• 식물조직배양학회지
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• 제25권2호
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• pp.75-80
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• 1998

부정근발생에 관한 도립 및 직립배양의 영향을 구명하기 위하여 6일간 자란 팥의 유경 절편을 사용하였다. $2\times10$^{-4}M$ IAA 용액을 48시간 처리할 때에 절편의 위치를 직립 또는 도립으로 배양 후 96시간 직립으로 배양하였다. 48시간 처리시 직립배양하면 부정근의 형성은 형태학적 기부에서만 발생하지만, 도립 전처리 배양을 하면 유경절편의 양끝에서 부정근이 발생하였다. IAA 전처리는 절편의 방향과 관계없이 부정근 형성을 촉진시켰다. 부정근 발생시기에 유경절편내의 생화학적 변화를 구명하기 위하여 직립배양 및 도립배양을 한 후 부정근 발생 단계별로 24시간, 48시간, 148시간 후 절편을 상부, 중부, 하부로 등분하여 부정근 발생에 수반되는 효소활성, 절편의 길이 신장 및 생중량을 측정하였다. 절편의 길이 신장은 절편의 상부에서 높게 나타났고 생중량은 발근부위에서 높게 나타났다. peroxidase을 catalase 활성은 부정근원기의 형성단계인 24시간에 절편의 발근부위에서 높게 나타났고 이에 비해 IAA oxidase는 양쪽 기부에서 낮았다. 그리고 IAA oxidase와 peroxidase 활성은 48시간 배양인 부정근의 형성동안 발근 부위에서 감소하였다. 도립전처리 유경 절편에 따라서 효소활성의 변화가 일어났으며 peroxidase의 활성은 부정근원기형성과 밀접한 관계를 나타내었다. 따라서 생화학적인 변화는 유경의 발근영역에서 IAA의 수준의 변화와 밀접하게 관련되어 특정부위에서의 부정근 형성을 유도하는 것으로 판단된 것이다.

• 한국환경농학회지
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• 제38권3호
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• pp.154-158
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• 2019

BACKGROUND: 'Shiranuhi' mandarin is a major cultivar among all late ripening type of citrus, and is widely cultivated in Korea. However, many farmers have reported scion root problems in their orchard resulting in reduced flowering and fruiting. It is necessary that the physiology of scion-rooted 'Shiranuhi' mandarin trees is further understood. METHODS AND RESULTS: This experiment was conducted to understand the growth response and physiology of scion-rooted 'Shiranuhi' mandarin hybrids. In our study, 'Shiranuhi' mandarin trees were divided into two groups: trees without scion roots (control) and trees with scion roots. The experiment was conducted in Seogwipo of Jeju, with ten replicates for each group. Growth of trees with scion roots was more vigorous and the trees were taller than the controls. Tree height and trunk diameter of scion-rooted trees were significantly higher than those of control trees. Exposed length of rootstocks of scion-rooted trees was significantly lower (by about 2 cm) than that of control trees (8.6 cm). In terms of root nutrition, carbon contents of scion-rooted trees was significantly lower than that of control trees, but nitrogen and potassium concentrations in scion roots were significantly higher than those in control roots. CONCLUSION: Based on the results, we infer that growth of scion-rooted trees was very vigorous and the content of nitrogen in these roots was higher than that in the control tree roots. Thus, the carbon/nitrogen ratio of scion roots was significantly lower than that of the control roots.

• 생물환경조절학회지
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• 제25권2호
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• pp.100-105
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• 2016

양파 정식기 작업에 적합한 상토 개발을 위해 원예용 유기질 주재료(피트모스, 코이어)의 적합성을 조사했다. 유기질 재료를 무기질 재료(황토 등)와 혼합하여, 묘 출현율, 생육 및 기계정식 적응 특성 등을 조사하였다. 조사 결과 피트모스 혼합 처리구, 코이어 혼합 처리구 모두 묘 출현율, 생육 및 상토 용적밀도에서 유사했으나, 피트모스 혼합 처리구가 코이어 혼합 처리구에 비해 더 높은 상토 응집성을 보였다. 피트모스 혼합 상토의 높은 응집성은 양파 묘 정식 시 뿌리부 무게 증가 등의 효과를 가져와서 정식기 작업 효율성을 증진시키는데 도움을 줄 수 있을 것이라고 생각된다. 이러한 이유로, 양파 정식기 작업에 적합한 상토 개발 시 피트모스를 유기질 주재료로 선정하는 것이 적합하다고 최종 판단하였다.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.614-622
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• 1995

Root caries is very frequently developed on exposed root surface after periodontal surgical treatment. In order to determine the anti-caries effect of Nd : YAG laser irradiation on periodontally exposed root surface, 40 mandibular molar teeth that had been extracted due to excessive periodontal destruction were used as the experimental teeth. All teeth were treated by the same procedure as conventional periodontal root treatment, ie thorough scaling, root planing and root conditioning with tetracycline HCl(100mg/ml, 5min.). Within middle one third of root, mesial half surface(20) or distal half surface(20) was randomly irradiated at various power of 1.0W, 2.0W, 3.0W and 4.0W for 60 seconds by non-contact(5mm) delivery of a pulsed Nd : YAG laser(EN.EL.EN060, Italy). The microhardness was measured by Vikers microhardness tester(Wilson, USA) at 2mm/second of jog speed under 100gm load. The difference of microhardness between irradiated side and non-irradiated side was statistically analyzed ANOVA and Duncan's method. Following results were obtained ; 1. The microhardness(Knoop hardness number) was significantly higher in laser irradiated surface than non-irradiated surface(p<0.05). 2. There was no significant difference in microhardness between experimental groups classified by different laser power(p>0.1). The results suggest that Nd : YAG laser irradiation on exposed root suface after periodontal therapy may inhibit the root caries development by enhancing surface microhardness.

• The Plant Pathology Journal
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• 제17권3호
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• pp.159-163
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• 2001

Influence of soil temperature [accumulated degree-day for the base temperature $5^{\circ}$($\textrm{DD}_5$)] on the development of Meloidogyne arenaria were studied in a winter grown oriental melon greenhouse in Seongju, Korea. Egg masses were first observed on roots at the accumulation of 565 $\textrm{DD}_5$(40 days after transplanting), suggesting that the nematode has completed the first generation in 40 days. Second-stage juveniles (J2) densities were lowest at 863 $\textrm{DD}_5$ in April, first increased at 1,334 $\textrm{DD}_5$ in May, peaked at 2,951 $\textrm{DD}_5$ in July, and decliner thereafter. Development of egg masses and J2 density in soil revealed that M. arenaria could develop in 7-8 generations in a year in the greenhouse. Degree-day monitoring, therefore, could aid to predict nematode development in soil and can be valuable tool a to develop root-knot nematode control strategies.

• 식물병연구
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• 제16권1호
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• pp.10-20
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• 2010

Root-knot nematodes cause billions of dollars in crop losses annually have a broad range of host over 2,000 species of plants. These nematodes are known as obligate, sedentary endo-parasites in a plant host to feed upon to complete their life cycle. To prevent the plant parasitic nematode, methyl bromide was widely applied as a soil fumigant. Other strategies to prevent or control nematodes involve RNAi-mediated suppression, R gene transformation, natural products or chemical treatments, the expression of peptide or proteins in susceptible plants, and others. Over the last decade, the entry in GenBank for Meloidogyne reveals 73,340 ESTs and recently two complete Meloidogyne spp. genomes sequences have simultaneously been presented by two groups. Recent works have demonstrated the effect of RNAi suppression to nematode target genes. These results will provide novel members of genes as a foundation for studies focused on understanding the function of M. incognita nematode genes as well as for the development of novel target genes for parasite control. Thus the successful development of biotechnology-derived plants with nematode resistance will result in large yield benefits for producers as well as environmental benefits and will accelerate the research related to pathogensresistant crops.

• The Plant Pathology Journal
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• 제18권3호
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• pp.142-146
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• 2002

The effect of potassium phosphonate ($KH_2PO_3 or $K_2 HPO_3$) on the control of Phytophthora root rot of lettuce was evaluated in a liquid hydroponic culture. Phosphonate 100 ppm strongly inhibited mycelial growth of Phytophthora species in vitro but did not affect normal growth of lettuce in a greenhouse test. Application of the chemical before infection showed over 94% control value, while it was less than 35% when applied after infection. In a field trial, phosphonate 100 ppm, which was directly supplemented into the nutrient solution, satisfactorily controlled the disease as it did not develop until 28 days after transplanting and remained at less than 2% infection rate at the end of cultivation. Meanwhile, in the control plot, the disease initiated at 7 days after transplanting and developed rapidly reaching over 70% infection rate at 28 days. Population density of the causal pathogen, R drechsleri, in a heavily infested farm was 22.0-25.0 cfu/100 ml of nutrient solution. However, it decreased to 1.3-2.0 cfu/100 ml at 7 days after treatment with phosphonate 200 ppm.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2007년도 춘계학술논문 발표대회
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• pp.13-17
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• 2007

The green roof industry have been developed with Europe, North America, Japan. At the same time, being recognized the important of green roofs in domestic industry and we just start to develop the relative technology as analyzing the future direction of advanced technology. Recently, local self-government including Seoul support the system for green roof which provide 50% of working expenses form of Matching Fund by Seed Money. As years go by, the number of build up the green roof has gradually increased to be proved. At this result caused by management of system in government, unstructured construction system, low development of support technology. In this study as analyzing the present of technical development, supporting by law and system for the advanced waterproofing and root penetration resistance technology, we suggest the development direction of it to be compared with application advanced technology and we could strengthen the international competitiveness to be industrialized the green roof considering system, technology, human infrastructure.

• 한국산림과학회지
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• 제66권1호
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• pp.45-53
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• 1984

본(本) 연구(硏究)는 경사부위별(傾斜部位別) 토양별(土梁別)로 토양(土壤)의 물리적(物理的) 성질(性質) 중(中) 용적량(容積量)과 공극률(孔隙率)이 은수원사시나무의 생장(生長)과 근계발달(根系發達)에 미치는 영향(影響)을 분석(分析)한 것으로서 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) 용적중(容積重)과 공극률(空隙率)은 경사부위(傾斜部位)와 밀접(密接)한 관계를 가지고 있으며, 토심(土深)에 따라서 유의적(有意的)인 차이(差異)를 갖는다. 2) 용적중(容積重)은 하부(下部)에서 평균(平均) $1.17g/cm^3$(1.05~1.40), 상부(上部)에서 평균(平均) $1.43g/cm^3$(1.36~1.60)이었으며, 공극률(空隙率)은 하부(下部)에서 평균(平均) 52.65%(55.05~45.50), 상부(上部)에서 평균(平均) 41.20%(43.81~37.21)이었다. 3) 용적중(容積重)은 수고(樹高), 흉고직경(胸高直徑), Total biomass 및 임목(林木)의 각(各) 부위별(部位別) Biomass와 회귀분석결과(回歸分析結果) 부(負)의 상관(相關)을 나타내었다. 4)은수원사시나무의 근계(根系)는 천근성(淺根性)으로서 주로 Al속(屬)에 분포(分布)하였으며, 한계용적중(限界容積重)은 $1.40g/cm^3$이었다.

• Journal of Forest and Environmental Science
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• 제18권1호
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• pp.1-6
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• 2001

본 연구는 음나무의 증식을 위해 근삽을 이용하여 대량번식방법의 체계를 확립하고자 실시하였다. 조사된 결과는 다음과 같다. 1. 음나무의 근삽시 생장조절물질의 처리는 고농도보다 저농도가 효과적인 것으로 나타났으며, 생장조절물질 처리에 따른 부정아 발생율은 IBA, NAA 300mg/L 처리구에서 96.6%로 가장 높게 나타났으나 무처리 역시 90.0%로 높은 부정아 발생율을 나타내었다. 또한 엽의 특성(엽수, 엽신, 엽폭)은 무처리와 생장조절물질 처리간에 큰 차이를 나타내지 않았다. 2. 근의 방향에 따른 근삽은 가로근삽(100%)과 세로근삽 (92.5%) 이 모두 90% 이상의 부정아 발생율을 보여 두 처리 모두 높은 부정아 발생율을 나타내었고, 부정아 발생수 및 엽 특성(엽수, 엽병, 엽신 및 엽폭)에 있어서 세로근삽이 가로근삽보다 모두 높게 나타났다. 3. 바로묻기 한 것이 거꾸로묻기 한 것보다 부정아 발생율, 부정아 발생수 및 엽특성(엽수, 엽신, 엽폭)에서 높은 값을 나타냈으며, 거꾸로묻기 한 경우 세근의 발달이 없거나 미약하며, 부정아 발생의 경우 기극 부근에서 발생하여 지면으로 올라오는 경우를 나타냈다.