegetation in soils. Cd contents of the selected samples near old-zinc mining soils ranged from 0.2 to $42mg\;kg^{-1}$. Nonagricultural soils near the mining area contained great amounts of Zn, Pb, Cd, and Cu than the paddy and upland soils. Some Korean wild plants, Artemisia princeps, Artemisia montana, Erigeron canadensis, and Pueraria thunbergiana, were found to grow vigorously in the studied area. Among them, Artemisia princeps was selected as a possible phytoremediator for cleaning heavy metal contaminated soils. Artemisia princeps contained about 43 and $52mg\;kg^{-1}$ of Cd in their root and shoot as dry weight, respectively. Average contents of Cd in the rhizosphere soil, $15.68mg\;kg^{-1}$, was slightly higher than the soil-root interface soils, $14.1mg\;kg^{-1}$. Sequential extraction of Cd contaminated soils showed that average $2.4mg\;kg^{-1}$ (about 7%) of cadmium existed as exchangeable form and the average amounts increased as follows : adsorbed < organically bound < exchangeable << oxide carbonate << sulfide residual fractions. Amendment of organic by-product fertilizer in metal-contaminated soils promoted the growth of roots significantly as compared with the other treatments containing chemical fertilizer.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.335-342
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• 2010

Sulforaphane (SFN) and total phenolics (TPC) contents and antioxidant activity (AA) were analyzed from 13 radish genotypes (Rhaphanus sativus L.), cultivated at 3 locations with different altitudes (Gangneung: asl 5 m, Jinbu: asl 550 m, and Daegwallyeong: asl 750 m). SFN varied greatly from 0.1 to $120.5{\mu}g{\cdot}g^{-1}$ in dry weight test and was significantly affected by location ($P{\leq}0.001$), genotype ($P{\leq}0.001$) and $location{\times}genotype$ interaction ($P{\leq}0.01$). Radishes, cultivated at Daegwallyeong site, showed higher SFN than those of other locations. Among different genotypes, the root of 'Black radish' and leaves of 'Purunmu' of Daegwallyeong had the highest SFN (107.8 and $120.5{\mu}g{\cdot}g^{-1}$, respectively). TPC in root was affected by genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.01$), but not by location. In leaves, TPC was affected by location ($P{\leq}0.01$), genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.001$). AA expressed as electron donating ability was significantly influenced by location, genotype and $location{\times}genotype$ interaction and correlated positively with TPC ($Pearson's$ $r$=0.897) in root. These results suggest that radish could be a good source of functional food and high altitude location such as Daegwallyeong has potential for the production of radish with high content of health promoting factors.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.39-46
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• 2015

Objective of this research was to investigate the influence of compositions and concentrations of fertilizer solutions on the vegetative growth and nutrient uptake of 'Seolhyang' strawberry. To achieve this, the solutions of acid fertilizer (AF), neutral fertilizer (NF), and basic fertilizer (BF) were prepared at concentrations of 100 or $200mg{\cdot}L^{-1}$ based on N and applied during the 100 days after transplanting. The changes in chemical properties of the soil solution were analysed every two weeks, and crop growth measurements as well as tissue analyses for mineral contents were conducted 100 days after fertilization. The growth was the highest in the treatments with BF, followed by those with NF and AF. The heaviest fresh and dry weights among treatments were 151.3 and 37.8 g, respectively, with BF $200mg{\cdot}L^{-1}$. In terms of tissue nutrient contents, the highest N, P and Na contents, of 3.08, 0.54, and 0.10%, respectively, were observed in the treatment with NF $200mg{\cdot}L^{-1}$. The highest K content was 2.83%, in the treatment with AF $200mg{\cdot}L^{-1}$, while the highest Ca and Mg were 0.98 and 0.42%, respectively, in BF $100mg{\cdot}L^{-1}$. The AF treatments had higher tissue Fe, Mn, Zn, and Cu contents compared to those of NF or BF when fertilizer concentrations were controlled to equal. During the 100 days after fertilization, the highest and lowest pH in soil solution of root media among all treatments tested were 6.67 in BF $100mg{\cdot}L^{-1}$ and 4.69 in AF $200mg{\cdot}L^{-1}$, respectively. The highest and lowest ECs were $5.132dS{\cdot}m^{-1}$ in BF $200mg{\cdot}L^{-1}$ and $1.448dS{\cdot}m^{-1}$ in BF $100mg{\cdot}L^{-1}$, respectively. For the concentrations of macronutrients in the soil solution of root media, the AF $200mg{\cdot}L^{-1}$ treatment gave the highest $NH_4$ concentrations followed by NF $200mg{\cdot}L^{-1}$ and AF $100mg{\cdot}L^{-1}$. The K concentrations in all treatments rose gradually after day 42 in all treatments. When fertilizer concentrations were controlled to equal, the highest Ca and Mg concentrations were observed in AF followed by NF and BF until day 84 in fertilization. The BF treatments produced the highest $NO_3$ concentrations, followed by NF and AF. The trends in the change of $PO_4$ concentration were similar in all treatments. The $SO_4$ concentrations were higher in treatments with AF than those with NF or BF until day 70 in fertilization. These results indicate that compositions of fertilizer solution should to be modified to contain more alkali nutrients when 'Seolhyang' strawberry is cultivated through inert media and nutri-culture systems.

• Korean Journal of Organic Agriculture
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• v.16 no.4
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• pp.435-453
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• 2008

The crop rotation is very important in the organic agriculture and the soybean crop should be included in the rotation. Even though the inoculation of the root can be performed by the soil inoculation in the soybean crop, the effect of the soil inoculation should be proved in the organic soil. For the research of the effects in the formation of the nodule of soybeans, the growth and the quantity through the inoculation into the soil, the field experiment was conducted in 2006 at Ipjang Experimental Farm of Dankook University. The number of nodule in the testing varieties of the inoculated soil plot was $1.4{\sim}2$ times larger than those in the non-inoculated soil plot. The number of nodule was decreased in the non-inoculated soil plot after R2 stage, but it was increased in the inoculated before R4 stage and it was decreased after then. The periodical change of weight of the nodule was shown as same as the number of nodule. The dry weight of inoculated was increased than non-inoculated in Daewonkong, but it was decreased than non-inoculated in Shinpaldalkong2 and SS2-2. The amount of chlorophyll of the inoculated soil plot was more than the non-inoculated soil plot by 9% in Daewonkong, but the inoculated soil plot was less than the non-inoculated soil plot by 13%, 33% each in Shinpaldalkong2 and SS2-2. The number of pods, the number of grains and the weight of grains in the yield characteristics of the inoculated soil plot were more than in the non-inoculated soil plot in Daewonkong, but the number of pods, grains and the weight of grains in the yield result in the inoculated soil plot was less than in the non-inoculated soil plot in Shinpaldalkong2 and SS2-2. The mean yield(per 10a) of Daewonkong was increased 11% in the inoculated soil plot, but the mean yeil(per 10a) of Shinpaldalkong2 was decreased 4% and SS2-2 was also decreased 23%.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.3
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• pp.145-152
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• 2013

This study aimed to investigate the growth and physiological characters of Liriodendron tulipifera seedlings in responses to two different levels of elevated air temperature and $CO_2$ concentration. The seedlings were grown in environment-controlled growth chambers with two combinations of air temperature and $CO_2$ conditions: (1) $22^{\circ}C$ + ambient $CO_2$ $380{\mu}mol\;mol^{-1}$ and (2) $27^{\circ}C$ + $770{\mu}mol\;mol^{-1}$. Physiological characters such as growth, photosynthesis, and water use efficiency, were monitored for 85 days. The seedlings under the elevated treatment showed a greater amount of growth in tree height, compared with those under the control. Regarding the characteristics of assimilatory organs, the elevated treatment resulted in a greater amount of total leaf area, leaf unfolding, and dry weight per leaf area. No significant differences were found in photosynthesis capacity between the two treatments. The increase in water use efficiency with increased intercellular $CO_2$ partial pressure appeared overall lower in the seedling under the elevated treatment, compared with those under the control. The total leaf area of the seedlings under the elevated treatment was larger than that under the control, indicating a higher amount of photosynthesis. In addition, an increase of root growth was noted under the elevated treatment. A resistance mechanism of water stress may be attributed to a higher amount of organ growth as well as the tree height under the elevated treatment than the control.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.78-85
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• 2016

This study was carried out to determine the effects of shading and leaf mold treatment on growth characteristics and photosynthesis responses of Peucedanum japonicum in forest farming. It is very valuable as a sort of health food, so that the demand for the vegetable has increased recently. The experiment can not only increase the yield but also contribute to the development of eco-friendly technology for high-quality P. japonicum. It was performed by shading treatments (full sunlight, 35%, 50% and 75% shading) and leaf mold treatments (control, pine tree and chestnut tree). Height, stem diameter, root collar diameter, number of stem and dry weight were the highest in chestnut-leaf mold under full sunlight. Leaf area, leaf length and leaf width were the highest in chestnut-leaf mold under 35% shading. Photosynthetic rate, conductance to $H_2O$, transpiration rate and water use efficiency were the highest in chestnut-leaf mold under full sunlight. Specially, photosynthetic rate was higher under chestnut-leaf mold in all shading treatment, and getting lower in the higher shading rate. As a result of surveying the whole experiment, it is concluded that P. japonicum grows nicely by maintaining 35% shading under chestnutleaf mold in forest farming. Thus, it is the most effective way to increase the yield for high-quality P. japonicum with eco-friendly technology.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.165-169
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• 2001

Seed coat of sword bean (Canavalia gladiata) is very thick and hard, it is difficult to absorb water during germination and it requires much time that cotyledon come out from seed coat since seminal root appearanced. Therefore this experiment was carried out to increase the germination rate by easing water absorption through mechanical scarification on seed coat. Non-scarification seed did not germinated at 7 days after treatment but scarification seed showed germination rate of 98%. Therefore mechanical scarification of seed coat was increased germination rate. Non-scarification seed absorbed less than 10% water of dry weight at 8 hours after treatment but scarification seed absorbed about 90% water at 7 hours after treatment and more than 150% water at 8 hours and swelled about 1.4-1.5 times in length and width of seed. Germination rate of scarification seed was high under high temperature and it was 96% and 93% at $25^{\circ}C$ and 3$0^{\circ}C$, respectively. The rate of cotyledon emergence on clay loam soil was the highest among bed soils. Soaked seed after mechanical scarification in distilled water was germinated faster than non-soaked seed on early in bed soil. The rate of cotyledon emergence was more than 92% at 9 days after sowing but non-scarification and soaked seed was germinated late and showed very low germination rate as 67%.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.46-50
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• 2013

Some rhizobacteria producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase can make plant to continue growth under the stress conditions through lowering the level of phytohormone, ethylene which inhibits the plant growth and accelerates plant aging. In this study, some rhizobacteria producing ACC deaminase have been isolated from the rhizosphere of plants grown at sand beaches, and identified as Escherichia hermannii m-2, Enterobacter asburiae m-4, Pseudomonas thivervalensis BD2-26 and Pseudomonas brassicacearum subsp. neoaurantiaca BD3-35 through sequencing of 16S rRNA genes. Strain BD3-35 showed the highest activity of ACC deaminase among the isolates, 20.26 ${\alpha}$-ketobutyrate ${\mu}M/mg$ protein/h. Strains BD3-35 and BD2-26 secreted a phytohormone cytokinin, and strains m-4 and m-2 could produce auxin and abscisic acid, respectively. When these bacteria were applied to the 7-day old tomato plant under drought stress for 7 days, strains BD3-35, m-2, and m-4 increased the length of tomato root by 14, 15, and 35%, respectively, and strains m-2, BD2-26 and BD3-35 increased the dry weight of tomato plant by 22, 33, and 68%, respectively compared to the uninoculated control tomatoes. Therefore, these rhizobacteria may be utilized as a microbial fertilizer for the plants under drought stress.

• Journal of Environmental Science International
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• v.22 no.1
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• pp.17-23
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• 2013

In order to evaluate the effect of various organic soil conditioner ratios on the soil moisture content and growth of Cotoneaster horizontalis, a container type for wall-planting experiment was conducted in a greenhouse at Konkuk University under non-irrigation. The experimental planting grounds were prepared with different organic soil conditioner ratios ($A_1L_0$, $A_8L_1$, $A_4L_1$ $A_2L_1$ and $A_1L_1$), and a drought-tolerant ornamental variety of Cotoneaster horizontalis was planted. The change in soil moisture content, plant height, number of branches, number of dead leaves, number of leaves, number of shoots, length of node, length of leaf, width of leaf, root-collar caliper, chlorophyll content, and survival rate were investigated, from April to Jun 2010. The results of soil moisture content measurements were analyzed with weight units in the container type for wall-planting during the dry summer season. The soil moisture contents were significantly enhanced in the container type for wall-planting in increasing order as the amount of soil conditioner level was increased ($A_1L_1$ > $A_2L_1$ > $A_4L_1$ > $A_8L_1$ > $A_1L_0$). Compared to the control treatment application (amended soil with 100% + organic soil conditioner 0%), the highest plant growth was observed in the treatment of $A_1L_1$ application (amended soil with 50% + organic soil conditioner 50%). However, the differences between the organic soil conditioner ratio treatments of $A_1L_1$, $A_4L_1$, and $A_8L_1$ organic soil conditioner application were mostly not significant. The survival rate increased with the increasing application of organic soil conditioner, but in the control treatment application all the plants died. The experimental results from clearly demonstrated that the organic soil conditioner improved the survival rate more than the growth of Cotoneaster horizontalis. Therefore, Cotoneaster horizontalis is expected to be a highly valuable shrub for green wall systems, when considered for us in integration system or for increasing soil water contents in planting grounds.